BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0258.0012.p (237 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774438.1| nitroreductase [Bradyrhizobium japonicum USDA 1... 414 e-114 ref|YP_570727.1| nitroreductase [Rhodopseudomonas palustris BisB... 335 2e-90 ref|YP_485309.1| nitroreductase [Rhodopseudomonas palustris HaA2... 332 3e-89 ref|YP_782728.1| nitroreductase [Rhodopseudomonas palustris BisA... 314 8e-84 ref|YP_001242903.1| hypothetical protein BBta_7115 [Bradyrhizobi... 312 3e-83 >ref|NP_774438.1| nitroreductase [Bradyrhizobium japonicum USDA 110] dbj|BAC53063.1| bll7798 [Bradyrhizobium japonicum USDA 110] Length = 232 Score = 414 bits (1065), Expect = e-114, Method: Compositional matrix adjust. Identities = 197/236 (83%), Positives = 211/236 (89%), Gaps = 5/236 (2%) Query: 1 MDAKTPNPRYATEDRIGALEELLNERYSVRAFQPREVPREVIEHILSTAQRTASWCNSQP 60 MDAK +RIG LEELLNERYSVRAF P+EV R IEH+L+TAQRTASWCNSQP Sbjct: 1 MDAKVNQ-----NNRIGVLEELLNERYSVRAFLPKEVDRATIEHVLTTAQRTASWCNSQP 55 Query: 61 WQVLIVSGEAKERFRKAIYAEASRGLAEDYDFTPPREYVGVYLERRRESGFQLYNTLGIA 120 WQV+I SGEAKERFR+ IY EAS GL +DYDFTPPREYVGVYL+RRRESGFQLYNTLGI Sbjct: 56 WQVIIASGEAKERFRRLIYKEASSGLGDDYDFTPPREYVGVYLDRRRESGFQLYNTLGIP 115 Query: 121 RGDKAAYAKQALENYNFFGAPHIAVIHTNEPLGIYGAIDCGGYVSNFMLAAQALGLGTIP 180 RGD++ YA+QALENYNFFGAPH+AVIHTNEPLGIYGAIDCG YVSNFMLAAQALGLGTIP Sbjct: 116 RGDRSGYARQALENYNFFGAPHVAVIHTNEPLGIYGAIDCGAYVSNFMLAAQALGLGTIP 175 Query: 181 QAAIARHSGLIRRHFNLADDRRVVCGISFGYADHAQKVNSYRTSRATVEDTVTFVD 236 QAA+ARHSGLIRRHFNL DDRRVVCGISFGYADHA KVNSYRTSRA+V DTVTFVD Sbjct: 176 QAALARHSGLIRRHFNLPDDRRVVCGISFGYADHAHKVNSYRTSRASVADTVTFVD 231 >ref|YP_570727.1| nitroreductase [Rhodopseudomonas palustris BisB5] gb|ABE40826.1| nitroreductase [Rhodopseudomonas palustris BisB5] Length = 232 Score = 335 bits (860), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 158/226 (69%), Positives = 182/226 (80%) Query: 11 ATEDRIGALEELLNERYSVRAFQPREVPREVIEHILSTAQRTASWCNSQPWQVLIVSGEA 70 AT I LEEL+ RYS RAF+P VPRE I+ +L+ AQ+TASWCNSQPWQVLI SGEA Sbjct: 6 ATTAPIDVLEELMAARYSCRAFRPDPVPRETIDRMLAAAQKTASWCNSQPWQVLITSGEA 65 Query: 71 KERFRKAIYAEASRGLAEDYDFTPPREYVGVYLERRRESGFQLYNTLGIARGDKAAYAKQ 130 +FR IY A+ G DF PREY GVYLERRRESGFQLYNTLGIA+GDK AYAKQ Sbjct: 66 TRKFRDVIYNVAASGAPASGDFEFPREYRGVYLERRRESGFQLYNTLGIAKGDKMAYAKQ 125 Query: 131 ALENYNFFGAPHIAVIHTNEPLGIYGAIDCGGYVSNFMLAAQALGLGTIPQAAIARHSGL 190 ALEN+NFFGAPH+A+I T+E LG+YGAIDCGGYV++ MLAAQALGL T+PQAA+A HSG+ Sbjct: 126 ALENFNFFGAPHVAIITTDEALGVYGAIDCGGYVTSLMLAAQALGLATVPQAALAFHSGV 185 Query: 191 IRRHFNLADDRRVVCGISFGYADHAQKVNSYRTSRATVEDTVTFVD 236 +R+HF L DDRRVVCGISFGYAD A K NSYRTSRA + + TFV+ Sbjct: 186 VRKHFGLGDDRRVVCGISFGYADRAHKANSYRTSRAALGEVATFVE 231 >ref|YP_485309.1| nitroreductase [Rhodopseudomonas palustris HaA2] gb|ABD06398.1| Nitroreductase [Rhodopseudomonas palustris HaA2] Length = 230 Score = 332 bits (850), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 154/221 (69%), Positives = 179/221 (80%) Query: 16 IGALEELLNERYSVRAFQPREVPREVIEHILSTAQRTASWCNSQPWQVLIVSGEAKERFR 75 I LEEL+ RYS RAF+P VPR+ IE +L+ AQ+TASWCNSQPWQVLI SGEA +RFR Sbjct: 10 INVLEELMAARYSCRAFKPDPVPRDTIERVLAAAQKTASWCNSQPWQVLITSGEATKRFR 69 Query: 76 KAIYAEASRGLAEDYDFTPPREYVGVYLERRRESGFQLYNTLGIARGDKAAYAKQALENY 135 IY A+ G DF PREY GVYL+RRRESGFQLYNTLGIA+GDK AYAKQALEN+ Sbjct: 70 DVIYGVAASGAPSSGDFEFPREYRGVYLDRRRESGFQLYNTLGIAKGDKTAYAKQALENF 129 Query: 136 NFFGAPHIAVIHTNEPLGIYGAIDCGGYVSNFMLAAQALGLGTIPQAAIARHSGLIRRHF 195 NFFGAPH+A+I T+E LG+YGAIDCGGYV++FMLAAQALGL T+PQAA+A HSG++R HF Sbjct: 130 NFFGAPHVAIITTDEALGVYGAIDCGGYVTSFMLAAQALGLATVPQAALAFHSGVVREHF 189 Query: 196 NLADDRRVVCGISFGYADHAQKVNSYRTSRATVEDTVTFVD 236 L D RRVVCGISFG+AD K NSYRT+RA + D TFV+ Sbjct: 190 GLGDGRRVVCGISFGFADREHKANSYRTNRAPIGDVATFVE 230 >ref|YP_782728.1| nitroreductase [Rhodopseudomonas palustris BisA53] gb|ABJ07748.1| nitroreductase [Rhodopseudomonas palustris BisA53] Length = 228 Score = 314 bits (804), Expect = 8e-84, Method: Compositional matrix adjust. Identities = 150/220 (68%), Positives = 178/220 (80%) Query: 16 IGALEELLNERYSVRAFQPREVPREVIEHILSTAQRTASWCNSQPWQVLIVSGEAKERFR 75 I LE+LL +RYS RA++P VPR +IE +L+ AQ+TASWCNSQPWQV+I SGE +FR Sbjct: 7 IDVLEDLLAQRYSCRAYKPDPVPRAIIERVLNAAQKTASWCNSQPWQVVITSGEETTKFR 66 Query: 76 KAIYAEASRGLAEDYDFTPPREYVGVYLERRRESGFQLYNTLGIARGDKAAYAKQALENY 135 A+YA A+ G DF PREY GVYLERRRESGFQLYN LGIA+GDK AYAKQALEN+ Sbjct: 67 DALYAAAASGAPAGGDFPFPREYRGVYLERRRESGFQLYNALGIAKGDKLAYAKQALENF 126 Query: 136 NFFGAPHIAVIHTNEPLGIYGAIDCGGYVSNFMLAAQALGLGTIPQAAIARHSGLIRRHF 195 NFFGAPH+A+I T+E LG+YGA+DCGGYV+NFMLAAQALGL T+PQAA+A S ++R+HF Sbjct: 127 NFFGAPHVAIISTDEALGVYGAVDCGGYVNNFMLAAQALGLATVPQAALAFQSAVVRKHF 186 Query: 196 NLADDRRVVCGISFGYADHAQKVNSYRTSRATVEDTVTFV 235 L DDRRVVCGISFGY D K+NSYRTSRA + + TFV Sbjct: 187 GLGDDRRVVCGISFGYPDREHKINSYRTSRAAIGEAATFV 226 >ref|YP_001242903.1| hypothetical protein BBta_7115 [Bradyrhizobium sp. BTAi1] gb|ABQ38997.1| hypothetical protein BBta_7115 [Bradyrhizobium sp. BTAi1] Length = 215 Score = 312 bits (799), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 145/214 (67%), Positives = 171/214 (79%) Query: 23 LNERYSVRAFQPREVPREVIEHILSTAQRTASWCNSQPWQVLIVSGEAKERFRKAIYAEA 82 + R+S R+F+ VPR IE IL+ AQ+TASWCNSQPW+V I SG A E FRK +YA A Sbjct: 1 MAARFSCRSFRIDAVPRATIERILTAAQKTASWCNSQPWRVEITSGAATEAFRKVMYAAA 60 Query: 83 SRGLAEDYDFTPPREYVGVYLERRRESGFQLYNTLGIARGDKAAYAKQALENYNFFGAPH 142 S G DF PREY GVYL+RRRESGFQLYN LGI RGDKA YA+QALENYNFFGAPH Sbjct: 61 SSGKPAAGDFPFPREYKGVYLDRRRESGFQLYNALGIERGDKAGYARQALENYNFFGAPH 120 Query: 143 IAVIHTNEPLGIYGAIDCGGYVSNFMLAAQALGLGTIPQAAIARHSGLIRRHFNLADDRR 202 +A+IH++E LG YGAIDCGGYV++F+LAAQALG+ T+PQAA+A HS L+R+HF LADDR+ Sbjct: 121 VAIIHSDEALGTYGAIDCGGYVTSFLLAAQALGVATVPQAALAFHSDLVRQHFGLADDRK 180 Query: 203 VVCGISFGYADHAQKVNSYRTSRATVEDTVTFVD 236 +VCGISFGY D + K NSYRTSRA + DT FVD Sbjct: 181 IVCGISFGYPDLSHKANSYRTSRAAIADTARFVD 214