BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0052.p (523 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 507 e-144 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 152 7e-38 gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 119 4e-28 gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 65 1e-11 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 507 bits (1305), Expect = e-144, Method: Compositional matrix adjust. Identities = 258/329 (78%), Positives = 288/329 (87%), Gaps = 1/329 (0%) Query: 182 DPIHGFGAEVVLHGDTLEEARAHAFALAQERGLTFVHPYDDEAIIAGQGTVALEMLDAVP 241 D + G EVVL GD+ +A HA L +++ LTFVHP+DD +IAGQGTV +E+L P Sbjct: 108 DAVKARGGEVVLFGDSFTDAYEHALTLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHP 167 Query: 242 D-LDALVVAIGGGGLISGIAAYVKAVRPDIKIIGVQTTDSDAMARSIKAGRRVTLPDVGL 300 D + A+ VAIGGGGLI+G+A+YVKAVRPDIKIIGVQTTDSDAMARS+KAGRRV LPDVGL Sbjct: 168 DPIHAIFVAIGGGGLIAGVASYVKAVRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGL 227 Query: 301 FSDGTAVKLVGEETFRITRQLVDDIILVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAK 360 FSDGTAVKLVGEETFRI ++LVD++I+VDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAK Sbjct: 228 FSDGTAVKLVGEETFRIAKELVDEVIIVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAK 287 Query: 361 AYIERAKNSKKPIRNQSLITIACGANMNFDRLRFVAEMADVGEAREAVFAVTIPEEHGSF 420 AY+ERAK SKKPI+ +SLITIACGANMNFDRLRFVAEMAD GEAREAVFAVTIPEE GSF Sbjct: 288 AYVERAKASKKPIKGESLITIACGANMNFDRLRFVAEMADAGEAREAVFAVTIPEERGSF 347 Query: 421 RRFCEVIGPRNVTEFNYRISDAKSAHIFVGIQTGSADEPARIAKTFEKNGFSTLDLTHDE 480 RRFCE +GPRNVTEFNYRISDAK+AH+FVGIQ +ADE +IA+ FEK GF LDLTHDE Sbjct: 348 RRFCETVGPRNVTEFNYRISDAKAAHVFVGIQVSAADEAGKIARNFEKAGFGVLDLTHDE 407 Query: 481 LAKVHIRHLVGGKSTLAQDELLYRFEFPE 509 LAKVHIRHLVGGKS L DELLYRFEFPE Sbjct: 408 LAKVHIRHLVGGKSDLTGDELLYRFEFPE 436 Score = 335 bits (860), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 159/174 (91%), Positives = 166/174 (95%) Query: 1 MTTDYLQKILTARVYDVAMETPLEIANTLSQRMENRIYFKREDMQSVFSFKLRGAYNKMA 60 M+ DYL+KILTARVYDVA ETPLE+ANTLSQR++NRIYFKREDMQSVFSFKLRGAYNKMA Sbjct: 1 MSNDYLKKILTARVYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMA 60 Query: 61 HLPPAQLKRGVICASAGNHAQGVALSAAKLGCRAVIVMPTTTPSVKIEAVKGRGGEVVLF 120 HL PAQLKRGVICASAGNHAQGVALSAA+LGCRAVIVMPTTTP VKI+AVK RGGEVVLF Sbjct: 61 HLTPAQLKRGVICASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLF 120 Query: 121 GDSFTDAYNHALTLEKKLKLTFVHPFDDPYVIAGQGTVGMEILRQHADPIHAIF 174 GDSFTDAY HALTLEKK KLTFVHPFDDPYVIAGQGTVGMEILRQH DPIHAIF Sbjct: 121 GDSFTDAYEHALTLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIF 174 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 152 bits (384), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 152/472 (32%), Positives = 221/472 (46%), Gaps = 92/472 (19%) Query: 13 RVYDVAMETPLEIANTLSQRMENRIYFKREDMQSVFSFKLRGAYNKMAHLPPAQLKRGVI 72 R+ +ETP + TLSQ + +++ K E++Q SFK RGA NK+ + Sbjct: 13 RLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLVD----------V 62 Query: 73 CASAGNHAQGVALSAAKLGCRAVIVMPTTTPSVKIEAVKGRGGEVVLFGDSFTDAYNHAL 132 AS G G R V+ M G+ HA Sbjct: 63 AASGG-------------GARGVVAMSA--------------------GNHAQGVAYHAQ 89 Query: 133 TLEKKLKLTFVHPFDDPYVIAGQGTVGMEILRQHADPIHAIFXILRQHADPIHGFGAEVV 192 L L T V P P G++I R GFGA VV Sbjct: 90 RL--GLPATIVMPRFTP---------GVKIERTR-------------------GFGANVV 119 Query: 193 LHGDTLEEARAHAFALAQERGLTFVHPYDDEAIIAGQGTVALEMLDAVPDLDALVVAIGG 252 LHGDTLE AR HA+ LA++ GLTFVHPYDDEAIIAGQGTVALEM+ AVPDLD LVV+IGG Sbjct: 120 LHGDTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGG 179 Query: 253 GGLISGIAAYVKAVRPDIKIIGVQTTDSDAMARSIKAGRRVTLPDVG--LFSDGTAVKLV 310 GGL++G+A +A++P+I+I+GVQT M ++K T VG ++G AV Sbjct: 180 GGLLAGMAVAARAIKPEIEIVGVQTRRFPTMVNAVKG----TEHPVGASTIAEGIAVGTP 235 Query: 311 GEETFRITRQLVDDIILVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYIERAKNSK 370 G T I + D+++LVD + A+ + + +++VE AGA +A Y +R Sbjct: 236 GTLTRAIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDR----- 290 Query: 371 KPIRNQSLITIACGANMNFDRLRFVAEMADVGEAREAVFAVTIPEEHGSFRRFCEVIGP- 429 R + + + G N++ L + E V R A V+ + G+ R ++ Sbjct: 291 --FRGKRVGLVLSGGNIDPLLLTAIIERGMVRAGRLARVKVSSRDVPGTLARITGIVAEA 348 Query: 430 -RNVTEFNYR----ISDAKSAHIFVGIQTGSADEPARIAKTFEKNGFSTLDL 476 NV E +++ + A++ I + +QT A++ + GF +L Sbjct: 349 GANVDEVHHQRAFTLLAAQNVDIELVLQTRGRPHLAQVLQALRAAGFEAEEL 400 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 119 bits (299), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 65/162 (40%), Positives = 92/162 (56%), Gaps = 1/162 (0%) Query: 13 RVYDVAMETPLEIANTLSQRMENRIYFKREDMQSVFSFKLRGAYNKMAHLPPAQLKRGVI 72 R+ A TP+ + T + +++FK E+ Q + +FK RGA N + Q K GVI Sbjct: 22 RIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNALTQFDATQRKHGVI 81 Query: 73 CASAGNHAQGVALSAAKLGCRAVIVMPTTTPSVKIEAVKGRGGEVVLFGDSFTDAYNHAL 132 S+GNHAQG AL+A LG AVIVMP P+ K+ A +G G EVV + D Sbjct: 82 TFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVTYDRYTEDREAIGA 141 Query: 133 TLEKKLKLTFVHPFDDPYVIAGQGTVGMEILRQHADPIHAIF 174 L + K+T + P+D P+V+AGQGT +E+L Q P+ A+F Sbjct: 142 RLAAERKMTLIPPYDHPHVMAGQGTATLELL-QETGPLDALF 182 Score = 95.1 bits (235), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 71/207 (34%), Positives = 111/207 (53%), Gaps = 7/207 (3%) Query: 186 GFGAEVVLHGDTLEEARAHAFALAQERGLTFVHPYDDEAIIAGQGTVALEMLDAVPDLDA 245 G+GAEVV + E+ A LA ER +T + PYD ++AGQGT LE+L LDA Sbjct: 121 GYGAEVVTYDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDA 180 Query: 246 LVVAIGGGGLISGIAAYVKAVRPDIKIIGVQTTDSDAMARSIKAGRRVTLPDVGLFSDGT 305 L V +GGGGL+SG A +A+ P KI GV+ + +S++AG+ V + +DG Sbjct: 181 LFVCLGGGGLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGA 240 Query: 306 AVKLVGEETFRITRQLVDDIILVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYIER 365 + +G+ TF + R+LVDDI V + + ++ + + +VEP G L A A+ Sbjct: 241 QTQHLGKYTFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKL--- 297 Query: 366 AKNSKKPIRNQSLITIACGANMNFDRL 392 K ++ + + I G N++ +L Sbjct: 298 ----KDQLQGKRVGVIISGGNVDLGKL 320 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 65.5 bits (158), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 47/170 (27%), Positives = 85/170 (50%), Gaps = 1/170 (0%) Query: 178 RQHADPIHGFGAEVVLHGDTLEEARAHAFALAQERGLTFVHPYDDEAIIAGQGTVALEML 237 R+ + G E++ HG +E+R +A LA ++ L + P ++AG + +E+ Sbjct: 113 REKNAAMRALGVELIEHGSEFQESREYAQQLAAQQQLHMI-PSLHRDLVAGVASYWMELF 171 Query: 238 DAVPDLDALVVAIGGGGLISGIAAYVKAVRPDIKIIGVQTTDSDAMARSIKAGRRVTLPD 297 A P LD ++V IG G + G A A+ +IIGV + + A S +AGR++ P Sbjct: 172 AAQPGLDVVLVPIGQGSGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPV 231 Query: 298 VGLFSDGTAVKLVGEETFRITRQLVDDIILVDTDAVCTAIKDIFQDTRSI 347 +DG A ++ + + + VD+++ V D V A+K + T ++ Sbjct: 232 STCIADGVACRVPDQASLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNV 281 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 33/100 (33%), Positives = 53/100 (53%), Gaps = 1/100 (1%) Query: 29 LSQRMENRIYFKREDMQSVFSFKLRGAYNKMAHLPPAQLK-RGVICASAGNHAQGVALSA 87 L+Q + ++ K E+ +FK+RG + HL Q + GV+ A+ GNH Q V L+A Sbjct: 37 LNQALGAEVWVKHENHAPTGAFKVRGGMVYLHHLARQQPQLSGVVSATRGNHGQSVGLAA 96 Query: 88 AKLGCRAVIVMPTTTPSVKIEAVKGRGGEVVLFGDSFTDA 127 +LG A IV+P K A++ G E++ G F ++ Sbjct: 97 RRLGISATIVVPQGNSREKNAAMRALGVELIEHGSEFQES 136 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,440,200 Number of extensions: 56939 Number of successful extensions: 196 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 190 Number of HSP's successfully gapped: 8 Length of query: 523 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 433 Effective length of database: 1,193,720 Effective search space: 516880760 Effective search space used: 516880760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)