BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0040.p (705 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 1218 0.0 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 169 7e-43 gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 162 9e-41 gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729... 157 3e-39 gi|300313029|ref|YP_003777121.1| two component hybrid sensor his... 154 2e-38 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 1218 bits (3152), Expect = 0.0, Method: Compositional matrix adjust. Identities = 587/703 (83%), Positives = 641/703 (91%), Gaps = 4/703 (0%) Query: 1 MGKAVEPTQMYLIGYQYVFSNPALVLGAVALFVIVSQVKINVTNAYAGSLAWSNFFARLT 60 M +AVEPTQMYL+GYQYVF NPAL LGAV LFV+VSQ+KINVTNAYAGSLAWSNFFARLT Sbjct: 295 MARAVEPTQMYLVGYQYVFGNPALALGAVVLFVVVSQIKINVTNAYAGSLAWSNFFARLT 354 Query: 61 HSHPGRVVWLVFNVLIAVLLMELGVFNALEHVLALYSLVAISWIGAVVSDLVINKPLKLS 120 HSHPGRVVWLVFNVLIAVLLMELGVFNALEHVLALYSLVAISWIGAVV+DLVINKPL+LS Sbjct: 355 HSHPGRVVWLVFNVLIAVLLMELGVFNALEHVLALYSLVAISWIGAVVADLVINKPLRLS 414 Query: 121 PAHIEFKRAHLYDINPVGVGSMAVASILSAIAMSGVLGKLAAAMSPFIALGTAMLAAPLI 180 PAHIEFKRAHLYD+NPVGVG+MA+A++LS +AMSGVLGK AAM+PFIALGTA+ +APLI Sbjct: 415 PAHIEFKRAHLYDVNPVGVGAMAIATLLSTLAMSGVLGKTVAAMAPFIALGTALASAPLI 474 Query: 181 ALATKSRYYIARQDTLAEHSNLGDVHVHAQ--LDCVICGNAFETPDMAHCPAYQGAICSL 238 ALAT SRYYIAR+DTLAE + AQ L C ICGNAFETPDMAHCPAYQGAICSL Sbjct: 475 ALATGSRYYIARRDTLAEQNYRPVTPGQAQPVLACAICGNAFETPDMAHCPAYQGAICSL 534 Query: 239 CCSLDARCNDRCKTPAARLPNQVFNTLQKLLPSRFTLKLNTRIGHYLIVFGLLSGGLAVV 298 CCSLDARC DRCKTP ARLPNQ+ +TL++L+P+RF +KLNTRIGHYL VFGLL+GGLAVV Sbjct: 535 CCSLDARCKDRCKTPRARLPNQILHTLRQLIPARFAVKLNTRIGHYLSVFGLLAGGLAVV 594 Query: 299 LWMLYFQQMAGISGLTTSGPDSIQSIFIKLFCTLLVLIGVGSWWLILTNESRSVAHEESE 358 LWMLYFQQMA +SG P S++S+FIKLFC LLV++GVG+WWL+LTNESR+VA EESE Sbjct: 595 LWMLYFQQMADLSGSGLDAPASLRSVFIKLFCLLLVVVGVGAWWLVLTNESRAVAQEESE 654 Query: 359 RQTNLLLQEIEAHKQTDAELQRAKEAAESANLAKSRFVTGMSHELRTPLNSILGYAQILH 418 RQTNLLLQEIEAHKQTDAELQRAKEAAESANLAKSRFV GMSHELRTPLNSILGYAQILH Sbjct: 655 RQTNLLLQEIEAHKQTDAELQRAKEAAESANLAKSRFVAGMSHELRTPLNSILGYAQILH 714 Query: 419 VDATIPPARKDAIAVIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELALSEFLEQIAGM 478 VDATIPPARKDAI VIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELAL FLEQI GM Sbjct: 715 VDATIPPARKDAIGVIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELALEAFLEQIVGM 774 Query: 479 FAPQAEQKGLSFRFEKTGRIPHIVHADQKRLRQILINLLGNAVKFTDRGGVTVRVRYLRE 538 FAPQAEQKGL FRF+K+GR+P IVHADQKRLRQILINLLGNAVKFTD GGVT+R+RY RE Sbjct: 775 FAPQAEQKGLHFRFDKSGRLPQIVHADQKRLRQILINLLGNAVKFTDHGGVTLRLRYARE 834 Query: 539 IAYFDIIDTGIGIAEDDLDRIFLPFERSNAANLREDIGTGLGLAISSMLTHIMGGELAVR 598 IAYFD+IDTGIGIA+DD +RIFLPFERS+AANLREDIGTGLGLAIS ML HIMGGEL+V+ Sbjct: 835 IAYFDVIDTGIGIADDDRERIFLPFERSSAANLREDIGTGLGLAISRMLVHIMGGELSVQ 894 Query: 599 SEVGSGSSFHLKIYLPEVHVPRPRLKLEDQMSGYIGPCKRVLVVDDQASQRLLLKEMLLP 658 S+VG GSSFHLKIYLPEVHVPRPR+KLEDQMSGY GP + +LVVDDQASQR LLK+ML+P Sbjct: 895 SQVGQGSSFHLKIYLPEVHVPRPRIKLEDQMSGYAGPSRHILVVDDQASQRRLLKDMLMP 954 Query: 659 LGFEVIEADGGIACLEALAHGTPMPDLVLLDIAMPVMDGWSVA 701 LGF V EAD G+AC+E L H +PDLVLLDIAMP MDGWSVA Sbjct: 955 LGFTVSEADSGLACMETLGHA--LPDLVLLDIAMPQMDGWSVA 995 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 169 bits (428), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 127/396 (32%), Positives = 202/396 (51%), Gaps = 61/396 (15%) Query: 358 ERQTNLL-LQEIEAHKQTDAELQRAKEAAESANLAKSRFVTGMSHELRTPLNSILGYAQI 416 E QT LL LQ+ + K +++A E E AN KS F+ MSHELRTPLNS L A++ Sbjct: 441 EEQTQLLELQKDDLAKTQVVLVEKATEL-ERANQYKSEFLANMSHELRTPLNSTLILAKL 499 Query: 417 L--HVDATIPPARKDAIAVIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELALSEFLEQ 474 L + + + I +G LL+LI+ +LD++K+EAGK+ + + L++ ++ Sbjct: 500 LADNKQGNLSDEQVRFAQTISSAGNDLLALINDILDLSKMEAGKLDIVPESFPLTKLADE 559 Query: 475 IAGMFAPQAEQKGLSFRFEKTGRIPHIVHADQKRLRQILINLLGNAVKFTDRGGVTVRVR 534 ++ FA A+QKGL F+ E +P + D +RL QIL NLL NA+KFT++G V++R+ Sbjct: 560 LSASFAITAQQKGLGFKVEVEPGVPQRMETDIQRLGQILKNLLSNAIKFTEKGEVSLRI- 618 Query: 535 YLR---EIAYFDIIDTGIGIAEDDLDRIFLPFERSNAANLREDIGTGLGLAISSMLTHIM 591 Y IA F + DTGIGI ED IF F +++ + R+ GTGLGL+IS L H++ Sbjct: 619 YPDAGGRIA-FAVRDTGIGIGEDQHQFIFEAFRQADGSTHRKYGGTGLGLSISRDLAHLL 677 Query: 592 GGELAVRSEVGSGSSFHLKI----------------------------------YLPEVH 617 GG++ V S +G GS F L + P+V Sbjct: 678 GGDIRVTSRLGEGSIFTLSLPPLYSSEQAVSNTAPEPTPAAAAAASTLQAAHVATAPDVR 737 Query: 618 VPR----------PRLKLEDQMSGYIGPCKRVLVVDDQASQRLLLKEMLLPLGFEVI--- 664 + R + ++D + +LV++D A+ ++L+++ +GF+ + Sbjct: 738 INRTPAAAAPQRLQEISIDDDRDRIETSSRLILVIEDDAAFAMILRDLAREMGFQCVVTH 797 Query: 665 EADGGIACLEALAHGTPMPDLVLLDIAMPVMDGWSV 700 A+ G+A E P +L+D+ +P G V Sbjct: 798 SANEGVAAAEMY-----RPSAILMDMNLPDFSGLGV 828 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 162 bits (410), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 96/261 (36%), Positives = 156/261 (59%), Gaps = 6/261 (2%) Query: 364 LLQEIEAHKQTDAELQRAKEAAESANLAKSRFVTGMSHELRTPLNSILGYAQILHVDATI 423 LL+ + + +T +L +AKEAA+ A L + F++ MSHE+RTP+N++LG A +L + Sbjct: 59 LLRLLRENARTAEKLVQAKEAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAGLLR-STRL 117 Query: 424 PPARKDAIAVIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELALSEFLEQIAGMFAPQA 483 + + I S LL +++ +LD +KI+ GKM +A E L++ +E A + +A Sbjct: 118 DTQQAEFAKAIEDSAHALLGIVNDVLDFSKIDVGKMDIAVVEFDLAKVVEASAEALSFKA 177 Query: 484 EQKGLSFRFEKTGRIPHIVHADQKRLRQILINLLGNAVKFTDRGGVTVRVRYLREIA--- 540 +K + R+P + D R+RQIL+NLLGNA+KFT G V +RV+ L+ + Sbjct: 178 REKRVRLLAHLDSRLPPRLVGDPGRIRQILLNLLGNALKFTHAGEVLLRVQALQVTSDHC 237 Query: 541 --YFDIIDTGIGIAEDDLDRIFLPFERSNAANLREDIGTGLGLAISSMLTHIMGGELAVR 598 F++ D+GIG+ ED L R+F PF +++++ R+ GTGLGL+I L +MGG + V Sbjct: 238 RVRFEVRDSGIGMDEDTLRRLFSPFAQADSSVTRQYGGTGLGLSICKRLVELMGGSIGVE 297 Query: 599 SEVGSGSSFHLKIYLPEVHVP 619 S +G+GS F ++ +P P Sbjct: 298 SALGTGSCFWFELGIPVTTGP 318 >gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729 [Herbaspirillum seropedicae SmR1] Length = 765 Score = 157 bits (397), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 116/379 (30%), Positives = 188/379 (49%), Gaps = 54/379 (14%) Query: 371 HKQTDAEL-QRAKEAAESANLAKSRFVTGMSHELRTPLNSILGYAQILHVDATIPPARKD 429 ++ DA L R KE A+ AN AKS F+ +SHE+RTP+N ILG +++L +++ + ++D Sbjct: 326 RREEDALLATRLKEEADHANEAKSHFLAMVSHEIRTPMNGILGLSELL-MESGLSSKQRD 384 Query: 430 AIAVIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELALSEFLEQIAGMFAPQAEQKGLS 489 I + L+ +I+ +LD +KIE+GK+ L +++++ ++ P ++K + Sbjct: 385 YADSIHGATGGLVRIINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPSLQRKDVQ 444 Query: 490 FRFEKTGRIPHIVHADQKRLRQILINLLGNAVKFTDRGGVTVRVRYLREIA-------YF 542 F V D RL+Q+L NLL NA+KFT G V + + ++ + +F Sbjct: 445 FDLHLDCPENLSVVGDPARLKQVLGNLLSNAIKFTQAGHVQLSMSARQDESGPSRWRLFF 504 Query: 543 DIIDTGIGIAEDDLDRIFLPFERSNAANLREDIGTGLGLAISSMLTHIMGGELAVRSEVG 602 I DTGIGI+ + L ++F PF +++++ R GTGLGLAIS L +MGG +A +S G Sbjct: 505 AITDTGIGISPEALQQLFRPFAQADSSISRRFGGTGLGLAISKNLVELMGGTIACQSIPG 564 Query: 603 SGSSFHLKIYLPEVHVPRPR--------------------LKLEDQMSGYIGPCK----- 637 + F +I PE P P L + G + P Sbjct: 565 ESTRFSFEILCPEASTPAPEPVSEAAAPAPATALATTAPALPPAAEAPGTLMPPDNAPQP 624 Query: 638 ------------------RVLVVDDQASQRLLLKEMLLPLGFEVIEADGGIACLEALAHG 679 R+L+ +D R LL+ +L G + E + G+ +EA+ G Sbjct: 625 PSNVDASATVLPSLLAGARILMAEDTEINRQLLRILLARKGCILQEVENGLQAVEAIRTG 684 Query: 680 TPMPDLVLLDIAMPVMDGW 698 DLVL+D MP+MDG+ Sbjct: 685 --HYDLVLMDCMMPIMDGY 701 >gi|300313029|ref|YP_003777121.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1524 Score = 154 bits (389), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 124/350 (35%), Positives = 179/350 (51%), Gaps = 36/350 (10%) Query: 381 AKEAAESANLAKSRFVTGMSHELRTPLNSILGYAQILHVDATIP-PARKDAIAVIRRSGE 439 A+ AE AN AKS F+ +SHE+RTP+N +LG Q+L T P PA++D + S + Sbjct: 886 ARLKAEQANSAKSLFLASVSHEIRTPMNGVLGMIQLLQ--RTQPLPAQQDYLDKAEISAK 943 Query: 440 HLLSLIDGLLDIAKIEAGKMRLASDELALSEFLEQIAGMFAPQAEQKGLSFRFEKTGRIP 499 LL+L++ LLD +K++A +++L L F +A + A Q G+S ++P Sbjct: 944 SLLNLLNDLLDFSKMDAKQLQLDLQAFELERFGHNLAAVLAGSPPQAGVSLHIALDPQLP 1003 Query: 500 HIVHADQKRLRQILINLLGNAVKFTDRGGVTVRVRYL-----REIA-YFDIIDTGIGIAE 553 + D RL+Q+L+NL GNA+KFT +G + V L R +A ++ DTGIGIA Sbjct: 1004 RRLVGDAHRLQQVLVNLTGNALKFTHQGHIDVHFTRLDPLPGRTVALRVEVSDTGIGIAA 1063 Query: 554 DDLDRIFLPFERSNAANLREDIGTGLGLAISSMLTHIMGGELAVRSEVGSGSSFHLKIYL 613 D LD IF F ++ A+ R GTGLGLAIS L +MGG+L V SE G GS F + L Sbjct: 1064 DRLDDIFDAFTQAEASTTRRYGGTGLGLAISKHLVGLMGGQLQVSSEPGRGSRFWFDLQL 1123 Query: 614 P-------------EVHVPRPRLKLEDQMSGYIGPCK-------------RVLVVDDQAS 647 + P+P L+ +G P ++L+V+D Sbjct: 1124 AVDDDSVLGPAAADDAARPQP-LQAASHTAGSRAPETARSAPDAQCLQGVQILLVEDNPV 1182 Query: 648 QRLLLKEMLLPLGFEVIEADGGIACLEALAHGTPMPDLVLLDIAMPVMDG 697 R + E+L G +V AD G + DLVL+D+ MP MDG Sbjct: 1183 NRQVASELLALSGAQVTCADSGSMGVAMACDPARHFDLVLMDLQMPGMDG 1232 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,191,875 Number of extensions: 94393 Number of successful extensions: 538 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 489 Number of HSP's successfully gapped: 39 Length of query: 705 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 613 Effective length of database: 1,184,250 Effective search space: 725945250 Effective search space used: 725945250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 134 (56.2 bits)