BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0016.p (286 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_486714.1| amidohydrolase 2 [Rhodopseudomonas palustris Ha... 499 e-139 ref|ZP_07966262.1| amidohydrolase [Segniliparus rugosus ATCC BAA... 243 2e-62 ref|YP_004210325.1| amidohydrolase [Acidobacterium sp. MP5ACTX9]... 230 2e-58 ref|ZP_07030062.1| amidohydrolase 2 [Acidobacterium sp. MP5ACTX8... 230 2e-58 ref|YP_889608.1| amidohydrolase 2 [Mycobacterium smegmatis str. ... 225 5e-57 >ref|YP_486714.1| amidohydrolase 2 [Rhodopseudomonas palustris HaA2] gb|ABD07803.1| Amidohydrolase 2 [Rhodopseudomonas palustris HaA2] Length = 317 Score = 499 bits (1284), Expect = e-139, Method: Compositional matrix adjust. Identities = 238/282 (84%), Positives = 258/282 (91%) Query: 5 LAEIGDTRLRAMDEAGIDVQVLSHGAPAAQKLRPEIAVDLTKRVNDRLAEACARHPGRFA 64 L E+GD RLRAMDEAGIDVQVLSHGAPAAQKL P++A +LT+RVNDRLAEACAR P RFA Sbjct: 36 LTELGDVRLRAMDEAGIDVQVLSHGAPAAQKLPPDVAPELTRRVNDRLAEACARFPNRFA 95 Query: 65 AFAALPTPVPEQAARELERCVNELKFKGAMIHGLTNGLFIDDKRFWPIFEVAEKLDVPIY 124 AFAALPTP PE AARELERCV +L FKGAMIHGLTNGLFIDDKRFWPIFEVAEKLDVPIY Sbjct: 96 AFAALPTPAPEAAARELERCVRDLGFKGAMIHGLTNGLFIDDKRFWPIFEVAEKLDVPIY 155 Query: 125 LHPSVPHSEVTKYYYEDYARDFPTVIRPAWGFTVETATAAIRLVLSRALEKYPRLQIILG 184 LHPSVPH++VT+YYY+DYAR+FPTVIRPAWGFTVETAT AIRL+LSRALE YPRLQIILG Sbjct: 156 LHPSVPHADVTRYYYDDYAREFPTVIRPAWGFTVETATQAIRLILSRALESYPRLQIILG 215 Query: 185 HFGETLPFLLWRIDQALARPGQEGLGFRRQFSQHFHVTCSGFFSTPALTCTMLELGIDRI 244 H GETLPFLLWRI+QALARPGQ L FR+QF HFHVT SGFFSTPAL CTMLELGIDRI Sbjct: 216 HLGETLPFLLWRINQALARPGQTPLDFRKQFCDHFHVTTSGFFSTPALICTMLELGIDRI 275 Query: 245 MFAVDYPYVDNKDGMNWIRELQLSIDDKRKLVGNNARRLLKL 286 MFAVDYPYV N+DG +W+++LQ+S DDKRKLVG NARRLLKL Sbjct: 276 MFAVDYPYVVNRDGTDWVQQLQISPDDKRKLVGGNARRLLKL 317 >ref|ZP_07966262.1| amidohydrolase [Segniliparus rugosus ATCC BAA-974] gb|EFV12506.1| amidohydrolase [Segniliparus rugosus ATCC BAA-974] Length = 338 Score = 243 bits (621), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 124/281 (44%), Positives = 172/281 (61%), Gaps = 1/281 (0%) Query: 7 EIGDTRLRAMDEAGIDVQVLSHGAPAAQKLRPEIAVDLTKRVNDRLAEACARHPGRFAAF 66 +IG R+ AMDEAGIDVQVLS P + L AV L K ND +A A HP RF F Sbjct: 55 DIGAGRIAAMDEAGIDVQVLSQTTPGVEMLEAGPAVRLAKEANDAIAGAVEAHPARFRGF 114 Query: 67 AALPTPVPEQAARELERCVNELKFKGAMIHGLTNGLFIDDKRFWPIFEVAEKLDVPIYLH 126 A LP P +AARELER V +L F GAM++G G ++DD+++WPIF AE+L VP+YLH Sbjct: 115 AHLPVQDPAEAARELERAVTQLGFVGAMLNGHVRGEYLDDQKYWPIFAKAEELGVPVYLH 174 Query: 127 PSVPHSEVTKYYYEDYARDFPTVI-RPAWGFTVETATAAIRLVLSRALEKYPRLQIILGH 185 P+ P V Y ++ ++ PA+G+ V+ A+RL+L+ +++P LQ+ILGH Sbjct: 175 PTRPPQAVVDAQYAGFSPAVTEMLAAPAFGWHVDAGLHALRLILAGLFDRHPGLQMILGH 234 Query: 186 FGETLPFLLWRIDQALARPGQEGLGFRRQFSQHFHVTCSGFFSTPALTCTMLELGIDRIM 245 GETLPF++ R + LA + + F+ HF +T +GFF+ P LG DRI+ Sbjct: 235 QGETLPFMIDRASERLASVVRLSTSIKEAFASHFSITFAGFFTYPPFVAAYHCLGADRIL 294 Query: 246 FAVDYPYVDNKDGMNWIRELQLSIDDKRKLVGNNARRLLKL 286 F+VDYPY N DG+ ++ L +S DD+ K+ NA RLL + Sbjct: 295 FSVDYPYSRNADGVAYLDRLPISADDREKIAHRNAERLLGI 335 >ref|YP_004210325.1| amidohydrolase [Acidobacterium sp. MP5ACTX9] gb|ADW71007.1| amidohydrolase 2 [Acidobacterium sp. MP5ACTX9] Length = 322 Score = 230 bits (586), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 121/286 (42%), Positives = 171/286 (59%), Gaps = 3/286 (1%) Query: 4 QLAEIGDTRLRAMDEAGIDVQVLSHGAPAAQKLRPEIAVDLTKRVNDRLAEACARHPGRF 63 +L ++GD R+ AMDEAGID+QVLS A L A L +ND LA A +P R Sbjct: 36 KLLDLGDGRIAAMDEAGIDLQVLSLAALGFDALDAATATPLVHDINDELAAAVRANPTRL 95 Query: 64 AAFAALPTPVPEQAARELERCVNELKFKGAMIHGLTNGLFIDDKRFWPIFEVAEKLDVPI 123 AAFA+L P+ AARELER + +L F+G ++ G T+GLF+DD RF P+FE A L+VPI Sbjct: 96 AAFASLALKDPQSAARELERAIQKLGFRGVLLDGTTDGLFLDDPRFTPVFEAAVALNVPI 155 Query: 124 YLHPSVPHSEVTKYYYEDYARDFPTVIRPA-WGFTVETATAAIRLVLSRALEKYPRLQII 182 YLHP+ P V Y+ ++ A WG+ ETA +RL+ + ++YP LQ+I Sbjct: 156 YLHPAPPPESVFDTYFTGLPEGVGQMLSIAGWGWHAETALHTLRLITNGVFDRYPTLQLI 215 Query: 183 LGHFGETLPFLLWRIDQALARPGQEGLGFRRQFSQHFHVTCSGFFSTPALTCTMLELGID 242 +GH GE LP L R +AL+ + F + H+T SG+F+ P L C + +GID Sbjct: 216 IGHMGEMLPMALARTSKALSHAARLRQPVAAYFQSNIHLTTSGYFTQPPLRCALDVVGID 275 Query: 243 RIMFAVDYPYVDNKDGMNWIRELQ--LSIDDKRKLVGNNARRLLKL 286 R+MF++DYP+ N G +++ EL+ L+ +D KL+ NA LL L Sbjct: 276 RLMFSIDYPFSANTLGRDYLTELEQTLTPEDLAKLIHRNAESLLNL 321 >ref|ZP_07030062.1| amidohydrolase 2 [Acidobacterium sp. MP5ACTX8] gb|EFI57549.1| amidohydrolase 2 [Acidobacterium sp. MP5ACTX8] Length = 338 Score = 230 bits (586), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 126/290 (43%), Positives = 169/290 (58%), Gaps = 10/290 (3%) Query: 4 QLAEIGDTRLRAMDEAGIDVQVLSHGAPAAQKLRPEIAVDLTKRVNDRLAEACARHPGRF 63 +L ++G R+ AMDEA +D QVLS A L A L + VND LAEA HP R Sbjct: 52 KLLDLGAGRIAAMDEAAVDFQVLSLAAMGFDSLDAATANALAQDVNDELAEAVRAHPSRL 111 Query: 64 AAFAALPTPVPEQAARELERCVNELKFKGAMIHGLTNGLFIDDKRFWPIFEVAEKLDVPI 123 FA L P+ AA ELERCV +L F+GA++ G T+GLF+DD RF P+FE A L VP+ Sbjct: 112 GGFATLALKDPDTAAVELERCVTQLGFRGALVDGTTDGLFLDDPRFLPVFEAAAHLGVPV 171 Query: 124 YLHPSVPHSEVTKYYYEDYARDFPTVIRPA-WGFTVETATAAIRLVLSRALEKYPRLQII 182 YLHP++P V Y+ + ++ A WG+ ET +RL+LS +++P LQ+I Sbjct: 172 YLHPALPPEPVKNAYFSGLPGELGHLLSIAGWGWHAETGLHTLRLILSGLFDRFPSLQLI 231 Query: 183 LGHFGETLPFLLWR----IDQALARPGQEGLGFRRQFSQHFHVTCSGFFSTPALTCTMLE 238 +GH GE LP+ L R + QA Q G+ F + H+T SG+FS P L C M Sbjct: 232 IGHMGEGLPYALARSSGVLSQAAPHLRQPVAGY---FKSNIHLTTSGYFSQPPLRCAMDV 288 Query: 239 LGIDRIMFAVDYPYVDNKDGMNWIRELQ--LSIDDKRKLVGNNARRLLKL 286 +GIDR+MF++DYP+ N G ++ LQ LS +D KLV NA LL L Sbjct: 289 VGIDRLMFSIDYPFSPNGRGRAYLDSLQELLSSEDLAKLVHRNAEALLGL 338 >ref|YP_889608.1| amidohydrolase 2 [Mycobacterium smegmatis str. MC2 155] gb|ABK73921.1| amidohydrolase 2 [Mycobacterium smegmatis str. MC2 155] Length = 317 Score = 225 bits (574), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 114/284 (40%), Positives = 162/284 (57%), Gaps = 1/284 (0%) Query: 4 QLAEIGDTRLRAMDEAGIDVQVLSHGAPAAQKLRPEIAVDLTKRVNDRLAEACARHPGRF 63 +L +G+ RLR MD+AGID+QVLS AP Q++ PE A DL++ +NDR AE A HP RF Sbjct: 34 KLTAVGEQRLRVMDDAGIDLQVLSVTAPGTQQVDPEYAGDLSRTLNDRCAEIVAAHPDRF 93 Query: 64 AAFAALPTPVPEQAARELERCVNELKFKGAMIHGLTNGLFIDDKRFWPIFEVAEKLDVPI 123 A A+LPT +P A E +R + EL G +I+G T G F+D F + E+L VP+ Sbjct: 94 NALASLPTQIPTAAVVEAKRAITELGMCGVVINGHTQGTFLDAPEFDELLSAIEELGVPV 153 Query: 124 YLHPSVPHSEVTKYYYEDYARDFPTVI-RPAWGFTVETATAAIRLVLSRALEKYPRLQII 182 YLHP+ P ++V + Y+ + + + AWG+ ET +RL S +++P LQ + Sbjct: 154 YLHPTYPPAQVAEVYFGGLTPEIGSALATAAWGWHAETGLHVLRLAASGVFDRHPALQFV 213 Query: 183 LGHFGETLPFLLWRIDQALARPGQEGLGFRRQFSQHFHVTCSGFFSTPALTCTMLELGID 242 +GH GE LPF L R D L+ G H H+T SG+ + P L C + G D Sbjct: 214 VGHMGENLPFSLMRADAVLSGYNPGGPSVAETVRAHVHITISGYTTAPPLLCALQVFGAD 273 Query: 243 RIMFAVDYPYVDNKDGMNWIRELQLSIDDKRKLVGNNARRLLKL 286 RIMFA DYPY D ++ + +S D+ K+ NAR+L +L Sbjct: 274 RIMFAADYPYGDAVTHAEFLAQAPISPADREKIAHVNARQLFRL 317