BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0014.p (770 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 481 e-136 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 398 e-112 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 362 e-101 gi|300311453|ref|YP_003775545.1| signal transduction containing ... 314 1e-86 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 310 3e-85 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 481 bits (1238), Expect = e-136, Method: Compositional matrix adjust. Identities = 248/555 (44%), Positives = 350/555 (63%), Gaps = 8/555 (1%) Query: 221 EKSLRVAATVFQSQEAMWVMGPDRRILDVNHAFVKLTGFNLAELMDKATLPFYLEQGEHD 280 E LR++A F S+E M + + +IL VN AF ++TG++ E++ K P L H Sbjct: 471 EAELRISAVAFDSREGMLITDANSKILRVNKAFTEITGYSAEEIVGKH--PNLLRSDRHG 528 Query: 281 --YRESMWNAVKETGQWDGEVQAKKKNNERYMARLTMTAVRDATGKITHYVGTQTDITQQ 338 + + M +++ G+W GE+ ++KN E Y LT++AV++ ++THYV T DI+ + Sbjct: 529 PAFFQEMRESIRRFGKWQGEIWDRRKNGEVYPEWLTISAVKNKDQQVTHYVSTHHDISDR 588 Query: 339 KLLLAETVRLADYDSLTELPNRRLLIERVESAIAANADNGKSFALLVIDIDNFKDLNDSL 398 KL LA +D+LT LPNR LL++R++ AIA + ALL ID+D+FK LND++ Sbjct: 589 KLAEERIRELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAALLFIDLDHFKTLNDTI 648 Query: 399 GHNIGDQLLKKIANRLIAAADSEENVARLGGDEFVVLLQGIDTTDSEIAV--RRFAQRVL 456 GH GD LLK++A RL+A + VAR+GGDEFVV+L+G++ E A R +++L Sbjct: 649 GHEKGDTLLKQVAQRLLANVRENDTVARVGGDEFVVVLEGLNELQQEAATQTRAIGEKIL 708 Query: 457 TAMSEPFELAGLSHHATCCIGVALMQGSDLTAQDMLRRGDLAMYQAKKNGRNTFCLFSAE 516 TA+ ++L + + T IG + G + +++++ DLAMY+AK+ GRN F Sbjct: 709 TALGSTYQLDDVEYRTTASIGATVFLGRAASVDELMKQADLAMYKAKETGRNALRFFDPA 768 Query: 517 IENAVNFRTALEFDLRKAIDRQEFYLHXKVDL--GGRVIGVEALLRWQNPERGSVAPGIF 574 ++ AV R LE LRKAID + LH + + GGRV G E L+RWQ+P+RG V PG F Sbjct: 769 MQTAVLERATLEAGLRKAIDGNQLLLHYQPQVVEGGRVTGAEVLVRWQHPQRGMVPPGDF 828 Query: 575 VPVAEAAGLMFAIGQWVLRQACEQSVRWQLSRPDHPLVVAVNVSAIQFGHSEFTTDVSEI 634 +P+AE GL+ ++G WVL AC Q W L +AVNVSA Q +F V + Sbjct: 829 IPLAEETGLILSLGNWVLETACRQLATWAAQPQMADLSIAVNVSAPQLREPDFVQTVLDT 888 Query: 635 ISRTGVNPALLKMELTETMMVDDVQETVEKMKILKGLGISLSLDDFGTGYSSLSLLKRLP 694 I RTG NP LK+ELTE+++VD+V++ ++KM LK G+ SLDDFGTGYSSLS LKRLP Sbjct: 889 IERTGANPRRLKLELTESLLVDNVEDIIQKMFALKAYGVEFSLDDFGTGYSSLSYLKRLP 948 Query: 695 LDQLKIDQSFVRDILVDASDVAIAKSIIALGSALNVDVIAEGVETLEVRDFLASIGCLKY 754 L+QLKIDQSFVRD+L+D +D +IAK+I+ L +L + VIAEGVET R FLA GC Y Sbjct: 949 LNQLKIDQSFVRDVLIDPNDASIAKTIVNLAQSLGLGVIAEGVETDAQRSFLADAGCHAY 1008 Query: 755 QGYAFSRPGPAEQLQ 769 QGY F RP E + Sbjct: 1009 QGYFFCRPVTVEAFE 1023 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 398 bits (1022), Expect = e-112, Method: Compositional matrix adjust. Identities = 242/616 (39%), Positives = 352/616 (57%), Gaps = 44/616 (7%) Query: 189 LVATVGLFLCALGVAAYLR----------REVEIRTRELRNS--------------EKSL 224 L+ VG+ LG AAY+R + VE RT ELR + E++L Sbjct: 332 LILLVGVIASLLG-AAYMRALSMRSHRIQQLVEERTAELRTTNALLSDDIKARRLAERAL 390 Query: 225 RVAATVFQ-SQEAMWVMG---PDRRILDVNHAFVKLTGFNLAELMDKATLPFYLEQGEHD 280 R+ + S A+ ++ PD I VN AF ++TG++ E + K+ +LE + D Sbjct: 391 RLRHQAIEASANAILILSAQEPDYTIEYVNPAFARITGYSSTEAVGKSIR--FLEGRDSD 448 Query: 281 YR--ESMWNAVKETGQWDGEVQAKKKNNERYMARLTMTAVRDATGKITHYVGTQTDITQQ 338 E++W A++E + + ++ K+ + VRD +G++TH+V DIT Sbjct: 449 PEGFETVWIAMREQREHNVVLRNYHKDGSELWNDFYIAPVRDESGEVTHFVAALYDITSM 508 Query: 339 KLLLAETVRLADYDSLTELPNRRLLIERVESAIAANADNGKSFALLVIDIDNFKDLNDSL 398 K AE A D+LT L NR +L +E AI ++ ++ +D+D FK +ND+L Sbjct: 509 KRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDTL 568 Query: 399 GHNIGDQLLKKIANRLIAAADSEENVARLGGDEFVVLLQGID--TTDSEIAVRRFAQRVL 456 GH GD LL+ +A RL AA + VARLGGDEF+++L D T D+ R QR++ Sbjct: 569 GHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILPEQDELTLDT-----RSLQRIM 623 Query: 457 TAMSEPFELAGLSHHATCCIGVALMQGSDLTAQDMLRRGDLAMYQAKKNGRNTFCLFSAE 516 A++ P L G + TC +GVA + +++ D+AMY+ K+ GRN F ++ Sbjct: 624 DAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPA 683 Query: 517 IENAVNFRTALEFDLRKAIDRQEFYLHX--KVDLG-GRVIGVEALLRWQNPERGSVAPGI 573 + R +E DLR A+D EF LH KVDL GRVIG EAL+RWQ+P+ G V+P Sbjct: 684 MNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPAR 743 Query: 574 FVPVAEAAGLMFAIGQWVLRQACEQSVRWQLSRPDHPLVVAVNVSAIQFGHSEFTTDVSE 633 F+ +AE GL+ IG WVLR AC Q +WQL + V+VN+S QF + + +++ Sbjct: 744 FIGLAEETGLILPIGAWVLRAACTQCRQWQLGGYAD-ISVSVNLSVGQFLQKDLVSSIAD 802 Query: 634 IISRTGVNPALLKMELTETMMVDDVQETVEKMKILKGLGISLSLDDFGTGYSSLSLLKRL 693 ++ TG+ P L++ELTE++M+ DV+ + + LK LG+ LS+DDFGTGYSSL+ LKR Sbjct: 803 VLEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRF 862 Query: 694 PLDQLKIDQSFVRDILVDASDVAIAKSIIALGSALNVDVIAEGVETLEVRDFLASIGCLK 753 P+D LKID+SFVRDI +DA D AI SII+L L + VIAEGVET E D+L GC + Sbjct: 863 PIDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDE 922 Query: 754 YQGYAFSRPGPAEQLQ 769 QGY FS P PA++ + Sbjct: 923 IQGYLFSPPVPADKFE 938 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 362 bits (930), Expect = e-101, Method: Compositional matrix adjust. Identities = 211/555 (38%), Positives = 325/555 (58%), Gaps = 19/555 (3%) Query: 217 LRNSEKSLRVAATVFQSQ-EAMWVMGPDRRILDVNHAFVKLTGFNLAE----LMDKATLP 271 L+ +E+ R ++F++ E ++ PD + VN A ++ G++ E + + Sbjct: 134 LQEAERRYR---SIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDLIVGLRDISHQ 190 Query: 272 FYLEQGEHDYRESMWNAVKETGQWDG-EVQAKKKNNERYMARLTMTAVRDATGKITHYVG 330 Y+E + R+ ++E G E + +++ E AV D GK++ Y G Sbjct: 191 LYVEP---ERRQEFMRLMEEQGSVSNFESRVYRRDGEIIWISENARAVYDEAGKLSCYEG 247 Query: 331 TQTDITQQKLLLAETVRLADYDSLTELPNRRLLIERVESAIAANADNGKSFALLVIDIDN 390 T IT++K AE A +D+LT LPNR +L E ++ A+ G A+ +D+D Sbjct: 248 TVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAFVDLDQ 307 Query: 391 FKDLNDSLGHNIGDQLLKKIANRLIAAADSEENVARLGGDEFVVLLQGIDTTDSEIAVRR 450 FK +NDSLGH +GD+LLK +A RL A + VAR GGDEFV++LQ + T+ E + Sbjct: 308 FKFINDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQ--NQTEGEAGIAE 365 Query: 451 FAQRVLTAMSEPFELAGLSHHATCCIGVALMQGSDLTAQDMLRRGDLAMYQAKKNGRNTF 510 QR+L A++ P++ T IGV+ + +L+ D AMY+AK+ GRN F Sbjct: 366 VMQRILAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNF 425 Query: 511 CLFSAEIENAVNFRTALEFDLRKAIDRQEFYLHXKVDLG---GRVIGVEALLRWQNPERG 567 F+ +++ V+ R + +LR+A+D+ EF L+ + L GR+IG EAL+RW++P++G Sbjct: 426 QFFAPWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQG 485 Query: 568 SVAPGIFVPVAEAAGLMFAIGQWVLRQACEQSVRWQLSRPDHPLVVAVNVSAIQFGHSEF 627 V P F+P AE AGL+ IG+WVLR AC Q+ WQ + P+ VAVN+S Q S Sbjct: 486 MVPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLP-PIPVAVNLSPRQLNQS-L 543 Query: 628 TTDVSEIISRTGVNPALLKMELTETMMVDDVQETVEKMKILKGLGISLSLDDFGTGYSSL 687 V+ +++++G+ + L++E+TE +++ D +++V + LK LG+ +S+DDFGTGYSSL Sbjct: 544 PDFVAGVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSL 603 Query: 688 SLLKRLPLDQLKIDQSFVRDILVDASDVAIAKSIIALGSALNVDVIAEGVETLEVRDFLA 747 S L+R P+D LKID+SFVRDI+ DA AI K+II+L LN+ VIAEGVE E FL Sbjct: 604 SYLRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLK 663 Query: 748 SIGCLKYQGYAFSRP 762 C + QGY F +P Sbjct: 664 ENACDEVQGYYFGKP 678 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 314 bits (805), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 214/567 (37%), Positives = 305/567 (53%), Gaps = 18/567 (3%) Query: 207 RREVEIRTRELRNSEKSLRVAATVF-QSQEAMWVMGPDRRILDVNHAFVKLTGFNLAELM 265 R + E R R L E L A + Q E++ M P I N +L G++ E + Sbjct: 104 RADTEQRVRALDQIEAKLEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSAMEAI 163 Query: 266 DKATLPFYLEQGEHDYRESMWNAVKETGQWDGEVQAKKKNNERYMARLTMTAVRDATGKI 325 + L Y + E D S+ + E G + EV+ +KK+ E + A L+++ +RD G Sbjct: 164 GRNVLFLYENEDEED---SLHDLFLEHGGREMEVRRRKKSGEVFWASLSLSVMRDQNGHP 220 Query: 326 THYVGTQTDITQQKLLLAETVRLADYDSLTELPNRRLLIERVESAIAANADNGKSFALLV 385 +G DIT++K LA YD+LT LPNR LL V+ A+ ++ Sbjct: 221 IGLIGYLNDITERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMF 280 Query: 386 IDIDNFKDLNDSLGHNIGDQLLKKIANRLIAAADSEENVARLGGDEFVVLLQGIDTTDSE 445 ID++ FK +ND+LGH GD LL ++A RL A ++ VARLG DEF V L GID DS Sbjct: 281 IDLNRFKPINDTLGHVAGDMLLVEVAMRLRRALREQDVVARLGADEFAVALFGIDK-DSP 339 Query: 446 IAVRRFAQRVLTAMSEPFELAGLSHHATCCIGVALMQGSDLTAQDMLRRGDLAMYQAKKN 505 V AQ++L +PF + G IGV+L + + +LR D+AMY+AK+ Sbjct: 340 AYV---AQKLLALFDDPFFIDGHELRVGASIGVSLYPENATDTETLLRLADIAMYRAKQG 396 Query: 506 GRNT---FCLFSAEIENAVNFRTALEFDLRKAIDRQEF--YLHXKVDLGGRVI-GVEALL 559 G N + +S E+ +E L A + E Y KVD+ I G EAL+ Sbjct: 397 GENNEGGYACYSEEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALV 456 Query: 560 RWQNPERGSVAPGIFVPVAEAAGLMFAIGQWVLRQACEQSVRWQ-LSRPDHPLVVAVNVS 618 RWQ+PERG + PG F+P+AE GL+ + WVL C Q+ W+ P P+ +A+NV+ Sbjct: 457 RWQHPERGLLLPGEFIPIAEETGLIVQLSDWVLDAVCRQARAWKDAGLP--PIRIALNVT 514 Query: 619 AIQFGHSEFTTDVSEIISRTGVNPALLKMELTETMMVDDVQETVEKMKILKGLGISLSLD 678 A +F S V + R ++ L++E+TE+M++ + M+ + GLGI++SLD Sbjct: 515 AREFTRS-LPDRVGAALERHMLSGEWLELEITESMLMHSTDRVISIMEKICGLGITVSLD 573 Query: 679 DFGTGYSSLSLLKRLPLDQLKIDQSFVRDILVDASDVAIAKSIIALGSALNVDVIAEGVE 738 DFGTGYSSLS LKR P++ LKID+SF I D +D AIA +II + L VIAEGVE Sbjct: 574 DFGTGYSSLSYLKRFPINTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVE 633 Query: 739 TLEVRDFLASIGCLKYQGYAFSRPGPA 765 + +FL GC + QGY FSRP PA Sbjct: 634 DEKQFNFLREAGCNELQGYIFSRPVPA 660 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 310 bits (793), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 170/427 (39%), Positives = 259/427 (60%), Gaps = 10/427 (2%) Query: 344 ETVRLADYDSLTELPNRRLLIERVESAIAANADNGKSFALLVIDIDNFKDLNDSLGHNIG 403 E ++ +D LT LPNR LL +R+ AI A FAL+ +D+D FK +NDSLGH++G Sbjct: 261 ELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAINDSLGHHVG 320 Query: 404 DQLLKKIANRLIAAADSEENVARLGGDEFVVLLQGIDTTDSEIAVRRFAQRVLTAMSEPF 463 D+LL ++A RL +++ + + VARLGGDEFV+L+ ++T + + V A++++ A+++PF Sbjct: 321 DRLLVEVAQRLTSSSRANDTVARLGGDEFVILVD-LNTPEDAMGV---AEKLVEAVNQPF 376 Query: 464 ELAGLSHHATCCIGVALMQGSDLTAQDMLRRGDLAMYQAKKNGRNTFCLFSAEIENAVNF 523 ++ + +G+A+ T D++ D AMY K++GRN + F + Sbjct: 377 KVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMNANAQQ 436 Query: 524 RTALEFDLRKAIDRQEFYL--HXKVDL-GGRVIGVEALLRWQNPERGSVAPGIFVPVAEA 580 + DLR A++R +F L K L G V+G EALLRW++P G VAP F+ +AE Sbjct: 437 QLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIGLAER 496 Query: 581 AGLMFAIGQWVLRQACEQSVRW-QLSRPDHPLVVAVNVSAIQFGHSEFTTDVSEIISRTG 639 +GL+ IG WVL +AC Q W L D ++VN+SA+QF V + R Sbjct: 497 SGLILPIGAWVLDEACRQMREWFALGYVD--WTMSVNLSALQFADPHLLEVVRTTLERHQ 554 Query: 640 VNPALLKMELTETMMVDDVQETVEKMKILKGLGISLSLDDFGTGYSSLSLLKRLPLDQLK 699 + P L +E+TE+ + D + +++ ++ + LG+ +S+DDFGTGYSSL LKRLP ++LK Sbjct: 555 LPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANELK 614 Query: 700 IDQSFVRDILVDASDVAIAKSIIALGSALNVDVIAEGVETLEVRDFLASIGCLKYQGYAF 759 ID+ FVR++ D D AI +I+ALG LN+ ++AEGVET+E +DFL + GC QGY Sbjct: 615 IDRGFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQGYLM 674 Query: 760 SRPGPAE 766 RP E Sbjct: 675 GRPMTPE 681 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,286,024 Number of extensions: 94834 Number of successful extensions: 424 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 338 Number of HSP's successfully gapped: 44 Length of query: 770 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 677 Effective length of database: 1,179,515 Effective search space: 798531655 Effective search space used: 798531655 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)