BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0043.p (134 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313007|ref|YP_003777099.1| hypothetical protein Hsero_3713... 204 2e-54 >gi|300313007|ref|YP_003777099.1| hypothetical protein Hsero_3713 [Herbaspirillum seropedicae SmR1] Length = 189 Score = 204 bits (519), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 98/133 (73%), Positives = 111/133 (83%) Query: 1 VERAGRTDDIDTVLEIEPDHLDLVGLAFGQRVMSLHYDGKELKTWRHFMLPKEVQGEDVL 60 VER R D++DT LEI+ +HLDLVGLAFGQRVMSLH+DG++L+ WRH +LP +VQGEDVL Sbjct: 56 VERGSRIDELDTALEIDAEHLDLVGLAFGQRVMSLHFDGRQLQVWRHALLPSQVQGEDVL 115 Query: 61 QDVQLTLWPAAAIRTALPPGWELEEDGMQRTLLLHREVIAQIDYPDRKAWGGKVILTNLR 120 QDVQLTLWPA AIR ALP GWEL E QRTLLLH E IA IDYPD++ WGG VIL NLR Sbjct: 116 QDVQLTLWPAEAIRQALPEGWELREAQAQRTLLLHGEKIAVIDYPDQRRWGGTVILNNLR 175 Query: 121 YRYKLTIQSVVTA 133 Y YKLTIQSVVT+ Sbjct: 176 YHYKLTIQSVVTS 188 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 464,354 Number of extensions: 19023 Number of successful extensions: 45 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 45 Number of HSP's successfully gapped: 1 Length of query: 134 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 58 Effective length of database: 1,260,010 Effective search space: 73080580 Effective search space used: 73080580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)