BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0007.p (131 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313191|ref|YP_003777283.1| transmembrane protein [Herbaspi... 65 2e-12 gi|300309983|ref|YP_003774075.1| transmembrane protein [Herbaspi... 57 5e-10 >gi|300313191|ref|YP_003777283.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 64.7 bits (156), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 50/100 (50%), Positives = 63/100 (63%), Gaps = 4/100 (4%) Query: 3 PKPASPWKGILGGALLGLGLGALLSHFGLGGAMASMLSTILMVALLAFVAMFLFRMFTRK 62 PKPASPW+ +LGGALLG GLGAL+SHFG+GGAM +L ++L +ALL +FL RMF R Sbjct: 74 PKPASPWRNVLGGALLGFGLGALMSHFGIGGAMGGLLGSLLTIALLCAAVIFLIRMFAR- 132 Query: 63 NGGNSGAAGNNGF-TPQPAYASAPELAVHTPEIGSRIEPV 101 G N + TP P + A TPEIGS + + Sbjct: 133 --GREAQNNNPAYATPMPGVSPIGSAAGATPEIGSGLRDI 170 >gi|300309983|ref|YP_003774075.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 242 Score = 57.0 bits (136), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 52/91 (57%), Positives = 62/91 (68%), Gaps = 5/91 (5%) Query: 9 WKGILGGALLGLGLGALLSHFGLGGAMASMLSTILMVALLAFVAMFLFRMFTRKNGGNSG 68 WKG+LGGALLGLGLGALLSHFGLGGA+ASM+STIL VAL+A MF+ RMF RK+ Sbjct: 2 WKGLLGGALLGLGLGALLSHFGLGGALASMISTILTVALIALAIMFVVRMFRRKS----- 56 Query: 69 AAGNNGFTPQPAYASAPELAVHTPEIGSRIE 99 A A A + A TP+IGS ++ Sbjct: 57 EASQPAPAWAGAAPQAADNASATPQIGSMLK 87 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.140 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 330,434 Number of extensions: 10917 Number of successful extensions: 44 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 43 Number of HSP's successfully gapped: 2 Length of query: 131 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 56 Effective length of database: 1,264,745 Effective search space: 70825720 Effective search space used: 70825720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)