BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0055.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313358|ref|YP_003777450.1| multidrug ABC transporter perme... 62 3e-11 >gi|300313358|ref|YP_003777450.1| multidrug ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 254 Score = 62.4 bits (150), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 59/238 (24%), Positives = 106/238 (44%), Gaps = 13/238 (5%) Query: 14 FYPIWRRNFLVWRKLMAASIIGNIAEPLITLIAFGYGLGSILKEIDGIPYIHYLASGSIC 73 F ++ + L + K+ +I I L+ L+ FG+ L ++ G+ Y +L G + Sbjct: 4 FRTLFYKEILRFWKVATQTITAPIVTALLYLLIFGHVLQDRVQVYPGVNYTGFLVPGLVM 63 Query: 74 MSVMTAASFEALYSAYSRMSVQRTWDALMNAPLALDDIVLAELLWSATKSLFSGVAILCV 133 MSV+ A F S+ + + ++ PL+ ++ +L + + L G + V Sbjct: 64 MSVLQNA-FANSSSSLIQSKITGNLVFVLLTPLSHWELFGGYVLAAVVRGLMVGAGVFVV 122 Query: 134 MFALGIGLHPQVLLVPPVLFL------AGMTFGALGLITNALAKGYDFFTYYFTLVLTPM 187 G + V P+ L AG+ G +GLI A+ +D + ++ P+ Sbjct: 123 TVWFG-----HLDFVAPLWILVFALLGAGI-LGTMGLIAGIWAEKFDQLAAFQNFLIVPL 176 Query: 188 TFLSGVYYPVAQLPSWLQTVSQFLPLSAAVNLVRPLVLGGWPQAPLLDLAVLAFYCCA 245 TFL+GV+Y + LP + Q VS F P ++ R G AP+ L ++A + A Sbjct: 177 TFLAGVFYSIHSLPPFWQAVSHFNPFFYMIDGFRYGFFGQSDIAPMTSLGIVAVFFVA 234 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.143 0.452 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 763,480 Number of extensions: 29560 Number of successful extensions: 113 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 112 Number of HSP's successfully gapped: 1 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 129 (54.3 bits)