BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0259.0004.p (366 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|ABC26854.1| HupS [Bradyrhizobium sp. UPM1167] 683 0.0 ref|YP_001203798.1| uptake hydrogenase small subunit [Bradyrhizo... 640 0.0 ref|YP_780163.1| hydrogenase (NiFe) small subunit HydA [Rhodopse... 639 0.0 ref|NP_773582.1| uptake hydrogenase [Bradyrhizobium japonicum US... 637 0.0 gb|ABC17827.1| HupS [Bradyrhizobium sp. UPM1029] 637 0.0 >gb|ABC26854.1| HupS [Bradyrhizobium sp. UPM1167] Length = 366 Score = 683 bits (1762), Expect = 0.0, Method: Compositional matrix adjust. Identities = 331/366 (90%), Positives = 347/366 (94%) Query: 1 MSGATETFYSVIRRQGITRRSFHKFCSLTASSFGLGPLAASRIANALETKPRMPVIWLHG 60 M ETFYSVIRRQGITRRSFHKFCSLTA+SFGLGPLAAS IANALETKPR+PVIWLHG Sbjct: 1 MGRIEETFYSVIRRQGITRRSFHKFCSLTAASFGLGPLAASSIANALETKPRIPVIWLHG 60 Query: 61 LECTCCSESFIRSAHPLIKDVLLSMISLDYDDMIMAAAGHQAEAILEEARLRYKGRYVLA 120 LECTCCSESFIRSAHPLIKDVLLSMISLDYDDM+MAAAGHQAEA+L+E+R RY G+YVLA Sbjct: 61 LECTCCSESFIRSAHPLIKDVLLSMISLDYDDMVMAAAGHQAEAVLQESRARYNGKYVLA 120 Query: 121 VEGNPPLNEGGMFCIDGGKPFVERLRWMAESSMAVIAWGTCASWGCVQAAKPNPTGAVPI 180 VEGN PLNEGG+FCIDGGKPFVERLRWMAE+SMAVIAWGTCASWGCVQAA+PNPTG VPI Sbjct: 121 VEGNAPLNEGGVFCIDGGKPFVERLRWMAEASMAVIAWGTCASWGCVQAARPNPTGTVPI 180 Query: 181 DKVITDKPIVKVPGCPPIAEVMTGLVTFITTFGRLPELDRQGRPKMFYSERIHDKCYRRS 240 DKVI++KPIVKVPGCPPIAE MTGLVTFITTFGR PELDRQGRPKMFYS+RIHDKCYRRS Sbjct: 181 DKVISNKPIVKVPGCPPIAEAMTGLVTFITTFGRPPELDRQGRPKMFYSQRIHDKCYRRS 240 Query: 241 HFDAGQFVEAWDDECARKGYCLYKMGCKGPTTYNACSALRWNGGVSFPIQSGHGCFGCSE 300 HFDAGQFVE WDDECARKGYCLYK+GCKGPTTYNACS LRWNGGVSFPIQSGHGC CSE Sbjct: 241 HFDAGQFVEEWDDECARKGYCLYKVGCKGPTTYNACSTLRWNGGVSFPIQSGHGCLSCSE 300 Query: 301 DGFWDKGSFYDRLTTIQQFGIEKNADQIGMAAAGAVGLTVAAHAAITAVKRLTHKPDRAV 360 DGFWDKGSFYDRLT IQQFGIEKNADQIGMAAAGAVGLTVAAHAA+TAVKRLT KPDRA Sbjct: 301 DGFWDKGSFYDRLTAIQQFGIEKNADQIGMAAAGAVGLTVAAHAAVTAVKRLTRKPDRAA 360 Query: 361 RKDKPT 366 K+KPT Sbjct: 361 HKNKPT 366 >ref|YP_001203798.1| uptake hydrogenase small subunit [Bradyrhizobium sp. ORS 278] emb|CAL75561.1| Uptake hydrogenase small subunit precursor (Hydrogenlyase) (Membrane-bound hydrogenase small subunit) [Bradyrhizobium sp. ORS 278] Length = 365 Score = 640 bits (1651), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/362 (85%), Positives = 333/362 (91%) Query: 1 MSGATETFYSVIRRQGITRRSFHKFCSLTASSFGLGPLAASRIANALETKPRMPVIWLHG 60 M TETFYSVIRRQGITRRSFHKFCSLTA+S GLGPLA++RIANALETKPR PVIW+HG Sbjct: 1 MGATTETFYSVIRRQGITRRSFHKFCSLTATSLGLGPLASARIANALETKPRTPVIWMHG 60 Query: 61 LECTCCSESFIRSAHPLIKDVLLSMISLDYDDMIMAAAGHQAEAILEEARLRYKGRYVLA 120 LECTCCSESFIRSAHPL+KD +LSMISLDYDD IMAAAG QAE ILEE R +YKG+Y+LA Sbjct: 61 LECTCCSESFIRSAHPLVKDAVLSMISLDYDDTIMAAAGQQAEEILEETRAKYKGQYILA 120 Query: 121 VEGNPPLNEGGMFCIDGGKPFVERLRWMAESSMAVIAWGTCASWGCVQAAKPNPTGAVPI 180 VEGNPPLNEGGMFCIDGGKPFVE+L+ MAE +MA+IAWG CASWGCVQAAKPNPT A PI Sbjct: 121 VEGNPPLNEGGMFCIDGGKPFVEKLKVMAEDAMAIIAWGACASWGCVQAAKPNPTQATPI 180 Query: 181 DKVITDKPIVKVPGCPPIAEVMTGLVTFITTFGRLPELDRQGRPKMFYSERIHDKCYRRS 240 DKVIT+KPI+KVPGCPPIAEVMTG+VTFITTFG+LPELDRQGRPKMFYS+RIHDKCYRR Sbjct: 181 DKVITNKPIIKVPGCPPIAEVMTGVVTFITTFGKLPELDRQGRPKMFYSQRIHDKCYRRP 240 Query: 241 HFDAGQFVEAWDDECARKGYCLYKMGCKGPTTYNACSALRWNGGVSFPIQSGHGCFGCSE 300 HFDAGQFVE WDDE ARKGYCLYKMGCKGPTTYNACS +RWNGGVSFPIQSGHGC GCSE Sbjct: 241 HFDAGQFVEEWDDEAARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSE 300 Query: 301 DGFWDKGSFYDRLTTIQQFGIEKNADQIGMAAAGAVGLTVAAHAAITAVKRLTHKPDRAV 360 DGFWDKGSFYDRLTTI+QFGIE+NAD++GMAAAG VG+ VAAHAA+TAVKRL K D A Sbjct: 301 DGFWDKGSFYDRLTTIKQFGIERNADEVGMAAAGTVGVAVAAHAAVTAVKRLATKRDDAS 360 Query: 361 RK 362 K Sbjct: 361 AK 362 >ref|YP_780163.1| hydrogenase (NiFe) small subunit HydA [Rhodopseudomonas palustris BisA53] gb|ABJ05183.1| hydrogenase (NiFe) small subunit HydA [Rhodopseudomonas palustris BisA53] Length = 371 Score = 639 bits (1647), Expect = 0.0, Method: Compositional matrix adjust. Identities = 292/357 (81%), Positives = 323/357 (90%) Query: 1 MSGATETFYSVIRRQGITRRSFHKFCSLTASSFGLGPLAASRIANALETKPRMPVIWLHG 60 M ATETFY VIRRQGITRRSF KFCSLTA+S GLGP+ A+ IA ALETKPR+PVIW+HG Sbjct: 1 MGAATETFYEVIRRQGITRRSFIKFCSLTATSLGLGPIGAAEIAQALETKPRVPVIWMHG 60 Query: 61 LECTCCSESFIRSAHPLIKDVLLSMISLDYDDMIMAAAGHQAEAILEEARLRYKGRYVLA 120 LECTCCSESFIRSAHPL+KD +LSMISLDYDD IMAAAGHQAEAILEE R +YKG+Y+LA Sbjct: 61 LECTCCSESFIRSAHPLVKDAVLSMISLDYDDTIMAAAGHQAEAILEETRKKYKGQYILA 120 Query: 121 VEGNPPLNEGGMFCIDGGKPFVERLRWMAESSMAVIAWGTCASWGCVQAAKPNPTGAVPI 180 VEGNPPLNE GM+CIDGG+PFVE+L+ MA+ SMAVIAWG CASWGCVQAAKPNPT AVPI Sbjct: 121 VEGNPPLNEDGMYCIDGGRPFVEKLKEMADDSMAVIAWGACASWGCVQAAKPNPTQAVPI 180 Query: 181 DKVITDKPIVKVPGCPPIAEVMTGLVTFITTFGRLPELDRQGRPKMFYSERIHDKCYRRS 240 DKVI +KPI+KVPGCPPIAEVMTG+VT+ITTFG+LP+LDRQGRPKMFYS+RIHDKCYRR Sbjct: 181 DKVIKNKPIIKVPGCPPIAEVMTGVVTYITTFGKLPDLDRQGRPKMFYSQRIHDKCYRRP 240 Query: 241 HFDAGQFVEAWDDECARKGYCLYKMGCKGPTTYNACSALRWNGGVSFPIQSGHGCFGCSE 300 HFDAGQFVE WDD+ ARKG+CLYKMGCKGPTTYNACS +RWNGGVSFPIQSGHGC GCSE Sbjct: 241 HFDAGQFVEEWDDDAARKGFCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSE 300 Query: 301 DGFWDKGSFYDRLTTIQQFGIEKNADQIGMAAAGAVGLTVAAHAAITAVKRLTHKPD 357 DGFWDKG FYDRLTTIQQFGIE NAD+IG AG VG+ +AAHAA+T V+ LT K + Sbjct: 301 DGFWDKGGFYDRLTTIQQFGIEANADKIGATVAGTVGVAIAAHAAVTTVRNLTRKKE 357 >ref|NP_773582.1| uptake hydrogenase [Bradyrhizobium japonicum USDA 110] sp|P12635.1|MBHS_BRAJA RecName: Full=Uptake hydrogenase small subunit; AltName: Full=Hydrogenlyase; AltName: Full=Membrane-bound hydrogenase small subunit; Flags: Precursor gb|AAA26218.1| uptake hydrogenase (hupS) precursor [Bradyrhizobium japonicum] dbj|BAC52207.1| uptake hydrogenase precursor [Bradyrhizobium japonicum USDA 110] Length = 363 Score = 637 bits (1643), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/359 (86%), Positives = 334/359 (93%) Query: 1 MSGATETFYSVIRRQGITRRSFHKFCSLTASSFGLGPLAASRIANALETKPRMPVIWLHG 60 M ATETFYSVIRRQGITRRSFHKFCSLTA+S GLGPLAASRIANALETKPR+PVIW+HG Sbjct: 1 MGAATETFYSVIRRQGITRRSFHKFCSLTATSLGLGPLAASRIANALETKPRVPVIWMHG 60 Query: 61 LECTCCSESFIRSAHPLIKDVLLSMISLDYDDMIMAAAGHQAEAILEEARLRYKGRYVLA 120 LECTCCSESFIRSAHPL+KD +LSMISLDYDD IMAAAGHQAEAILEE R ++KG+Y+LA Sbjct: 61 LECTCCSESFIRSAHPLVKDAVLSMISLDYDDTIMAAAGHQAEAILEETRAKHKGQYILA 120 Query: 121 VEGNPPLNEGGMFCIDGGKPFVERLRWMAESSMAVIAWGTCASWGCVQAAKPNPTGAVPI 180 VEGNPPLNEGGMFCIDGGKPFVE+L+ MAE +MA+IAWG CASWGCVQAAKPNPT A PI Sbjct: 121 VEGNPPLNEGGMFCIDGGKPFVEKLKMMAEDAMAIIAWGACASWGCVQAAKPNPTQATPI 180 Query: 181 DKVITDKPIVKVPGCPPIAEVMTGLVTFITTFGRLPELDRQGRPKMFYSERIHDKCYRRS 240 DKVIT+KPI+KVPGCPPIAEVMTG+VTFITTFG+LPELDRQGRPKMFYS+RIHDKCYRR Sbjct: 181 DKVITNKPIIKVPGCPPIAEVMTGVVTFITTFGKLPELDRQGRPKMFYSQRIHDKCYRRP 240 Query: 241 HFDAGQFVEAWDDECARKGYCLYKMGCKGPTTYNACSALRWNGGVSFPIQSGHGCFGCSE 300 HFDAGQFVE WDDE ARKGYCLYKMGCKGPTTYNACS +RWNGGVSFPIQSGHGC GCSE Sbjct: 241 HFDAGQFVEEWDDEAARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSE 300 Query: 301 DGFWDKGSFYDRLTTIQQFGIEKNADQIGMAAAGAVGLTVAAHAAITAVKRLTHKPDRA 359 DGFWDKGSFYDRLT I+QFGIEKNADQIGM AAGAVG VAAHAA+TAVKRL K + A Sbjct: 301 DGFWDKGSFYDRLTNIKQFGIEKNADQIGMVAAGAVGAAVAAHAAVTAVKRLATKREDA 359 >gb|ABC17827.1| HupS [Bradyrhizobium sp. UPM1029] Length = 363 Score = 637 bits (1643), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/359 (86%), Positives = 334/359 (93%) Query: 1 MSGATETFYSVIRRQGITRRSFHKFCSLTASSFGLGPLAASRIANALETKPRMPVIWLHG 60 M ATETFYSVIRRQGITRRSFHKFCSLT +S GLGPLAASRIANALETKPR+PVIW+HG Sbjct: 1 MGAATETFYSVIRRQGITRRSFHKFCSLTGTSLGLGPLAASRIANALETKPRVPVIWMHG 60 Query: 61 LECTCCSESFIRSAHPLIKDVLLSMISLDYDDMIMAAAGHQAEAILEEARLRYKGRYVLA 120 LECTCCSESFIRSAHPL+KDV+LSMISLDYDD IMAAAGHQAEAILEE R ++KG+Y+LA Sbjct: 61 LECTCCSESFIRSAHPLVKDVVLSMISLDYDDTIMAAAGHQAEAILEETRAKHKGQYILA 120 Query: 121 VEGNPPLNEGGMFCIDGGKPFVERLRWMAESSMAVIAWGTCASWGCVQAAKPNPTGAVPI 180 VEGNPPLNEGGMFCIDGGKPFVE+L+ MAE +MA+IAWG CASWGCVQAAKPNPT A PI Sbjct: 121 VEGNPPLNEGGMFCIDGGKPFVEKLKMMAEDAMAIIAWGACASWGCVQAAKPNPTQATPI 180 Query: 181 DKVITDKPIVKVPGCPPIAEVMTGLVTFITTFGRLPELDRQGRPKMFYSERIHDKCYRRS 240 DKVIT+KPI+KVPGCPPIAEVMTG+VTFITTFG+LPELDRQGRPKMFYS+RIHDKCYRR Sbjct: 181 DKVITNKPIIKVPGCPPIAEVMTGVVTFITTFGKLPELDRQGRPKMFYSQRIHDKCYRRP 240 Query: 241 HFDAGQFVEAWDDECARKGYCLYKMGCKGPTTYNACSALRWNGGVSFPIQSGHGCFGCSE 300 HFDAGQFVE WDDE ARKGYCLYKMGCKGPTTYNACS +RWNGGVSFPIQSGHGC GCSE Sbjct: 241 HFDAGQFVEEWDDEAARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSE 300 Query: 301 DGFWDKGSFYDRLTTIQQFGIEKNADQIGMAAAGAVGLTVAAHAAITAVKRLTHKPDRA 359 DGFWDKGSFYDRLT I+QFGIEKNADQIGM AAGAVG VAAHAA+TAVKRL K + A Sbjct: 301 DGFWDKGSFYDRLTNIKQFGIEKNADQIGMVAAGAVGAAVAAHAAVTAVKRLATKREDA 359