BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0017.p (320 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_486715.1| esterase/lipase/thioesterase [Rhodopseudomonas ... 449 e-124 ref|YP_559193.1| putative esterase/lipase/thioesterase [Burkhold... 237 2e-60 ref|YP_002944669.1| alpha/beta hydrolase fold-3 domain-containin... 211 1e-52 ref|YP_587329.1| lipase/esterase [Cupriavidus metallidurans CH34... 210 3e-52 ref|YP_001895120.1| alpha/beta hydrolase fold-3 domain-containin... 201 1e-49 >ref|YP_486715.1| esterase/lipase/thioesterase [Rhodopseudomonas palustris HaA2] gb|ABD07804.1| Esterase/lipase/thioesterase [Rhodopseudomonas palustris HaA2] Length = 305 Score = 449 bits (1155), Expect = e-124, Method: Compositional matrix adjust. Identities = 212/285 (74%), Positives = 244/285 (85%), Gaps = 1/285 (0%) Query: 5 PEIPSDLRVLMAEIGPKWASDTTGHVRLMVEEFSRVLKSSPKDGIQAE-TVRYGTHERQA 63 P+IP++LR LMAEIGPKWA++T GH++LM++EFS +LK SPK G+ E + YG HERQA Sbjct: 7 PDIPAELRALMAEIGPKWATNTKGHIQLMIDEFSELLKHSPKQGVDVEPNITYGPHERQA 66 Query: 64 FEVFQPADGSSTTRPGLIFVHGGAFTEGSRHRSTEIYANVLYYFARHGIVGINAGYRLAP 123 F+V++P S T RPGLIFVHGGAFTEG R RS+EIYANVLYYFARHGIVGINAGYRLAP Sbjct: 67 FDVYRPHSPSQTPRPGLIFVHGGAFTEGRRDRSSEIYANVLYYFARHGIVGINAGYRLAP 126 Query: 124 DARYPEATRDIAAIVHWMRNNAANLGVDPNRIFLMGHSAGGAHVGSYAYDRRHQPATGHG 183 +ARYP ATRDIA IV WMR NA +LGVDP RIFLMGHSAGGAHVGSYA+DRRHQ GHG Sbjct: 127 EARYPGATRDIAKIVAWMRANATDLGVDPERIFLMGHSAGGAHVGSYAFDRRHQADGGHG 186 Query: 184 LAGVLIVSGRVRADARADNPNAKRVTEYYGPDVSTYDDVSPVSHVDSSSPPTFVACSEFE 243 LAGVLI+SGRVRAD+ A NPNA RV EYYG D + ++DVSPV+HVD++SPPTF+AC+EFE Sbjct: 187 LAGVLIISGRVRADSHAHNPNALRVAEYYGSDAAVHEDVSPVNHVDATSPPTFIACAEFE 246 Query: 244 NPLIDVYCFELAHRLGAAKGHAPPFAWMKGHNHMSIIAHFDTAED 288 NPLIDVYCFELA+RL AAK APPF W+KGHNH SII F+TAED Sbjct: 247 NPLIDVYCFELAYRLAAAKRKAPPFMWLKGHNHTSIIGQFNTAED 291 >ref|YP_559193.1| putative esterase/lipase/thioesterase [Burkholderia xenovorans LB400] gb|ABE31141.1| Putative esterase/lipase/thioesterase [Burkholderia xenovorans LB400] Length = 328 Score = 237 bits (604), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 124/295 (42%), Positives = 178/295 (60%), Gaps = 17/295 (5%) Query: 7 IPSDLRVLMAEIGPKWASDTTGHVRLMVEEFSRVLKSSPKDGIQAE-TVRYGTHERQAFE 65 +P D R LM +IGP+W D GH +VE ++ V+ ++PKDGI E VRYG HER + Sbjct: 14 LPEDQRRLMLDIGPRWGEDILGHRLKVVECYTPVVAAAPKDGIVVERDVRYGPHERHVLD 73 Query: 66 VFQPADGSSTTRPG-------LIFVHGGAFTEGSRHRSTEIYANVLYYFARHGIVGINAG 118 F PA + T + G ++FVHGGAF G++ + EIY NV Y+FA G+V IN Sbjct: 74 TFLPARSTETVQAGHGRPLDAVVFVHGGAFVRGNKSVNGEIYDNVCYWFASQGLVAINVE 133 Query: 119 YRLAPDARYPEATRDIAAIVHWMRNNAANLGVDPNRIFLMGHSAGGAHVGSYAYDRRHQP 178 YRLA A YPE RD+ + ++ NA L +DP+R+F++GHSAGGAH + +D P Sbjct: 134 YRLADAAPYPEGARDVCDALAFIHRNAERLSIDPHRVFVIGHSAGGAHAATCLFD----P 189 Query: 179 ATGHG-----LAGVLIVSGRVRADARADNPNAKRVTEYYGPDVSTYDDVSPVSHVDSSSP 233 A +AG++++S R+ AD R+ NPNAK V Y+G D + Y + SP++HV S+ Sbjct: 190 AITEKTPPPPVAGLVLISARLVADVRSGNPNAKPVRAYFGDDETLYPERSPLAHVARSTV 249 Query: 234 PTFVACSEFENPLIDVYCFELAHRLGAAKGHAPPFAWMKGHNHMSIIAHFDTAED 288 P +A +EFENP +D Y + +G P ++GHNH SI+AHF++ E+ Sbjct: 250 PLMIAIAEFENPYLDEYGAQFFVEALRHRGVPPRLIQLRGHNHTSIVAHFNSGEE 304 >ref|YP_002944669.1| alpha/beta hydrolase fold-3 domain-containing protein [Variovorax paradoxus S110] gb|ACS19403.1| Alpha/beta hydrolase fold-3 domain protein [Variovorax paradoxus S110] Length = 301 Score = 211 bits (538), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 111/284 (39%), Positives = 168/284 (59%), Gaps = 10/284 (3%) Query: 8 PSDLRVLMAEIGPKWASDTTGHVRLMVEEFSRVLKSSPKDGIQA-ETVRYGTHERQAFEV 66 P+ + L+ E+GP W D G + + VL ++P G+ + YG+H RQ +V Sbjct: 7 PAGMDELLREVGPVWQQDIRGAGDRVKAAYLPVLMAAPHGGVDVTRDLSYGSHARQRLDV 66 Query: 67 FQPADGSSTTRPGLIFVHGGAFTEGSRHRSTEIYANVLYYFARHGIVGINAGYRLAPDAR 126 + PA + P ++FVHGGAF G++ + +YANVL +FAR G VG+N YRLAP+A Sbjct: 67 YCPA--VARDAPVVVFVHGGAFVRGTKDINDAMYANVLTWFARQGCVGVNVEYRLAPEAP 124 Query: 127 YPEATRDIAAIVHWMRNNAANLGVDPNRIFLMGHSAGGAHVGSYAYDRRHQPATGH---G 183 +P+ D+AA W+ + G DP I L+GHSAGG H ++A D PA H Sbjct: 125 HPQGALDVAAACAWVSAHIGGFGGDPRSICLIGHSAGGTHAATFACD----PALAHLRRD 180 Query: 184 LAGVLIVSGRVRADARADNPNAKRVTEYYGPDVSTYDDVSPVSHVDSSSPPTFVACSEFE 243 + +++VS R++AD+ +NPNA V Y+G D S Y +SP++H + P FV +++E Sbjct: 181 VCCLVLVSARLQADSLPENPNAAGVRAYFGEDASRYPSLSPMAHAAHLAMPVFVVNAQYE 240 Query: 244 NPLIDVYCFELAHRLGAAKGHAPPFAWMKGHNHMSIIAHFDTAE 287 NPL+DVY E A+R+ A+ AP + HNH+S++AHF++ E Sbjct: 241 NPLLDVYATEFAYRVAEARRVAPRHMTVADHNHVSVMAHFNSGE 284 >ref|YP_587329.1| lipase/esterase [Cupriavidus metallidurans CH34] gb|ABF12060.1| lipase/esterase [Cupriavidus metallidurans CH34] Length = 310 Score = 210 bits (534), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 118/289 (40%), Positives = 164/289 (56%), Gaps = 11/289 (3%) Query: 5 PEIPSDLRVLMAEIGPKWASDTTGHVRLMVEEFSRVLKSSPKDGIQAE-TVRYGTHERQA 63 P + S M +GP+W D + R + + V + P DGI++ + YGTH RQ Sbjct: 8 PGLSSSQASAMLALGPRWTDDIQANRRQVCTIYDSVHAAQPADGIRSHANIAYGTHARQR 67 Query: 64 FEVFQPADGSST--TRPGLIFVHGGAFTEGSRHRSTEIYANVLYYFARHGIVGINAGYRL 121 +++ P +GS+ RP ++FVHGGAF G + S IYANV + AR G +G+N YRL Sbjct: 68 LDLYLP-EGSAAGDDRPVVVFVHGGAFIRGDKDASPHIYANVPRWCARQGYIGVNVEYRL 126 Query: 122 APDARYPEATRDIAAIVHWMRNNAANLGVDPNRIFLMGHSAGGAHVGSYAYDRRHQPATG 181 AP+A YP D+A + W+R +A G DP RI L+GHSAGG+HV S D PA G Sbjct: 127 APEASYPAGAEDVAMALAWLRAHARAHGGDPGRIVLIGHSAGGSHVASCLSD----PACG 182 Query: 182 H---GLAGVLIVSGRVRADARADNPNAKRVTEYYGPDVSTYDDVSPVSHVDSSSPPTFVA 238 G+AG ++VS R++AD DNPNA V YYG D + +P+ P VA Sbjct: 183 ENGSGVAGAVLVSARLQADTLPDNPNANGVAAYYGSDPALQLAHAPMPFAGRLRVPLMVA 242 Query: 239 CSEFENPLIDVYCFELAHRLGAAKGHAPPFAWMKGHNHMSIIAHFDTAE 287 +EFENP +D+Y + A R+ A+G +P + HNH SI+AH TA+ Sbjct: 243 FAEFENPYLDLYALQYAARVREAQGRSPRVVQVARHNHTSIVAHLGTAD 291 >ref|YP_001895120.1| alpha/beta hydrolase fold-3 domain-containing protein [Burkholderia phytofirmans PsJN] gb|ACD15896.1| Alpha/beta hydrolase fold-3 domain protein [Burkholderia phytofirmans PsJN] Length = 318 Score = 201 bits (512), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 120/302 (39%), Positives = 162/302 (53%), Gaps = 28/302 (9%) Query: 7 IPSDLRVLMAEIGPKWASDTTGHVRLMVEEFSRVLKSSPKDGIQAETVR---YGTHERQA 63 + + LR A+IGP W D H L+++ +S ++ ++ G E R YG HERQ Sbjct: 11 LSAALRQRFAQIGPVWGRDINRHRDLVIDAYSPLVTAARDAGESFEVARELAYGGHERQR 70 Query: 64 FEVFQPADGSSTTRPG----LIFVHGGAFTEGSRHRSTEIYANVLYYFARHGIVGINAGY 119 +VF + T R G ++FVHGGAF GS+ + IY NV +FAR G V +N Y Sbjct: 71 LDVFASEE---TRRKGNADVVLFVHGGAFLRGSKSFNGLIYDNVSRWFARQGCVALNVEY 127 Query: 120 RLAPDARYPEATRDIAAIVHWMRNNAANLGVDPNRIFLMGHSAGGAHVGSYAYD------ 173 RLAPDA YP D+AA + W + + G +P RIFL+GHSAGGAHV +Y D Sbjct: 128 RLAPDAPYPAGADDVAAALAWAQRHVGEFGGNPQRIFLIGHSAGGAHVATYLCDPLFSEL 187 Query: 174 RRHQPATGHGLAGVLIVSGRVRADARADNPNAKRVTEYYGPDVSTYDDVSPVSHVDSSSP 233 R P +AG +++S R+ AD ADNPNA V Y+G D + Y SP++H Sbjct: 188 RDSMPR----VAGAVLISARLVADVHADNPNAVGVRAYFGADPTRYAARSPLTHAAHFDT 243 Query: 234 PTFVACSEFENPLIDVYCFELAHRL--------GAAKGHAPPFAWMKGHNHMSIIAHFDT 285 P V +E+ENP +D Y R+ AA AP F M HNH S++AHFD+ Sbjct: 244 PLMVVVAEYENPYLDAYGAAFFERVLTARRSAGEAAAQRAPRFVQMLRHNHTSVVAHFDS 303 Query: 286 AE 287 E Sbjct: 304 GE 305