BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0003.p (610 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309610|ref|YP_003773702.1| cation/multidrug efflux protein... 347 2e-96 gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 87 5e-18 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 76 9e-15 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 65 2e-11 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 61 2e-10 >gi|300309610|ref|YP_003773702.1| cation/multidrug efflux protein [Herbaspirillum seropedicae SmR1] Length = 1047 Score = 347 bits (889), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 208/546 (38%), Positives = 311/546 (56%), Gaps = 65/546 (11%) Query: 63 AXAKSAAGEYTFSMFSVNALALLISWLVAVVFTPYIGVLLLKVKPAANAD---------G 113 A AKS GEYT S+F V+A+ALL+SWL AVV P +G +LK KP A D G Sbjct: 455 ALAKSGTGEYTRSIFEVSAIALLLSWLAAVVLIPLLGYHMLKEKPGALTDEQIAANPHAG 514 Query: 114 H------HELFDTPFYTRFRKLVSWCVEWRKTTILLTLAAFMLGLFGFRFIEQQFFPDSS 167 H H ++ T YT R+ V+WCV +R ++ T F+ L F + QQFFP S Sbjct: 515 HDEEDHDHPIYQTRIYTWLRRSVAWCVAFRGRVVIATTVLFIGSLAAFALVPQQFFPSSD 574 Query: 168 RPELMIELWLPEGSAFKATEAQAKKFEAFIRKQDGVESVTSYVGTGSPRFYLPLDQIFPA 227 RPEL+I+L LPE ++F+AT+ +A + EA ++ + ++ S+VG G+PRFYLPLDQ + Sbjct: 575 RPELLIDLHLPERASFEATQREAVRMEALLKDRPQIDHYVSFVGVGAPRFYLPLDQQLAS 634 Query: 228 PRAADSSKAQDSIAANVPLVIFIIFTLLMLQLHSFSRAMLVFLTGPLGVAGAAVALLVLH 287 P A F++ + + +RA L PL Sbjct: 635 PNFAQ----------------FVVTAKSVEDREALARA----LEEPL------------R 662 Query: 288 RPFGFVAQLGVIALFGMIIRNSVILIDQIASGPPVPYAVQFRVTGTEVDKVRAIADQVKT 347 R F G+ R + ++ +GPPV + VQFR++G ++ KVRAIA+QV Sbjct: 663 RQFS-----------GVRTR-----LTRLENGPPVGFPVQFRISGEQIPKVRAIAEQVAA 706 Query: 348 VMNRNPNVIGLNDNWNE-SVKALRIDLDQDKLRVLGIGSQTVMRTVNTLLTGTAVGQYRE 406 M + + +W+E + +++ ++DQ + LG+ SQ + + L+G + QYRE Sbjct: 707 EMRKESRATNIQFDWDEPAQRSVSFEIDQKRAAQLGLTSQQISNFLAMQLSGYTITQYRE 766 Query: 407 DDKLIDILVRQPLEERATIDALNQANIPTASGKSVPLAQVARVSLAWEPGVVWREDREWA 466 DKLI + +R P ER L ++PTA+G VPLA + + + E GV+W +R+ Sbjct: 767 RDKLISVDLRAPRSERVDPARLATLSMPTANGP-VPLATLGQFNNTLEYGVIWERNRQPT 825 Query: 467 ITVQSDVADGIQGPTVSSQISPQLDAIRARLPAGYAIELAGAAADSSKAQDSIAANVPLV 526 ITV +DV G+QG V++ ++ +L+A+RA LP GY I++ G +++KAQ SI+A +PL+ Sbjct: 826 ITVLADVRPGVQGIDVTNDVNQKLNALRAELPVGYRIDVGGPVEENAKAQSSISAQMPLM 885 Query: 527 IFIIFTLLMLQLHSFSRAMLVFLTGPLGVAGAAVALLVLHRPFGFVAQLGVIALFGMIIR 586 + + LLM+QL SF R M+V +T PLG+ G ALL+ +PFGFVA LG IA+ G+I+R Sbjct: 886 VIAVLLLLMVQLQSFGRTMMVVMTAPLGLIGVVGALLLFGQPFGFVAMLGTIAMLGIIMR 945 Query: 587 NSVILI 592 NSVIL+ Sbjct: 946 NSVILV 951 Score = 85.9 bits (211), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 47/85 (55%), Positives = 65/85 (76%) Query: 232 DSSKAQDSIAANVPLVIFIIFTLLMLQLHSFSRAMLVFLTGPLGVAGAAVALLVLHRPFG 291 +++KAQ SI+A +PL++ + LLM+QL SF R M+V +T PLG+ G ALL+ +PFG Sbjct: 870 ENAKAQSSISAQMPLMVIAVLLLLMVQLQSFGRTMMVVMTAPLGLIGVVGALLLFGQPFG 929 Query: 292 FVAQLGVIALFGMIIRNSVILIDQI 316 FVA LG IA+ G+I+RNSVIL+DQI Sbjct: 930 FVAMLGTIAMLGIIMRNSVILVDQI 954 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 86.7 bits (213), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 68/274 (24%), Positives = 127/274 (46%), Gaps = 8/274 (2%) Query: 326 VQFRVTGTEVDKVRAIADQVKTVMNRNPNVIGLNDNWNESVKALRIDLDQDKLRVLGIGS 385 V +V G ++ + A ++ V+ + P + + L +++D++K G+ Sbjct: 685 VAVKVFGDDMKVLNDTASKISAVLEKIPGATEVKIEQTTGLPMLTVEIDREKTARYGLNV 744 Query: 386 QTVMRTVNTLLTGTAVGQYREDDKLIDILVRQPLEERATIDALNQANI--PTASGKS--- 440 + TV T + G G + D+ I+VR P + R ++A+ + I P G + Sbjct: 745 SDIQDTVATAIGGKEAGTMFQGDRRFGIVVRLPDQARTDLEAIRRLPIALPPQEGATRVS 804 Query: 441 -VPLAQVARVSLAWEPGVVWREDREWAITVQSDVADGIQGPTVSSQISPQLDAIRARLPA 499 VPL +VA +++A P + RED + I V ++V G V+ + QL + ++PA Sbjct: 805 YVPLGEVASLTIAPGPNQISREDGKRRIVVSANVRGRDLGGFVA-EAERQLQSA-VKVPA 862 Query: 500 GYAIELAGAAADSSKAQDSIAANVPLVIFIIFTLLMLQLHSFSRAMLVFLTGPLGVAGAA 559 GY G + A + +P+ + ++F LL + +LVF P + G Sbjct: 863 GYWTTWGGQFENLQSATQRLQIVIPVALLMVFVLLFAMFSNVKDGLLVFTGIPFALTGGI 922 Query: 560 VALLVLHRPFGFVAQLGVIALFGMIIRNSVILIA 593 +AL + P A +G IAL G+ + N +++I+ Sbjct: 923 LALAMRGIPMSISAAVGFIALSGVAVLNGLVMIS 956 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 75.9 bits (185), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 122/535 (22%), Positives = 213/535 (39%), Gaps = 78/535 (14%) Query: 74 FSMFSVNALALLISWLVAVVFTPYIGVLLLKVKPAANADGHHELFDTPFY-------TRF 126 FS+ V+A+AL S +VA+ TP + LLK P A F T F TR+ Sbjct: 472 FSITIVSAMAL--SVMVALTLTPALCATLLK--PHAGGHVQQGRFFTWFNRSFDRSATRY 527 Query: 127 RKLVSWCVEWRKTTILL-TLAAFMLGLFGFRFIEQQFFPDSSRPELMIELWLPEGSAFKA 185 + V+ ++ K +L+ L A + + R + F P+ + LM ++ LP G+ + Sbjct: 528 QGGVAGLLKRGKRGLLMYALIAVAMAVLFMR-LPTSFLPEEDQGVLMAQIQLPTGATAEQ 586 Query: 186 TEAQAKKF-EAFIRKQDGVESVTSYVGTG-------SPRFYLPLDQIFPAPRAADSSKAQ 237 T K E F+ K + VESV + VG G R ++ L + A S ++ Sbjct: 587 TATVVKTVQEYFMNKPESVESVLAIVGAGVGGNSQNGARAFIRLKD-WEQRTGAGQSASE 645 Query: 238 DSIAANVPLVIFIIFTLLMLQLHSFSRAMLVFLTGPLGVAGAAVALLVLHRPFGFVAQLG 297 AN L S R VFL P V G L + GF +L Sbjct: 646 LVRRANADL--------------SKIRGARVFLMNPPAVRG-------LGQSSGFDLELK 684 Query: 298 VIALFGMIIRNSVILIDQIASGPPVPYAVQFRVTGTEVDKVRAIADQVKTVMNRNPNVIG 357 + G D + +Q R ++ + A+ V+T G Sbjct: 685 DVGGVGH---------DAL---------LQARDQFLQLARSNALLANVRTT--------G 718 Query: 358 LNDNWNESVKALRIDLDQDKLRVLGIGSQTVMRTVNTLLTGTAVGQYREDDKLIDILVRQ 417 L+D LR+ +D K L I + + T+ T + GT V + + ++ + V+ Sbjct: 719 LDDT-----PQLRVAIDDRKADALSIATSDINATLATAMGGTYVNDFLSNGRVKKVYVQG 773 Query: 418 PLEERATIDALNQANIPTASGKSVPLAQVARVSLAWEPGVVWREDREWAITVQSDVADGI 477 R D LN+ + A+ + VP A + P ++ R + + + + A G+ Sbjct: 774 DSAFRMQADDLNRWYVRNAASQMVPFAAFMSSQWTYGPSLLERYNGVSSFEIVGEPAAGV 833 Query: 478 QGPTVSSQISPQLDAIRARLPAGYAIELAGAAADSSKAQDSIAANVPLVIFIIFTLLMLQ 537 T +++ + + A+LPAG E G++ + + + I +F L Sbjct: 834 SSGTAMAEV----EKLVAQLPAGIGFEWTGSSYQERLSGNQAPMLYAISILFVFLCLAAL 889 Query: 538 LHSFSRAMLVFLTGPLGVAGAAVALLVLHRPFGFVAQLGVIALFGMIIRNSVILI 592 S+S V L PLG+ G + + Q+G++ G+ +N+++++ Sbjct: 890 YESWSVPFAVILAVPLGIIGTVLLTGIFGMSNDVYFQVGLLTTVGLSAKNAILIV 944 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 65.1 bits (157), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 60/259 (23%), Positives = 117/259 (45%), Gaps = 15/259 (5%) Query: 336 DKVRAIADQVKTVMNRNPNVIGLNDNWNESVKALRIDLDQDKLRVLGIGSQTVMRTVNTL 395 +++ +A Q K + P GL D L++D+D+DK LG+ T+ ++T Sbjct: 696 NQILGMASQSKVLAGVRPE--GLED-----APQLQLDIDRDKANALGVPFSTINSVLSTA 748 Query: 396 LTGTAVGQYREDDKLIDILVRQPLEERATIDALNQANIPTASGKSVPLAQVARVSLAWEP 455 L + V + + ++V+ + R + L +SG VP + A V+ W Sbjct: 749 LGSSYVNDFPNLGRQQRVIVQADAKRRLQPEDLTNLYARNSSGTMVPFS--AFVTAKWIN 806 Query: 456 GVVW--REDREWAITVQSDVADGIQGPTVSSQISPQLDAIRARLPAGYAIELAGAAADSS 513 G V R + A+ + A G + + ++++ A+LP G+ E G + + Sbjct: 807 GPVQLIRYNGYPAMKIAGGAAPG----RSTGEAMAEMESFVAKLPPGFGFEWTGQSLEEK 862 Query: 514 KAQDSIAANVPLVIFIIFTLLMLQLHSFSRAMLVFLTGPLGVAGAAVALLVLHRPFGFVA 573 + + A L + +F +L S S + V L PLG+ GA + + + P Sbjct: 863 TSGNQAPALYALSLIAVFLVLAALYESTSIPLAVMLVVPLGILGALLGVTLRAMPNDVYF 922 Query: 574 QLGVIALFGMIIRNSVILI 592 ++G+IA+ G+ +N++++I Sbjct: 923 KVGMIAVVGLSAKNAILII 941 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 61.2 bits (147), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 43/225 (19%), Positives = 107/225 (47%), Gaps = 4/225 (1%) Query: 368 ALRIDLDQDKLRVLGIGSQTVMRTVNTLLTGTAVGQYREDDKLIDILVRQPLEERATIDA 427 ++++++D+ K LG+ ++ T++ + V + ++ ++++ R ++ Sbjct: 719 SVQLEIDRHKAAALGVSFSSISDTLSAAMGSFYVNDFPNAGRMQQVILQAQASARMQLED 778 Query: 428 LNQANIPTASGKSVPLAQVARVSLAWEPGVVWREDREWAITVQSDVADGIQGPTVSSQIS 487 + + + SG VPLA++ + S P + R A+ + A G+ S + Sbjct: 779 VMKLYVRNQSGGMVPLAELVKASWREAPTQLQRYQGFPAVRISGSAAPGV----ASGEAM 834 Query: 488 PQLDAIRARLPAGYAIELAGAAADSSKAQDSIAANVPLVIFIIFTLLMLQLHSFSRAMLV 547 +++ + +LP G+A+ G + + + L + ++F +L S+S + V Sbjct: 835 AEMERLAQQLPPGFAVAWTGQSLQERLSAAQAPMLMALSMLVVFLVLAALYESWSIPLSV 894 Query: 548 FLTGPLGVAGAAVALLVLHRPFGFVAQLGVIALFGMIIRNSVILI 592 L PLG+ GA A+++ P ++G+I + G+ +N+++++ Sbjct: 895 MLVVPLGLVGAVAAVILRDMPNDVFFKVGMITVIGLSAKNAILIV 939 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,678,771 Number of extensions: 64699 Number of successful extensions: 298 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 293 Number of HSP's successfully gapped: 10 Length of query: 610 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 519 Effective length of database: 1,188,985 Effective search space: 617083215 Effective search space used: 617083215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)