BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0025.p (508 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_530750.1| AMP-dependent synthetase and ligase [Rhodopseud... 486 e-135 ref|ZP_01262572.1| putative long-chain-fatty-acid-CoA ligase [Vi... 298 1e-78 ref|ZP_06181106.1| putative long-chain-fatty-acid-CoA ligase [Vi... 297 3e-78 ref|YP_703737.1| long-chain-fatty-acid--CoA ligase [Rhodococcus ... 295 1e-77 ref|ZP_08155872.1| long-chain-fatty-acid-CoA ligase [Rhodococcus... 292 1e-76 >ref|YP_530750.1| AMP-dependent synthetase and ligase [Rhodopseudomonas palustris BisB18] gb|ABD86431.1| AMP-dependent synthetase and ligase [Rhodopseudomonas palustris BisB18] Length = 514 Score = 486 bits (1250), Expect = e-135, Method: Compositional matrix adjust. Identities = 249/506 (49%), Positives = 337/506 (66%), Gaps = 10/506 (1%) Query: 1 VKLASYLAAQAQAFPDKAALVCGETRVTFRQLYEDSDRIATALRKSGVAIGDRVAMCLPN 60 ++L + L AQA FPDK ALVCG R+ +R L +A+ LR+ G+ GDR+ + L N Sbjct: 1 MRLETILRAQAVRFPDKTALVCGNERIAYRDLERRIGHVASGLRQHGLGAGDRIVVFLNN 60 Query: 61 CCEFVTVFFAIVKSGALAMPINMRLSTNEIATIFADAAPAAVFFSEGEREVVDHICRGGA 120 E V +F+A GA+ +P+ RL+++E+ I AD+ P+A+ F EG + + + A Sbjct: 61 GVEIVELFYAAFSLGAIVVPVTTRLTSHEVQHICADSQPSAIAF-EGPGDAIQGVL---A 116 Query: 121 VR----RFVVGPSDDPTELSIASMLACQPEPIELPS-EHDDCMISYTSGTTGHPKGVITT 175 V R VG S + + AS+ + P+ L S + DD +I YTSGTTG PKG I T Sbjct: 117 VHPDALRIAVGQSAEGA-IDYASLREAEIAPLPLLSVQSDDAVIMYTSGTTGSPKGAIIT 175 Query: 176 QANYIVGNGFLNAMYWKLSPDDRIVVTTPLAHRTAFARVGNMAVLGATLYIMPRFDVNEA 235 AN + + F+NA+ W +S DDR +VTTPLAHRT FAR+ N LG TL +M +FD + Sbjct: 176 HANIVTQHCFINAVEWGISRDDRYLVTTPLAHRTGFARLSNALTLGGTLVVMKKFDPKQT 235 Query: 236 MQLIEAEKISLISIVPTVGRMMLPVIEAEPTRFSSLRVLVATGEAFPAEIKRRLLKALPK 295 + I+ E+IS+I +VPTV RMMLP IEA+P + +SLR +V TGEAFP E+KRR + LP+ Sbjct: 236 IDTIDREQISVIGMVPTVCRMMLPQIEADPDKCASLRRIVVTGEAFPVELKRRFIALLPQ 295 Query: 296 LEIFSFFAMTEAGSLAMLKPDEQFDHGASVGRVTPGVEMRLVDADGQTVNTGEAGEIWVR 355 + + SFFAMTE G + L +EQFDH AS+GR TPGVE+R+VD G V TGEAGE+ VR Sbjct: 296 VRLVSFFAMTEVGGVTSLSHEEQFDHAASIGRPTPGVEVRIVDDAGNAVATGEAGELLVR 355 Query: 356 CGLPGQYLLFRTYFNQPQAMRDAFQEGWFKTGDIGRFDADGFLYIVDRKKDMVLSGGYNI 415 G PG+Y + R Y+N+P+ A ++GW +TGD+ R D +G+LYIVDRKKDMVLSGG+NI Sbjct: 356 VGEPGRYSVMRGYYNRPEETAKAIEDGWIRTGDVARADDEGYLYIVDRKKDMVLSGGFNI 415 Query: 416 YSKEVEQVLLRYSGVADAAVVGVPDDVYGEAVAAFIEPEVDVMLTEDELIRWCASELASY 475 Y+KEVE LL VA+AAV+G+PD +YGEAV AF+EP + T ++ +A Y Sbjct: 416 YTKEVELALLANPVVAEAAVIGIPDAIYGEAVVAFVEPLPGMTPTPQSIVDGVRGLVAGY 475 Query: 476 KKPKHVVFLRELPRNSTGKVIKTELR 501 KKPK+V + ELPRNS GKV+K ELR Sbjct: 476 KKPKYVFIVDELPRNSLGKVLKRELR 501 >ref|ZP_01262572.1| putative long-chain-fatty-acid-CoA ligase [Vibrio alginolyticus 12G01] gb|EAS74095.1| putative long-chain-fatty-acid-CoA ligase [Vibrio alginolyticus 12G01] Length = 513 Score = 298 bits (764), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 183/517 (35%), Positives = 282/517 (54%), Gaps = 25/517 (4%) Query: 1 VKLASYLAAQAQAFPDKAALVCGETRVTFRQLYEDSDRIATALRKSGVAIGDRVAMCLPN 60 + LA+ L A + PDK AL+CG ++ + Q + +IA +L GV GDRVA+ PN Sbjct: 2 MNLAAALQRNAASKPDKTALICGAEKINYEQFDAVAGKIAASLINKGVKPGDRVAISCPN 61 Query: 61 CCEFVTVFFAIVKSGALAMPINMRLSTNEIATIFADAAPAAVFFSEGEREV--------- 111 F V++AI K+GA+A+P+N+ L EI D+ F EG +E+ Sbjct: 62 NPFFAFVYYAIQKAGAVAVPLNVLLKAREIKYHLEDSKAKFYFCFEGTKELPMAKEGVKA 121 Query: 112 ---VDHICRGGAVRRFVVGPSDDPTELSIASMLA-CQPEPIELPSEHDD-CMISYTSGTT 166 VD C G V S +++ + +A QP P +P DD C+I YTSGTT Sbjct: 122 FEQVD-TCEGIVVLTQSQDESYWNGVITLNAFIADVQPLPDYIPRNADDTCVILYTSGTT 180 Query: 167 GHPKGVITTQANYIVGNGFLNAMYWKLSPDDRIVVTTPLAHRTAFARVGNMAVLG-ATLY 225 G PKG TQ N I+ N + DD +VT PL H N +VLG ATL Sbjct: 181 GLPKGAELTQQN-IIMNALVAQNIMGSQADDIHLVTLPLFHTFGQTVHLNASVLGGATLV 239 Query: 226 IMPRFDVNEAMQLIEAEKISLISIVPTVGRMMLPVIEAEPTRFSSLRVLVATGEAFPAEI 285 ++PRF+ ++L+E ++++ + VPT+ +L V T SSLRV V+ G P EI Sbjct: 240 LVPRFEPKHVLELVEKRRVTIFAGVPTMYIGLLHVQHQFDT--SSLRVAVSGGAPLPTEI 297 Query: 286 KRRLLKALPKLEIFSFFAMTEAGSLAMLKPDEQFDHGASVGRVTPGVEMRLVDADGQTVN 345 R + + I + ++E +A +Q SVG+ GVE+R++D +G + Sbjct: 298 FRTFEETF-NVPILEGYGLSETSPIACFNHLDQERIPGSVGQPVQGVEVRVIDIEGNALP 356 Query: 346 TGEAGEIWVRCGLPGQYLLFRTYFNQPQAMRDAFQEGWFKTGDIGRFDADGFLYIVDRKK 405 GE GEI VR + + + Y ++P+ A Q GWF TGD+GRFD+ G L+IVDR K Sbjct: 357 IGEEGEIVVRG-----HNVMKGYLDRPEVTESALQNGWFHTGDVGRFDSSGNLFIVDRMK 411 Query: 406 DMVLSGGYNIYSKEVEQVLLRYSGVADAAVVGVPDDVYGEAVAAFIEPEVDVMLTEDELI 465 D+++ GG+N+Y +E+E+V + + V+ AV+G+P++ YGE + A++ + + D L Sbjct: 412 DLIIRGGFNVYPREIEEVFMTHPAVSMVAVIGIPNEEYGEEIKAYVVLKPKEYIDADSLQ 471 Query: 466 RWCASELASYKKPKHVVFLRELPRNSTGKVIKTELRT 502 +W +LAS+K P++V LP ++TGK++K EL++ Sbjct: 472 KWGKEQLASFKYPRYVEIREHLPMSATGKILKRELKS 508 >ref|ZP_06181106.1| putative long-chain-fatty-acid-CoA ligase [Vibrio alginolyticus 40B] gb|EEZ82563.1| putative long-chain-fatty-acid-CoA ligase [Vibrio alginolyticus 40B] Length = 538 Score = 297 bits (761), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 182/517 (35%), Positives = 282/517 (54%), Gaps = 25/517 (4%) Query: 1 VKLASYLAAQAQAFPDKAALVCGETRVTFRQLYEDSDRIATALRKSGVAIGDRVAMCLPN 60 + LA+ L A + PDK AL+CG ++ + Q + +IA +L GV GDRVA+ PN Sbjct: 27 MNLAAALQRNAASKPDKTALICGAEKINYAQFDAIAGKIAASLINKGVKPGDRVAISCPN 86 Query: 61 CCEFVTVFFAIVKSGALAMPINMRLSTNEIATIFADAAPAAVFFSEGEREV--------- 111 F V++AI K+GA+A+P+N+ L EI D+ F EG +E+ Sbjct: 87 IPFFAFVYYAIQKAGAVAVPLNVLLKAREIKYHLEDSKAKFYFCFEGTKELPMAKEGVKA 146 Query: 112 ---VDHICRGGAVRRFVVGPSDDPTELSIASMLA-CQPEPIELPSEHDD-CMISYTSGTT 166 VD C G V S +++ + +A QP P +P DD C+I YTSGTT Sbjct: 147 FEQVD-TCEGIVVLTQSQDQSYWNGVITLNAFIADVQPLPDYIPRNADDTCVILYTSGTT 205 Query: 167 GHPKGVITTQANYIVGNGFLNAMYWKLSPDDRIVVTTPLAHRTAFARVGNMAVLG-ATLY 225 G PKG TQ N I+ N + DD +VT PL H N +VLG ATL Sbjct: 206 GLPKGAELTQQN-IIMNALVAQNIMGSQADDIHLVTLPLFHTFGQTVHLNASVLGGATLV 264 Query: 226 IMPRFDVNEAMQLIEAEKISLISIVPTVGRMMLPVIEAEPTRFSSLRVLVATGEAFPAEI 285 ++PRF+ ++L+E ++++ + VPT+ +L V T SSLRV V+ G P EI Sbjct: 265 LVPRFEPKHVLELVEKHRVTIFAGVPTMYIGLLHVQHQFDT--SSLRVAVSGGAPLPTEI 322 Query: 286 KRRLLKALPKLEIFSFFAMTEAGSLAMLKPDEQFDHGASVGRVTPGVEMRLVDADGQTVN 345 R + + I + ++E +A +Q SVG+ GVE++++D +G + Sbjct: 323 FRTFEETF-NVPILEGYGLSETSPIACFNHLDQERIPGSVGQPVQGVEVKVIDIEGNALP 381 Query: 346 TGEAGEIWVRCGLPGQYLLFRTYFNQPQAMRDAFQEGWFKTGDIGRFDADGFLYIVDRKK 405 GE GEI VR + + + Y ++P+ A Q GWF TGD+GRFD+ G L+IVDR K Sbjct: 382 IGEEGEIVVRG-----HNVMKGYLDRPEVTESALQNGWFHTGDVGRFDSSGNLFIVDRMK 436 Query: 406 DMVLSGGYNIYSKEVEQVLLRYSGVADAAVVGVPDDVYGEAVAAFIEPEVDVMLTEDELI 465 D+++ GG+N+Y +E+E+V + + V+ AV+G+P++ YGE + A++ + + D L Sbjct: 437 DLIIRGGFNVYPREIEEVFMTHPAVSMVAVIGIPNEEYGEEIKAYVVLKPKEYIDADSLQ 496 Query: 466 RWCASELASYKKPKHVVFLRELPRNSTGKVIKTELRT 502 +W +LAS+K P++V LP ++TGK++K EL++ Sbjct: 497 KWGKEQLASFKYPRYVEIREHLPMSATGKILKRELKS 533 >ref|YP_703737.1| long-chain-fatty-acid--CoA ligase [Rhodococcus jostii RHA1] gb|ABG95579.1| long-chain-fatty-acid--CoA ligase [Rhodococcus jostii RHA1] Length = 523 Score = 295 bits (755), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 181/525 (34%), Positives = 285/525 (54%), Gaps = 35/525 (6%) Query: 1 VKLASYLAAQAQAFPDKAALVCGETRVTFRQLYEDSDRIATALRKSGVAIGDRVAMCLPN 60 + L+ L A+ FPD+ AL+ G+TR+T+ L S+++A L G+ GD+VA+ PN Sbjct: 2 LNLSVLLEDSARRFPDRDALILGDTRMTYADLDARSNQVANLLMSCGIEPGDKVALSCPN 61 Query: 61 CCEFVTVFFAIVKSGALAMPINMRLSTNEIATIFADAAPAAVFFSEGEREV--------- 111 +F V++ I+K+GA+ +P+N+ L EIA AD+ A +G ++ Sbjct: 62 IPQFPVVYYGILKAGAVVVPLNVLLKDREIAYHLADSDAKAYLCYDGTPDLPMGEYGRSA 121 Query: 112 --VDHICRGGAVRRFVVGPSDDPTEL----SIASMLACQPEPIE--LPSEHDDCMISYTS 163 CR + R P D P + ++A +A + +E + D +I YTS Sbjct: 122 FTQSPHCR--TMFRITADP-DAPATIDGSGTLAEAVAGRDTEVETVVREPGDTAVILYTS 178 Query: 164 GTTGHPKGVITTQANYIVGNGFLNAMYWKLSP--DDRIVVTTPLAHRTAFARVGNMAV-L 220 GTTG PKG T AN ++ N + ++P DR +VT PL H N + + Sbjct: 179 GTTGKPKGAELTHANMVL-NALTANRLFDITPAIHDRYLVTLPLFHSFGQTVTLNAGISV 237 Query: 221 GATLYIMPRFDVNEAMQLIEAEKISLISIVPTVGRMMLPVIEAEPTRF----SSLRVLVA 276 GATL ++PRF+ A+ LIE E I++ + VPT+ +L ++ P ++R+ ++ Sbjct: 238 GATLVLLPRFEAAAALDLIERENITVFAGVPTMYWGLLGALDEHPADIDRIAGNMRLAIS 297 Query: 277 TGEAFPAEIKRRLLKALPKLEIFSFFAMTEAGSLAMLKPDEQFDHGASVGRVTPGVEMRL 336 G A P EI R ++I + ++E LA S+G G+E RL Sbjct: 298 GGAALPVEILTRFADRF-GVQILEGYGLSETSPLATFSDPGGDPRPGSIGVPVWGIEARL 356 Query: 337 VDADGQTVN-TGEAGEIWVRCGLPGQYLLFRTYFNQPQAMRDAFQEGWFKTGDIGRFDAD 395 VD + TV G+ GEI +R + + + Y+N+P A + ++GWF+TGD+ R D D Sbjct: 357 VDRNWNTVTGAGDIGEIALRG-----HNIMKGYYNRPDATAEVLRDGWFRTGDLARIDDD 411 Query: 396 GFLYIVDRKKDMVLSGGYNIYSKEVEQVLLRYSGVADAAVVGVPDDVYGEAVAAFIEPEV 455 GF YIVDR KD+++ GG+N+Y +E+E+VLL + ++ AAVVGVPDD +GE + A++ E Sbjct: 412 GFYYIVDRAKDLIVRGGFNVYPREIEEVLLGHDAISLAAVVGVPDDSHGEEIKAYVILEP 471 Query: 456 DVMLTEDELIRWCASELASYKKPKHVVFLRELPRNSTGKVIKTEL 500 +T DE+I W ++ASYK P+ V F+ LP +TGK++K EL Sbjct: 472 GAKVTADEVIAWAKQQMASYKYPRTVEFVTTLPMTATGKILKREL 516 >ref|ZP_08155872.1| long-chain-fatty-acid-CoA ligase [Rhodococcus equi ATCC 33707] gb|EGD22658.1| long-chain-fatty-acid-CoA ligase [Rhodococcus equi ATCC 33707] Length = 547 Score = 292 bits (747), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 181/517 (35%), Positives = 287/517 (55%), Gaps = 25/517 (4%) Query: 1 VKLASYLAAQAQAFPDKAALVCGETRVTFRQLYEDSDRIATALRKSGVAIGDRVAMCLPN 60 + L+ +L A+ +P + ALV G++R T+ + +++IA L SG+ GDRVA+ PN Sbjct: 38 LNLSMFLEDSARKYPQRDALVLGDSRFTYAETDGLANQIANLLVASGIEPGDRVAISCPN 97 Query: 61 CCEFVTVFFAIVKSGALAMPINMRLSTNEIATIFADAAPAAVFFSEG--EREVVDHICRG 118 F V++ I+K+GA+ +P+N+ L EI D AA F EG E + ++ G Sbjct: 98 VPWFPIVYYGILKAGAVVVPLNVLLKGREITYHLQDCGAAAYFCFEGGPELPIGEYGREG 157 Query: 119 -----GAVRRFVVGPSDDPTELSIASMLACQPEPIELPS--EHDDCMISYTSGTTGHPKG 171 G FV+ S S++ +L QP E + D +I YTSGTTG PKG Sbjct: 158 FRSAEGCHAMFVIDGSSGDGVTSLSDVLPAQPNTFEAAACGATDTAVILYTSGTTGQPKG 217 Query: 172 VITTQANYIVGNGFLNAMYWKL-SPDDRIVVTTPLAHRTAFARVGNMAV---LGATLYIM 227 T +N ++ N ++ S D ++T PL H +F + NM +GATL ++ Sbjct: 218 AELTHSNMVLNAIAANRLFDSTPSVPDTHLLTLPLFH--SFGQTINMNAGFSVGATLVLL 275 Query: 228 PRFDVNEAMQLIEAEKISLISIVPTVGRMMLPVIE--AEPTRFS-SLRVLVATGEAFPAE 284 PRF+ A++++E E I++ + VPT+ +L ++ + R + +LR ++ G A P E Sbjct: 276 PRFEAGAALEIMEREAITMFAGVPTMYWSLLGAVDDSVDVERIAGNLRKAISGGAALPVE 335 Query: 285 IKRRLLKALPKLEIFSFFAMTEAGSLAMLKPDEQFDHGASVGRVTPGVEMRLVDADGQTV 344 I R ++I + ++E +A+ EQ S+G GV++RLVD D TV Sbjct: 336 ILERFASRF-GVQILEGYGLSETSPVALFSDPEQAPRPGSIGVPLWGVQVRLVDRDWNTV 394 Query: 345 NTGEA-GEIWVRCGLPGQYLLFRTYFNQPQAMRDAFQEGWFKTGDIGRFDADGFLYIVDR 403 + GEI +R + + + Y +P A +A ++GWF+TGD+ R DADGF YIVDR Sbjct: 395 EGADVVGEIAIRG-----HNVMKGYLGRPDATAEAMRDGWFRTGDLARRDADGFYYIVDR 449 Query: 404 KKDMVLSGGYNIYSKEVEQVLLRYSGVADAAVVGVPDDVYGEAVAAFIEPEVDVMLTEDE 463 KDM++ GG+N+Y +EVE+VL+ + V+ AAVVGVP + +GE V A++ P ++ + Sbjct: 450 AKDMIVRGGFNVYPREVEEVLMTHDAVSLAAVVGVPHERHGEEVRAYVIPAAGTEVSAAD 509 Query: 464 LIRWCASELASYKKPKHVVFLRELPRNSTGKVIKTEL 500 L+ W +ASYK P+ V F+ +LP +TGK++K EL Sbjct: 510 LVAWSKERMASYKYPREVHFVEQLPMTATGKILKREL 546