BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0027.p (145 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l... 100 7e-23 >gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase large subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 100 bits (248), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 50/102 (49%), Positives = 65/102 (63%) Query: 42 GAAFDVEHVFDANEVAPYVTWGTSPDQAVPVTGKVPAPASELDPVVRAASEQALRYTGLQ 101 GA FD+ +A E+ P VTWGTSP+ V V +VP P E DP R E+AL Y L+ Sbjct: 270 GAKFDMVVTLNAAEIKPQVTWGTSPEMVVAVDARVPDPDKEKDPTKRDGMEKALAYMALK 329 Query: 102 SGTNMEGIAVQRVFIGSCTNARIEDLRIVAGIVKGRKVAPSV 143 T +E I + +VFIGSCTN+RIEDLR A +V+G+ A +V Sbjct: 330 PNTPIEDIRIDKVFIGSCTNSRIEDLREAAKVVRGKFRASNV 371 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.131 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 472,042 Number of extensions: 19788 Number of successful extensions: 43 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 43 Number of HSP's successfully gapped: 1 Length of query: 145 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 68 Effective length of database: 1,255,275 Effective search space: 85358700 Effective search space used: 85358700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 126 (53.1 bits)