BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0008.p (79 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313191|ref|YP_003777283.1| transmembrane protein [Herbaspi... 134 1e-33 gi|300309983|ref|YP_003774075.1| transmembrane protein [Herbaspi... 117 2e-28 >gi|300313191|ref|YP_003777283.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 134 bits (337), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 62/79 (78%), Positives = 70/79 (88%) Query: 1 LRLQLQERGNAANNTDVVQIDGELLGIETLEHDYLATIKFTGLIKEAPDASAEPFAEIWN 60 LR+QL ERG A N TDVVQ+D +LLGIET+ DYLAT++F GLIKEAPD SAEPFAEIWN Sbjct: 239 LRMQLTERGAAPNATDVVQLDAQLLGIETMAFDYLATVRFQGLIKEAPDVSAEPFAEIWN 298 Query: 61 LSKPVNGTGGWVLAGIQQV 79 LSKP+NG+GGWVLAGIQQV Sbjct: 299 LSKPLNGSGGWVLAGIQQV 317 >gi|300309983|ref|YP_003774075.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 242 Score = 117 bits (293), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 54/79 (68%), Positives = 64/79 (81%) Query: 1 LRLQLQERGNAANNTDVVQIDGELLGIETLEHDYLATIKFTGLIKEAPDASAEPFAEIWN 60 LRLQ+QERG A+N TDVV +D E+LG+ET+ +DYLA++KF G IKE P AS FAEIWN Sbjct: 159 LRLQIQERGAASNQTDVVSLDAEVLGVETVGNDYLASVKFFGFIKEDPTASPAQFAEIWN 218 Query: 61 LSKPVNGTGGWVLAGIQQV 79 LSKP G GGWVLAGIQQ+ Sbjct: 219 LSKPAAGQGGWVLAGIQQI 237 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 281,744 Number of extensions: 9889 Number of successful extensions: 16 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 16 Number of HSP's successfully gapped: 2 Length of query: 79 Length of database: 1,619,870 Length adjustment: 50 Effective length of query: 29 Effective length of database: 1,383,120 Effective search space: 40110480 Effective search space used: 40110480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 123 (52.0 bits)