BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0014.p (328 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 308 5e-85 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 116 2e-27 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 110 1e-25 gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 108 4e-25 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 100 3e-22 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 308 bits (788), Expect = 5e-85, Method: Compositional matrix adjust. Identities = 166/296 (56%), Positives = 202/296 (68%), Gaps = 9/296 (3%) Query: 20 DILRRTAAEAELVRRNSELTELNRKLSMTQEQLMQSEKLASIGQLAAGVAHEINNPIGYI 79 DI R E L R+ E +L +L M Q QLMQSEKLASIGQLAAGVAHEINNPIG++ Sbjct: 146 DITGRKEMEDTLQRQAEEQRKLIEQLKMAQAQLMQSEKLASIGQLAAGVAHEINNPIGFV 205 Query: 80 FSNFSTLETYVGSLLRMLGAYEAQELDRGSESAA---RELAAVRDEVEIDFLKEDIPALM 136 +N +TL YV +LL G Q L G+ AA +L + ++ FL ED+ L+ Sbjct: 206 SANLNTLGNYVATLLE--GIQLHQCLQGGALPAAMAQEQLRSFEARADLAFLSEDVTELL 263 Query: 137 SESREGITRVRKIVQDLKDFSRADTALEWQWANLHQGIDSTLNIVNNEIKYKADVVKNYG 196 +ES EG+ RVR IVQ LKDFSR + WQ +LH+G+DSTLNI NNE+KYK +VK YG Sbjct: 264 AESNEGLQRVRNIVQSLKDFSRIGES-NWQMTDLHRGLDSTLNIANNELKYKVSIVKQYG 322 Query: 197 VLPDVECLPSQINQVVMNLVVNAAHAIDGARGTITITTRIVDTGADVEIEIADNGSGITP 256 LP V+CL S++NQV MNL+VNAA AI RGT+TI T G V + I+D G GI P Sbjct: 323 ELPLVKCLASELNQVFMNLLVNAAQAIS-ERGTVTICT--ARAGDKVSVSISDTGHGIAP 379 Query: 257 ENLSRIFDPFFTTKPVGQGTGLGLSLAYGIMQKHHGSINVRSELGAGTTFTLRFPI 312 ENL+RIF+PFFTTKPVG GTGLGLSL+YGI++KH G I V S G GTTFT+ PI Sbjct: 380 ENLNRIFEPFFTTKPVGSGTGLGLSLSYGIVKKHGGDIRVSSAPGCGTTFTIELPI 435 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 116 bits (291), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 93/298 (31%), Positives = 140/298 (46%), Gaps = 52/298 (17%) Query: 36 SELTELNRKLSMTQEQLMQSEKLASIGQLAAGVAHEINNPIGYIFSNFSTLETYVGSLLR 95 +E++ R+L T+E L S+K+ +IGQL G+AH+ NN +G I + + +R Sbjct: 443 AEVSARTRELMATEEALRHSQKIEAIGQLTGGLAHDFNNLLGGISGSLDLMR------VR 496 Query: 96 MLGAYEAQELDRGSESAARELAAVRDEVEIDFLKEDIPALMSESREGITRVRKIVQDLKD 155 ++ +EL R E AAR R + Q L Sbjct: 497 LVQG-RGEELLRYVELAARS---------------------------TQRAASLTQRLLA 528 Query: 156 FSRADTALEWQWANLHQGIDSTLNIVNNEI--KYKADVVKNYGVLPDVECLPSQINQVVM 213 F+R T L + ++ I ++ I +VV G+ P P Q+ ++ Sbjct: 529 FARRQT-LAPKPTRVNALISGMEELIRRTIGPAIVLEVVGAGGLWPTFVDGP-QLESALL 586 Query: 214 NLVVNAAHAIDGARGTITITT------------RIVDTGADVEIEIADNGSGITPENLSR 261 NL +NA A+ G G +TI T R + G V I + D+G G+ P+ L+R Sbjct: 587 NLCINARDAMPGG-GRLTIETANKWLDDRWAHERDLPPGQYVCISVTDSGVGMPPDVLAR 645 Query: 262 IFDPFFTTKPVGQGTGLGLSLAYGIMQKHHGSINVRSELGAGTTFTLRFPIRHDAGGD 319 FDPFFTTKP+G GTGLGLS+ YG ++ G + + SE+G GTT ++ P RH GD Sbjct: 646 AFDPFFTTKPIGAGTGLGLSMVYGFARQSGGQVRLYSEVGQGTTVSIYLP-RHQHDGD 702 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 110 bits (275), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 93/312 (29%), Positives = 145/312 (46%), Gaps = 60/312 (19%) Query: 20 DILRRTAAEAELVRRNSELTE------LNRKLSMTQEQLMQSEKLASIGQLAAGVAHEIN 73 DI R AE L N L + L R S + L Q +K+ ++GQL GVAH+ N Sbjct: 681 DITERRRAEEALRELNDTLEQRVVEEILER--SRAEAALRQMQKMEAVGQLTGGVAHDFN 738 Query: 74 NPIGYIFSNFSTLET--YVGSLLRMLGAYEAQELDRGSESAARELAAVRDEVEIDFLKED 131 N + I N L++ + R +DRG++ +++ LA R + Sbjct: 739 NVLQIIGGNLQLLQSDPRLDERSRQRVRIAIGAVDRGAKLSSQLLAFARRQ--------- 789 Query: 132 IPALMSESREGITRVRKIVQDLKDFSRADTALEWQWANLHQGIDSTLNIVNNEIKYKADV 191 L S + ++VQ++ D R + ID + + ++ D Sbjct: 790 --PLQPRS----INLERVVQNMDDLLRRAVG---------ENIDISTRVAHDLWNTMLD- 833 Query: 192 VKNYGVLPDVECLPSQINQVVMNLVVNAAHAI-DGARGTITI-TTRIVDT---------- 239 P+Q+ +++N +NA A+ G R T+ I R+ D Sbjct: 834 -------------PNQVENLIINTAINARDAMPSGGRLTVQIDNVRLEDPVFLSQADLAP 880 Query: 240 GADVEIEIADNGSGITPENLSRIFDPFFTTKPVGQGTGLGLSLAYGIMQKHHGSINVRSE 299 G VE++I+DNG+G++PE R F+PFFTTK G+GTGLGLS+AYG +++ G I++ SE Sbjct: 881 GEYVELQISDNGAGMSPEVRDRAFEPFFTTKLEGEGTGLGLSMAYGFVKQSKGHISIHSE 940 Query: 300 LGAGTTFTLRFP 311 G GTT + FP Sbjct: 941 QGKGTTIRIYFP 952 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 108 bits (271), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 98/322 (30%), Positives = 144/322 (44%), Gaps = 67/322 (20%) Query: 20 DILRRTAAEAELVRRNSELTELNRKLSM-----------TQEQLMQSEKLASIGQLAAGV 68 DI R A EAE R + L LN L +E L Q++K+ ++GQL G+ Sbjct: 258 DIEGRRAVEAERDRALAALRTLNETLEQRVEERTAALLNAEEALRQAQKMEAVGQLTGGL 317 Query: 69 AHEINNPIGYIFSNFSTLETYVGSLLRMLGAYEAQELDRGSESAARELAAVRDEVEIDFL 128 AH+ NN I + S GSL EL VR L Sbjct: 318 AHDFNN----ILAGIS------GSL---------------------ELMKVR-------L 339 Query: 129 KE----DIPALMSESREGITRVRKIVQDLKDFSRADTALEWQWANLHQGIDSTLNIVNNE 184 + DI ++ ++ + R + Q L FSR T L+ + A+L++ + ++ Sbjct: 340 AQGRIGDIERHLNGAQSAVKRAAALTQRLLAFSRRQT-LDPKPADLNRIVAGMHELIGRS 398 Query: 185 IKYKADV-VKNYGVLPDVECLPSQINQVVMNLVVNAAHAID-GARGTITITTRIVD---- 238 + V G L Q+ ++NL +NA A+ G R TI R +D Sbjct: 399 VGPAIAVETIAAGGLWHTFVDIGQMENALLNLCINARDAMPHGGRLTIETANRWLDDIGA 458 Query: 239 ------TGADVEIEIADNGSGITPENLSRIFDPFFTTKPVGQGTGLGLSLAYGIMQKHHG 292 G V + ++D G+G++PE ++R FDPFFTTKP GQGTGLGLS+ YG + G Sbjct: 459 LQRGVPPGQYVSLSVSDTGAGMSPEVVARAFDPFFTTKPTGQGTGLGLSMVYGFAGQSGG 518 Query: 293 SINVRSELGAGTTFTLRFPIRH 314 ++ + SELG G + P RH Sbjct: 519 TVRIYSELGQGAMVCIYLP-RH 539 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 99.8 bits (247), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 81/287 (28%), Positives = 134/287 (46%), Gaps = 54/287 (18%) Query: 49 QEQLMQSEKLASIGQLAAGVAHEINNPIGYIFSNFSTLETYVGSLLRMLGAYEAQELDRG 108 ++ L Q+ K+ ++GQL G+AH+ NN + I + + S + +L R Sbjct: 164 EQALGQARKMEALGQLTGGIAHDFNNLLQVITGYLDMINLALRS--------DKPDLARV 215 Query: 109 SESAARELAAVRDEVEIDFLKEDIPALMSESREGITRVRKIVQDLKDFSRADTALEWQWA 168 + SA D + R+ + + Q L F+R LE + Sbjct: 216 ARSA--------DSI----------------RKASGKAAMLTQQLLAFARKQR-LEGRTI 250 Query: 169 NLHQGIDSTLNIVNN----EIKYKADVVKNYGVLPDVECLPSQINQVVMNLVVNAAHAID 224 NL+ + ++V+ +I + ++ + L + P+Q+ ++N+++NA A++ Sbjct: 251 NLNSLTEGFTDLVSRTLGGDITVQTELARG---LWNCRLDPTQMEVALLNVLINARDAMN 307 Query: 225 GARGTITITT---------RIVDTGAD----VEIEIADNGSGITPENLSRIFDPFFTTKP 271 G RG + I T R V G V I + D+G GI P+ L R+ DPFFTTK Sbjct: 308 G-RGRVRIRTANEDLADPDRAVQLGLKEGLYVSIAVTDDGPGIAPDILPRVMDPFFTTKD 366 Query: 272 VGQGTGLGLSLAYGIMQKHHGSINVRSELGAGTTFTLRFPIRHDAGG 318 G+GTGLGLS+ YG +++ GS+ + S GTT T+ FP D G Sbjct: 367 EGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFPATQDQVG 413 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.132 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 900,875 Number of extensions: 35401 Number of successful extensions: 242 Number of sequences better than 1.0e-08: 23 Number of HSP's gapped: 238 Number of HSP's successfully gapped: 23 Length of query: 328 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 242 Effective length of database: 1,212,660 Effective search space: 293463720 Effective search space used: 293463720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)