BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0005.p (472 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_823022.1| hypothetical protein Acid_1747 [Candidatus Soli... 468 e-129 ref|YP_004753982.1| peptidase M20 [Collimonas fungivorans Ter331... 457 e-126 ref|ZP_07674168.1| peptidase, M20/M25/M40 family [Ralstonia sp. ... 455 e-126 ref|YP_822086.1| hypothetical protein Acid_0802 [Candidatus Soli... 451 e-124 ref|YP_122591.1| hypothetical protein lpp0248 [Legionella pneumo... 450 e-124 >ref|YP_823022.1| hypothetical protein Acid_1747 [Candidatus Solibacter usitatus Ellin6076] gb|ABJ82737.1| peptidase M20 [Candidatus Solibacter usitatus Ellin6076] Length = 462 Score = 468 bits (1203), Expect = e-129, Method: Compositional matrix adjust. Identities = 238/438 (54%), Positives = 301/438 (68%), Gaps = 9/438 (2%) Query: 36 QQFARGIYQELVEINTTTATGDTQKAAEAMGARLRAAGFPEADVHVFSPAPHKGNLVARL 95 +Q AR IY++++E + TG T AEAM ARL++AGFP+AD+ V P K NLV R Sbjct: 29 RQLAREIYRQMIESKSGFTTGATTPIAEAMAARLKSAGFPDADIFVGGAIPRKANLVVRY 88 Query: 96 HGSGARKPILLVAHIDVVPALREDWTVDPFKLTEQDGYFYGRGSGDDKYMASAFITNLIR 155 HG+G RKPILL+AHIDVV ALREDWT DPF L E+DGYFYGRG+ DDK A+ +I NLIR Sbjct: 89 HGAGRRKPILLLAHIDVVEALREDWTTDPFVLIEKDGYFYGRGTMDDKAQAAVWIANLIR 148 Query: 156 YRKEGYKPDRDIIVALETDEEILDANGL--GMQWLIKNHRDLIDAEFALNEGGGVGLKSG 213 Y++E ++PDRD+IVAL DEE G G+ WL+KNHRDLI+AEFA+NEG + G Sbjct: 149 YKRENFRPDRDLIVALTADEE---GGGPYNGVDWLLKNHRDLIEAEFAVNEGARGEMIDG 205 Query: 214 KAIRNSVQTSEKVSIGYQLTVKDRGGHSSLPRKDNAIYHLAGGLVRLSNYSFPMNLNETT 273 K I N + SEKV ++L V+++GGHS+ P DNAIYHLAGGL RLS ++FP +LNE T Sbjct: 206 KRISNDMSLSEKVYSDFRLEVRNKGGHSARPVPDNAIYHLAGGLYRLSTFAFPYHLNEIT 265 Query: 274 RIYFSKTAEVDTSH-ADDIKAILAPQPDPAALARLSENPG-YNAQLRTTCVATMLEGGHA 331 R YF + A+++ A D+ I Q D AA+ R+S NA LRTTCVAT LEGGHA Sbjct: 266 RAYFRQMAKIEKGPLAADLTRI--SQGDEAAMRRVSAASAPLNAMLRTTCVATQLEGGHA 323 Query: 332 INALPQLATAKVNCRILPGEPVEGVQATIERVLADKEIAVAPTGKAVLSPPSPLNEEIMG 391 NALPQLA A VNCRILP + +E V AT+++V+AD ++ + SP SP+ +IM Sbjct: 324 TNALPQLAAANVNCRILPEDSIENVLATLKKVVADDQVVITIKTNEGASPASPMRPDIMQ 383 Query: 392 SIERLSKEFWPDASIVPVMSTGATDGSYLRNAGIPTYGHSGLAADVDDNRIHGKDERVLV 451 + ER+S WP VP M+ G +DG YLR AGIPTYG G D DD R HG+DER+ V Sbjct: 384 AFERISDIMWPGVITVPSMAVGGSDGRYLRVAGIPTYGIQGFFVDRDDVRAHGRDERMPV 443 Query: 452 KSFYEGEEYLYRLVKMLA 469 SFYEG+ +LY VK L+ Sbjct: 444 NSFYEGQTFLYEFVKSLS 461 >ref|YP_004753982.1| peptidase M20 [Collimonas fungivorans Ter331] gb|AEK63159.1| peptidase M20 [Collimonas fungivorans Ter331] Length = 469 Score = 457 bits (1176), Expect = e-126, Method: Compositional matrix adjust. Identities = 231/441 (52%), Positives = 300/441 (68%), Gaps = 1/441 (0%) Query: 30 QGLTEQQQFARGIYQELVEINTTTATGDTQKAAEAMGARLRAAGFPEADVHVFSPAPHKG 89 Q LT + Q IY+ELVEINT+ + GDT KAA AM L AGF AD+ V P P KG Sbjct: 28 QTLTPETQRYHDIYKELVEINTSHSAGDTTKAAHAMEKHLLDAGFTAADIQVIEPFPRKG 87 Query: 90 NLVARLHGSGARKPILLVAHIDVVPALREDWTVDPFKLTEQDGYFYGRGSGDDKYMASAF 149 NLV R G+G+++P+LL+AHIDVV A REDW DPF+L E GYF RGS DDK MASAF Sbjct: 88 NLVLRFKGNGSKQPLLLLAHIDVVEAKREDWKTDPFQLQETGGYFTARGSIDDKAMASAF 147 Query: 150 ITNLIRYRKEGYKPDRDIIVALETDEEILDANGLGMQWLIKNHRDLIDAEFALNEGGGVG 209 ++ L + +KEG+KP RDII+AL TDEE D G +WLI N +L+ AEF +NEGGG Sbjct: 148 VSVLSQLKKEGFKPSRDIILALTTDEERGDVESNGAKWLIANKPELLKAEFGINEGGGGE 207 Query: 210 LKSGKAIRNSVQTSEKVSIGYQLTVKDRGGHSSLPRKDNAIYHLAGGLVRLSNYSFPMNL 269 L+ GK + +Q +EK+ Y+L +D GGHSS P +N IY +A L RLS Y FP++L Sbjct: 208 LRDGKPNLHRIQVAEKIYATYELEARDAGGHSSTPTANNPIYAIAAALNRLSAYRFPVHL 267 Query: 270 NETTRIYFSKTAEVDTSH-ADDIKAILAPQPDPAALARLSENPGYNAQLRTTCVATMLEG 328 E T YF+++A A D++A+ + +PD AA+ RLS P YNAQLR+TCVATM+ Sbjct: 268 AEVTTGYFARSAPFAGGQLAADMRAVGSGKPDTAAIERLSAVPDYNAQLRSTCVATMVNA 327 Query: 329 GHAINALPQLATAKVNCRILPGEPVEGVQATIERVLADKEIAVAPTGKAVLSPPSPLNEE 388 GHA NALPQ A A VNCRILP + + + +++V+ + +IAV T K + SP SPLN + Sbjct: 328 GHAENALPQSAKATVNCRILPQDDPDDIDRQLKQVINNDKIAVRYTNKPLRSPASPLNGD 387 Query: 389 IMGSIERLSKEFWPDASIVPVMSTGATDGSYLRNAGIPTYGHSGLAADVDDNRIHGKDER 448 ++ ++E L+++ WP +VP MSTGATD SY+RNAGIP YG SGL + D R HG DER Sbjct: 388 LVKTVEALTQDMWPGVPVVPAMSTGATDSSYMRNAGIPMYGVSGLFVEPSDRRTHGLDER 447 Query: 449 VLVKSFYEGEEYLYRLVKMLA 469 V ++ Y+G E+LYRLVK LA Sbjct: 448 VEIQRLYDGREFLYRLVKRLA 468 >ref|ZP_07674168.1| peptidase, M20/M25/M40 family [Ralstonia sp. 5_7_47FAA] gb|EFP67599.1| peptidase, M20/M25/M40 family [Ralstonia sp. 5_7_47FAA] Length = 478 Score = 455 bits (1170), Expect = e-126, Method: Compositional matrix adjust. Identities = 231/456 (50%), Positives = 309/456 (67%), Gaps = 2/456 (0%) Query: 15 AAALGPALLATGAAAQGLTEQQQFARGIYQELVEINTTTATGDTQKAAEAMGARLRAAGF 74 A A ++ A+ A LT +QQ IY+ELVEINTT + GDT KAA AM RLR AGF Sbjct: 23 AGAFAQSIPASKPAPTTLTPEQQRYHDIYKELVEINTTHSAGDTTKAARAMEKRLRDAGF 82 Query: 75 PEADVHVFSPAPHKGNLVARLHGSGARKPILLVAHIDVVPALREDWTVDPFKLTEQDGYF 134 AD+ V P P KGNLV R GSGA++P+LL+AHIDVV A REDW DPF L E DGYF Sbjct: 83 AAADMQVLEPFPKKGNLVLRFKGSGAKRPMLLLAHIDVVEAKREDWKTDPFTLQETDGYF 142 Query: 135 YGRGSGDDKYMASAFITNLIRYRKEGYKPDRDIIVALETDEEILDANGLGMQWLIKNHRD 194 RG+ DDK MASAF++ L + ++EG+KP RDII+AL TDEE D G ++ N + Sbjct: 143 TARGAIDDKAMASAFVSVLGQLKQEGFKPSRDIILALTTDEERGDVPTNGAYRIVNNKPE 202 Query: 195 LIDAEFALNEGGGVGLKSGKAIRNSVQTSEKVSIGYQLTVKDRGGHSSLPRKDNAIYHLA 254 L+ AEF +NEGGG L++GK + + +Q +EK+ Y+L V+D GGHSS+P K N IY L+ Sbjct: 203 LVKAEFGINEGGGGELRNGKPVLHRIQVAEKMYTTYELEVRDVGGHSSVPTKTNPIYALS 262 Query: 255 GGLVRLSNYSFPMNLNETTRIYFSKTAEVDTSH-ADDIKAILAPQPDPAALARLSENPGY 313 L RL Y FP+ L + T+ YFS++A + T A+D++++ +PD AA+ RLS P Y Sbjct: 263 AALDRLGAYQFPVKLADVTQTYFSRSAPLATGQLAEDMRSVGTGKPDQAAIDRLSAVPFY 322 Query: 314 NAQLRTTCVATMLEGGHAINALPQLATAKVNCRILPGEPVEGVQATIERVLADKEIAVAP 373 NAQLRTTCVATM+ GHA NALPQ A A VNCRILP + + +++V+ +++I+V Sbjct: 323 NAQLRTTCVATMVNAGHAENALPQSAKATVNCRILPHDDPADIDRQLKQVIGNEKISVRY 382 Query: 374 TGKAVLSPPSPLNEEIMGSIERLSKEFWPDASIVPVMSTGATDGSYLRNAGIPTYGHSGL 433 K + SP SPLN +++ ++E L+++ W + ++P MSTGATD ++RNAGIP YG SGL Sbjct: 383 VNKPLASPASPLNGDLVKTVEALTQQMW-NVPVIPAMSTGATDSRFMRNAGIPMYGVSGL 441 Query: 434 AADVDDNRIHGKDERVLVKSFYEGEEYLYRLVKMLA 469 + D R HG DER+ + Y+G E+LYRLVK LA Sbjct: 442 FTEPADLRTHGLDERIEIARLYDGREFLYRLVKRLA 477 >ref|YP_822086.1| hypothetical protein Acid_0802 [Candidatus Solibacter usitatus Ellin6076] gb|ABJ81801.1| peptidase M20 [Candidatus Solibacter usitatus Ellin6076] Length = 456 Score = 451 bits (1161), Expect = e-124, Method: Compositional matrix adjust. Identities = 221/439 (50%), Positives = 302/439 (68%), Gaps = 4/439 (0%) Query: 33 TEQQQFARGIYQELVEINTTTATGDTQKAAEAMGARLRAAGFPEADVHVFSPAPHKGNLV 92 T Q+ AR I+++L+EINTT ++GD +AAEAM AR RAAGFP ADV V +P P KGNLV Sbjct: 17 TSDQRLAREIFKQLIEINTTDSSGDNTRAAEAMAARFRAAGFPAADVQVLAPVPRKGNLV 76 Query: 93 ARLHGSGARKPILLVAHIDVVPALREDWTVDPFKLTEQDGYFYGRGSGDDKYMASAFITN 152 RLHG+G +PIL + H+DVV A R DW DPF+ EQ+GYFYGRG+ D K + + Sbjct: 77 VRLHGTGTGRPILFLGHLDVVEARRSDWPWDPFEFREQEGYFYGRGTSDMKGDDATLVAA 136 Query: 153 LIRYRKEGYKPDRDIIVALETDEEILDANGLGMQWLIKNHRDLIDAEFALN-EGGGVGLK 211 +R ++E ++P RD+I+AL +DEE ANG+ WL+ HR LIDA+F +N + GG +K Sbjct: 137 FLRMKRENFQPSRDLILALTSDEEGGPANGV--DWLVTQHRALIDAQFCINTDAGGGHIK 194 Query: 212 SGKAIRNSVQTSEKVSIGYQLTVKDRGGHSSLPRKDNAIYHLAGGLVRLSNYSFPMNLNE 271 +GK + ++Q +EKV + ++L V + GGHSSLP KDNAIYHLA GL RLS + FP+ L + Sbjct: 195 NGKHVYMAMQAAEKVFLSFKLEVTNNGGHSSLPVKDNAIYHLADGLSRLSKFDFPVRLFD 254 Query: 272 TTRIYFSKTAEVDTSH-ADDIKAILAPQPDPAALARLSENPGYNAQLRTTCVATMLEGGH 330 TR F ++A V D+K ++ DPAA+ARLS P YNAQ+RTTCVATML GGH Sbjct: 255 VTRAAFERSANVYGGQLGADLKTMVQNPADPAAVARLSAIPFYNAQMRTTCVATMLSGGH 314 Query: 331 AINALPQLATAKVNCRILPGEPVEGVQATIERVLADKEIAVAPTGKAVLSPPSPLNEEIM 390 A NALP +ATA VNCR+LP + V+ T+++VLAD +I + + V++P P++ ++M Sbjct: 315 AENALPAVATAVVNCRLLPIDNAADVKRTLQQVLADPKIQLDEMKEPVITPYKPIDPDVM 374 Query: 391 GSIERLSKEFWPDASIVPVMSTGATDGSYLRNAGIPTYGHSGLAADVDDNRIHGKDERVL 450 ++ + WP +VP M TGA+DG YL AGIPTYG SG+ D DD R HG++ER+L Sbjct: 375 AAVAASTARLWPGLPVVPTMDTGASDGIYLIRAGIPTYGVSGIFGDEDDVRAHGRNERIL 434 Query: 451 VKSFYEGEEYLYRLVKMLA 469 V+SF +G +++Y L LA Sbjct: 435 VQSFDQGVDFIYDLATRLA 453 >ref|YP_122591.1| hypothetical protein lpp0248 [Legionella pneumophila str. Paris] emb|CAH11395.1| hypothetical protein lpp0248 [Legionella pneumophila str. Paris] Length = 469 Score = 450 bits (1158), Expect = e-124, Method: Compositional matrix adjust. Identities = 222/465 (47%), Positives = 304/465 (65%), Gaps = 3/465 (0%) Query: 6 RDTVRSLRFAAALGPALLATGAAAQGLTEQQQFARGIYQELVEINTTTATGDTQKAAEAM 65 R + + F L + A A ++QQ AR IY++ +E+ + TG T E Sbjct: 3 RKKITQISFFMILALIVGAVEAKTTRPADEQQLARSIYKQFIEVQSGFTTGSTTPLVEVA 62 Query: 66 GARLRAAGFPEADVHVFSPAPHKGNLVARLHGSGARKPILLVAHIDVVPALREDWTVDPF 125 L+ AGF D+ V +P K NLV R G+G +KP+LL+AH DVV A DW+++PF Sbjct: 63 VKYLKKAGFSNEDIFVGGASPQKANLVVRYRGTGEKKPLLLLAHTDVVEAKASDWSMNPF 122 Query: 126 KLTEQDGYFYGRGSGDDKYMASAFITNLIRYRKEGYKPDRDIIVALETDEEILDANGLGM 185 +LTE+DGYFYGRG+ DDK A+ +I NLI+Y++EG+KP RDIIVAL DEE G+ Sbjct: 123 QLTEKDGYFYGRGTLDDKAQAAIWIANLIQYKQEGFKPKRDIIVALTADEEGSSPYN-GI 181 Query: 186 QWLIKNHRDLIDAEFALNEGGGVGLKSGKAIRNSVQTSEKVSIGYQLTVKDRGGHSSLPR 245 WLIKNH+DLI+A+FALNEGG L +GK I ++Q SEK + Y L V+++GGHSSLP Sbjct: 182 SWLIKNHKDLIEADFALNEGGWGDLANGKKISQNIQVSEKYIVNYNLEVRNKGGHSSLPT 241 Query: 246 KDNAIYHLAGGLVRLSNYSFPMNLNETTRIYFSKTAEVDTSHADDIKAILAPQPDPAALA 305 KDNAIY LAG L RLS ++FP+ N+ T YF + A ++T + + A + A+ Sbjct: 242 KDNAIYRLAGALERLSKFNFPLKTNDVTAAYFKQMAAIETGPLK-TEMVEASKGSKQAMQ 300 Query: 306 RLSEN-PGYNAQLRTTCVATMLEGGHAINALPQLATAKVNCRILPGEPVEGVQATIERVL 364 RL+++ P +NA LRTTC T+LEGGHA+NALPQLA +NCR+LP + E V+ +++ + Sbjct: 301 RLADSSPQWNAILRTTCTPTLLEGGHAMNALPQLAAVTINCRVLPEDSPEMVEQSLKTAI 360 Query: 365 ADKEIAVAPTGKAVLSPPSPLNEEIMGSIERLSKEFWPDASIVPVMSTGATDGSYLRNAG 424 D EI + GK P SPL+ EI+ +I L++ +WP+ +P+M TGATDG YLR+ G Sbjct: 361 NDPEITLKRIGKLSRGPSSPLSPEILKTITHLTQTYWPEVPAIPIMVTGATDGRYLRSVG 420 Query: 425 IPTYGHSGLAADVDDNRIHGKDERVLVKSFYEGEEYLYRLVKMLA 469 IPTYG GL D DD R HG+DER+ V+SFYE + +LY LVK L+ Sbjct: 421 IPTYGVMGLFLDRDDFRAHGRDERISVESFYEAQAFLYDLVKQLS 465