BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0006.p (422 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 709 0.0 gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 275 5e-75 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 150 2e-37 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 131 9e-32 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 127 2e-30 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 709 bits (1829), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/419 (80%), Positives = 376/419 (89%), Gaps = 4/419 (0%) Query: 3 SPDRAQYLDTEVPVTWLESNEPSYILYTSGTTGKPKGVQRDVGGYAVALAASMPYVFCGN 62 +P R Q+L +VPVTWLESN SYILYTSGTTGKPKGVQRDVGGYAVALA+SM ++FCG Sbjct: 213 APLRTQHLGQQVPVTWLESNASSYILYTSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQ 272 Query: 63 PGETYFCTSDIGWVVGHSYIVYGPLMAGMATILYEGLPVLAGDKPDGGIWWSLVEKYKVT 122 PGETYFCTSDIGWVVGHSYIVYGPL+AGMAT+LYEGLP+ +PD GIWWS+VEKYKVT Sbjct: 273 PGETYFCTSDIGWVVGHSYIVYGPLIAGMATVLYEGLPI----RPDAGIWWSIVEKYKVT 328 Query: 123 RMFSSPTAIRVLKKQPAECMTRHDLSSLKSLYLAGEPLDETTSKWISEALGVPVIDNYWQ 182 RMFS+PTAIRVLKKQP ECM R+D SSLK+LYLAGEPLDETTS WIS AL VPVIDNYWQ Sbjct: 329 RMFSAPTAIRVLKKQPPECMERYDTSSLKALYLAGEPLDETTSSWISGALKVPVIDNYWQ 388 Query: 183 TESGWPILSVAKGVDDKPTRLGSPGVPLYGYKMHIMNESTGALCGANEKGVVVIEGPLPP 242 TESGWPI+S+AKG+DDKPTRLGSPGVP+ GY++ I++E+TG CG ++KGVV IEGPLPP Sbjct: 389 TESGWPIISIAKGIDDKPTRLGSPGVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPP 448 Query: 243 GCMQTVYRDDERFVNTYWSNFKTQVYSTFDWGIRDEDGYYFILGRTDDVINVAGHRLGTR 302 GCMQTVY DDERFVNTYW + +VYSTFDWGIRD+DGYYFILGRTDDVINVAGHRLGTR Sbjct: 449 GCMQTVYGDDERFVNTYWRSLPREVYSTFDWGIRDKDGYYFILGRTDDVINVAGHRLGTR 508 Query: 303 EIEESISSHANVSEVAVVGVEDKLKGQVAIAFVIPKRADAVATAEARQALEAEIMRVVDK 362 EIEESISSH NVSEVAVVGVEDKLKGQVA AF IPK+A +AT E R+ALEAEIM VVDK Sbjct: 509 EIEESISSHPNVSEVAVVGVEDKLKGQVAFAFAIPKQAYTIATPEQRKALEAEIMAVVDK 568 Query: 363 QIGAVGRPSRVHFISLLPKTRSGKLLRRSIQAICEGRDPGDLTTIEDPNSLEQIKAALQ 421 QIGAVGRP+RV F+S LPKTRSGKLLRR+IQ+ICEGRDPGDL TIEDP +LEQ++A ++ Sbjct: 569 QIGAVGRPARVFFVSGLPKTRSGKLLRRAIQSICEGRDPGDLPTIEDPAALEQVRAVIK 627 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 275 bits (703), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 158/412 (38%), Positives = 244/412 (59%), Gaps = 20/412 (4%) Query: 18 WLESNEPSYILYTSGTTGKPKGVQRDVGGYAVALAASMPYVFCGNPGETYFCTSDIGWVV 77 W+++ P +ILYTSG+TGKPKGVQ GY + +M + F P + Y+CT+DIGW+ Sbjct: 258 WVDAEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFDIKPDDVYWCTADIGWIT 317 Query: 78 GHSYIVYGPLMAGMATILYEGLPVLAGDKPDGGIWWSLVEKYKVTRMFSSPTAIRVLKKQ 137 GH+YI YGPL G I++EG+P P+ G +W +V ++K T +++PTAIR L K Sbjct: 318 GHTYIAYGPLAVGATQIVFEGVPTY----PNAGRFWDMVARHKATIFYTAPTAIRSLIKA 373 Query: 138 PAECMTRH----DLSSLKSLYLAGEPLDETTSKWISEALG---VPVIDNYWQTESGWPIL 190 H DLSSL+ L GEP++ W + +G P++D +WQTE+G ++ Sbjct: 374 ADADEKVHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIVDTFWQTETGGHMI 433 Query: 191 SVAKGVDDKPTRLGSPGVPLYGYKMHIMNESTGALCGANEKGVVVIEGPLPPGCMQTVYR 250 S G P GS +PL G I++E+ + N G++V++ P P ++T++ Sbjct: 434 SPLPGA--TPQVPGSCTLPLPGITAAIVDETGNDIPNGN-GGILVVKRPW-PSMIRTIWN 489 Query: 251 DDERFVNTYW-SNFKTQVYSTFDWGIRD-EDGYYFILGRTDDVINVAGHRLGTREIEESI 308 D ERF +Y+ ++Y D +R+ E GY+ I GR DDV+NV+GHR+GT EIE ++ Sbjct: 490 DPERFKKSYFPEELGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESAL 549 Query: 309 SSHANVSEVAVVGVEDKLKGQVAIAFVIPKRADAVATAEARQALEAEIMRVVDKQIGAVG 368 +++ V+E AVVG D+ G+ AFV+ KR EA+Q + E+ V K+IG + Sbjct: 550 VANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGD-EAKQ-IAKELRDWVAKEIGPIA 607 Query: 369 RPSRVHFISLLPKTRSGKLLRRSIQAICEGRD-PGDLTTIEDPNSLEQIKAA 419 +P + F LPKTRSGK++RR ++ + +G D++T+E+P L+Q+K A Sbjct: 608 KPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEA 659 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 150 bits (379), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 124/394 (31%), Positives = 198/394 (50%), Gaps = 31/394 (7%) Query: 5 DRAQYLDTEVPVTWLESNEPSYILYTSGTTGKPKGV---QRDVGGYAVALAASMPYVFCG 61 DR E+ +++P+ I+YTSGTTGK KG R + G+ + S + Sbjct: 178 DRLHAAPAELDPVLTLADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVS--HDSFP 235 Query: 62 NPGETYFCTSDIGWVVGHSYIVYGPLMAGMATILYEGLPVLAG--DKPDGGIWWSLVEKY 119 G+ ++ +D W+ G L+ + LY G+ V+A +K D + L+ ++ Sbjct: 236 QEGDRFWTPADWAWI--------GGLLDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARH 287 Query: 120 KVTRMFSSPTAIRVLKKQPAECMTRHDLSSLKSLYLAGEPLDETTSKWISEALGVPVIDN 179 ++ +F PTA+++L+ A + D S L+S+ GE L + W EALGV + + Sbjct: 288 QIRNVFFPPTALKMLRGA-ATVRAQADFS-LRSVASGGETLGDDLIAWGREALGVTINEF 345 Query: 180 YWQTESGWPILSVAKGVDDKPTRLGSPGVPLYGYKMHIMNESTGALCGANEKGVVVIEGP 239 Y QTE L V+ P+ GS G + G+ + I++E G + G + I P Sbjct: 346 YGQTECN---LVVSSSSHCYPSVSGSMGRAVPGHVVQIVDEQ-GQVLPHGTVGNIAIRAP 401 Query: 240 LPPGCMQTVYRDDERFVNTYWSNFKTQVYSTFDWGIRDEDGYYFILGRTDDVINVAGHRL 299 P ++ +R++E + +F T D G DE GY LGR DDVI AG+R+ Sbjct: 402 DPVMFLR-YWRNEEATREKFAGDFLL----TGDLGSMDEQGYIRYLGRNDDVITSAGYRI 456 Query: 300 GTREIEESISSHANVSEVAVVGVEDKLKGQVAIAFVIPKRADAVATAEARQALEAEIMRV 359 G IEE + H V AVVGV+D L+ +V AFV+ K D V E AL+AE+ + Sbjct: 457 GPAAIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLK--DGVTPDE---ALKAELQQH 511 Query: 360 VDKQIGAVGRPSRVHFISLLPKTRSGKLLRRSIQ 393 V Q+ A P + F++ LP T +GK++R++++ Sbjct: 512 VRAQLAAHEYPRLISFVAALPTTATGKIMRKTLK 545 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 131 bits (330), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 105/380 (27%), Positives = 181/380 (47%), Gaps = 31/380 (8%) Query: 27 ILYTSGTTGKPKGVQRDVGGYAVALAASMPYVFCGNPGETYFCTSDIGWVVGHSYIVYGP 86 ++ TSGTTG KGV + A + M P + ++ +D GW G Y + GP Sbjct: 202 MMSTSGTTGAAKGVPVPLSALQ-AFSVYMREAVGLLPQDKFWNMADPGWAYGLYYAIIGP 260 Query: 87 LMAGMATILYEGLPVLAGDKPDGGIWWSLVEKYKVTRMFSSPTAIRVLKKQPAECMTRHD 146 L G E ++G + + + +T + +PTA R++ E Sbjct: 261 LAIGHGITFNEAAFTVSGT-------YETIRRLGITSLAGAPTAYRMMMAAGEEAAASVK 313 Query: 147 LSSLKSLYLAGEPLDETTSKWISEALGVPVIDNYWQTESGWPILSVAKGVDDKPTRLGSP 206 L+++ AGEPL+ +W + AL VP+ D+Y QTE G +++ + + GS Sbjct: 314 -GRLRAVSSAGEPLNAEVVRWFASALDVPIHDHYGQTELGM-VVNNHHALRHEVVP-GSA 370 Query: 207 GVPLYGYKMHIMNESTGALCGANEKGVVVIEGPLPPGCMQTVYRDDERFVNTYWSNFKTQ 266 G + GY++ +++E+ L GAN+ G + I+ + R + + YW Sbjct: 371 GFAMPGYRVVVLDENHQEL-GANQPGELAID----------IARSPLYWFSGYWKQDTPA 419 Query: 267 V----YSTFDWGIRDEDGYYFILGRTDDVINVAGHRLGTREIEESISSHANVSEVAVVGV 322 + YST D + +G +GR+DDVI AG+R+G ++E ++ H V++VAV+G+ Sbjct: 420 IAHGYYSTGDNVELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVIGL 479 Query: 323 EDKLKGQVAIAFVIPKRADAVATAEARQALEAEIMRVVDKQIGAVGRPSRVHFISLLPKT 382 D + ++ AFV+ +A AL ++ + V +++ A P + F+ LPKT Sbjct: 480 PDPERTEIVKAFVVLSD-----QFKASDALREQLAQHVKRRLSAHAYPRAIEFLDALPKT 534 Query: 383 RSGKLLRRSIQAICEGRDPG 402 SGKL R ++ + R G Sbjct: 535 PSGKLQRFVLRKMEADRRAG 554 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 127 bits (319), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 108/387 (27%), Positives = 183/387 (47%), Gaps = 36/387 (9%) Query: 19 LESNEPSYILYTSGTTGKPKGVQRDVGGYAVALAASMPYVFCGNPGETYFCTSDIGWVVG 78 ++ ++P + YTSGTTG PK G A + + + PG T+ S + + Sbjct: 191 VDYDDPLWFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLM---PGLTHQSRSLVVAPLS 247 Query: 79 HSYIVYGPLMA--GMATILYEGLPVLAGDKPDGGIWWSLVEKYKVTRMFSSPTAIRVLKK 136 H V+ + G A+IL ++ + W LVEKY+V +F+ PT +++L + Sbjct: 248 HGAGVHAVINTARGAASILLSSERLVVEEA------WQLVEKYRVDNLFTVPTIVKILVE 301 Query: 137 QPAECMTRHDLSSLKSLYLAGEPLDETTSKWISEALGVPVIDNYWQTESGWPILSVAKGV 196 E + R+D SSLK + AG P+ + + LG ++ Y E I + + Sbjct: 302 D--ESVDRYDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYGLGEVTGNITFLPPYL 359 Query: 197 ----DDKP-TRLGSPGVPLYGYKMHIMNESTGALCGANEKGVVVIEGPLPPGCMQTVYRD 251 D P R+G+ G+P G ++ I+N+ G E G + + GP V+ Sbjct: 360 HFEDDAHPQARVGTCGMPRTGMQIAILNDD-GQELAPFETGEICVRGP-------AVFMG 411 Query: 252 DERFVNTYWSNFKTQVYSTFDWGIRDEDGYYFILGRTDDVINVAGHRLGTREIEESISSH 311 FK + T D G D+DG+ +I GR+ D+ G + REIEE++ +H Sbjct: 412 YHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPREIEEALLTH 471 Query: 312 ANVSEVAVVGVEDKLKGQVAIAFVIPKRADAVATAEARQALEAE-IMRVVDKQIGAVGRP 370 VSEVAV+GV D G+ +A ++ K A Q ++ E ++ ++ +IG P Sbjct: 472 PAVSEVAVLGVPDPKWGESGLAVIVCK---------AGQQVDGEALLAHLESRIGKYKWP 522 Query: 371 SRVHFISLLPKTRSGKLLRRSIQAICE 397 R + +PK+ GK++++ I+A+ E Sbjct: 523 RRFIYWDSMPKSGYGKIVKKQIKALLE 549 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,421,328 Number of extensions: 63319 Number of successful extensions: 208 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 180 Number of HSP's successfully gapped: 13 Length of query: 422 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 334 Effective length of database: 1,203,190 Effective search space: 401865460 Effective search space used: 401865460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)