BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0034.p (1417 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313212|ref|YP_003777304.1| glutamate synthase large subuni... 2674 0.0 gi|300312905|ref|YP_003776997.1| glutamate synthase [Herbaspiril... 132 1e-31 >gi|300313212|ref|YP_003777304.1| glutamate synthase large subunit oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 1558 Score = 2674 bits (6932), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1305/1417 (92%), Positives = 1349/1417 (95%), Gaps = 15/1417 (1%) Query: 1 VTDALERKLYVIRKSSGHAIQALNLIHGKEFFVPSMSARTVVYKGLLLADQVGVYYKDLQ 60 VTDALERKLYVIRKSSGHAIQALNL+HGKEFFVPSMSARTVVYKGLLLADQVGVYYKDLQ Sbjct: 157 VTDALERKLYVIRKSSGHAIQALNLLHGKEFFVPSMSARTVVYKGLLLADQVGVYYKDLQ 216 Query: 61 DPRCVSALALVHQRFSTNTFPEWPLAHPYRLIAHNGEINTVKGNFNWMRAREGVMQSAVL 120 D RCVSALALVHQRFSTNTFPEWPLAHPYRLIAHNGEINTVKGNFNWMRAREGVMQSAVL Sbjct: 217 DERCVSALALVHQRFSTNTFPEWPLAHPYRLIAHNGEINTVKGNFNWMRAREGVMQSAVL 276 Query: 121 GDDLKKLFPLIYEGQSDTACFDNALELLLMAGYPLAQAMMMMVPEAWENHTLMDDNRRAF 180 G DLKKLFPLIYEGQSDTA FDNALELL+MAGYPL QAMMMMVPEAWENHT MDDNRRAF Sbjct: 277 GADLKKLFPLIYEGQSDTASFDNALELLVMAGYPLPQAMMMMVPEAWENHTSMDDNRRAF 336 Query: 181 YEYHAAMMEPWDGPAALAFTDGRHIGGTLDRNGLRPARYIVTDDDLVVMASESGVLPIPE 240 YEYHAAMMEPWDGPAALAFTDGRHIGGTLDRNGLRPARYIVTDDDLVVMASESGVLPIPE Sbjct: 337 YEYHAAMMEPWDGPAALAFTDGRHIGGTLDRNGLRPARYIVTDDDLVVMASESGVLPIPE 396 Query: 241 SKIIQKWRLQPGKMFLIDLDAGRIIDDKELKDTYANAKPYKQWISSVRVKLDELKAEPFE 300 SKIIQKWRLQPGKMFLIDLDAGRIIDDKELKDTYANAKPYKQWI+SVRVKLDELK EP + Sbjct: 397 SKIIQKWRLQPGKMFLIDLDAGRIIDDKELKDTYANAKPYKQWINSVRVKLDELKEEPRQ 456 Query: 301 SGSTIPLLDRQQVFGYTQEDVKFLMAPMASAGEEAIGSMGNDSPLAVMSNKNKTLYNYFK 360 S++ LLD QQVFGYTQEDVKFLMAPMAS GEEAIGSMGNDSPLAVMSNKNK LYNYF+ Sbjct: 457 PSSSLKLLDLQQVFGYTQEDVKFLMAPMASGGEEAIGSMGNDSPLAVMSNKNKPLYNYFR 516 Query: 361 QLFAQVTNPPIDPIREAMVMSLVSFIGPRPNLLDTNNINPPMRLEVSQPVLDYADIAKLR 420 QLFAQVTNPPIDPIREA+VMSLVSFIGP+PNLLDTNNINPPMRLEVSQPVLDY DIAKLR Sbjct: 517 QLFAQVTNPPIDPIREALVMSLVSFIGPKPNLLDTNNINPPMRLEVSQPVLDYDDIAKLR 576 Query: 421 NISAHTGGKFKSHELNICYPVAWGKEGIEARLASLCAKAVDAVKSGHNILIISDRKVDSE 480 NISAH+GGKFKS+ELNICYPVAWGKEGIEARLASLCAKAVDAVKSGHNILIISDRKVD+E Sbjct: 577 NISAHSGGKFKSYELNICYPVAWGKEGIEARLASLCAKAVDAVKSGHNILIISDRKVDAE 636 Query: 481 QVAIPALLATSAIHLHLVSKGLRTSTGLVVDTGSARETHHFALLAGYGAEAIHPYLAMDT 540 Q+ IPALLATSAIH HLVSKGLRTSTGLVV+TGSARETHHFALLAGYGAEAIHPYLAMDT Sbjct: 637 QLPIPALLATSAIHQHLVSKGLRTSTGLVVETGSARETHHFALLAGYGAEAIHPYLAMDT 696 Query: 541 LTEMAKGLPGDLSGEKAIYNFQKAIGKGLLKVMSKMGISTYMSYCGAQIFEAIGLNKALV 600 L +MAKGLPGDLS EKAIYNFQKA+GKGLLKVMSKMGISTYMSYCGAQIFEAIGL+KALV Sbjct: 697 LADMAKGLPGDLSPEKAIYNFQKAVGKGLLKVMSKMGISTYMSYCGAQIFEAIGLSKALV 756 Query: 601 DKYFKGTASNVEGIGVFEVAEEALRLHRAAFSSDPVLNNALDAGGEYAFRIRGEEHMWTP 660 DKYFKGTASNVEGIGVFEVAEEALRLH AAFS+DPVL NALDAGGEYAFRIRGEEHMWTP Sbjct: 757 DKYFKGTASNVEGIGVFEVAEEALRLHTAAFSADPVLANALDAGGEYAFRIRGEEHMWTP 816 Query: 661 DAIAKLQHSTRANNFNSYKEYAQIINDQSKRHMTLRGLFEFKIDPSKAISIDEVEPAKEI 720 DAIAKLQHSTR+N++N+YKEYAQIINDQSKRHMTLRGLFEFKIDPSKAISIDEVEPAKEI Sbjct: 817 DAIAKLQHSTRSNSYNTYKEYAQIINDQSKRHMTLRGLFEFKIDPSKAISIDEVEPAKEI 876 Query: 721 VKRFATGAMSLGSISTEAHATLAIAMNRIGGKSNTGEGGEDVNRYRNELKGIPIKQGATL 780 VKRFATGAMSLGSISTEAHATLAIAMNRIGGKSNTGEGGEDVNRYRNELKGIPIKQGATL Sbjct: 877 VKRFATGAMSLGSISTEAHATLAIAMNRIGGKSNTGEGGEDVNRYRNELKGIPIKQGATL 936 Query: 781 ASEIGKDVIEVDIPLQAGDSLRSRIKQVASGRFGVSAEYLISADQIQIKMAQGAKPGEGG 840 ASEIGK+ IEVDIPL GDSLRSRIKQVASGRFGVSAEYLISADQIQIKMAQGAKPGEGG Sbjct: 937 ASEIGKEHIEVDIPLLEGDSLRSRIKQVASGRFGVSAEYLISADQIQIKMAQGAKPGEGG 996 Query: 841 QLPGHKVTDYIATLRVSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPRASISVKLVS 900 QLPGHKV++YIA LRVSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPRASISVKLVS Sbjct: 997 QLPGHKVSEYIAKLRVSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPRASISVKLVS 1056 Query: 901 EVGVGTVAAGVAKAKSDHVVIAGHDGGTGASPLSSIKHAGSPWELGLAETQQTLVLNGLR 960 EVGVGTVAAGVAKAKSDHVVIAGHDGGTGASPLSSIKHAGSPWELGLAETQQTLVLNGLR Sbjct: 1057 EVGVGTVAAGVAKAKSDHVVIAGHDGGTGASPLSSIKHAGSPWELGLAETQQTLVLNGLR 1116 Query: 961 NRIRVQADGQMKTGRDVVIGALLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDP 1020 NRIRVQADGQMKTGRDVVIGA+LGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDP Sbjct: 1117 NRIRVQADGQMKTGRDVVIGAMLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDP 1176 Query: 1021 VLRAKFSGKPEHVVNFFFFIAEEARQIMAQLGIRTFNELIGRSDLLDRTKAIQHWKAQGL 1080 VLRAKFSGKPEHVVNFFFFIAEEARQIMAQLGIR F+ELIGR+DLLDR+KAI HWKA+GL Sbjct: 1177 VLRAKFSGKPEHVVNFFFFIAEEARQIMAQLGIRKFDELIGRADLLDRSKAIAHWKAKGL 1236 Query: 1081 DFSKIFYQPVSATPYFHTEEQDHGLDKALDHKLIAQAKTALEKGEKVSFISPIRNLNRTV 1140 DFS+IF+QP S P +HT+ QDHGLDKALDHKLIAQAKTALEKGEKVSFISPI+NLNRTV Sbjct: 1237 DFSRIFHQPESKLPVYHTDHQDHGLDKALDHKLIAQAKTALEKGEKVSFISPIKNLNRTV 1296 Query: 1141 GAMLSGEVAKRYGNEGLPDDTIHIQLQGTAGQSAGAFLAHGVTLDLVGEGNDYVGKGLSG 1200 GAMLSGEVAKRYG+EGLPDDTIHIQLQGTAGQSAGAFLA GVTLDLVGEGNDYVGKGLSG Sbjct: 1297 GAMLSGEVAKRYGDEGLPDDTIHIQLQGTAGQSAGAFLAKGVTLDLVGEGNDYVGKGLSG 1356 Query: 1201 GRIIVRPNTEFRGRAVDNMISGNTVLYGATTGEAFINGVVGERFAVRNSGAIAVVEGTGD 1260 GRIIVRPNTEFRGRAVDNMISGNTVLYGA +GEAFINGV GERFAVRNSGA AVVEGTGD Sbjct: 1357 GRIIVRPNTEFRGRAVDNMISGNTVLYGAISGEAFINGVAGERFAVRNSGATAVVEGTGD 1416 Query: 1261 HGCEYMTGGTVVVLGATGRNFAAGMSGGIAYVYDEAGDFAGKCNMAMVALDKVLSDTEQE 1320 HGCEYMTGGTVVVLG TGRNFAAGMSGGIAYVYD GDFAGKCN +MV+L+KVLSDTEQE Sbjct: 1417 HGCEYMTGGTVVVLGNTGRNFAAGMSGGIAYVYDPEGDFAGKCNTSMVSLEKVLSDTEQE 1476 Query: 1321 ASTDRAVWHSLQRGGDGQTDEVILRGLIERHFKYTGSTRARAMDDLLTRRSAXPARYLLD 1380 + R +WHSLQRGG+ QTDE ILRGLIERHFKYTGSTR ARYLLD Sbjct: 1477 QTLGRDIWHSLQRGGERQTDEAILRGLIERHFKYTGSTR---------------ARYLLD 1521 Query: 1381 NWAASRGKFVKVFPTEYKRALGELNAAKVAPKDKVPA 1417 NWAASR KFVKVFPTEYKRAL EL AA K+KV A Sbjct: 1522 NWAASRAKFVKVFPTEYKRALAELAAAAQTKKEKVAA 1558 >gi|300312905|ref|YP_003776997.1| glutamate synthase [Herbaspirillum seropedicae SmR1] Length = 531 Score = 132 bits (333), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 104/330 (31%), Positives = 144/330 (43%), Gaps = 56/330 (16%) Query: 709 ISIDEVEPAKEIVKRFATGAMSLGSISTEAHATLAIAMNRIGGKSNTGEGGEDVNRYRNE 768 + D +P V F AMS GS+S A L + +TGEG V+ Y E Sbjct: 142 VGADRAQPYSMSV--FNVSAMSFGSLSANAIRALNQGAKKGNFAHDTGEG--SVSEYHRE 197 Query: 769 LKGIPIKQGATLASEIGKDVIEVDIPLQAGDSLRSRIKQVASGRFGV-------SAEYLI 821 G + Q+ASG FG + E Sbjct: 198 FGG-------------------------------DLVWQIASGYFGCRNADGSFNDEKFT 226 Query: 822 SADQ------IQIKMAQGAKPGEGGQLPGHKVTDYIATLRVSVPGVGLISPPPHHDIYSI 875 + Q I++K++QGAKPG GG LP K+T IA R GV ISP H + Sbjct: 227 AQAQSPQIKMIEVKLSQGAKPGHGGVLPAAKITPEIAATRGIPMGVDCISPAVHSSFSTP 286 Query: 876 EDLAQLIHDLKNVNPRASISVKLVSEVGVGTVAAGVAKAK------SDHVVIAGHDGGTG 929 L + I L+ ++ + +KL VG G+AKA D + + G +GGTG Sbjct: 287 IGLLEFIEKLRGLSGGKPVGIKLC--VGHPWEFFGIAKAMLKTDIVPDFITVDGSEGGTG 344 Query: 930 ASPLSSIKHAGSPWELGLAETQQTLVLNGLRNRIRVQADGQMKTGRDVVIGALLGADEFG 989 A+PL + H G P + GL TLV GLR+++++ A G++ T DV LGAD Sbjct: 345 AAPLEFVDHVGMPLQEGLVMVHNTLVGIGLRDKVKIGASGKIITAFDVARTLALGADWCN 404 Query: 990 FATAPLVVEGCIMMRKCHLNTCPVGVATQD 1019 A + GCI + CH + CP GVATQ+ Sbjct: 405 SARGFMFALGCIQSQSCHTDRCPTGVATQN 434 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 4,401,490 Number of extensions: 191227 Number of successful extensions: 540 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 536 Number of HSP's successfully gapped: 2 Length of query: 1417 Length of database: 1,619,870 Length adjustment: 97 Effective length of query: 1320 Effective length of database: 1,160,575 Effective search space: 1531959000 Effective search space used: 1531959000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 137 (57.4 bits)