BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0013.p (284 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774589.1| phosphonate metabolism protein [Bradyrhizobium ... 381 e-104 ref|YP_001207264.1| putative metal-dependent hydrolase involved ... 355 5e-96 ref|YP_004107122.1| phosphonate metabolism protein PhnM [Rhodops... 351 9e-95 ref|YP_534276.1| phosphonate metabolism PhnM [Rhodopseudomonas p... 349 3e-94 ref|NP_946055.1| phosphonate ABC transporter permease [Rhodopseu... 344 1e-92 >ref|NP_774589.1| phosphonate metabolism protein [Bradyrhizobium japonicum USDA 110] dbj|BAC53214.1| phosphonate metabolism protein [Bradyrhizobium japonicum USDA 110] Length = 397 Score = 381 bits (979), Expect = e-104, Method: Compositional matrix adjust. Identities = 199/284 (70%), Positives = 236/284 (83%), Gaps = 7/284 (2%) Query: 8 RGECAETARRXPQLAADTRFHLRHETHNLEAEAEIIQWLAEGRIDLFAFNDHSN------ 61 RG R+ PQ AADTRFHLRHET+NL+AEAEI +WLAEGR+DLFAFNDH + Sbjct: 114 RGLMEAIERQRPQFAADTRFHLRHETYNLDAEAEISRWLAEGRVDLFAFNDHMDGTVADM 173 Query: 62 VKPKKRNQLAERTGLSVEAVNDMLDRVVARRPEVPASIARLAAAARAADIRMLSHDDNSP 121 KP+KRN++ ERTGLS E + ++DRV++R +VPAS++RLAA+ARAAD+RMLSHDD +P Sbjct: 174 AKPRKRNRMVERTGLSSEDFDGLVDRVISRAADVPASVSRLAASARAADVRMLSHDDATP 233 Query: 122 AMRQEFRALGAVIAEFPVNEETARDAADAGDFIVFGAPNVVRGGSHTGWTKASDMIAQGL 181 AMR+EFR LGA IAEFP+NEETAR AA GD IV+GAPNVVRGGSHTGWT+ASDMIA+GL Sbjct: 234 AMRREFRELGADIAEFPINEETARAAASHGDAIVYGAPNVVRGGSHTGWTRASDMIAKGL 293 Query: 182 CSVLASDYYYPAQLLAAFRLVADGILPLARAWDLISSAPARAAGLAYRGELAAGRRADII 241 CSVLASDYYYPAQLLAAFRL ADG+LPL AW L+S+ PARAAGLA RG LA GRRADI+ Sbjct: 294 CSVLASDYYYPAQLLAAFRLAADGVLPLTEAWSLVSAEPARAAGLADRGILAEGRRADIL 353 Query: 242 VVDDKVPLRPRIVAVIAAGRLVHLADASCLVH-SLAPRKTVAAA 284 +VDD VPLRPR+VAVI+ G+LVHL DA+ L+ + APR+ V AA Sbjct: 354 LVDDTVPLRPRLVAVISGGKLVHLTDATRLLSAAAAPREAVVAA 397 >ref|YP_001207264.1| putative metal-dependent hydrolase involved in phosphonate metabolism/PhnM protein [Bradyrhizobium sp. ORS 278] emb|CAL79047.1| putative Metal-dependent hydrolase involved in phosphonate metabolism; PhnM protein [Bradyrhizobium sp. ORS 278] Length = 395 Score = 355 bits (911), Expect = 5e-96, Method: Compositional matrix adjust. Identities = 184/269 (68%), Positives = 211/269 (78%), Gaps = 8/269 (2%) Query: 10 ECAETARRXPQLAADTRFHLRHETHNLEAEAEIIQWLAEGRIDLFAFNDHSNV------K 63 E ET R P+LAADTRFHLRHET+NL+ E EI Q LA RIDLFAFNDH + K Sbjct: 118 EAIETLR--PRLAADTRFHLRHETYNLDDEDEIRQLLAARRIDLFAFNDHMDSTVASLEK 175 Query: 64 PKKRNQLAERTGLSVEAVNDMLDRVVARRPEVPASIARLAAAARAADIRMLSHDDNSPAM 123 P KR ++ +RTGLS + + ++ R+ AR VP+SI+RLA AR A +RMLSHDD SP M Sbjct: 176 PHKRQRMVDRTGLSGDEFDRLVGRIAARADAVPSSISRLAETARTAGVRMLSHDDESPEM 235 Query: 124 RQEFRALGAVIAEFPVNEETARDAADAGDFIVFGAPNVVRGGSHTGWTKASDMIAQGLCS 183 R+ FR G IAEFPVNEETAR+AA GD IVFGAPNVVRGGSHTGWTKASDMIA+GLCS Sbjct: 236 RRAFRGQGVSIAEFPVNEETAREAAAGGDAIVFGAPNVVRGGSHTGWTKASDMIAKGLCS 295 Query: 184 VLASDYYYPAQLLAAFRLVADGILPLARAWDLISSAPARAAGLAYRGELAAGRRADIIVV 243 VLASDYYYPA LLAA+RLVADG+LPLARAW LIS+ PA+AAGL+ RG LA G+RADI++V Sbjct: 296 VLASDYYYPAPLLAAYRLVADGVLPLARAWSLISAGPAQAAGLSDRGTLAPGQRADIVLV 355 Query: 244 DDKVPLRPRIVAVIAAGRLVHLADASCLV 272 DD +RPRIVAVIAAGRLVHL DA L Sbjct: 356 DDSSSMRPRIVAVIAAGRLVHLTDADRLT 384 >ref|YP_004107122.1| phosphonate metabolism protein PhnM [Rhodopseudomonas palustris DX-1] gb|ADU42389.1| phosphonate metabolism protein PhnM [Rhodopseudomonas palustris DX-1] Length = 397 Score = 351 bits (900), Expect = 9e-95, Method: Compositional matrix adjust. Identities = 181/276 (65%), Positives = 212/276 (76%), Gaps = 14/276 (5%) Query: 11 CAETARR--------XPQLAADTRFHLRHETHNLEAEAEIIQWLAEGRIDLFAFNDHSN- 61 AE ARR P+L+ADTR HLRHET NL+A EI QW+ + RIDLFAFNDH + Sbjct: 109 SAENARRMLDAIDLTKPRLSADTRIHLRHETFNLDAVDEIAQWIGDRRIDLFAFNDHMDS 168 Query: 62 -----VKPKKRNQLAERTGLSVEAVNDMLDRVVARRPEVPASIARLAAAARAADIRMLSH 116 KP+KRN++ ERTGLS EA + +++R+ AR EVP + RLAA AR + MLSH Sbjct: 169 TLANLAKPQKRNRMVERTGLSDEAFDRLVERIAARGDEVPQANRRLAAIAREVSMPMLSH 228 Query: 117 DDNSPAMRQEFRALGAVIAEFPVNEETARDAADAGDFIVFGAPNVVRGGSHTGWTKASDM 176 DD++PAMRQ +R LG IAEFP EETARDAA GDFIVFG+PNVVRGGSHTGWTKA+DM Sbjct: 229 DDDTPAMRQGYRDLGVGIAEFPTTEETARDAALHGDFIVFGSPNVVRGGSHTGWTKAADM 288 Query: 177 IAQGLCSVLASDYYYPAQLLAAFRLVADGILPLARAWDLISSAPARAAGLAYRGELAAGR 236 IA+GLCSVLASDYYYPA LLAAFRLV DGILPL +AW LIS PARAAGLA RG +A+G+ Sbjct: 289 IAKGLCSVLASDYYYPAPLLAAFRLVHDGILPLEQAWKLISENPARAAGLAERGTIASGQ 348 Query: 237 RADIIVVDDKVPLRPRIVAVIAAGRLVHLADASCLV 272 RAD+++VDD+V LRPRIVAVIA GRLVH+ D V Sbjct: 349 RADVLLVDDRVALRPRIVAVIAGGRLVHVDDPERFV 384 >ref|YP_534276.1| phosphonate metabolism PhnM [Rhodopseudomonas palustris BisB18] gb|ABD89957.1| Phosphonate metabolism PhnM [Rhodopseudomonas palustris BisB18] Length = 396 Score = 349 bits (895), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 178/265 (67%), Positives = 206/265 (77%), Gaps = 8/265 (3%) Query: 13 ETARRXPQLAADTRFHLRHETHNLEAEAEIIQWLAEGRIDLFAFNDHSNV------KPKK 66 ET R PQLAADTRFHLRHET+NL+AE I W+A GRIDL AFNDH+ KP K Sbjct: 120 ETLR--PQLAADTRFHLRHETYNLDAEHTIKHWIAAGRIDLLAFNDHTESTVAALDKPAK 177 Query: 67 RNQLAERTGLSVEAVNDMLDRVVARRPEVPASIARLAAAARAADIRMLSHDDNSPAMRQE 126 RN++ ER+G+S + + ++ VVAR EVP SIARLA ARA ++MLSHDD++P +RQ Sbjct: 178 RNRMVERSGVSHDEFDRIVSHVVARHHEVPGSIARLAETARAVQLKMLSHDDDTPELRQA 237 Query: 127 FRALGAVIAEFPVNEETARDAADAGDFIVFGAPNVVRGGSHTGWTKASDMIAQGLCSVLA 186 FR G IAEFPVNE TAR A D GD VFGAPNVVRGGSHTGWTKA+DMIA GLCS+LA Sbjct: 238 FRDQGVAIAEFPVNEPTARAAIDGGDVTVFGAPNVVRGGSHTGWTKAADMIANGLCSILA 297 Query: 187 SDYYYPAQLLAAFRLVADGILPLARAWDLISSAPARAAGLAYRGELAAGRRADIIVVDDK 246 SDYYYPA LLAAFRLVADG+LPLA AW L+SSAPA+A GL RG +A GRRAD+I+VDD Sbjct: 298 SDYYYPAPLLAAFRLVADGVLPLAEAWTLVSSAPAKAVGLTDRGVIAEGRRADLILVDDA 357 Query: 247 VPLRPRIVAVIAAGRLVHLADASCL 271 V LRPR+VA IAAGRLV+L +A L Sbjct: 358 VALRPRLVAAIAAGRLVYLREAERL 382 >ref|NP_946055.1| phosphonate ABC transporter permease [Rhodopseudomonas palustris CGA009] emb|CAE26146.1| possible phosphonate ABC transporter, permease component [Rhodopseudomonas palustris CGA009] Length = 397 Score = 344 bits (882), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 171/260 (65%), Positives = 206/260 (79%), Gaps = 6/260 (2%) Query: 19 PQLAADTRFHLRHETHNLEAEAEIIQWLAEGRIDLFAFNDHSNV------KPKKRNQLAE 72 P+L+ADTR HLRHET NL+A EI QW+ + R+DLFAFNDH + KP+KRN++ E Sbjct: 125 PRLSADTRIHLRHETFNLDAVDEIAQWIGDRRVDLFAFNDHMDTTLANLAKPQKRNRMVE 184 Query: 73 RTGLSVEAVNDMLDRVVARRPEVPASIARLAAAARAADIRMLSHDDNSPAMRQEFRALGA 132 RTGLS + + +++R+ AR EVP + RLAA AR + MLSHDD++PA+RQ +R LG Sbjct: 185 RTGLSDDEFDRLVERIAARGDEVPQASRRLAAIAREVSMPMLSHDDDTPAIRQGYRDLGV 244 Query: 133 VIAEFPVNEETARDAADAGDFIVFGAPNVVRGGSHTGWTKASDMIAQGLCSVLASDYYYP 192 IAEFP EETARDAA GDFIVFG+PNVVRGGSHTGWTKA+DMIA+GLCSVLASDYYYP Sbjct: 245 GIAEFPTTEETARDAALHGDFIVFGSPNVVRGGSHTGWTKAADMIAKGLCSVLASDYYYP 304 Query: 193 AQLLAAFRLVADGILPLARAWDLISSAPARAAGLAYRGELAAGRRADIIVVDDKVPLRPR 252 A LLAAFRLV DGILPL +AW LIS PARAAGL+ RG +A+G+RAD+++VDD+V LRPR Sbjct: 305 APLLAAFRLVHDGILPLEQAWKLISENPARAAGLSERGTIASGQRADVLLVDDRVALRPR 364 Query: 253 IVAVIAAGRLVHLADASCLV 272 IVAVIA GRLVH+ D V Sbjct: 365 IVAVIAGGRLVHVDDPERFV 384