BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0019.p (122 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312408|ref|YP_003776500.1| permease [Herbaspirillum serope... 140 2e-35 >gi|300312408|ref|YP_003776500.1| permease [Herbaspirillum seropedicae SmR1] Length = 376 Score = 140 bits (354), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 83/122 (68%), Positives = 104/122 (85%) Query: 1 LLAQDTQFTRGELLWRMALPLMASTLMLLAIPLSFVNPRVGRSMGLLVALLLFVVYSNTV 60 L+A++ + RGELLWR+ALP MA TLMLLAIPLSFVNPR GRS+GLLVALLLFVVYSNTV Sbjct: 255 LVAKNDGYARGELLWRIALPFMALTLMLLAIPLSFVNPRAGRSLGLLVALLLFVVYSNTV 314 Query: 61 SVMQSSVIQNRTSFLLAWWPVHLVVALLVIGLFFLRLKVNSRYHPAVIWSRLRRALFLKR 120 SV Q+SV Q R +F++AWWPVH+VV LL++G+F LRL +N YHP+ +WSR+RRA+ +R Sbjct: 315 SVFQASVAQGRATFMMAWWPVHVVVVLLIVGMFALRLNINHPYHPSRLWSRVRRAMTFQR 374 Query: 121 QA 122 +A Sbjct: 375 EA 376 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.333 0.140 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 339,303 Number of extensions: 11347 Number of successful extensions: 86 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 86 Number of HSP's successfully gapped: 1 Length of query: 122 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 48 Effective length of database: 1,269,480 Effective search space: 60935040 Effective search space used: 60935040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 125 (52.8 bits)