BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0004.p (107 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 149 4e-38 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 61 2e-11 gi|300313658|ref|YP_003777750.1| transport transmembrane protein... 59 1e-10 gi|300309906|ref|YP_003773998.1| major facilitator superfamily p... 55 2e-09 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 54 3e-09 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 149 bits (377), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 70/80 (87%), Positives = 75/80 (93%) Query: 14 AKENIKPSHVRYLILFMLFIVTTVNYADRATLSIAGTSMKAELGLDPVMMGYIFSAFSWA 73 A E KP+HVRYLILF+LF+VTTVNYADRATLSIAG++MK EL LDPVMMGYIFSAFSWA Sbjct: 2 AGEVAKPTHVRYLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWA 61 Query: 74 YVLAQLPGGWLLDRFGSKKI 93 YVLAQLPGGWLLDRFGSKKI Sbjct: 62 YVLAQLPGGWLLDRFGSKKI 81 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 60.8 bits (146), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 32/69 (46%), Positives = 44/69 (63%) Query: 23 VRYLILFMLFIVTTVNYADRATLSIAGTSMKAELGLDPVMMGYIFSAFSWAYVLAQLPGG 82 ++ + L L + VNY DR+TLSIA S+ EL L MG + SAFS AY +QLP G Sbjct: 14 IQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPIG 73 Query: 83 WLLDRFGSK 91 +LD+FG++ Sbjct: 74 AMLDKFGAR 82 >gi|300313658|ref|YP_003777750.1| transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 58.5 bits (140), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 26/57 (45%), Positives = 37/57 (64%) Query: 37 VNYADRATLSIAGTSMKAELGLDPVMMGYIFSAFSWAYVLAQLPGGWLLDRFGSKKI 93 V+Y DRA ++ A T + +E L P +G + S+F Y L QLPGGWL DR+GS+ + Sbjct: 24 VSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLGYSLLQLPGGWLADRYGSRPV 80 >gi|300309906|ref|YP_003773998.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 54.7 bits (130), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 28/63 (44%), Positives = 36/63 (57%) Query: 31 LFIVTTVNYADRATLSIAGTSMKAELGLDPVMMGYIFSAFSWAYVLAQLPGGWLLDRFGS 90 LF+ V YADR +S A + E LD G + SAF +Y L QL GGWL DR+GS Sbjct: 17 LFLAWAVGYADRIVMSTAIIPISKEFALDAQQAGMVLSAFYVSYALMQLMGGWLSDRYGS 76 Query: 91 KKI 93 + + Sbjct: 77 RIV 79 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 53.5 bits (127), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 30/83 (36%), Positives = 47/83 (56%) Query: 11 SASAKENIKPSHVRYLILFMLFIVTTVNYADRATLSIAGTSMKAELGLDPVMMGYIFSAF 70 +A+ + K +H R+++ ++FIV TV ADRA L +A ++ E + G + S F Sbjct: 3 AAAIQHTKKKTHFRWVVGALIFIVYTVAAADRANLGVALPFLRKEFVMSNTEAGALASIF 62 Query: 71 SWAYVLAQLPGGWLLDRFGSKKI 93 +AY LAQLP G RFG ++ Sbjct: 63 LFAYSLAQLPSGLAYTRFGIPRV 85 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 295,783 Number of extensions: 10001 Number of successful extensions: 99 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 6 Length of query: 107 Length of database: 1,619,870 Length adjustment: 72 Effective length of query: 35 Effective length of database: 1,278,950 Effective search space: 44763250 Effective search space used: 44763250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 124 (52.4 bits)