BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0003.p (235 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_766666.1| dehydrogenase [Bradyrhizobium japonicum USDA 11... 474 e-132 ref|NP_962511.1| FabG3_2 [Mycobacterium avium subsp. paratubercu... 270 1e-70 ref|ZP_06849754.1| 3-alpha-(or 20-beta)-hydroxysteroid dehydroge... 270 1e-70 ref|YP_004524862.1| 20-beta-hydroxysteroid dehydrogenase FabG3 [... 268 6e-70 ref|YP_887819.1| 3-alpha-(or 20-beta)-hydroxysteroid dehydrogena... 268 7e-70 >ref|NP_766666.1| dehydrogenase [Bradyrhizobium japonicum USDA 110] dbj|BAC45291.1| bll0026 [Bradyrhizobium japonicum USDA 110] Length = 252 Score = 474 bits (1219), Expect = e-132, Method: Compositional matrix adjust. Identities = 235/235 (100%), Positives = 235/235 (100%) Query: 1 MGAAHARLLVAEGAKVVITDILDNEGATFAAELGPSAIYVRQDVAQPGEWCQAIAAAEGK 60 MGAAHARLLVAEGAKVVITDILDNEGATFAAELGPSAIYVRQDVAQPGEWCQAIAAAEGK Sbjct: 18 MGAAHARLLVAEGAKVVITDILDNEGATFAAELGPSAIYVRQDVAQPGEWCQAIAAAEGK 77 Query: 61 FGAVHGLVNNAGIASMAPIERFPLEQWEKTLAVNLTGVFLGMQTALPAMRRAGGGSIVNI 120 FGAVHGLVNNAGIASMAPIERFPLEQWEKTLAVNLTGVFLGMQTALPAMRRAGGGSIVNI Sbjct: 78 FGAVHGLVNNAGIASMAPIERFPLEQWEKTLAVNLTGVFLGMQTALPAMRRAGGGSIVNI 137 Query: 121 SSVEGMRGSPGLHAYVASKFGVRGITKSAALEAAASGVRINSIHPGFINTRMTENIDLSS 180 SSVEGMRGSPGLHAYVASKFGVRGITKSAALEAAASGVRINSIHPGFINTRMTENIDLSS Sbjct: 138 SSVEGMRGSPGLHAYVASKFGVRGITKSAALEAAASGVRINSIHPGFINTRMTENIDLSS 197 Query: 181 FPIPLARPAEPQEVSQAVLFLLSDESSYLTGSEIVVDGGLTIGVPQKGQAVENIF 235 FPIPLARPAEPQEVSQAVLFLLSDESSYLTGSEIVVDGGLTIGVPQKGQAVENIF Sbjct: 198 FPIPLARPAEPQEVSQAVLFLLSDESSYLTGSEIVVDGGLTIGVPQKGQAVENIF 252 >ref|NP_962511.1| FabG3_2 [Mycobacterium avium subsp. paratuberculosis K-10] ref|YP_884253.1| 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase [Mycobacterium avium 104] ref|ZP_05218994.1| 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase [Mycobacterium avium subsp. avium ATCC 25291] gb|AAS06127.1| FabG3_2 [Mycobacterium avium subsp. paratuberculosis K-10] gb|ABK64766.1| 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase [Mycobacterium avium 104] gb|EGO38797.1| dehydrogenase of unknown specificity, short-chain alcohol dehydrogenase like protein [Mycobacterium avium subsp. paratuberculosis S397] Length = 246 Score = 270 bits (691), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 134/227 (59%), Positives = 166/227 (73%) Query: 1 MGAAHARLLVAEGAKVVITDILDNEGATFAAELGPSAIYVRQDVAQPGEWCQAIAAAEGK 60 MGA HARLL AEGAKVVI DILD+EG A E+G S YV DV QP +W A+ A G+ Sbjct: 18 MGAEHARLLAAEGAKVVIGDILDDEGKAVADEIGDSVRYVHLDVTQPDQWDAAVETAVGE 77 Query: 61 FGAVHGLVNNAGIASMAPIERFPLEQWEKTLAVNLTGVFLGMQTALPAMRRAGGGSIVNI 120 FG ++ LVNNAG ++ P++ F L +W+K + VNLTG FLGM+ A+ M AGGGSI+NI Sbjct: 78 FGKLNVLVNNAGTVALGPLKSFDLAKWQKVIDVNLTGTFLGMRVAVEPMIAAGGGSIINI 137 Query: 121 SSVEGMRGSPGLHAYVASKFGVRGITKSAALEAAASGVRINSIHPGFINTRMTENIDLSS 180 SS+EG+RG+P +H YVASK+GVRG+ KSAALE A +R+NS+HPGFI T MT+++ Sbjct: 138 SSIEGLRGAPMVHPYVASKWGVRGLAKSAALELAPHNIRVNSVHPGFIRTPMTKHLPDDM 197 Query: 181 FPIPLARPAEPQEVSQAVLFLLSDESSYLTGSEIVVDGGLTIGVPQK 227 +PL RPAE +EVS VLFL SDESSY TGSE V+DGGL VP K Sbjct: 198 VTVPLGRPAESREVSTFVLFLASDESSYATGSEFVMDGGLVTDVPHK 244 >ref|ZP_06849754.1| 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase [Mycobacterium parascrofulaceum ATCC BAA-614] gb|EFG76900.1| 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase [Mycobacterium parascrofulaceum ATCC BAA-614] Length = 246 Score = 270 bits (690), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 134/227 (59%), Positives = 165/227 (72%) Query: 1 MGAAHARLLVAEGAKVVITDILDNEGATFAAELGPSAIYVRQDVAQPGEWCQAIAAAEGK 60 MGA HARLLV+EGAKVVI DILD+EG A E+G S YV DV QP +W A+ A G+ Sbjct: 18 MGAEHARLLVSEGAKVVIGDILDDEGKAVADEIGDSVRYVHLDVTQPDQWDAAVETAVGE 77 Query: 61 FGAVHGLVNNAGIASMAPIERFPLEQWEKTLAVNLTGVFLGMQTALPAMRRAGGGSIVNI 120 FG ++ LVNNAG ++ P+ F L +W+K + VNLTG FLGM+ A+ M AGGGSI+NI Sbjct: 78 FGKLNVLVNNAGTVALGPLRSFDLAKWQKVIDVNLTGTFLGMRVAVDPMIAAGGGSIINI 137 Query: 121 SSVEGMRGSPGLHAYVASKFGVRGITKSAALEAAASGVRINSIHPGFINTRMTENIDLSS 180 SS+EG+RG+P +H YVASK+ VRG+ KSAALE A +R+NS+HPGFI T MT+++ Sbjct: 138 SSIEGLRGAPMVHPYVASKWAVRGLAKSAALELAPHNIRVNSVHPGFIRTPMTQHLPDDM 197 Query: 181 FPIPLARPAEPQEVSQAVLFLLSDESSYLTGSEIVVDGGLTIGVPQK 227 IPL RPAE +EVS VLFL SDESSY TGSE V+DGGL VP K Sbjct: 198 VTIPLGRPAESREVSTFVLFLASDESSYATGSEFVMDGGLVTDVPHK 244 >ref|YP_004524862.1| 20-beta-hydroxysteroid dehydrogenase FabG3 [Mycobacterium sp. JDM601] gb|AEF37608.1| 20-beta-hydroxysteroid dehydrogenase FabG3 [Mycobacterium sp. JDM601] Length = 229 Score = 268 bits (684), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 131/227 (57%), Positives = 165/227 (72%) Query: 1 MGAAHARLLVAEGAKVVITDILDNEGATFAAELGPSAIYVRQDVAQPGEWCQAIAAAEGK 60 MGA+HAR+LVAEGAKVVI D+LD EGA AAELG +A YV DV P +W A+ AA Sbjct: 1 MGASHARMLVAEGAKVVIGDVLDEEGAALAAELGDAAAYVHLDVRDPEQWAAAVTAAVDG 60 Query: 61 FGAVHGLVNNAGIASMAPIERFPLEQWEKTLAVNLTGVFLGMQTALPAMRRAGGGSIVNI 120 FG ++ LVNNAG ++ + +F L+QW + VNL G FLGM+ A+ +M AGGGSI+N+ Sbjct: 61 FGKLNVLVNNAGTVALGSLRKFDLDQWHNVIDVNLNGTFLGMRAAIDSMIAAGGGSIINV 120 Query: 121 SSVEGMRGSPGLHAYVASKFGVRGITKSAALEAAASGVRINSIHPGFINTRMTENIDLSS 180 SS+EG+RG+P +H YVASK+ VRG++KSAALE A +R+NS+HPGFI T MTE++ Sbjct: 121 SSIEGLRGAPMVHPYVASKWAVRGLSKSAALELARHNIRVNSLHPGFIRTPMTEHLPEDM 180 Query: 181 FPIPLARPAEPQEVSQAVLFLLSDESSYLTGSEIVVDGGLTIGVPQK 227 IPL RP E +EVS V+FL SDESSY TGSE V+DGGL VP K Sbjct: 181 VTIPLGRPGESEEVSTFVVFLASDESSYATGSEFVMDGGLVTDVPHK 227 >ref|YP_887819.1| 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase [Mycobacterium smegmatis str. MC2 155] gb|ABK75595.1| 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase [Mycobacterium smegmatis str. MC2 155] Length = 245 Score = 268 bits (684), Expect = 7e-70, Method: Compositional matrix adjust. Identities = 134/227 (59%), Positives = 165/227 (72%) Query: 1 MGAAHARLLVAEGAKVVITDILDNEGATFAAELGPSAIYVRQDVAQPGEWCQAIAAAEGK 60 MGAAHAR LVAEGAKVVI DILD+EGA AAELG +A YV DV Q +W A+A A Sbjct: 18 MGAAHARALVAEGAKVVIGDILDDEGAALAAELGDAARYVHLDVTQAEDWDTAVATATTD 77 Query: 61 FGAVHGLVNNAGIASMAPIERFPLEQWEKTLAVNLTGVFLGMQTALPAMRRAGGGSIVNI 120 FG ++ L+NNAGI ++ I +F + +W+ + VNLTG FLGMQ ++ AM+ AGGGSI+N+ Sbjct: 78 FGLLNVLINNAGIVALGAIGKFDMTKWQNVIDVNLTGTFLGMQASVGAMKAAGGGSIINV 137 Query: 121 SSVEGMRGSPGLHAYVASKFGVRGITKSAALEAAASGVRINSIHPGFINTRMTENIDLSS 180 SS+EG+RG+P +H YVASK+ VRG+TKSAALE A +R+NSIHPGFI T MTE+ Sbjct: 138 SSIEGLRGAPMVHPYVASKWAVRGLTKSAALELGAHQIRVNSIHPGFIRTPMTEHFPDDM 197 Query: 181 FPIPLARPAEPQEVSQAVLFLLSDESSYLTGSEIVVDGGLTIGVPQK 227 IPL RP E EVS V+FL SDES Y TG+E V+DGGL VP K Sbjct: 198 LRIPLGRPGEVDEVSSFVVFLASDESRYATGAEFVMDGGLVNDVPHK 244