BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0259.0016.p (243 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|ABC26864.1| HypB [Bradyrhizobium sp. UPM1167] 415 e-114 ref|NP_773571.1| HypB protein [Bradyrhizobium japonicum USDA 110... 377 e-103 gb|AAA17763.1| HypB [Bradyrhizobium japonicum] 375 e-102 gb|ABC17838.1| HypB [Bradyrhizobium sp. UPM1029] 368 e-100 ref|YP_001238104.1| hydrogenase nickel incorporation [Bradyrhizo... 363 1e-98 >gb|ABC26864.1| HypB [Bradyrhizobium sp. UPM1167] Length = 319 Score = 415 bits (1066), Expect = e-114, Method: Compositional matrix adjust. Identities = 205/239 (85%), Positives = 217/239 (90%) Query: 5 ADPAALKVAGKSCEHIIQVGRDILGKNNRIAAENRALFMAHELLVFNLMSSPGAGKTSLL 64 A PA LKVA E ++Q+ RDIL KN+RIAA+NRALF+A ELLVFNL+SSPGAGKTSLL Sbjct: 81 AGPAGLKVADMRRERVVQIERDILSKNSRIAADNRALFVADELLVFNLVSSPGAGKTSLL 140 Query: 65 VRAVSELKRSWTIGVIEGDQQTSNDAERIRSAGVPAIQVNTGKCCHLDAAMIGEAYRRLP 124 VRAVSELKRS IGVIEGDQQTSNDAERIR+ GVPAIQVNTGK CHLDAAMIGEAYRRLP Sbjct: 141 VRAVSELKRSRPIGVIEGDQQTSNDAERIRATGVPAIQVNTGKGCHLDAAMIGEAYRRLP 200 Query: 125 PLTRGILFIENVGNLVCPAAFDLGEACKIVVLSTTEGEDKPLKYPDMFAASSLMLINKID 184 PL GILFIENVGNLVCPAAFDLGEACKIVVLSTTEGEDKPLKYPD+F ASSLMLINK+D Sbjct: 201 PLIGGILFIENVGNLVCPAAFDLGEACKIVVLSTTEGEDKPLKYPDIFGASSLMLINKVD 260 Query: 185 LASLLEFDLGRTVEYARRVNPKIEVLTVSARTGEGFGSLYGWIDEQVAHQRRALEDTRR 243 LA LLEFDLG+TVEYARRVNPKIEVLTVSARTGEGFG LY WID+Q AH RRALEDTRR Sbjct: 261 LAPLLEFDLGKTVEYARRVNPKIEVLTVSARTGEGFGGLYAWIDKQAAHHRRALEDTRR 319 >ref|NP_773571.1| HypB protein [Bradyrhizobium japonicum USDA 110] sp|Q45257.2|HYPB_BRAJA RecName: Full=Hydrogenase nickel incorporation protein hypB dbj|BAC52196.1| HypB protein [Bradyrhizobium japonicum USDA 110] Length = 302 Score = 377 bits (968), Expect = e-103, Method: Compositional matrix adjust. Identities = 184/224 (82%), Positives = 201/224 (89%) Query: 4 GADPAALKVAGKSCEHIIQVGRDILGKNNRIAAENRALFMAHELLVFNLMSSPGAGKTSL 63 GA+PA K+AG S + IIQV RDILGKN+R+AA+NRA F A E+L FNL+SSPGAGKTSL Sbjct: 63 GANPAGQKIAGMSSDRIIQVERDILGKNDRLAADNRARFRADEVLAFNLVSSPGAGKTSL 122 Query: 64 LVRAVSELKRSWTIGVIEGDQQTSNDAERIRSAGVPAIQVNTGKCCHLDAAMIGEAYRRL 123 LVRAVSELK S+ IGVIEGDQQTSNDAERIR+ GVPAIQVNTGK CHLDAAM+GEAY RL Sbjct: 123 LVRAVSELKDSFAIGVIEGDQQTSNDAERIRATGVPAIQVNTGKGCHLDAAMVGEAYDRL 182 Query: 124 PPLTRGILFIENVGNLVCPAAFDLGEACKIVVLSTTEGEDKPLKYPDMFAASSLMLINKI 183 P L G+LFIENVGNLVCPAAFDLGEACKIVV STTEGEDKPLKYPDMFAASSLMLINKI Sbjct: 183 PWLNGGLLFIENVGNLVCPAAFDLGEACKIVVFSTTEGEDKPLKYPDMFAASSLMLINKI 242 Query: 184 DLASLLEFDLGRTVEYARRVNPKIEVLTVSARTGEGFGSLYGWI 227 DLAS+L+FDL RT+EYARRVNPKIEVLT+SARTGEGF + Y WI Sbjct: 243 DLASVLDFDLARTIEYARRVNPKIEVLTLSARTGEGFAAFYAWI 286 >gb|AAA17763.1| HypB [Bradyrhizobium japonicum] Length = 302 Score = 375 bits (963), Expect = e-102, Method: Compositional matrix adjust. Identities = 183/224 (81%), Positives = 200/224 (89%) Query: 4 GADPAALKVAGKSCEHIIQVGRDILGKNNRIAAENRALFMAHELLVFNLMSSPGAGKTSL 63 GA+PA K+ G S + IIQV RDILGKN+R+AA+NRA F A E+L FNL+SSPGAGKTSL Sbjct: 63 GANPAGQKITGMSSDRIIQVERDILGKNDRLAADNRARFRADEVLAFNLVSSPGAGKTSL 122 Query: 64 LVRAVSELKRSWTIGVIEGDQQTSNDAERIRSAGVPAIQVNTGKCCHLDAAMIGEAYRRL 123 LVRAVSELK S+ IGVIEGDQQTSNDAERIR+ GVPAIQVNTGK CHLDAAM+GEAY RL Sbjct: 123 LVRAVSELKDSFAIGVIEGDQQTSNDAERIRATGVPAIQVNTGKGCHLDAAMVGEAYDRL 182 Query: 124 PPLTRGILFIENVGNLVCPAAFDLGEACKIVVLSTTEGEDKPLKYPDMFAASSLMLINKI 183 P L G+LFIENVGNLVCPAAFDLGEACKIVV STTEGEDKPLKYPDMFAASSLMLINKI Sbjct: 183 PWLNGGLLFIENVGNLVCPAAFDLGEACKIVVFSTTEGEDKPLKYPDMFAASSLMLINKI 242 Query: 184 DLASLLEFDLGRTVEYARRVNPKIEVLTVSARTGEGFGSLYGWI 227 DLAS+L+FDL RT+EYARRVNPKIEVLT+SARTGEGF + Y WI Sbjct: 243 DLASVLDFDLARTIEYARRVNPKIEVLTLSARTGEGFAAFYAWI 286 >gb|ABC17838.1| HypB [Bradyrhizobium sp. UPM1029] Length = 306 Score = 368 bits (944), Expect = e-100, Method: Compositional matrix adjust. Identities = 179/229 (78%), Positives = 201/229 (87%) Query: 4 GADPAALKVAGKSCEHIIQVGRDILGKNNRIAAENRALFMAHELLVFNLMSSPGAGKTSL 63 GA+PA K+AG S + IIQV RDIL KN+R+AA+NRA F A +L FNL+SSPG GKTSL Sbjct: 67 GANPAGQKIAGMSSDRIIQVERDILDKNDRLAADNRARFAADGVLAFNLVSSPGTGKTSL 126 Query: 64 LVRAVSELKRSWTIGVIEGDQQTSNDAERIRSAGVPAIQVNTGKCCHLDAAMIGEAYRRL 123 LVRAVSELK S+ IGVIEGDQQTSNDAERIR+ GVPAIQVNTGK CHLDAAM+GEAY RL Sbjct: 127 LVRAVSELKASFAIGVIEGDQQTSNDAERIRATGVPAIQVNTGKGCHLDAAMVGEAYDRL 186 Query: 124 PPLTRGILFIENVGNLVCPAAFDLGEACKIVVLSTTEGEDKPLKYPDMFAASSLMLINKI 183 P L G+LFIENVGNLVCPAAFDLGEACKIVV STTEGEDKPLKYPDMFAASSLMLINKI Sbjct: 187 PWLNGGLLFIENVGNLVCPAAFDLGEACKIVVFSTTEGEDKPLKYPDMFAASSLMLINKI 246 Query: 184 DLASLLEFDLGRTVEYARRVNPKIEVLTVSARTGEGFGSLYGWIDEQVA 232 DLA +L+FDL +T++YARRVNPKIEVLTVSARTGEGF + + WI +++A Sbjct: 247 DLAPVLDFDLAKTIDYARRVNPKIEVLTVSARTGEGFAAFFAWIRKRMA 295 >ref|YP_001238104.1| hydrogenase nickel incorporation [Bradyrhizobium sp. BTAi1] gb|ABQ34198.1| Hydrogenase nickel incorporation [Bradyrhizobium sp. BTAi1] Length = 321 Score = 363 bits (931), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 173/231 (74%), Positives = 200/231 (86%) Query: 5 ADPAALKVAGKSCEHIIQVGRDILGKNNRIAAENRALFMAHELLVFNLMSSPGAGKTSLL 64 A+PA ++AG S IIQ+ RDILGKN+R+AAENRA F AH++L FN++SSPGAGKTSLL Sbjct: 82 ANPAGQEIAGLSSGRIIQIERDILGKNDRLAAENRARFAAHDVLAFNVVSSPGAGKTSLL 141 Query: 65 VRAVSELKRSWTIGVIEGDQQTSNDAERIRSAGVPAIQVNTGKCCHLDAAMIGEAYRRLP 124 VRAVS+LK S + VIEGDQQTSNDAERIR+ GVPAIQ+NTGK CHLDAAM+GEAYRRLP Sbjct: 142 VRAVSDLKPSRPVAVIEGDQQTSNDAERIRATGVPAIQINTGKGCHLDAAMVGEAYRRLP 201 Query: 125 PLTRGILFIENVGNLVCPAAFDLGEACKIVVLSTTEGEDKPLKYPDMFAASSLMLINKID 184 + G+LFIENVGNLVCPAAFDLGEACKIVV STTEGEDKPLKYPDMFAAS+LMLINKID Sbjct: 202 SMRGGLLFIENVGNLVCPAAFDLGEACKIVVFSTTEGEDKPLKYPDMFAASALMLINKID 261 Query: 185 LASLLEFDLGRTVEYARRVNPKIEVLTVSARTGEGFGSLYGWIDEQVAHQR 235 LA +L+FDL +T+EYARRVNP+IEVLT+SA+TGEG + Y WI +Q R Sbjct: 262 LAPVLDFDLAQTIEYARRVNPRIEVLTISAKTGEGVSAFYAWIRKQTDRMR 312