BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0258.0007.p (361 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774442.1| acyl-CoA synthetase [Bradyrhizobium japonicum U... 502 e-140 ref|YP_001203088.1| acyl-CoA synthetase [Bradyrhizobium sp. ORS ... 457 e-126 ref|ZP_08628334.1| feruloyl-CoA synthetase [Bradyrhizobiaceae ba... 453 e-125 ref|YP_001242907.1| acyl-CoA synthetase [Bradyrhizobium sp. BTAi... 453 e-125 ref|YP_782731.1| acyl-CoA synthetase [Rhodopseudomonas palustris... 444 e-122 >ref|NP_774442.1| acyl-CoA synthetase [Bradyrhizobium japonicum USDA 110] dbj|BAC53067.1| long-chain-fatty-acid-CoA ligase [Bradyrhizobium japonicum USDA 110] Length = 623 Score = 502 bits (1292), Expect = e-140, Method: Compositional matrix adjust. Identities = 260/315 (82%), Positives = 281/315 (89%), Gaps = 5/315 (1%) Query: 5 AAMLTKPAFRKVDWLARDIAVERRGDGTVVLKSRIPLQAYEMHIPAYLAKWAARAPERIW 64 AA++TKPAFRKV+WLARDI VERRGDGTVVLKSRIPLQ YE HIPA LAKWA +APERIW Sbjct: 6 AAVMTKPAFRKVEWLARDIDVERRGDGTVVLKSRIPLQPYEKHIPASLAKWATQAPERIW 65 Query: 65 LAQRGGPDRQWRKVSYGEAKRTVDALTQGLLDLGVGDGRPVAILSGNSIEHALMTQAAMQ 124 LAQRGGP+R+WRKVSYGEAKRTVDALTQGLLDLG+ DGRPV I+SGNSIEHALMTQAAMQ Sbjct: 66 LAQRGGPNREWRKVSYGEAKRTVDALTQGLLDLGL-DGRPVTIVSGNSIEHALMTQAAMQ 124 Query: 125 ARLPAAPVSPAYSLMSQDHLKLKYLFNLIKPAVVMVQDGPTFEKALKAIDLTGITVVHVL 184 AR+PAAPVSPAYSLMS DH+KLKYLF+LIKPAVVMVQDGPTFEKALKAIDLTG+TVVHVL Sbjct: 125 ARVPAAPVSPAYSLMSHDHVKLKYLFDLIKPAVVMVQDGPTFEKALKAIDLTGVTVVHVL 184 Query: 185 RPCEGIKSMAFADLAATPVTAAVGESIAKITPRTVGKLLFTSGSTGMPKAVINTQEMMCA 244 RPCEGIKS++FA+LAATPVT AV ESIA+ITP TVGKLLFTSGSTGMPKAVINTQ MMCA Sbjct: 185 RPCEGIKSVSFAELAATPVTGAVEESIARITPDTVGKLLFTSGSTGMPKAVINTQAMMCA 244 Query: 245 NAAMMMQVRPRETEGPIPTVLDWMPWNHTMGGNAAFNPVLVDGGTLYITTARPCRASSRR 304 NAAMMMQVRPR+ GPI T+LDWMPWNHTMGGNAAF+P+LVDGGTLYI RP Sbjct: 245 NAAMMMQVRPRDPGGPISTMLDWMPWNHTMGGNAAFHPILVDGGTLYIDDGRPMPGQFEE 304 Query: 305 PSGTCARCRRPITPT 319 R R I+PT Sbjct: 305 ----TLRNLREISPT 315 >ref|YP_001203088.1| acyl-CoA synthetase [Bradyrhizobium sp. ORS 278] emb|CAL74851.1| Putative long-chain-fatty-acid--CoA ligase (Long-chain acyl-CoA synthetase) [Bradyrhizobium sp. ORS 278] Length = 622 Score = 457 bits (1177), Expect = e-126, Method: Compositional matrix adjust. Identities = 236/298 (79%), Positives = 257/298 (86%), Gaps = 2/298 (0%) Query: 1 MDGGAA-MLTKPAFRKVDWLARDIAVERRGDGTVVLKSRIPLQAYEMHIPAYLAKWAARA 59 MDG AA +L KPAFRKV+WLARDI VERR DG +V+KSRIPLQAYE H+ A LA+WA A Sbjct: 1 MDGTAAKVLEKPAFRKVNWLARDIDVERRADGVIVIKSRIPLQAYEPHLAAPLARWAKEA 60 Query: 60 PERIWLAQRGGPDRQWRKVSYGEAKRTVDALTQGLLDLGVGDGRPVAILSGNSIEHALMT 119 P R WLAQR GP+RQWRK+ Y EAKRT+D LTQGLL+L + DGRPVAILSGNSIEHALMT Sbjct: 61 PTRTWLAQRSGPERQWRKLPYAEAKRTIDGLTQGLLNLKL-DGRPVAILSGNSIEHALMT 119 Query: 120 QAAMQARLPAAPVSPAYSLMSQDHLKLKYLFNLIKPAVVMVQDGPTFEKALKAIDLTGIT 179 AAMQAR PAAPVSPAYSLMS DHLKLKYLF L+KP VVMVQDGPTFEKALKA+DL G+T Sbjct: 120 FAAMQARSPAAPVSPAYSLMSHDHLKLKYLFGLVKPGVVMVQDGPTFEKALKALDLDGVT 179 Query: 180 VVHVLRPCEGIKSMAFADLAATPVTAAVGESIAKITPRTVGKLLFTSGSTGMPKAVINTQ 239 VVHV RPCEGI S++FADLAATPVT V SIA+ITP TVGKLLFTSGSTGMPKAVINTQ Sbjct: 180 VVHVARPCEGIPSVSFADLAATPVTGDVDASIAQITPETVGKLLFTSGSTGMPKAVINTQ 239 Query: 240 EMMCANAAMMMQVRPRETEGPIPTVLDWMPWNHTMGGNAAFNPVLVDGGTLYITTARP 297 MMCANAAMMMQ RPR + P+ TVLDWMPWNHTMGGNAAFNP+L+DGGTLYI RP Sbjct: 240 RMMCANAAMMMQTRPRAPDAPVSTVLDWMPWNHTMGGNAAFNPLLIDGGTLYIDDGRP 297 >ref|ZP_08628334.1| feruloyl-CoA synthetase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP09243.1| feruloyl-CoA synthetase [Bradyrhizobiaceae bacterium SG-6C] Length = 624 Score = 453 bits (1166), Expect = e-125, Method: Compositional matrix adjust. Identities = 224/294 (76%), Positives = 244/294 (82%) Query: 5 AAMLTKPAFRKVDWLARDIAVERRGDGTVVLKSRIPLQAYEMHIPAYLAKWAARAPERIW 64 +A L KP FRK++WL RDIAVERR DG +V+KSR PL AY HIPA LAKWA P+ W Sbjct: 6 SAGLAKPPFRKIEWLPRDIAVERRDDGVIVIKSRTPLDAYGPHIPASLAKWALERPDHTW 65 Query: 65 LAQRGGPDRQWRKVSYGEAKRTVDALTQGLLDLGVGDGRPVAILSGNSIEHALMTQAAMQ 124 LAQR GP+RQWRKVSYGEAKRTVDALTQ LL++ V + RP+AILSGNS+EHALMTQAAMQ Sbjct: 66 LAQRRGPERQWRKVSYGEAKRTVDALTQALLNMNVPEDRPIAILSGNSLEHALMTQAAMQ 125 Query: 125 ARLPAAPVSPAYSLMSQDHLKLKYLFNLIKPAVVMVQDGPTFEKALKAIDLTGITVVHVL 184 AR+PAAPVSPAYSLMSQDH KLKYLFNL+KPAVVMVQDGP FEKAL A+DL GITV+HV Sbjct: 126 ARIPAAPVSPAYSLMSQDHAKLKYLFNLVKPAVVMVQDGPAFEKALAALDLNGITVIHVD 185 Query: 185 RPCEGIKSMAFADLAATPVTAAVGESIAKITPRTVGKLLFTSGSTGMPKAVINTQEMMCA 244 RP IKS+A+ADLAAT V AV SIAKITP TVGKLLFTSGSTGMPKAVINTQ+MMCA Sbjct: 186 RPPVNIKSVAYADLAATKVAPAVEASIAKITPDTVGKLLFTSGSTGMPKAVINTQKMMCA 245 Query: 245 NAAMMMQVRPRETEGPIPTVLDWMPWNHTMGGNAAFNPVLVDGGTLYITTARPC 298 NA MMMQVRPR LDWMPWNHTMGGNA FNPVLV+GGTLYI RP Sbjct: 246 NARMMMQVRPRPPGHSEAIYLDWMPWNHTMGGNALFNPVLVEGGTLYIDDGRPL 299 >ref|YP_001242907.1| acyl-CoA synthetase [Bradyrhizobium sp. BTAi1] gb|ABQ39001.1| Putative long-chain-fatty-acid--CoA ligase (Long-chain acyl-CoA synthetase) [Bradyrhizobium sp. BTAi1] Length = 622 Score = 453 bits (1165), Expect = e-125, Method: Compositional matrix adjust. Identities = 231/298 (77%), Positives = 257/298 (86%), Gaps = 2/298 (0%) Query: 1 MDGGAA-MLTKPAFRKVDWLARDIAVERRGDGTVVLKSRIPLQAYEMHIPAYLAKWAARA 59 MDG AA +L KPAFRKV+WLARDI VERR DG +++KSRIPLQAYE H+ A LA+WA A Sbjct: 1 MDGTAAKVLEKPAFRKVNWLARDIDVERRADGVIIIKSRIPLQAYEPHLAAPLARWAKEA 60 Query: 60 PERIWLAQRGGPDRQWRKVSYGEAKRTVDALTQGLLDLGVGDGRPVAILSGNSIEHALMT 119 P R WLAQR GP+RQWRK+SY +AKRTVD LTQGLL+L + DGRPVAILSGNSIEHAL+T Sbjct: 61 PTRTWLAQRSGPERQWRKLSYADAKRTVDGLTQGLLNLKL-DGRPVAILSGNSIEHALLT 119 Query: 120 QAAMQARLPAAPVSPAYSLMSQDHLKLKYLFNLIKPAVVMVQDGPTFEKALKAIDLTGIT 179 AAMQARLPAAPVSPAYSLMS DHLKLKYLF L+KP VVMVQDGPTFEKALKA+DL G+T Sbjct: 120 MAAMQARLPAAPVSPAYSLMSHDHLKLKYLFGLVKPGVVMVQDGPTFEKALKALDLDGVT 179 Query: 180 VVHVLRPCEGIKSMAFADLAATPVTAAVGESIAKITPRTVGKLLFTSGSTGMPKAVINTQ 239 V+HV RPC+G+ S+ FADLAATPVT V S+A+ITP TVGKLLFTSGSTGMPKAVINTQ Sbjct: 180 VIHVARPCDGVPSVGFADLAATPVTLEVAASVAQITPETVGKLLFTSGSTGMPKAVINTQ 239 Query: 240 EMMCANAAMMMQVRPRETEGPIPTVLDWMPWNHTMGGNAAFNPVLVDGGTLYITTARP 297 MMCANAAMMMQ RPR + PI TVLDWMPWNHTMGGNA FNP+L++GGTLYI RP Sbjct: 240 RMMCANAAMMMQTRPRGPDAPISTVLDWMPWNHTMGGNALFNPLLIEGGTLYIDDGRP 297 >ref|YP_782731.1| acyl-CoA synthetase [Rhodopseudomonas palustris BisA53] gb|ABJ07751.1| AMP-dependent synthetase and ligase [Rhodopseudomonas palustris BisA53] Length = 624 Score = 444 bits (1141), Expect = e-122, Method: Compositional matrix adjust. Identities = 214/293 (73%), Positives = 241/293 (82%) Query: 5 AAMLTKPAFRKVDWLARDIAVERRGDGTVVLKSRIPLQAYEMHIPAYLAKWAARAPERIW 64 AA+L KPAFRK++WL R+I V+RR DGTVV++SR PL AY HIP LAKWA AP+RIW Sbjct: 6 AAVLEKPAFRKINWLPREIDVDRRADGTVVIQSRFPLDAYAPHIPFSLAKWAREAPDRIW 65 Query: 65 LAQRGGPDRQWRKVSYGEAKRTVDALTQGLLDLGVGDGRPVAILSGNSIEHALMTQAAMQ 124 LAQR G RQW K+SY EAKR+VDALTQ +LDL + +GRPVAILSGNSIEHALMTQAAMQ Sbjct: 66 LAQRRGEARQWHKLSYAEAKRSVDALTQAILDLKLPEGRPVAILSGNSIEHALMTQAAMQ 125 Query: 125 ARLPAAPVSPAYSLMSQDHLKLKYLFNLIKPAVVMVQDGPTFEKALKAIDLTGITVVHVL 184 AR+PAAPVSPAYSLMS DH KLKYLF+L+KPA+V+VQDG F +AL A+DLTG+ VVHV Sbjct: 126 ARIPAAPVSPAYSLMSHDHQKLKYLFDLVKPALVLVQDGVAFSRALHALDLTGVKVVHVA 185 Query: 185 RPCEGIKSMAFADLAATPVTAAVGESIAKITPRTVGKLLFTSGSTGMPKAVINTQEMMCA 244 P +GI S+AFADLAAT VT V SIA+ITP TVGKLLFTSGSTGMPKAVINTQ MMCA Sbjct: 186 NPADGIDSIAFADLAATKVTDEVAASIAQITPDTVGKLLFTSGSTGMPKAVINTQRMMCA 245 Query: 245 NAAMMMQVRPRETEGPIPTVLDWMPWNHTMGGNAAFNPVLVDGGTLYITTARP 297 N AMM+QVRPR+ P LDWMPWNHTMGGNA FNP L +GGTLYI RP Sbjct: 246 NVAMMLQVRPRDPNAPPSIYLDWMPWNHTMGGNALFNPALTEGGTLYIDDGRP 298