BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0002.p (174 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310196|ref|YP_003774288.1| ATP-dependent DNA helicase [Her... 121 2e-29 >gi|300310196|ref|YP_003774288.1| ATP-dependent DNA helicase [Herbaspirillum seropedicae SmR1] Length = 1158 Score = 121 bits (304), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 61/72 (84%), Positives = 63/72 (87%) Query: 6 AIAAYEINGRPAEAAAFTAAACDPRQSVVVEACAGSGKTWLLVARMLRLLLAGAQPAELL 65 A AAYE+NG +AA FT AACDPR SVVVEACAGSGKTWLLVARMLRLLLAGAQ ELL Sbjct: 7 APAAYEVNGEAVDAAVFTRAACDPRHSVVVEACAGSGKTWLLVARMLRLLLAGAQAPELL 66 Query: 66 AITFTRKAAQEM 77 AITFTRKAAQEM Sbjct: 67 AITFTRKAAQEM 78 Score = 68.2 bits (165), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 34/65 (52%), Positives = 45/65 (69%) Query: 104 RDARLTVWTDEALKARLYEISSLLGKGTATNQKRAVAIETLVSGEASAEQFVALASQFHD 163 RDARL++W + AL AR +++ LLG+G + NQ RAVAIE+ +S + E F AL SQF D Sbjct: 254 RDARLSLWEEHALVARASQLAILLGQGGSANQARAVAIESALSAGPALEHFDALYSQFFD 313 Query: 164 DSGKP 168 D KP Sbjct: 314 DKDKP 318 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.128 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 354,799 Number of extensions: 8856 Number of successful extensions: 55 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 57 Number of HSP's successfully gapped: 2 Length of query: 174 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 95 Effective length of database: 1,245,805 Effective search space: 118351475 Effective search space used: 118351475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)