BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0021.p (644 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 1252 0.0 gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase ... 262 7e-71 gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase... 122 8e-29 gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-conta... 116 7e-27 gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family ... 114 3e-26 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 1252 bits (3239), Expect = 0.0, Method: Compositional matrix adjust. Identities = 588/644 (91%), Positives = 620/644 (96%), Gaps = 1/644 (0%) Query: 1 VSWEEALSHTASEFRRIQTKHGKDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCAR 60 VSWEEALS+ ASEFRRIQ KHGKDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCAR Sbjct: 315 VSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCAR 374 Query: 61 VCHSPTGYGLKQTLGESAGTQTFDSVMKSDVIMIIGANPASAHPVFASQMKRRLRQGAKL 120 VCHSPTGYGLKQTLGESAGTQTF+SVMKSDVI+I+GANPA+ HPVFASQ+KRR+RQGAKL Sbjct: 375 VCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGHPVFASQIKRRVRQGAKL 434 Query: 121 IVIDPRTTEMVKSAHIQADYHLKLRPGTNVAVITALAHVILSEGLQSEEYIKERCDVQSY 180 IVIDPRTTEMVKS HIQADYHLKLRPGTN A+ITALAHVILSEGLQ E YI+ERCD+QSY Sbjct: 435 IVIDPRTTEMVKSPHIQADYHLKLRPGTNTAIITALAHVILSEGLQDEAYIQERCDLQSY 494 Query: 181 EQWKEFVLRPDNSPEAMSAVCGVPAEELRGAARAFATGGNGAIYYGLGVTEHAQGSTMVI 240 + WKEFVLR DNSPEAM+A+ GVPAE +RGAAR +ATGGN AIYYGLGVTEHAQGST V+ Sbjct: 495 QDWKEFVLREDNSPEAMAAITGVPAEIIRGAARLYATGGNAAIYYGLGVTEHAQGSTTVM 554 Query: 241 GIANLAMATGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVRSQFEQAW 300 GIANLAM TGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVR+QFEQAW Sbjct: 555 GIANLAMCTGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVRNQFEQAW 614 Query: 301 GVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAAALQQMECIVVQDLF 360 GVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAAALQ MECIVVQDLF Sbjct: 615 GVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAAALQAMECIVVQDLF 674 Query: 361 LNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVRKVMPSKSGKSDWEVTVALAEALGYP 420 LNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVRKVMP K+GKSDWEVTVALAEALGYP Sbjct: 675 LNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVRKVMPPKAGKSDWEVTVALAEALGYP 734 Query: 421 MPYNHPSEIMDEIAALTPSFHGVSYDKLERLGSIQWPCNDAAPEGTPIMHIGSFIRGKGK 480 MPYNHPSEIMDEIAALTPSFHGVSYDKLE+LGSIQWPCN++APEGTPIMH+GSFIRGKGK Sbjct: 735 MPYNHPSEIMDEIAALTPSFHGVSYDKLEKLGSIQWPCNESAPEGTPIMHVGSFIRGKGK 794 Query: 481 FLNTQYFATDEKVTQRYPLILTTGRILSQYNVGAQTRRTENSQWHDEDRLEIHPHDAEDR 540 F+NTQYFATDEKVTQRYPLILTTGRILSQYNVGAQTRRTENSQWH EDRLEIHPHDAEDR Sbjct: 795 FINTQYFATDEKVTQRYPLILTTGRILSQYNVGAQTRRTENSQWHSEDRLEIHPHDAEDR 854 Query: 541 GVREGDWVGIESRAGQTVLRATVTDRVQPGVVYTTFHFPESGANVITTDNSDWATNCPEY 600 G+REGDWVG+ESRAGQTVLRATVT++VQPGVVYTTFHFPESGANVITTDNSDWATNCPEY Sbjct: 855 GIREGDWVGVESRAGQTVLRATVTEKVQPGVVYTTFHFPESGANVITTDNSDWATNCPEY 914 Query: 601 KVTAVQVMPVSQPSQWQQHYQEFHREQVRLLQGEKVSQEPLVTK 644 KVTAVQVMPV+QPSQWQQHYQ F+REQ+ LL+G+ EPLVTK Sbjct: 915 KVTAVQVMPVTQPSQWQQHYQRFNREQLDLLKGQ-AQAEPLVTK 957 >gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 922 Score = 262 bits (669), Expect = 7e-71, Method: Compositional matrix adjust. Identities = 180/643 (27%), Positives = 299/643 (46%), Gaps = 42/643 (6%) Query: 1 VSWEEALSHTASEFRRIQTKHGKDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCAR 60 ++W++AL H AS+F I +HG D++ S + E+ Y+ KL++ G+ N+DT +R Sbjct: 85 LAWDQALDHVASKFSAIIAEHGPDAVALYVSGQLLTEDYYIANKLMKGYIGSANIDTNSR 144 Query: 61 VCHSPTGYGLKQTLGESAGTQTFDSVMKSDVIMIIGANPASAHPVFASQMKR--RLRQGA 118 +C S G K+ GE ++ + +D+++++G+N A HP+ ++ R R Sbjct: 145 LCMSSAVAGHKRAFGEDLVPVCYEDLELADLVVLVGSNAAWCHPILFQRIARLKETRPAL 204 Query: 119 KLIVIDPRTTEMVKSAHIQADYHLKLRPGTNVAVITALAHVILSEGLQSEEYIKERCDVQ 178 +L+VIDPR T + AD HL ++PGT+V + L + GL+ +I+ + Sbjct: 205 RLVVIDPRRTATCE----LADLHLPIKPGTDVWLFNGLMSYLAHRGLRDNAFIEAH--TK 258 Query: 179 SYEQWKEFVLRPDNSPEAMSAVCGVPAEELRGAARAFATGGNGAIYYGLGVTEHAQGSTM 238 +++ P ++ +C + ++L AFA + +GV + + G+ Sbjct: 259 GFDEALAVADADCADPATVARLCKIDLKDLLAFYEAFANTEKVVTGFSMGVNQSSAGTDK 318 Query: 239 VIGIANLAMATGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVRSQFEQ 298 V I N +ATG +G+ G+G + GQ N G ++G + L + + R + Sbjct: 319 VNSIINCHLATGRIGKPGMGPFSITGQPNAMGGREVGGLANMLAAHMDLDQPLHRQIVQD 378 Query: 299 AW-GVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAAALQQMECIVVQ 357 W + +PGL+ +MF A +G K ++ + + S P+ V ALQ+ E +V Sbjct: 379 FWQSPRIADKPGLKAVDMFRAIEEGRIKAVWIMATNPMVSLPDADLVQRALQKCELVVSS 438 Query: 358 DLFL-NETAKYAHVFLPGSSFLEKDGTFTNAERRISRVRKVMPSKS-GKSDWEVTVALAE 415 D+ +T AHV LP ++ EKDGT TN+ERRISR R + + + DW + +A Sbjct: 439 DVITQTDTNALAHVLLPALAWGEKDGTVTNSERRISRQRAFLSAPGEARGDWRIICDVAR 498 Query: 416 ALGYP-MPYNHPSEIMDEIAALT------------------PSFHGVSYDKLERLGSIQW 456 +G+ + P EI DE A L+ G+ + L IQW Sbjct: 499 RMGFDGFDFAGPHEIFDEHARLSAHRNGEADASRVRRVFNLAGLTGMDRAAYDALAPIQW 558 Query: 457 PC---NDAAPEGTPIM---HIGSFIRGKGKFLNTQYFATDEKVTQRYPLILTTGRILSQY 510 P +D + GT + H S G+ +F+ T + + YPLIL TGR+ + Sbjct: 559 PVQRLSDGSVSGTARVLEDHRFSHADGRARFVPTAPRSAAHAACEDYPLILNTGRVRDHW 618 Query: 511 NVGAQTRRTENSQWH-DEDRLEIHPHDAEDRGVREGDWVGIESRAGQTVLRATVTDRVQP 569 + +T R+ H E ++IHP DA GVREG I SR G V R + Sbjct: 619 HTMTRTGRSATLASHIPESFIDIHPQDALLYGVREGGLARISSRWGAMVARVQHGGGIAR 678 Query: 570 GVVYTTFHFPESGAN-----VITTDNSDWATNCPEYKVTAVQV 607 G V+ H+ A+ + D + PE+K T V + Sbjct: 679 GSVFVPIHWNGQSASDARVGAVVNPVVDPVSGEPEFKHTPVAI 721 >gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 790 Score = 122 bits (306), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 160/670 (23%), Positives = 265/670 (39%), Gaps = 86/670 (12%) Query: 1 VSWEEALSHTASEFRRIQTKHGKDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCAR 60 VSW EA A++ R + K + S R + E +YL Q + R +G NN+ + Sbjct: 149 VSWSEAFDDIAAQLRAMDPK----KVVFYASGRASLETSYLYQLMARM-YGTNNLPDSSN 203 Query: 61 VCHSPTGYGLKQTLGESAGTQTFDSVMKSDVIMIIGANPASAHPVFASQMKRRLRQGAKL 120 +CH T L QT+G GT T D ++D I+ G N + P ++ ++G + Sbjct: 204 MCHESTSVALPQTIGVPVGTVTLDDFGQTDCILFFGHNTGTNAPRMLHPLEEVRKRGVPV 263 Query: 121 IVIDP-------------RTTEMVKSAHIQ-ADYHLKLRPGTNVAVITALAHVILSEGLQ 166 I +P EMV AH + +L+++ G + A +A IL + Sbjct: 264 ITFNPLRERGLERFVNPQSPKEMVTPAHTDISTQYLQIKIGGDSAAAIGMAKRILERDDK 323 Query: 167 S-EEYIKERCDVQSYEQ----WKEFVLRPDNSP-EAMSAVCGVPAEELRGAARAFATGGN 220 + +E + D+ E+ +++F +P + + GV EL AA +A G Sbjct: 324 ARQEGLPRLLDLAFIEEHTSGFEQFADAVRAAPWDELERHAGVSRAELELAADTYAGAGR 383 Query: 221 GAIYYGLGVTEHAQGSTMVIGIANLAMATGNVGREGVGVNPLRGQNNVQGSCDMGSFPHE 280 + YG+GVT+H + + + NL + GN+G+ G G+ P+RG +NVQG +G E Sbjct: 384 AMLMYGMGVTQHREAVRTIHMLTNLLLMRGNIGKPGAGICPIRGHSNVQGQRTVGI--TE 441 Query: 281 LPGYRHISDSTVRSQFEQAWGVTLNP--EPGLRIPNMFDAALDGSFKGLYCEGEDIVQSD 338 P + + +R+ + P E GL + DG + G + V++ Sbjct: 442 KPEL--VPNDKLRALYH------FEPPMEKGLNTVEACEKIRDGELSAFFMLGGNFVRAI 493 Query: 339 PNTQHVAAALQQMECIVVQDLFLNETAKY---AHVFLPGSSFLEKDGTFTNAERRI---- 391 P+ + AA +++ V N + LP +E D E+ + Sbjct: 494 PDHGVMEAAWRKLPLTVQVVTHFNRSCVIHGQTSYVLPCLGRIEIDRQ-RGGEQAVSVED 552 Query: 392 -------SRVRKVMPSKSGKSDWEVTVALAEALGYPMPYNHP----------SEIMDEIA 434 SR R S S+ + LA+ L +P+N + I D I Sbjct: 553 STGCMHGSRGRAEPASPHLLSEPAIVAELAKRL---LPFNPQVDWDGWVGDYARIRDAIE 609 Query: 435 ALTPSFHGVSYDKLERLGSIQWPCNDAAPE---GTPIMHIGSFIRGKGKFLNTQYFATDE 491 A P ++ G P AA E TP G+ F+ F D Sbjct: 610 ATYPDIFRDFNARMWNPGGFARPL--AARERQWKTP--------NGRANFMTPDGFDPDP 659 Query: 492 KVTQRYP--LILTTGRILSQYNVGAQTRRTE-NSQWHDEDRLEIHPHDAEDRGVREGDWV 548 + P L L T R SQ+N + W L ++ D + G+ GD+V Sbjct: 660 DMPGDAPEVLRLMTTRGDSQFNTTVYSLDDRFRGVWGTRRVLLMNLRDLQQHGLAAGDFV 719 Query: 549 GIESRAGQTVLRATVTDRVQP---GVVYTTFHFPESGANVITTDNSDWATNCPEYKVTAV 605 E+ + V R RV+P V +FPE +I + + P K + Sbjct: 720 CAETVSADGVERRVPHLRVEPFDIPVGCVMGYFPELN-RLIPLFHHARGSKVPAAKSVPI 778 Query: 606 QVMP-VSQPS 614 +++P + +PS Sbjct: 779 RLIPELREPS 788 >gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-containing) formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 775 Score = 116 bits (290), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 90/375 (24%), Positives = 162/375 (43%), Gaps = 32/375 (8%) Query: 1 VSWEEALSHTASEFRRIQTKHGKDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCAR 60 + W +A + A + D S R +NE +L Q VR +G NN C+ Sbjct: 128 IEWADAFALMAKHLNALPDPDMADFY---VSGRASNEAAFLFQLFVRQ-YGTNNFPDCSN 183 Query: 61 VCHSPTGYGLKQTLGESAGTQTFDSVMKSDVIMIIGANPASAHPVFASQMKRRLRQGAKL 120 +CH PT GL T+G GT + D +++ G NPA+ HP +++ ++GA + Sbjct: 184 MCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMMGELRHASKRGATI 243 Query: 121 IVIDP-RTTEMVKSAHIQADYHLKLRPGTNVAVI---------TALAHVILSEGLQSEEY 170 + I+P + + + A Q+ + T ++ + AL ++ ++ ++ Sbjct: 244 VAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLGGDLALIKGVIKRTIELDDA 303 Query: 171 IK----ERC-DVQSYEQ----WKEFVLRPD-NSPEAMSAVCGVPAEELRGAARAFATGGN 220 + ER DV+ E+ ++EF + S + + A GV E++ A+ + G Sbjct: 304 ARAAGAERVIDVKFIEEHTAGFEEFAAQARAESWDDIVAESGVSREDIEKLAQVYVKGKA 363 Query: 221 GAIYYGLGVTEHAQGSTMVIGIANLAMATGNVGREGVGVNPLRGQNNVQGSCDMGSFPHE 280 +G+G+T+H + ++N+ M GN+GR+G G+ P+RG +NVQG +G Sbjct: 364 VIATWGMGLTQHKHAVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGIDEKP 423 Query: 281 LPGYRHISDSTVRSQFEQAWGVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPN 340 P + + EQ +G G A L+G K G + + P+ Sbjct: 424 TPAF--------LDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPD 475 Query: 341 TQHVAAALQQMECIV 355 T A++ V Sbjct: 476 TPRTFEAMRSCNLTV 490 >gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 114 bits (285), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 147/597 (24%), Positives = 241/597 (40%), Gaps = 94/597 (15%) Query: 61 VCHSPTGYGLKQTLGESAGTQTFDSVMKSDVIMIIGANPASAHPVFASQMKRRLRQGAKL 120 +C S G G + T+G G + V + +I+I G NP +++ F ++++ R GA+L Sbjct: 138 ICASAGGAGYRYTIGARVGAD-LEQVDHAKLILIWGGNPIASNLHFWTRVQTAKRNGARL 196 Query: 121 IVIDPRTTEMVKSAHIQADYHLKLRPGTNVAVITALAHVILSEGLQSEEYIKERCDVQSY 180 I IDP + + H H+ L PGT+ A+ + HV++SE L +YI + Sbjct: 197 IAIDPYRSLTAEKCH----QHIALLPGTDAALALGMMHVLISEDLLDHDYIARH--TLGF 250 Query: 181 EQWKEFVLRPDNSPEAMSAVCGVPAEELRGAARAF---ATGGNGAIY---YGLGVTEHAQ 234 EQ +E V + +P + VCG+ AEE+ AR + A G G YGL + + Sbjct: 251 EQLRERV--AEWTPARTAEVCGISAEEVLNLAREYGRVALAGEGVFIRANYGL---QRVR 305 Query: 235 GSTMVIGIANLAMATGNVG--REGVGVNPLRGQNNVQGSCDMGSFPHELPGY----RHIS 288 G M N+A VG R G + ++ S P G+ R I+ Sbjct: 306 GGGM--AARNVACLPALVGAWRHPAGGVQMSVSDSFPKDAAALSRPDLAAGHGKPARVIN 363 Query: 289 DSTVRSQFEQAWGVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAAAL 348 ST+ P+ + L + V P + VA Sbjct: 364 MSTIGDALLHPASADFGPK----------------VEALIVYNSNPVAVAPESAKVAQGF 407 Query: 349 QQMECI-VVQDLFLNETAKYAHVFLPGSSFLEKDGTFTNAERR--ISRVRKVMPSKSGKS 405 + + VV + F +TA YA + LP ++ LE + ++ + P K Sbjct: 408 AREDLFTVVLEHFQTDTADYADILLPATTQLEHIDVHASYGHVYIMANNAAIAPMGEAKP 467 Query: 406 DWEVTVALAEALGYPMPYNHPSEIMDEIAALT-----PSFHGVSYDKLERLG--SIQWPC 458 + E+ LA A+G+ S+ D++AA+ P G +++L++ G + P Sbjct: 468 NTEIFRRLAAAMGFTEDCFQDSD--DQLAAVAFKRDDPRSAGYDWEQLKQKGWQRLAVPV 525 Query: 459 NDAAPEG--TPIMHIGSFIRGKGKFLNTQY-----------------FATDEKVTQRYPL 499 A G TP GK +F + Q A++ ++ RYPL Sbjct: 526 APFAEGGFATP--------SGKCEFYSEQMKAAGLDPLPAYIPPYESVASNPQLAARYPL 577 Query: 500 ILTTG---RILSQYNVGAQTRRTENSQWHDEDRLEIHPHDAEDRGVREGDWVGIESRAGQ 556 + + L+ V Q+ R + H L++HP DA RG+ +GD V I + G Sbjct: 578 AMISPPARNFLNSSFVNVQSLRDTEGEPH----LDMHPQDAAARGISQGDMVRIFNDRGS 633 Query: 557 TVLRATVTDRVQPGVV------YTTFHFPESGANVITTDNSDWATNCPEYKVTAVQV 607 R VTD+ + G+V + F AN +T+ + P + VQV Sbjct: 634 LQARVRVTDKARVGLVVGLSIWWKKFAADGKNANEVTSQRLTDMGHAPTFYDVLVQV 690 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,072,537 Number of extensions: 88318 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 214 Number of HSP's successfully gapped: 5 Length of query: 644 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 553 Effective length of database: 1,188,985 Effective search space: 657508705 Effective search space used: 657508705 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)