BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0065.p (381 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310800|ref|YP_003774892.1| tartrate dehydrogenase [Herbasp... 653 0.0 gi|300312594|ref|YP_003776686.1| 3-isopropylmalate dehydrogenase... 179 3e-46 gi|300310728|ref|YP_003774820.1| isocitrate dehydrogenase [Herba... 122 3e-29 >gi|300310800|ref|YP_003774892.1| tartrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 653 bits (1685), Expect = 0.0, Method: Compositional matrix adjust. Identities = 314/359 (87%), Positives = 334/359 (93%), Gaps = 1/359 (0%) Query: 24 MTK-HKIAVIAGDGIGQEVIPEGLRAVDAAAKKFGIEIEYTHFDWAHCGYYAEHGKMMPD 82 M+K H+IAVIAGDGIGQEV+PEGLRAV AA+KKFGI+IE+THFDWAHC YYAEHGKMMPD Sbjct: 1 MSKTHRIAVIAGDGIGQEVMPEGLRAVQAASKKFGIDIEFTHFDWAHCNYYAEHGKMMPD 60 Query: 83 DWFDQLKGFEAIFFGAVGWPATVPDHVSLRGSLLKFRRDFDLYVNLRPVRLMPGVPCPLA 142 DWF+QLKGFEAI+FGAVGWPATVPDHVSL GSLLKFRRDFDLYVNLRPVRLMPGVPCPLA Sbjct: 61 DWFEQLKGFEAIYFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLA 120 Query: 143 GRKPGDIDFYVVRENTEGEYSSIGGRMYEGTDREIVIQESVFTRHGVDRILKFAYDLAQS 202 RKPGDIDFYVVRENTEGEYSSIGGRMYEGT+RE VIQESVF+R G DRILKFA+DLAQS Sbjct: 121 NRKPGDIDFYVVRENTEGEYSSIGGRMYEGTERETVIQESVFSRKGTDRILKFAFDLAQS 180 Query: 203 RPKKHLTSATKSNGIAITMPYWDERVEAMGKQYGDVKTDKYHIDILTAHFVLNPQRFDVV 262 RPKKHLTSATKSNGIAITMPYWDERVEAMG +Y DV+ DKYHIDILTAHFVLNP RFDVV Sbjct: 181 RPKKHLTSATKSNGIAITMPYWDERVEAMGAKYPDVRRDKYHIDILTAHFVLNPDRFDVV 240 Query: 263 VAPNLFGDILSDLGPACAGTIGIAPSANLNPDRKLPSLFEPVHGSAPDIAGKNIANPVAT 322 VA NLFGDILSDLGPACAGTIGIAPSAN+NP R+LPSLFEPVHGSAPDI GKNIANPVA Sbjct: 241 VASNLFGDILSDLGPACAGTIGIAPSANINPTRELPSLFEPVHGSAPDIFGKNIANPVAM 300 Query: 323 IWSGAMMLDFLGNGNPKYKQAHDAILAAIEKVLVEGPKTPDLGGSGDTTSVGKAIAAAI 381 IWSGAMMLDFLG+G KY+QAHDA+LAAIE VLV GP TPDLGG +T VG+AIAAAI Sbjct: 301 IWSGAMMLDFLGDGQGKYRQAHDAMLAAIETVLVNGPTTPDLGGKSNTAEVGQAIAAAI 359 >gi|300312594|ref|YP_003776686.1| 3-isopropylmalate dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 179 bits (455), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 135/369 (36%), Positives = 192/369 (52%), Gaps = 30/369 (8%) Query: 28 KIAVIAGDGIGQEVIPEGLRAVDAAAKKFGIEIEYTHFDWAHCGYYAEHGKMMPDDWFDQ 87 KIA++ GDGIG E++ E +R ++A +KF +E GY A HG +PD Sbjct: 2 KIAILPGDGIGPEIVNEAVRVLNALDEKFEMETAPV----GGAGYEA-HGHPLPDGTLKL 56 Query: 88 LKGFEAIFFGAVG-WPATVPDHVSLR--GSLLKFRRDFDLYVNLRPVRLMPGV--PCPLA 142 K +AI FGAVG W + +LR ++L R+ L+ N RP P + L Sbjct: 57 AKEADAILFGAVGDWKYDKLER-ALRPEQAILGLRKHLQLFANFRPAICYPELTGASSLK 115 Query: 143 GRKPGDIDFYVVRENTEGEYSSIGGRMYEGTD------REIVIQESVFTRHGVDRILKFA 196 ++D +VRE Y M E D RE ++ + RI A Sbjct: 116 PELVANLDILIVRELNGDIYFGQPRGMREAPDGPFKGARE-GFDTMRYSEPEIRRIAHVA 174 Query: 197 YDLAQSRPKKHLTSATKSNGIAITMPYWDERVEAMGKQYGDVKTDKYHIDILTAHFVLNP 256 + A R K+ L S K+N + T +W + V +GK+Y DV+ ++D V P Sbjct: 175 FQAAAKRGKR-LCSVDKAN-VLETFQFWKDIVTEVGKEYPDVELSHMYVDNAAMQLVKAP 232 Query: 257 QRFDVVVAPNLFGDILSDLGPACAGTIGIAPSANLNPDRKLPSLFEPVHGSAPDIAGKNI 316 + FDV+V N+FGDILSD G+IG+ PSA+L+ + K L+EP HGSAPDIAG+ I Sbjct: 233 KNFDVIVTGNMFGDILSDAAAMLTGSIGMLPSASLDANNK--GLYEPSHGSAPDIAGRGI 290 Query: 317 ANPVATIWSGAMMLDFLGNGNPKYKQAHDAILAAIEKVLVEGPKTPDLGGSG----DTTS 372 ANP+ATI S AMML F N K +QA D I A++KVL +G +T D+ G +T Sbjct: 291 ANPLATILSAAMMLRFSLN---KAEQA-DRIENAVKKVLAQGLRTADIYEEGCTKVNTQQ 346 Query: 373 VGKAIAAAI 381 +G+A+ A+ Sbjct: 347 MGEAVVKAL 355 >gi|300310728|ref|YP_003774820.1| isocitrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 418 Score = 122 bits (307), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 107/365 (29%), Positives = 167/365 (45%), Gaps = 51/365 (13%) Query: 29 IAVIAGDGIGQEVIPEGLRAVDAAAKKFGIEIEYTHFDWAHCGYYAEHGKMMPDDWF-DQ 87 I I GDG+G +V P L+ V+AA +K H+ + G A PD W ++ Sbjct: 31 IPYIVGDGVGVDVTPVMLKVVNAAVEKAYGGARKIHWMEIYAGEKATR-LYGPDVWLPEE 89 Query: 88 LKGFEAIFFGAVGWPATVPDHVSLRGSLLKFRRDFDLYVNLRPVRLMPGVPCPLAGRKPG 147 + A+ P + P +R + R+ DLYV LRPVR GVP PL R+P Sbjct: 90 TLAVLKKYLVAIKGPLSTPVGGGIRSLNVAMRQQLDLYVCLRPVRYFKGVPSPL--REPE 147 Query: 148 DIDFYVVRENTEGEYSSIG--------GRMYEGTDREIVIQESVF-----------TRHG 188 D + REN+E Y+ I ++ + RE+ +++ F +R G Sbjct: 148 KTDMVIFRENSEDIYAGIEWAAGTPEVNKLIDLLTREMGVKKLRFPESSALGIKPVSREG 207 Query: 189 VDRILKFAYDLAQSRPKKHLTSATKSNGIAITM-PYWDERVEAMGKQYG----------- 236 +R+++ A A K +T K N + T + D +++G Sbjct: 208 TERLVRQAIQYAIDHDKPSVTLVHKGNIMKFTEGAFRDWGYALAAREFGAELIDDGPWMR 267 Query: 237 --DVKTDKYHI--DILTAHF----VLNPQRFDVVVAPNLFGDILSDLGPACAGTIGIAPS 288 + KT + I D +T F ++ P + V+ NL GD +SD A G IGIAP Sbjct: 268 LKNPKTGRAIIIKDAITDAFFQQVLMRPAEYSVIATLNLNGDYISDAVAAQVGGIGIAPG 327 Query: 289 ANLNPDRKLPSLFEPVHGSAPDIAGKNIANPVATIWSGAMMLDFLGNGNPKYKQAHDAIL 348 AN++ ++FE HG+AP AGK+ NP ++I S MML +G + +A D I+ Sbjct: 328 ANMSDSV---AVFEATHGTAPKYAGKDYVNPGSSILSAEMMLRHMG-----WIEAADLII 379 Query: 349 AAIEK 353 +A++K Sbjct: 380 SAMQK 384 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,330,439 Number of extensions: 61327 Number of successful extensions: 156 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 4 Length of query: 381 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 294 Effective length of database: 1,207,925 Effective search space: 355129950 Effective search space used: 355129950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)