BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0023.p (206 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310391|ref|YP_003774483.1| 3-oxoacyl-[acyl-carrier-protein... 304 3e-84 gi|300313636|ref|YP_003777728.1| short-chain alcohol dehydrogena... 85 5e-18 gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herb... 76 2e-15 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 66 2e-12 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 64 1e-11 >gi|300310391|ref|YP_003774483.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 283 Score = 304 bits (779), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 149/206 (72%), Positives = 172/206 (83%), Gaps = 3/206 (1%) Query: 1 MMFSPMNKPVHDWTGKRIWLIGASSGIGAALARQALAMGAQVAVSALIXRRVESLQAVAG 60 ++F PMN+P+ D G+RIWLIGASSGIGA LARQALA GA+VA+SA RR E L+ VAG Sbjct: 24 VLFGPMNEPMADLRGRRIWLIGASSGIGAELARQALAAGARVALSA---RRAEVLEEVAG 80 Query: 61 TNGNALILPFDVLDHAAWRTRHAEVCAAFGGVDLMLFCAADYRPERSWEVSDDVAEQTIK 120 + A I P DVL H AWR +HA + AFGGVDL++FCAA YRPERSWEV +D AE TI+ Sbjct: 81 AHPEAFIAPLDVLCHDAWRDQHARIVEAFGGVDLLIFCAARYRPERSWEVQEDEAEHTIR 140 Query: 121 TNLVSVYSALSSVLPDMLAQGSGGIALVASVAGYMGLPNASVYGPGKAALINLAEILYSD 180 TNL SVYSAL + LPDMLA+GSGGIALVASVAGY+GLP A+VYGPGKAALINLAEILYSD Sbjct: 141 TNLASVYSALGAALPDMLARGSGGIALVASVAGYVGLPGATVYGPGKAALINLAEILYSD 200 Query: 181 LRSKGISVYLINPGFVKTGLTEKNDF 206 LR KG++VYL+NPGFVKT LT+KNDF Sbjct: 201 LRPKGVNVYLVNPGFVKTDLTDKNDF 226 >gi|300313636|ref|YP_003777728.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 268 Score = 84.7 bits (208), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 65/200 (32%), Positives = 100/200 (50%), Gaps = 14/200 (7%) Query: 17 RIWLIGASSGIGAALARQALAMGAQVAVSALIXRRVESLQAVAGTNGNA---LILPFDVL 73 R++L GASSG+G ALAR A +Q AV L+ RR L+A+ +A + DV Sbjct: 12 RVFLTGASSGLGMALAR---AYASQGAVLGLVARRTAELEALRAALPHAERHRVYALDVT 68 Query: 74 DHAAWRTRHAEVCAAFGGVDLMLFCAADYRPERSWEVSDDVA--EQTIKTNLVSVYSALS 131 DHAA FGG D+++ A + + E +D+A E+ + N+ + ++ + Sbjct: 69 DHAALAAAATSFMDEFGGADVVIANAGISQGTLT-EFQEDLAAFERILAVNVTATFATFT 127 Query: 132 SVLPDMLAQGSGG-----IALVASVAGYMGLPNASVYGPGKAALINLAEILYSDLRSKGI 186 + M A + + SVAG GLP A Y K+A+I+ E L +LR+ GI Sbjct: 128 PFVARMKASPEAQRRRCRLVGIGSVAGIRGLPGAGAYSASKSAVISYCESLRVELRASGI 187 Query: 187 SVYLINPGFVKTGLTEKNDF 206 V I PG++ T +T N + Sbjct: 188 KVVTIAPGYIDTPMTRVNTY 207 >gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 76.3 bits (186), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 53/188 (28%), Positives = 92/188 (48%), Gaps = 7/188 (3%) Query: 15 GKRIWLIGASSGIGAALARQALAMGAQVAVSALIXRRVESLQAVAGTNGNALI----LPF 70 GKR+ + G SGIGAAL + GAQV + E QA+ + +A I P Sbjct: 19 GKRVVITGGGSGIGAALVEAFVGQGAQVC---FLDIAAEPSQALVASLKDAPIAPRFFPC 75 Query: 71 DVLDHAAWRTRHAEVCAAFGGVDLMLFCAADYRPERSWEVSDDVAEQTIKTNLVSVYSAL 130 ++++ A R E+ GGVD+++ AA+ ++ +V+ ++ + NL + Sbjct: 76 NLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYWDERLAVNLRHQFFCA 135 Query: 131 SSVLPDMLAQGSGGIALVASVAGYMGLPNASVYGPGKAALINLAEILYSDLRSKGISVYL 190 +VLP M + G I S++ ++GLP+ ++Y KA + + + D G+ V Sbjct: 136 QAVLPGMRERKQGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMARDFGRDGVRVNA 195 Query: 191 INPGFVKT 198 I PG ++T Sbjct: 196 IIPGAIRT 203 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 65.9 bits (159), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 60/190 (31%), Positives = 90/190 (47%), Gaps = 9/190 (4%) Query: 15 GKRIWLIGASSGIGAALARQALAMGAQVAVSALIXRRVESLQAVAG----TNGNALILPF 70 GK + GAS+GIG A A GA + ++A RR ++L+ VA G A ++ Sbjct: 6 GKVALITGASAGIGRATALLFARHGAALILTA---RRQDALEQVAALICAQGGRASVVAG 62 Query: 71 DVLDHAAWRTRHAEVCAAFGGVDLMLFCAADYRPERSW-EVSDDVAEQTIKTNLVSVYSA 129 DV + A A FGG+D+ + A + E+S + T+ NL S + Sbjct: 63 DVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLG 122 Query: 130 LSSVLPDMLAQGSGGIALVASVAGY-MGLPNASVYGPGKAALINLAEILYSDLRSKGISV 188 +P MLA+G+G I +S G GLP + YG KA L+ L + + +D +GI Sbjct: 123 ARCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRA 182 Query: 189 YLINPGFVKT 198 + PG V T Sbjct: 183 NALLPGGVDT 192 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 63.5 bits (153), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 59/191 (30%), Positives = 91/191 (47%), Gaps = 8/191 (4%) Query: 13 WTGKRIWLIGASSGIGAALARQALAMGAQVAVSALIXRRVESL-QAVAGTNGNALILPFD 71 + K + + G S GIG A A+ A GA+V VS RR + L QAV A+ +P D Sbjct: 7 FEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSG---RRADKLEQAVREIGNGAVGVPGD 63 Query: 72 VLDHAAWRTRHAEVCAAFGGVDLMLFCAADYRPERS--WEVSDDVAEQTIKTNLVSVYSA 129 +A V A G +D++ A E + ++ + N+ + + Sbjct: 64 AAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLFS 123 Query: 130 LSSVLPDMLAQGSGGIALVASVAGYMGLPNASVYGPGKAALINLAEILYSDLRSKGISVY 189 + LP +++QG G I L SVAG G P S+Y KAA+ + A +DL+ +GI V Sbjct: 124 VQKALP-LMSQG-GAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKERGIRVN 181 Query: 190 LINPGFVKTGL 200 ++ PG +T L Sbjct: 182 VVAPGGTETRL 192 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 555,430 Number of extensions: 19132 Number of successful extensions: 123 Number of sequences better than 1.0e-08: 31 Number of HSP's gapped: 111 Number of HSP's successfully gapped: 31 Length of query: 206 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 125 Effective length of database: 1,236,335 Effective search space: 154541875 Effective search space used: 154541875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)