BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0014.p (125 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 164 3e-42 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 65 1e-12 gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogena... 60 6e-11 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 59 2e-10 gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogena... 57 6e-10 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 164 bits (414), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 84/96 (87%), Positives = 90/96 (93%) Query: 9 SLSNKVAFVQGGSRGIGAAIVKRLVQEGAIVAFTYVSSPDKAQALVSEVEAKGGRALAIQ 68 +L +KVAFVQGGSRGIGAAIV+RL EGA VAFTYVSSPDKAQALVS V+AKGGRALAIQ Sbjct: 14 TLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQ 73 Query: 69 ADSSDTAALQQAIRIAAETFGRLDILVNNAGVLAMG 104 ADS+D AALQQAIR+AAETFGRLDILVNNAGVLAMG Sbjct: 74 ADSADAAALQQAIRLAAETFGRLDILVNNAGVLAMG 109 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 65.5 bits (158), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 39/94 (41%), Positives = 56/94 (59%) Query: 8 LSLSNKVAFVQGGSRGIGAAIVKRLVQEGAIVAFTYVSSPDKAQALVSEVEAKGGRALAI 67 +++++KV V GGSRGIGAA Q G VA YV++ A A+V++++A GGRA+A+ Sbjct: 1 MNMADKVILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAV 60 Query: 68 QADSSDTAALQQAIRIAAETFGRLDILVNNAGVL 101 D + + Q GR+ LVNNAGVL Sbjct: 61 GGDVAREEDVLQLFATVDGELGRVTALVNNAGVL 94 >gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 59.7 bits (143), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 40/94 (42%), Positives = 52/94 (55%), Gaps = 2/94 (2%) Query: 7 NLSLSNKVAFVQGGSRGIGAAIVKRLVQEGAIVAFTYVSSPDKAQALVSEVEAKGGRALA 66 +L+L +KV V GG+ GIGAAI +L EGAI S+P+ + A RA Sbjct: 2 DLNLQDKVVIVTGGASGIGAAISLQLAAEGAIPVVFARSAPES--QFWERLTAVQPRAAL 59 Query: 67 IQADSSDTAALQQAIRIAAETFGRLDILVNNAGV 100 Q + D A ++A+ E FGRLD LVNNAGV Sbjct: 60 FQLELQDDARCREAVAGTIERFGRLDGLVNNAGV 93 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 58.5 bits (140), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 31/89 (34%), Positives = 47/89 (52%) Query: 10 LSNKVAFVQGGSRGIGAAIVKRLVQEGAIVAFTYVSSPDKAQALVSEVEAKGGRALAIQA 69 L+ KVA + GG GIG A+ +EGA +A Y+ + A+ +V+A L + Sbjct: 123 LAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPG 182 Query: 70 DSSDTAALQQAIRIAAETFGRLDILVNNA 98 D + A+R + +GRLD+LVNNA Sbjct: 183 DVGSPEFCRSAVRAVMDAYGRLDVLVNNA 211 >gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 56.6 bits (135), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 37/96 (38%), Positives = 56/96 (58%), Gaps = 6/96 (6%) Query: 11 SNKVAFVQGGSRGIGAAIVKRLVQEGAIVAFTYVSSPDKAQALVSEVEAKGGRALAIQAD 70 S ++A + GGSRG+G L ++GA V TYV + A+A V+E++A G A A++ D Sbjct: 7 SPRIALITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQALGAVAAALRLD 66 Query: 71 SSDTA---ALQQAIRIAAETFGR---LDILVNNAGV 100 +D+A A QA+R A +T + LVNN G+ Sbjct: 67 LADSASFPAFAQAVREALKTHWQAEHFHYLVNNGGM 102 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.129 0.345 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 376,404 Number of extensions: 14157 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 18 Length of query: 125 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 50 Effective length of database: 1,264,745 Effective search space: 63237250 Effective search space used: 63237250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)