BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0029.p (120 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l... 69 9e-14 >gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase large subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 69.3 bits (168), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 42/92 (45%), Positives = 51/92 (55%), Gaps = 1/92 (1%) Query: 1 MARTLYQKLVESHTVARLDEQNVLLYADLHIMNEYTSPQAFAGLHEAGRPVAVPGQHLSV 60 M +TLY KL ESH V D+ +LY D H+++E TSPQAF GL AGR +L V Sbjct: 1 MLKTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMV 60 Query: 61 VDHIIPTHPVSFRIIQDPSSALQASNLAKXAK 92 DH +PT + I DP S LQ L AK Sbjct: 61 ADHNVPTTDRAHG-IADPISRLQVETLDGNAK 91 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 398,367 Number of extensions: 14862 Number of successful extensions: 35 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 35 Number of HSP's successfully gapped: 1 Length of query: 120 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 46 Effective length of database: 1,269,480 Effective search space: 58396080 Effective search space used: 58396080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)