BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0014.0001.p
         (358 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspiri...   568   e-163
gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspiri...   253   2e-68

>gi|300311598|ref|YP_003775690.1| aconitate hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 873

 Score =  568 bits (1464), Expect = e-163,   Method: Compositional matrix adjust.
 Identities = 272/333 (81%), Positives = 301/333 (90%), Gaps = 6/333 (1%)

Query: 1   MFFIDQQTIDYLKLTGREDEQVRLVETYAKEAGLWADSLASAQYERTLKFDLSTVVRTLA 60
           MF IDQQTIDYLKLTGREDEQV+LVETYAKEAGLW+D+LA  +YER L+FDLSTVVRTLA
Sbjct: 304 MFSIDQQTIDYLKLTGREDEQVKLVETYAKEAGLWSDTLAKVEYERVLRFDLSTVVRTLA 363

Query: 61  GPSNPHRRLPVSALAERGIAVDLDKAQAQEAEGLMPDGAVIIAAITSCTNTSNPRNVIAA 120
           GPSNPH+RLPVS LA +GIA      + +   G MPDGAVIIAAITSCTNTSNPRNVIAA
Sbjct: 364 GPSNPHKRLPVSELAAQGIA-----GKVENEPGKMPDGAVIIAAITSCTNTSNPRNVIAA 418

Query: 121 GLLARNANKLGLTRKPWVKSSLAPGSKTVELYLDEAGLSTELQKLGFGIVAFACTTCNGM 180
           GLLARNAN+LGL RKPWVKSSLAPGSK VELYL+EAGL+ +L+KLGFGIVAFACTTCNGM
Sbjct: 419 GLLARNANRLGLARKPWVKSSLAPGSKAVELYLEEAGLTADLEKLGFGIVAFACTTCNGM 478

Query: 181 SGALDPVIQKEIIERDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIRFD 240
           SGALDP IQ+EII+RDLY+TAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIRFD
Sbjct: 479 SGALDPKIQQEIIDRDLYSTAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIRFD 538

Query: 241 IEKDVLTVV-DGKEIRLKDIWPSDEEIDAVVKSSVKPAQFRNVYTPMFAVQADKGEAAAP 299
           IE+DVL V  +G+EIRLKDIWPSDEEIDAVV ++VKP QFRNVYTPMFA + D+ ++ +P
Sbjct: 539 IEQDVLGVTAEGREIRLKDIWPSDEEIDAVVTAAVKPQQFRNVYTPMFAARVDRSQSVSP 598

Query: 300 LYDWRPMSTYIRRPPYWEGALAGERTMTGMRAL 332
           LYDWRP STYIRRPPYWEGALAGERT++GMR L
Sbjct: 599 LYDWRPQSTYIRRPPYWEGALAGERTLSGMRPL 631


>gi|300312283|ref|YP_003776375.1| aconitate hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 903

 Score =  253 bits (645), Expect = 2e-68,   Method: Compositional matrix adjust.
 Identities = 157/365 (43%), Positives = 211/365 (57%), Gaps = 35/365 (9%)

Query: 1   MFFIDQQTIDYLKLTGREDEQVRLVETYAKEAGLWADSLASA-QYERTLKFDLSTVVRTL 59
            F +D  TI+Y K TGR   ++   E Y K   L+    A    Y + +  DL TV  +L
Sbjct: 313 FFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGVPKAGQIDYTQEVSLDLGTVAPSL 372

Query: 60  AGPSNPHRRLPVSAL------------AERGI---AVDLDKAQAQEAEGL-MPDGAVIIA 103
           AGP  P  R+ +  +            AE G    A DL  A  + A+G+ + +G V+IA
Sbjct: 373 AGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAEDLG-ASYKNADGVDLHNGDVLIA 431

Query: 104 AITSCTNTSNPRNVIAAGLLARNANKLGLTRKPWVKSSLAPGSKTVELYLDEAGLSTELQ 163
           AITSCTNTSNP  ++AAGLLA+ A + GL   P +K+SLAPGS+ V  YL+ AGL   L+
Sbjct: 432 AITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKYLEAAGLLPYLE 491

Query: 164 KLGFGIVAFACTTCNGMSGALDPVIQKEIIERDLYATAVLSGNRNFDGRIHPYAKQAFLA 223
           KLGFG+ A+ CTTC G +G L P + + I++ D+ A AVLSGNRNF+ RIHP  +  FLA
Sbjct: 492 KLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKNDVVAAAVLSGNRNFEARIHPNIRANFLA 551

Query: 224 SPPLVVAYAIAGTIRFDIEKD-VLTVVDGKEIRLKDIWPSDEEIDAVVKSSVKPAQFRNV 282
           SPPLVVAYAIAG +  D+  + V     GK+I L DIWP+ +EI+ ++K ++    F+  
Sbjct: 552 SPPLVVAYAIAGNVTRDLMTEPVGKGKGGKDIYLGDIWPTSQEIEKLLKFAMNAKTFKEN 611

Query: 283 Y-----TPMFAVQADKGEAAAPLYDWRPMSTYIRRPPYWEG-------ALAGERTMTGMR 330
           Y      P    +A KG A   +Y+W P STYI  PP++E        A AG   +TG R
Sbjct: 612 YADVKGAPGKLWEAIKGVAKGEVYNW-PSSTYIAEPPFFENFSEEPKAAAAG---ITGAR 667

Query: 331 ALPXF 335
           AL  F
Sbjct: 668 ALGVF 672


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.133    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,051,467
Number of extensions: 42803
Number of successful extensions: 144
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 138
Number of HSP's successfully gapped: 2
Length of query: 358
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 272
Effective length of database: 1,212,660
Effective search space: 329843520
Effective search space used: 329843520
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 131 (55.1 bits)