BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0041.p (113 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 115 7e-28 gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogena... 58 2e-10 gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogena... 57 3e-10 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 115 bits (289), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 55/70 (78%), Positives = 62/70 (88%) Query: 1 MTTTGNNTILVTGSSRGIGKAIALRLARDGYDIVLHCHSRRDAADAVAKEIEALGRSTRV 60 MTTTG ++I VTGSSRGIG+AIALRLAR+GYDIVLHCH+RRDAAD VA EI ALGR RV Sbjct: 1 MTTTGAHSIFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRV 60 Query: 61 LQFDIADRAQ 70 LQFD+A+R Q Sbjct: 61 LQFDVANREQ 70 Score = 67.8 bits (164), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 32/36 (88%), Positives = 34/36 (94%) Query: 77 ARRAGKPEEVASAVSFLMQEDAAYITRQVISVNGGL 112 ARRAGKPEEVA+AV+FLM DAAYITRQVISVNGGL Sbjct: 209 ARRAGKPEEVAAAVAFLMSPDAAYITRQVISVNGGL 244 >gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 57.8 bits (138), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 37/101 (36%), Positives = 57/101 (56%), Gaps = 2/101 (1%) Query: 1 MTTTGNNTI-LVTGSSRGIGKAIALRLARDGYDIVLHCHSRRDAADAVAKEIEALGRSTR 59 MT+ + I LVTG++R IG+ IAL LA+ G+D+ +H + R+ AD + +EIE LGR Sbjct: 1 MTSPSSAGIALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIETLGRRAL 60 Query: 60 VLQFDIADRAQT-XRRARARRAGKPEEVASAVSFLMQEDAA 99 +Q ++ D Q +A G+ V + S +DAA Sbjct: 61 AVQAELGDETQVRSLLPQAAPLGRISCVVNNASLFDYDDAA 101 >gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 57.4 bits (137), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 31/70 (44%), Positives = 42/70 (60%) Query: 10 LVTGSSRGIGKAIALRLARDGYDIVLHCHSRRDAADAVAKEIEALGRSTRVLQFDIADRA 69 L+TG SRG+GK AL LARDG ++L + AA+A E++ALG L+ D+AD A Sbjct: 12 LITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQALGAVAAALRLDLADSA 71 Query: 70 QTXRRARARR 79 A+A R Sbjct: 72 SFPAFAQAVR 81 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 271,281 Number of extensions: 8098 Number of successful extensions: 124 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 4 Length of query: 113 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 40 Effective length of database: 1,274,215 Effective search space: 50968600 Effective search space used: 50968600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 124 (52.4 bits)