BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0259.0020.p (348 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|ABC26868.1| HypE [Bradyrhizobium sp. UPM1167] 652 0.0 ref|NP_773567.1| HypE protein [Bradyrhizobium japonicum USDA 110... 586 e-165 ref|YP_001203814.1| hydrogenase maturation protein HypE [Bradyrh... 578 e-163 ref|YP_001238108.1| hydrogenase maturation [Bradyrhizobium sp. B... 577 e-162 ref|NP_768377.1| hydrogenase expression/formation protein [Brady... 568 e-160 >gb|ABC26868.1| HypE [Bradyrhizobium sp. UPM1167] Length = 348 Score = 652 bits (1683), Expect = 0.0, Method: Compositional matrix adjust. Identities = 323/348 (92%), Positives = 334/348 (95%) Query: 1 MSVKDYHGKLDVENGRVDLSHGSGGRAMAQLISGLFHQAFGNEWLARCNDQSAFEVAAGR 60 MS K Y GKLDV+NGRVDLSHGSGGRAMAQLIS +FHQAFGNEWLARCNDQ+AF+VAAGR Sbjct: 1 MSAKAYQGKLDVDNGRVDLSHGSGGRAMAQLISCIFHQAFGNEWLARCNDQAAFDVAAGR 60 Query: 61 MVMTTDGYVVSPLFFPGGNIGSLAVHGTINDVVMAGARPLYLSASFIIEEGFRFSDLRTI 120 MVMTTDGYVVSPLFFPGGNIGSLAVHGTINDVVMAGARPLYLSASFIIEEGFRFSDLRTI Sbjct: 61 MVMTTDGYVVSPLFFPGGNIGSLAVHGTINDVVMAGARPLYLSASFIIEEGFRFSDLRTI 120 Query: 121 AQSMGAAARGAGVYIITGDTKVVERGKADGLFISTTGIGVLADGLDLSADKARIGDRVLV 180 AQSMG AAR AGVYIITGDTKVVERGKADGLFISTTGIG LADGLDLSADKARIGDRVL+ Sbjct: 121 AQSMGEAARAAGVYIITGDTKVVERGKADGLFISTTGIGFLADGLDLSADKARIGDRVLM 180 Query: 181 SGSLGDHGVAIMSTRQNAALQVEIVSDSASLHDLVAVMVAAGGRGIHVMRDPTRGGLAAT 240 SGSLGDHGVAIMS RQNAALQVEI+SDSASLHDLVAVM+ AGGRGI VMRDPTRGGLAA Sbjct: 181 SGSLGDHGVAIMSARQNAALQVEIISDSASLHDLVAVMMTAGGRGIRVMRDPTRGGLAAA 240 Query: 241 LNEIAHQSGLGFRLQEAAIPVKPAVAATCELLGLDPIHVANEGKLVAIVAPGVADAVLAA 300 LNEIAHQSGLGFRLQEAAIP+KPAVAA CELLGLDPIHVANEGKLVAIVAP VADAVLAA Sbjct: 241 LNEIAHQSGLGFRLQEAAIPIKPAVAAACELLGLDPIHVANEGKLVAIVAPDVADAVLAA 300 Query: 301 MKGHPLGCDAADIGEAVADDHKFVQVATSFGGGRIVDWLSGEQLPRIC 348 MK HPLGCDAADIGEAVAD+H+FVQ+ATSFGGGRIVDWLSGEQLPRIC Sbjct: 301 MKAHPLGCDAADIGEAVADEHEFVQMATSFGGGRIVDWLSGEQLPRIC 348 >ref|NP_773567.1| HypE protein [Bradyrhizobium japonicum USDA 110] sp|P31906.3|HYPE_BRAJA RecName: Full=Hydrogenase expression/formation protein hypE dbj|BAC52192.1| HypE protein [Bradyrhizobium japonicum USDA 110] Length = 346 Score = 586 bits (1510), Expect = e-165, Method: Compositional matrix adjust. Identities = 291/346 (84%), Positives = 313/346 (90%) Query: 3 VKDYHGKLDVENGRVDLSHGSGGRAMAQLISGLFHQAFGNEWLARCNDQSAFEVAAGRMV 62 +K Y KLD+ NG VDLSHGSGGRAMAQLISGLFH+AFGNEWLAR NDQSAF+V AGRMV Sbjct: 1 MKAYQRKLDIRNGCVDLSHGSGGRAMAQLISGLFHEAFGNEWLARGNDQSAFDVRAGRMV 60 Query: 63 MTTDGYVVSPLFFPGGNIGSLAVHGTINDVVMAGARPLYLSASFIIEEGFRFSDLRTIAQ 122 MTTDGYVVSPLFFPGGNIGSLAVHGT+ND+ MAGA+PLYLSASFIIEEGFRF+DL+ IA+ Sbjct: 61 MTTDGYVVSPLFFPGGNIGSLAVHGTVNDIAMAGAKPLYLSASFIIEEGFRFADLKLIAE 120 Query: 123 SMGAAARGAGVYIITGDTKVVERGKADGLFISTTGIGVLADGLDLSADKARIGDRVLVSG 182 SMGAAAR A V+IITGDTKVVERGKADGLFIST G+GV+ DGLDLSA+KAR+GDRVL+SG Sbjct: 121 SMGAAAREADVHIITGDTKVVERGKADGLFISTAGVGVVPDGLDLSAEKARVGDRVLISG 180 Query: 183 SLGDHGVAIMSTRQNAALQVEIVSDSASLHDLVAVMVAAGGRGIHVMRDPTRGGLAATLN 242 +LGDHGVAIMS RQN A + EIVSDSASLHDLVA MV AGGRGI +MRDPTRGGLAATLN Sbjct: 181 TLGDHGVAIMSKRQNLAFETEIVSDSASLHDLVARMVQAGGRGIRLMRDPTRGGLAATLN 240 Query: 243 EIAHQSGLGFRLQEAAIPVKPAVAATCELLGLDPIHVANEGKLVAIVAPGVADAVLAAMK 302 EIA QS LGF L E AIPVKP VAA CELLGLDP+HVANEGKLVAIVAP A AVLAAM+ Sbjct: 241 EIAQQSNLGFHLLEEAIPVKPGVAAACELLGLDPLHVANEGKLVAIVAPEAAGAVLAAMR 300 Query: 303 GHPLGCDAADIGEAVADDHKFVQVATSFGGGRIVDWLSGEQLPRIC 348 HPLG DAADIGEAVADDH FVQ+ATSFGGGRIVDWLSGEQLPRIC Sbjct: 301 AHPLGRDAADIGEAVADDHHFVQMATSFGGGRIVDWLSGEQLPRIC 346 >ref|YP_001203814.1| hydrogenase maturation protein HypE [Bradyrhizobium sp. ORS 278] emb|CAL75577.1| hydrogenase maturation protein HypE [Bradyrhizobium sp. ORS 278] Length = 346 Score = 578 bits (1490), Expect = e-163, Method: Compositional matrix adjust. Identities = 285/343 (83%), Positives = 313/343 (91%) Query: 6 YHGKLDVENGRVDLSHGSGGRAMAQLISGLFHQAFGNEWLARCNDQSAFEVAAGRMVMTT 65 + +LD++NG VDLSHG+GGRAMAQLI+GLFH+AFGNE+LAR NDQSAF+VAAGRMVMTT Sbjct: 4 HQRRLDLKNGCVDLSHGAGGRAMAQLIAGLFHEAFGNEFLARGNDQSAFDVAAGRMVMTT 63 Query: 66 DGYVVSPLFFPGGNIGSLAVHGTINDVVMAGARPLYLSASFIIEEGFRFSDLRTIAQSMG 125 DGYVVSPLFFPGGNIGSLAVHGT+ND+ MAGA+PLYLSASFIIEEGFRF DL+ IA +MG Sbjct: 64 DGYVVSPLFFPGGNIGSLAVHGTVNDIAMAGAKPLYLSASFIIEEGFRFDDLKRIADAMG 123 Query: 126 AAARGAGVYIITGDTKVVERGKADGLFISTTGIGVLADGLDLSADKARIGDRVLVSGSLG 185 AAAR AG+ IITGDTKVVERGKADG+FIST G+GVL DGLDLSADKAR GDRVL+SGSLG Sbjct: 124 AAAREAGIAIITGDTKVVERGKADGVFISTAGVGVLPDGLDLSADKARPGDRVLLSGSLG 183 Query: 186 DHGVAIMSTRQNAALQVEIVSDSASLHDLVAVMVAAGGRGIHVMRDPTRGGLAATLNEIA 245 DHGVAIMS RQN EIVSDSASLH LVA MV AGG+GI +MRDPTRGGLAAT+NEIA Sbjct: 184 DHGVAIMSKRQNLTFDTEIVSDSASLHGLVAAMVVAGGQGIRLMRDPTRGGLAATMNEIA 243 Query: 246 HQSGLGFRLQEAAIPVKPAVAATCELLGLDPIHVANEGKLVAIVAPGVADAVLAAMKGHP 305 QS LGFRLQE +IPVKPAVAA CELLGLDP+HVANEGKLVA+VAP VA+AVLAAMK HP Sbjct: 244 QQSNLGFRLQEESIPVKPAVAAACELLGLDPLHVANEGKLVAVVAPDVANAVLAAMKAHP 303 Query: 306 LGCDAADIGEAVADDHKFVQVATSFGGGRIVDWLSGEQLPRIC 348 LG +AADIGEAVADDH+FVQ+ATSFGGGRIVDWLSGEQLPRIC Sbjct: 304 LGGEAADIGEAVADDHRFVQMATSFGGGRIVDWLSGEQLPRIC 346 >ref|YP_001238108.1| hydrogenase maturation [Bradyrhizobium sp. BTAi1] gb|ABQ34202.1| hydrogenase maturation [Bradyrhizobium sp. BTAi1] Length = 346 Score = 577 bits (1486), Expect = e-162, Method: Compositional matrix adjust. Identities = 286/343 (83%), Positives = 309/343 (90%) Query: 6 YHGKLDVENGRVDLSHGSGGRAMAQLISGLFHQAFGNEWLARCNDQSAFEVAAGRMVMTT 65 + +LD++ G VDLSHG+GGRAMAQLI+GLFH+AFGNEWLAR NDQSAF+VA GRMVMTT Sbjct: 4 HQRRLDLKTGCVDLSHGAGGRAMAQLIAGLFHEAFGNEWLARGNDQSAFDVATGRMVMTT 63 Query: 66 DGYVVSPLFFPGGNIGSLAVHGTINDVVMAGARPLYLSASFIIEEGFRFSDLRTIAQSMG 125 DGYVVSPLFFPGGNIGSLAVHGT+ND+ MAGARPLYLSASFIIEEGFRFSDL+ IA SMG Sbjct: 64 DGYVVSPLFFPGGNIGSLAVHGTVNDIAMAGARPLYLSASFIIEEGFRFSDLKRIADSMG 123 Query: 126 AAARGAGVYIITGDTKVVERGKADGLFISTTGIGVLADGLDLSADKARIGDRVLVSGSLG 185 AAR AGV IITGDTKVVERGKADG+FIST G+GVL DGLDLSADKAR GDRVL+SGSLG Sbjct: 124 EAAREAGVAIITGDTKVVERGKADGVFISTAGVGVLPDGLDLSADKARPGDRVLLSGSLG 183 Query: 186 DHGVAIMSTRQNAALQVEIVSDSASLHDLVAVMVAAGGRGIHVMRDPTRGGLAATLNEIA 245 DHGVAIMS RQN EIVSDSA+LH LVA MVAAGG+G+ +MRDPTRGGLAAT+NEIA Sbjct: 184 DHGVAIMSKRQNLTFDTEIVSDSAALHGLVAAMVAAGGQGLRLMRDPTRGGLAATMNEIA 243 Query: 246 HQSGLGFRLQEAAIPVKPAVAATCELLGLDPIHVANEGKLVAIVAPGVADAVLAAMKGHP 305 QS LGFRLQE AIPVKPAVAA CELLGLDP+HVANEGKLVA+VAP AD VLAAMK HP Sbjct: 244 QQSNLGFRLQEDAIPVKPAVAAACELLGLDPLHVANEGKLVAVVAPEQADTVLAAMKAHP 303 Query: 306 LGCDAADIGEAVADDHKFVQVATSFGGGRIVDWLSGEQLPRIC 348 LG DAA IGEAVADDH+FVQ+ATSFGGGRIVDWLSGEQLPRIC Sbjct: 304 LGRDAAQIGEAVADDHRFVQMATSFGGGRIVDWLSGEQLPRIC 346 >ref|NP_768377.1| hydrogenase expression/formation protein [Bradyrhizobium japonicum USDA 110] gb|AAG60723.1|AF322012_28 HypE [Bradyrhizobium japonicum] dbj|BAC47002.1| hydrogenase expression/formation protein [Bradyrhizobium japonicum USDA 110] Length = 348 Score = 568 bits (1464), Expect = e-160, Method: Compositional matrix adjust. Identities = 297/348 (85%), Positives = 315/348 (90%) Query: 1 MSVKDYHGKLDVENGRVDLSHGSGGRAMAQLISGLFHQAFGNEWLARCNDQSAFEVAAGR 60 MSVK Y KLDVENGRV+LSHG+GGRAMAQLISGLFHQAFGNEWLA NDQSAF+V AGR Sbjct: 1 MSVKAYQRKLDVENGRVELSHGAGGRAMAQLISGLFHQAFGNEWLAGSNDQSAFDVTAGR 60 Query: 61 MVMTTDGYVVSPLFFPGGNIGSLAVHGTINDVVMAGARPLYLSASFIIEEGFRFSDLRTI 120 MVMTTDGYV+SPLFFPGGNIGSLAVHGTIND+ M+GARPLYLSASFIIEEGFRFSDL+TI Sbjct: 61 MVMTTDGYVISPLFFPGGNIGSLAVHGTINDIAMSGARPLYLSASFIIEEGFRFSDLKTI 120 Query: 121 AQSMGAAARGAGVYIITGDTKVVERGKADGLFISTTGIGVLADGLDLSADKARIGDRVLV 180 A+SMGAAA AGVYII GDTKVVERGKADGLFIS TGIG++ADGLDLSADKA++GDRVL+ Sbjct: 121 AESMGAAACAAGVYIIAGDTKVVERGKADGLFISMTGIGLVADGLDLSADKAKVGDRVLL 180 Query: 181 SGSLGDHGVAIMSTRQNAALQVEIVSDSASLHDLVAVMVAAGGRGIHVMRDPTRGGLAAT 240 SGSLGDHGVAIMS RQN A Q EIVSDSA+LH L A MVAAGG GI VMRDPTRGGLAA Sbjct: 181 SGSLGDHGVAIMSKRQNVAFQTEIVSDSAALHSLAAAMVAAGGGGIRVMRDPTRGGLAAA 240 Query: 241 LNEIAHQSGLGFRLQEAAIPVKPAVAATCELLGLDPIHVANEGKLVAIVAPGVADAVLAA 300 LNEIA QS LGF LQE+ IPVKPAVAATCELLGL+PIHVANEGKLVAIVAP ADAVLAA Sbjct: 241 LNEIALQSELGFHLQESMIPVKPAVAATCELLGLEPIHVANEGKLVAIVAPDSADAVLAA 300 Query: 301 MKGHPLGCDAADIGEAVADDHKFVQVATSFGGGRIVDWLSGEQLPRIC 348 MK HPLG +AADIGEAVADDHKFVQ+ T FGG RIVDWL GEQLPRIC Sbjct: 301 MKAHPLGSNAADIGEAVADDHKFVQMTTDFGGRRIVDWLWGEQLPRIC 348