BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0012.p (221 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312232|ref|YP_003776324.1| signal transduction protein [He... 127 5e-31 gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626... 117 8e-28 gi|300310564|ref|YP_003774656.1| regulator of the two-component ... 74 1e-14 gi|300311595|ref|YP_003775687.1| two component response regulato... 73 2e-14 >gi|300312232|ref|YP_003776324.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 127 bits (320), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 69/196 (35%), Positives = 110/196 (56%), Gaps = 4/196 (2%) Query: 27 LPALPNIVLELIHALDNKDVDTHTLAQKISYDQTLTAKVLRLANSSFYGMQSKIGSIPHA 86 LP++P +V E+I + +N+ V +A+K++ DQ L+AK+LRLANSS+Y + IG++ A Sbjct: 13 LPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYYHVSRTIGTVDDA 72 Query: 87 ITVLGFNSVRSLVLAAAVVDTFANHASEDLDHAHFWKHAIGTALCAKAIAVHRGANQDQS 146 + +LGF +VR+LV++ + +F DL FW++++ TAL AK +A N D + Sbjct: 73 VLMLGFMTVRTLVVSTGMAGSFKKLPGVDLK--LFWRYSLDTALVAKWLAKRGKLNSDFA 130 Query: 147 FIAGLLHNIGRLVLATCSPLRYRDVLQRCAQHDEDLLAAEIHVFGIDHKTAGKAVLEYWK 206 F GL+H IG+LV+ P + V + D L E FG D G + W+ Sbjct: 131 FTVGLMHAIGQLVMHAGMPEQALQVDKVAGPLDARRLDVERKSFGYDFADVGAELARRWR 190 Query: 207 FPXQLS--LMRSPTPL 220 FP + + + P PL Sbjct: 191 FPDEFAKVIEAFPQPL 206 >gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626 [Herbaspirillum seropedicae SmR1] Length = 290 Score = 117 bits (293), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 72/208 (34%), Positives = 111/208 (53%), Gaps = 20/208 (9%) Query: 25 SRLPALPNIVLELIHALDNKDVDTHTLAQKISYDQTLTAKVLRLANSSFYGMQSKIGSIP 84 +LP +P +V +L+ + + +D+ H +AQ+I+ D L+AKVLRLANS+F+ IG++ Sbjct: 11 GKLPTVPKVVQQLMASFNAEDISAHEIAQQIATDPALSAKVLRLANSAFFHASRTIGTVD 70 Query: 85 HAITVLGFNSVRSLVLAAAVVDTFANHASEDLDHAHFWKHAIGTALCAKAIAVHRGA--- 141 A+ +LGF VR+LVL +V F N + +D FW+H + TA+ A+ +A+H G Sbjct: 71 DALRILGFIMVRNLVLGNGMVAAFKN--TRGMDLHQFWRHTLYTAVAARWLAMHAGMVGE 128 Query: 142 -NQDQSFIAGLLHNIGRLVL-----ATCSPL-RYRDVLQRCAQHDEDLLAAEIHVFGIDH 194 N D F G++H IG+L L +PL R+ +VL D D E +G + Sbjct: 129 DNGDAVFTVGMMHGIGQLQLHAVAANAVAPLDRHSNVL------DSDRAEREKEAWGFHY 182 Query: 195 KTAGKAVLEYWKFPXQL--SLMRSPTPL 220 + W FP L +L P PL Sbjct: 183 ADVSAELAAIWHFPDALVTALRSVPDPL 210 >gi|300310564|ref|YP_003774656.1| regulator of the two-component system protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 73.9 bits (180), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 51/184 (27%), Positives = 85/184 (46%), Gaps = 3/184 (1%) Query: 27 LPALPNIVLELIHALDNKDVDTHTLAQKISYDQTLTAKVLRLANSSFYGMQSKIGSIPH- 85 LP+ + L ++ + D L + D L+ +++R+ N I S+ Sbjct: 19 LPSPGGVRLTIMRLCAQDNPDMAELIHLVQADPALSGRIIRIVNVLNAQRVRPIASVSMD 78 Query: 86 AITVLGFNSVRSLVLAAAVVDTFANHASEDLDHAHFWKHAIGTALCAKAIAVH-RGANQD 144 ++ ++G +VR L LA ++ +T A + + FW H+ A A+A+ R A Sbjct: 79 SLLLVGLQAVRQLALALSLAETRAPASCANFSLPAFWAHSFAMACSAQAVTSQLRVAPLG 138 Query: 145 QSFIAGLLHNIGRLVLATCSPLRYRDVLQRCAQHDEDLLAAEIHVFGIDHKTAGKAVLEY 204 + F GLL NIG+L LA+ P Y +L R A A E +FG +H A+++ Sbjct: 139 EMFTTGLLANIGKLTLASAQPTGYSALLTR-ALDAPARRAMEQSLFGFNHLNIAAALMQD 197 Query: 205 WKFP 208 WK P Sbjct: 198 WKIP 201 >gi|300311595|ref|YP_003775687.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 310 Score = 72.8 bits (177), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 47/184 (25%), Positives = 88/184 (47%), Gaps = 3/184 (1%) Query: 27 LPALPNIVLELIHALDNKDVDTHTLAQKISYDQTLTAKVLRLANSSFYGMQSKIGSIP-H 85 LPA + L+++ +D+ L ++ D L +++++AN + S+ Sbjct: 19 LPAPSGVRLKIMQMCQQEDIALPELVAQVQGDPVLAGRLIQIANIPNINKGRMVASVNVE 78 Query: 86 AITVLGFNSVRSLVLAAAVVDTFANHASEDLDHAHFWKHAIGTALCAKAIA-VHRGANQD 144 + ++G ++VR + LA ++ ++ + D+ FW + A +A+ + + A Sbjct: 79 LLLMVGLHAVRQMALAISLTESARRGDCAEFDYDAFWSRSTAMACAGQALGDLLQVAPPA 138 Query: 145 QSFIAGLLHNIGRLVLATCSPLRYRDVLQRCAQHDEDLLAAEIHVFGIDHKTAGKAVLEY 204 + F GLL IG+L LA+ P RY ++L A+ D L E FG DH A+++ Sbjct: 139 EMFTIGLLAGIGQLGLASARPKRYGELLLAGAK-DAQLRVQERESFGFDHLALAAAMMQD 197 Query: 205 WKFP 208 W FP Sbjct: 198 WHFP 201 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 652,167 Number of extensions: 24296 Number of successful extensions: 78 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 73 Number of HSP's successfully gapped: 4 Length of query: 221 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 139 Effective length of database: 1,231,600 Effective search space: 171192400 Effective search space used: 171192400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)