BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0011.p (475 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 670 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 373 e-104 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 351 7e-98 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 244 8e-66 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 124 2e-29 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 670 bits (1728), Expect = 0.0, Method: Compositional matrix adjust. Identities = 345/474 (72%), Positives = 412/474 (86%), Gaps = 4/474 (0%) Query: 6 TPSRAHWLPFIVAATFFMEYLDTTIIATALPQMAQAFKVGPNELSLGMTAYMLTLAVFIP 65 T +R LP+IVAATFFMEYLDTTIIATALPQMAQ+F VGPNELSLGMTAYMLTLAVFIP Sbjct: 25 TDARVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIP 84 Query: 66 ISGWIADRLGSRTVFASAIVIFTLASVLCGISGSVFEFTAARILQGMGGALMVPVGRLIV 125 ISGW+ADR GSRTVF AI IFTLAS+LCG S +V+EFTAAR+LQG+GGA+MVPVGR+IV Sbjct: 85 ISGWVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIV 144 Query: 126 VRNTSKDKLMKAISTITWPAIVAPVVGPSLGGFITTYFSWHWIFLINVPFGIAALTAVLR 185 VR+T KD+LMKAI+TITWPAIVAPVVGPS+GGFITTY SWHWIFL+NVPFG+AA TAV+ Sbjct: 145 VRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMM 204 Query: 186 IVPNERGNQRKPLDMIGFFLSGTALTSLLYGTELASHTDTNLLVAMLFIAGGLVLGVFSY 245 +V NE G ++PLD++GF LSG ALTSLLYGTE+ASH ++N+ VA+ F+A G+VLG ++ Sbjct: 205 VVRNEHGVSKRPLDLVGFLLSGAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAW 264 Query: 246 RHGMRREHPLLDYSTLKVSTFNVTVVAGSFTRIGIEAVPYLLPLLFQIGFGLSAFHSGLL 305 RH R EHPLLD+S L++ T++VTV+ GS TRIG++AVPYL+PL+FQIGFGL+AF SGLL Sbjct: 265 RHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLL 324 Query: 306 LLASALGNLGMKMFTTRVLQRFGFRRVALNNAVIAGVFVVACGLLTATTPVPVVLMTIFI 365 LLA+ALGNLGMK FTTR+LQRFGFR VA+NNA IAG ++ACGLLT TP+PV+L IF Sbjct: 325 LLATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFC 384 Query: 366 YGLSRSMQFTTLATLAYADVSEAQKSAASTLWSVAQQMTIGMGIAFGALALRVASYFNG- 424 YGLSRSMQF+TLATLAYAD+S+A+KS+ASTLWSVAQQMTIGMGIAFGALALR+A+ G Sbjct: 385 YGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGE 444 Query: 425 ---SGEAHFSVIDFHWAFAMAGLLTLCSALGYRHLAKDAGNNLGGGVRGAPKNR 475 + AHF++ DF WAFAMAG++TL S +GYR LA+DAG+N+GGG+R AP R Sbjct: 445 AIDASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAGSNIGGGMRAAPGKR 498 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 373 bits (958), Expect = e-104, Method: Compositional matrix adjust. Identities = 197/447 (44%), Positives = 278/447 (62%), Gaps = 2/447 (0%) Query: 26 LDTTIIATALPQMAQAFKVGPNELSLGMTAYMLTLAVFIPISGWIADRLGSRTVFASAIV 85 +D+T+I+T+LP +A V P L L +T+Y+++LAVFIPISGW+ADR GS T+F SAI Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 86 IFTLASVLCGISGSVFEFTAARILQGMGGALMVPVGRLIVVRNTSKDKLMKAISTITWPA 145 +F L S+LCG+S ++ +F AAR LQG+GGA+MVPVGRL+++R+ K L+KA+S +T PA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 146 IVAPVVGPSLGGFITTYFSWHWIFLINVPFGIAALTAVLRIVPNERGNQRKPLDMIGFFL 205 + PV+GP LGGFITTYF W WIF IN+P I L R +P+ + Q PLD+ GF L Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 206 SGTALTSLLYGTELASHTDTNLLVAMLFIAGGLVLGVFSYRHGMRREHPLLDYSTLKVST 265 S + L+ G A + VA+ + GLV H R PL+D + LK+ T Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 266 FNVTVVAGSFTRIGIEAVPYLLPLLFQIGFGLSAFHSGLLLLASALGNLGMKMFTTRVLQ 325 V GSF R+GI A+P+LLPL+ Q+GFG + FHSG+L ASA G + MK R L+ Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 300 Query: 326 RFGFRRVALNNAVIAGVFVVACGLLTATTPVPVVLMTIFIYGLSRSMQFTTLATLAYADV 385 R+GFRRV L N + + + A T TP ++++ + I G RS+QFT+L ++YADV Sbjct: 301 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 360 Query: 386 SEAQKSAASTLWSVAQQMTIGMGIAFGALALRVASYFNGSGEAHFSVIDFHWAFAMAGLL 445 + + S A+++ SVAQQ++IGMG+ AL++++ G A DF AF + G + Sbjct: 361 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQ--GHATLVAQDFWPAFLVVGAV 418 Query: 446 TLCSALGYRHLAKDAGNNLGGGVRGAP 472 C+ L R L DAG + G P Sbjct: 419 AACALLMLRQLPHDAGAEMAGRDDNRP 445 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 351 bits (901), Expect = 7e-98, Method: Compositional matrix adjust. Identities = 189/446 (42%), Positives = 282/446 (63%), Gaps = 10/446 (2%) Query: 26 LDTTIIATALPQMAQAFKVGPNELSLGMTAYMLTLAVFIPISGWIADRLGSRTVFASAIV 85 +D T++AT+LP +A+ P L L MTAY+ L VFIPISGW+AD+LG+R VF++A++ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 86 IFTLASVLCGISGSVFEFTAARILQGMGGALMVPVGRLIVVRNTSKDKLMKAISTITWPA 145 +F S+LC +SGS+ F AAR LQG+GGA+MVPVGR+I+ R+ K +L+KA+S +T PA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 146 IVAPVVGPSLGGFITTYFSWHWIFLINVPFGIAALTAVLRIVPNERGNQRKPLDMIGFFL 205 ++ PVVGP LGGFITTYF W W+FLIN+PF L R + N + + PLD+ GF L Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 206 SGTALTSLLYGTEL-ASH-TDTNLLVAMLFIAGGLVLGVFSYRHGMRREHPLLDYSTLKV 263 + ++ G L SH D VAM I G + V YRH + E PLLD+ L++ Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSI--GAIALVMYYRHSTQIEFPLLDFRLLRI 238 Query: 264 STFNVTVVAGSFTRIGIEAVPYLLPLLFQIGFGLSAFHSGLLLLASALGNLGMKMFTTRV 323 T + +VV GS RIG+ AVP+LLPL Q G G++AF +G + ASA G+L +++ RV Sbjct: 239 PTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRV 298 Query: 324 LQRFGFRRVALNNAVIAGVFVVACGLLTATTPVPVVLMTIFIYGLSRSMQFTTLATLAYA 383 L+R+GFR V + NAV AG+ + A G+ + TP +++ + + G+ ++QFT L ++AYA Sbjct: 299 LRRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYA 358 Query: 384 DVSEAQKSAASTLWSVAQQMTIGMGIAFGALALRVASYFNGSGEAHFSVI--DFHWAFAM 441 D+ ++L SV QQ+++G+G+ + L+++++ G H +++ DF AF + Sbjct: 359 DIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQG----HATIVEADFWPAFLV 414 Query: 442 AGLLTLCSALGYRHLAKDAGNNLGGG 467 GL ++ S L +AG L G Sbjct: 415 IGLFSVASVPISGKLPHNAGLELSRG 440 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 244 bits (624), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 154/455 (33%), Positives = 250/455 (54%), Gaps = 11/455 (2%) Query: 5 QTPSRAHWLPFIVAATFFMEYLDTTIIATALPQMAQAFKVGPNELSLGMTAYMLTLAVFI 64 P L +IVA+ FFM+ LDTTI+ TALP MA++ + + AY LT+A Sbjct: 46 NDPVTQRALLWIVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLT 105 Query: 65 PISGWIADRLGSRTVFASAIVIFTLASVLCGISGSVFEFTAARILQGMGGALMVPVGRLI 124 P SGW+ADR G+R ++ +AI++F+L S+LC ++ S+ AAR++QG+GG++++PVGRL Sbjct: 106 PASGWLADRFGTRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLA 165 Query: 125 VVRNTSKDKLMKAISTITWPAIVAPVVGPSLGGFITTYFSWHWIFLINVPFGIAALTAVL 184 V+R + + A+++I+ + PVVGP LGG++ SWHWIFLIN+P G+ + AVL Sbjct: 166 VLRAVPGAQYIAALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVL 225 Query: 185 RIVPNERGNQRKPLDMIGFFLSGTALT--SLLYGTELASHTDTNLLVAMLFIAGGLVLGV 242 +P++ D++G L + SL SH T +A+ +A VLG Sbjct: 226 YFLPDDSLAGTPRFDLLGCVLLSACMVAFSLALDVPFDSH-RTGWSIALFVVAALTVLGY 284 Query: 243 FSYRHGMRREHPLLDYSTLKVSTFNVTVVAGSFTRIGIEAVPYLLPLLFQIGFGLSAFHS 302 +RH PL F++ + RIG AVP+LLPLL Q+ G S H+ Sbjct: 285 --WRHASVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHA 342 Query: 303 GLLLLASALGNLGMKMFTTRVLQRFGFRRVALNNAVIAGVFVVACGLLTATTPVPVVLMT 362 G++LL +A+ K + +++RFG+ R L N I G +++ L+T P+ ++ Sbjct: 343 GMVLLPAAIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQ 402 Query: 363 IFIYGLSRSMQFTTLATLAYADVSEAQKSAASTLWSVAQQMTIGMGIAFGALALRV-ASY 421 + I+G S SMQF + ++ + + + ++L+S+ Q + IG+G+ G + + S Sbjct: 403 LAIFGASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSE 462 Query: 422 FNGSGEAHFSVIDFHWAFAMAGLLTLCSALGYRHL 456 + A F + FA+ G +TL SA +R + Sbjct: 463 VGTATNASFQI-----TFAIVGAITLASAWVFRKM 492 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 124 bits (311), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 84/273 (30%), Positives = 144/273 (52%), Gaps = 18/273 (6%) Query: 1 MTAAQTPSRAHWLP------------FIVAATFFMEYLDTTIIATALPQMAQAFKVGPNE 48 M + TPS+A+ P V+ FM LD++I A+P ++ V +E Sbjct: 1 MATSPTPSQAYTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE 60 Query: 49 LSLGMTAYMLTLAVFIPISGWIADRLGSRTVFASAIVIFTLASVLCGISGSVFEFTAARI 108 + +T++ A+ IP++GW+ RLG+ +F +++++F LAS LCG++ ++ AAR+ Sbjct: 61 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARV 120 Query: 109 LQGMGGALMVPVGRLIVVRNTSKDKLMKAISTITWPAIVAPVVGPSLGGFITTYFSWHWI 168 LQG+ MVP+ + +++ K K A++ A+VAPV GP+LGG+IT ++W WI Sbjct: 121 LQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWI 180 Query: 169 FLINVPFGIAALTAVLRIV-PNERGNQRKPLDMIGFFLSGTALTSLLYGTELASHTDTNL 227 F IN+P G+ A L I E + P+D IG L T + + + L D + Sbjct: 181 FYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAA--FQIMLDKGKDLDW 238 Query: 228 LVAMLFIAGGLV-LGVFSY--RHGMRREHPLLD 257 + + G++ L F+Y + +HP++D Sbjct: 239 FASPTIVTLGIIALIAFAYFVVWELNNDHPVVD 271 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,385,366 Number of extensions: 55589 Number of successful extensions: 358 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 336 Number of HSP's successfully gapped: 20 Length of query: 475 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 386 Effective length of database: 1,198,455 Effective search space: 462603630 Effective search space used: 462603630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)