BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0052.p (180 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313000|ref|YP_003777092.1| AMP-binding enzyme family prote... 217 4e-58 >gi|300313000|ref|YP_003777092.1| AMP-binding enzyme family protein [Herbaspirillum seropedicae SmR1] Length = 586 Score = 217 bits (553), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 114/180 (63%), Positives = 135/180 (75%), Gaps = 2/180 (1%) Query: 1 VLDQQGKLALNNRLRAVLADAIESVALPRLWRYLDALPANPQGKTTVAALTALLE-PTPS 59 +L QGKLALN L+AVLA AIES+ALPRLWRYL ALP NPQGK T++ALTALLE P + Sbjct: 408 MLAAQGKLALNTALKAVLAGAIESIALPRLWRYLPALPVNPQGKVTLSALTALLESPAAA 467 Query: 60 PLSTKRPLWPGQTVLQRGEHSVVLQLHVPPDLLYFDGHFPEAPILPGVVQLDWALALGRQ 119 P+ + P P + L+R V L L +P DLLYF GHFP +PILPGVVQ DW+LALGR+ Sbjct: 468 PVVSA-PTLPQVSELERSSEHVQLALDIPADLLYFRGHFPGSPILPGVVQTDWSLALGRR 526 Query: 120 YFDLPAHFRALQALKFQRVVTPGATLTLELENDTQKNTLAFRLHSANGQHASGRILFGEA 179 YFDLP HF LQALKFQRV+TPG + L+L+ + K+ L+FRLHS GQHASGR L G A Sbjct: 527 YFDLPPHFLGLQALKFQRVITPGMRVLLDLQFEAGKSQLSFRLHSEGGQHASGRFLLGAA 586 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 590,992 Number of extensions: 24746 Number of successful extensions: 89 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 89 Number of HSP's successfully gapped: 1 Length of query: 180 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 101 Effective length of database: 1,245,805 Effective search space: 125826305 Effective search space used: 125826305 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)