BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0018.p (414 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 345 3e-96 gi|300311163|ref|YP_003775255.1| hypothetical protein Hsero_1837... 313 1e-86 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 189 4e-49 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 146 2e-36 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 138 8e-34 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 345 bits (886), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 189/257 (73%), Positives = 211/257 (82%), Gaps = 2/257 (0%) Query: 1 MSQMEGVGHLANWQWLFILEGIPSLIAGVFVLLYLPEKPANAKWLSVAEQQAVTQALVAE 60 MS MEGVGHLANWQWLFILEGIPS++AG VL +LPE+PANAKWLS AE+ AVTQ L AE Sbjct: 179 MSGMEGVGHLANWQWLFILEGIPSILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAE 238 Query: 61 NTSASKHLSFKDACSNYRVWLCAAVYFCVVSGNATIAFWTPSIIKEIGIKGNLQIGLISA 120 N++ KH SFKDAC+NYRVW+CAAVYFCVVSGNATIAFW PSIIKEIG+KGNLQIGLISA Sbjct: 239 NSAPGKHFSFKDACTNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISA 298 Query: 121 IPFIAGTLAMIWNGIHSDKTGERRMHSAIATLIASVGLILTGLTLGNAVMALCALTLAAI 180 IPFIAGTLAMIWNG HSDKTGERR+HSAIATLIA +GL+ TG+TLGNA +ALC+LTLAAI Sbjct: 299 IPFIAGTLAMIWNGFHSDKTGERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAI 358 Query: 181 GILAAFPVFWSIPAAFLAGTAAAGGIALINSXGRDLAAIEHHIEELAALGIPRPSAVPLY 240 GILAAFPVFWSIP AFLAGTAAAGGIALINS G + ++ + AL S Sbjct: 359 GILAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYM--IGALKTSTGSLSSGL 416 Query: 241 YRVASNQLTQSATVQVL 257 Y VA+ +L S V V Sbjct: 417 YFVAALELVASFLVVVF 433 >gi|300311163|ref|YP_003775255.1| hypothetical protein Hsero_1837 [Herbaspirillum seropedicae SmR1] Length = 225 Score = 313 bits (803), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 156/202 (77%), Positives = 169/202 (83%), Gaps = 3/202 (1%) Query: 213 GRDLAAIEHHIEELAALGIPRPSAVPLYYRVASNQLTQSATVQVLGGESSGEVETFVFNA 272 GRD+AAIEHHIEEL ALGI RPSAVPL+YRV++NQL+Q+A VQVLG SSGEVETFVFNA Sbjct: 27 GRDMAAIEHHIEELEALGISRPSAVPLFYRVSANQLSQAAQVQVLGEASSGEVETFVFNA 86 Query: 273 GGELYVSIASDHTDRKLEAHSVAFSXTDRKLEAHSVAPVAWKLADVAAYWDELVIRSWIK 332 GGELYVSIASDHTDRKLEAHSVAFS ++ V AWKLADVA YWDELVIRSWI+ Sbjct: 87 GGELYVSIASDHTDRKLEAHSVAFS---KQACVKPVGTTAWKLADVAEYWDELVIRSWIE 143 Query: 333 ENGNEVLYQEGTLASLRTPQDLIAGFTGGKDILPAGSGMTCGTVGAIGGIRPATSFTMEL 392 E+G LYQEG LASLRTP DLI FT G+ LP G GMTCGTVGAIGGIRPA SFTMEL Sbjct: 144 EDGQSKLYQEGPLASLRTPADLIERFTEGRAQLPEGCGMTCGTVGAIGGIRPAASFTMEL 203 Query: 393 FDPRRQRSLRHTYEAEILAEVA 414 FDPRRQR+LRH Y A+ LAEVA Sbjct: 204 FDPRRQRALRHHYVAQSLAEVA 225 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 189 bits (480), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 99/213 (46%), Positives = 135/213 (63%), Gaps = 1/213 (0%) Query: 1 MSQMEGVGHLANWQWLFILEGIPSLIAGVFVLLYLPEKPANAKWLSVAEQQAVTQALVAE 60 M M GV WQW+FILE IPSLI GV V+LY+ ++ +A WLS E++ + +A ++ Sbjct: 175 MHAMPGVWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLS-EEEKRLLEANISH 233 Query: 61 NTSASKHLSFKDACSNYRVWLCAAVYFCVVSGNATIAFWTPSIIKEIGIKGNLQIGLISA 120 S +HLS +N RVWL A +YFC V G ++FW P+IIK G+ L +GL++A Sbjct: 234 EESQKEHLSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTA 293 Query: 121 IPFIAGTLAMIWNGIHSDKTGERRMHSAIATLIASVGLILTGLTLGNAVMALCALTLAAI 180 IP+ AM+ G +D ERR H AI I +GL+L+ N +A+ +LTLA++ Sbjct: 294 IPYAFAAAAMVLIGRSADARRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASV 353 Query: 181 GILAAFPVFWSIPAAFLAGTAAAGGIALINSXG 213 GIL P+FWS+P AFL+GTAAA GIALINS G Sbjct: 354 GILTTLPLFWSLPTAFLSGTAAAAGIALINSLG 386 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 146 bits (369), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 89/209 (42%), Positives = 125/209 (59%), Gaps = 4/209 (1%) Query: 8 GHLANWQWLFILEGIPSLIAGVFVLLYLPEKPANAKWLSVAEQQAVTQALVAENTSA--S 65 G L WQW+F++E IP+++ GV +LYL +AKWL+ E++A QA + + S Sbjct: 183 GGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLN-DEEKASLQADIDGDAKGKES 241 Query: 66 KHLSFKDACSNYRVWLCAAVYFCVVSGNATIAFWTPSIIKEIGIKGNLQIGLISAIPFIA 125 KH F + RVWL +YF V G + W P+++K G+KGNL+IGL+SAIPF Sbjct: 242 KH-GFGAIVKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGC 300 Query: 126 GTLAMIWNGIHSDKTGERRMHSAIATLIASVGLILTGLTLGNAVMALCALTLAAIGILAA 185 +AM G +D+ ERR H I L+ VG + L N +++ +L+LAA G+L Sbjct: 301 AIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTC 360 Query: 186 FPVFWSIPAAFLAGTAAAGGIALINSXGR 214 P+FWS+P AFL+G AAA GIA INS G Sbjct: 361 APLFWSLPTAFLSGAAAAVGIAAINSVGN 389 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 138 bits (348), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 80/215 (37%), Positives = 130/215 (60%), Gaps = 4/215 (1%) Query: 1 MSQMEGVGHLANWQWLFILEGIPSLIAGVFVLLYLPEKPANAKWLSVAEQQAVTQALVAE 60 + ++ G+ WQ L +LE +P+++ GV L LP++PA AKWLS E+Q + L E Sbjct: 177 LMELHGMWGFKGWQVLLLLEALPAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTE 236 Query: 61 -NTSASKHLS-FKDACSNYRVWLCAAVYFCVVSGNATIAFWTPSIIKEIGIKGNLQIGLI 118 N A++H + KDA +N+RV+ A FC + G+ +I W P II+E G+ + Q+GL+ Sbjct: 237 QNVLAARHGNKLKDAFTNWRVFALAGANFCGIIGSLSIGLWLPQIIREFGLPSH-QVGLV 295 Query: 119 SAIPFIAGTLAMIWNGIHSDKTGERRMHSAIATLIASVGLILTGLTLGNAVMALCALTLA 178 +AIP++ G +AM ++++ R A A ++A++ L ++ L ++ + A+T+A Sbjct: 296 AAIPYLVGAVAMTLWARLANRSERRLFFVAGAIVLAALSLGISAF-LHTPLLKMLAITVA 354 Query: 179 AIGILAAFPVFWSIPAAFLAGTAAAGGIALINSXG 213 IL+ FW+IP+ FL G AAAGG+ALI S G Sbjct: 355 VASILSFQATFWAIPSGFLTGRAAAGGLALIVSIG 389 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,183,121 Number of extensions: 46353 Number of successful extensions: 175 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 10 Length of query: 414 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 326 Effective length of database: 1,203,190 Effective search space: 392239940 Effective search space used: 392239940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)