BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0023.p (509 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_767149.1| malonyl-CoA synthase [Bradyrhizobium japonicum ... 913 0.0 ref|YP_001236428.1| malonyl-CoA synthase [Bradyrhizobium sp. BTA... 913 0.0 ref|YP_001202443.1| malonyl-CoA synthase [Bradyrhizobium sp. ORS... 898 0.0 ref|YP_578788.1| malonyl-CoA synthase [Nitrobacter hamburgensis ... 872 0.0 ref|ZP_08627984.1| long-chain-fatty-acid--CoA ligase [Bradyrhizo... 854 0.0 >ref|NP_767149.1| malonyl-CoA synthase [Bradyrhizobium japonicum USDA 110] gb|AAF28840.1|AF118888_1 malonyl CoA synthetase [Bradyrhizobium japonicum] dbj|BAC45774.1| malonyl CoA synthetase [Bradyrhizobium japonicum USDA 110] Length = 509 Score = 913 bits (2359), Expect = 0.0, Method: Compositional matrix adjust. Identities = 442/508 (87%), Positives = 471/508 (92%) Query: 1 MNTTTNANLFSRLFDGLDDPGRLAIEQLDGSRISYGDLIARAGQMANVLVERGVKPGDRV 60 MN NANLFSRLFDGLDDP RLAIE DG+RISYGDLIARAGQMANVLV RGVKPGDRV Sbjct: 1 MNRAANANLFSRLFDGLDDPKRLAIETHDGARISYGDLIARAGQMANVLVARGVKPGDRV 60 Query: 61 AAQTEKSVSALVLYLATVRAGGVYLPLNTAYTLNELEYFITDAEPALVVCDPSKQDGIKA 120 A Q EKSV+ +VLYLATVRAG VYLPLNTAYTLNEL+YFI DAEP+LVVCDPSK +G+ Sbjct: 61 AVQVEKSVANIVLYLATVRAGAVYLPLNTAYTLNELDYFIGDAEPSLVVCDPSKAEGLAP 120 Query: 121 IAAKVGAKVETLDASGKGSLTEAADKAAKEFATVPRGNNDLAAILYTSGTTGRSKGAMLS 180 IAAKV A VETL GKGSLTEAADKA+ F TVPR N+DLAAILYTSGTTGRSKGAML+ Sbjct: 121 IAAKVKAGVETLGPDGKGSLTEAADKASSAFTTVPRENDDLAAILYTSGTTGRSKGAMLT 180 Query: 181 HDNLASNSYSLVDYWRFSDKDVLIHALPIYHTHGLFVASNVTLFARASMIFLPKFDPDVI 240 HDNLASNS SLV YWRF+DKDVLIHALPIYHTHGLFVA+NVTLF+RASMIFLPK DPD+I Sbjct: 181 HDNLASNSLSLVGYWRFTDKDVLIHALPIYHTHGLFVATNVTLFSRASMIFLPKLDPDLI 240 Query: 241 IKLMARATVMMGVPTFYTRLLQNPGLNKDATGHMRLFISGSAPLLADTHREWSARTGHAV 300 IKLMARATV+MGVPTFYTRLLQN L+++ T HMRLFISGSAPLLA+THREWSARTGHAV Sbjct: 241 IKLMARATVLMGVPTFYTRLLQNAALSRETTRHMRLFISGSAPLLAETHREWSARTGHAV 300 Query: 301 LERYGMTETNMNTSNPYDGDRVPGAVGFALPGVSVRVTDPESGKELPRETIGMIEVKGPN 360 LERYGMTETNMNTSNPYDG+RVPGAVGF LPGVS+RVTDPE+GKELPRE IGMIEVKGPN Sbjct: 301 LERYGMTETNMNTSNPYDGERVPGAVGFPLPGVSLRVTDPETGKELPREEIGMIEVKGPN 360 Query: 361 VFQGYWRMPEKTKAEFRGDGFFITGDLGKIDDNGYVHILGRGKDLVISGGFNVYPKEIES 420 VF+GYWRMPEKTKAEFR DGFFITGDLGKID GYVHILGRGKDLVISGGFNVYPKEIES Sbjct: 361 VFKGYWRMPEKTKAEFRPDGFFITGDLGKIDGKGYVHILGRGKDLVISGGFNVYPKEIES 420 Query: 421 EIDAMPGVIESAVIGVPHADFGEGVTAVLVCSKGADVSEASVLKALDGRLAKFKMPKRVF 480 EIDAMPGV+ESAVIGVPHADFGEGVTAVLVC+KGA+VSEASVLKALDGRLAKFKMPKRVF Sbjct: 421 EIDAMPGVVESAVIGVPHADFGEGVTAVLVCNKGAEVSEASVLKALDGRLAKFKMPKRVF 480 Query: 481 VVEELPRNAMGKVQKNILRDTYKNIYAK 508 VV+ELPRN MGKVQKN+LRDTYK+IYAK Sbjct: 481 VVDELPRNTMGKVQKNVLRDTYKDIYAK 508 >ref|YP_001236428.1| malonyl-CoA synthase [Bradyrhizobium sp. BTAi1] gb|ABQ32522.1| putative O-succinylbenzoate--CoA ligase (menE) [Bradyrhizobium sp. BTAi1] Length = 508 Score = 913 bits (2359), Expect = 0.0, Method: Compositional matrix adjust. Identities = 441/508 (86%), Positives = 469/508 (92%) Query: 1 MNTTTNANLFSRLFDGLDDPGRLAIEQLDGSRISYGDLIARAGQMANVLVERGVKPGDRV 60 MN T NANLF+RLFDGLDDP RLAIE DG RI+YGDLIARAGQMANVLV RGVKPGDRV Sbjct: 1 MNQTANANLFARLFDGLDDPSRLAIETHDGQRITYGDLIARAGQMANVLVSRGVKPGDRV 60 Query: 61 AAQTEKSVSALVLYLATVRAGGVYLPLNTAYTLNELEYFITDAEPALVVCDPSKQDGIKA 120 AAQTEKSVS LVLYLATVRAGGVYLPLNTAYTLNEL+YFI DAEP +VVCDP+K +GI+ Sbjct: 61 AAQTEKSVSGLVLYLATVRAGGVYLPLNTAYTLNELDYFIGDAEPTVVVCDPAKAEGIRT 120 Query: 121 IAAKVGAKVETLDASGKGSLTEAADKAAKEFATVPRGNNDLAAILYTSGTTGRSKGAMLS 180 +AAKVGA V+TLDASGKGSLTEAADKAA F TVPRG +DLAAILYTSGTTGRSKGAMLS Sbjct: 121 LAAKVGATVDTLDASGKGSLTEAADKAATAFTTVPRGADDLAAILYTSGTTGRSKGAMLS 180 Query: 181 HDNLASNSYSLVDYWRFSDKDVLIHALPIYHTHGLFVASNVTLFARASMIFLPKFDPDVI 240 HDNLASNS +L+DYWRF+ DVLIHALPIYHTHGLFVASNVTLFARASMIFLPK DPD+I Sbjct: 181 HDNLASNSLTLIDYWRFTKDDVLIHALPIYHTHGLFVASNVTLFARASMIFLPKLDPDLI 240 Query: 241 IKLMARATVMMGVPTFYTRLLQNPGLNKDATGHMRLFISGSAPLLADTHREWSARTGHAV 300 I LMARATV+MGVPTFYTRLLQNP LNK+ T HMRLFISGSAPLLADTHREW ARTGHAV Sbjct: 241 INLMARATVLMGVPTFYTRLLQNPRLNKETTSHMRLFISGSAPLLADTHREWFARTGHAV 300 Query: 301 LERYGMTETNMNTSNPYDGDRVPGAVGFALPGVSVRVTDPESGKELPRETIGMIEVKGPN 360 LERYGMTETNMNTSNPYDG+RVPGAVGF LPGVSVRVTDPE+GKEL R+ IGMIEVKGPN Sbjct: 301 LERYGMTETNMNTSNPYDGERVPGAVGFPLPGVSVRVTDPETGKELARDEIGMIEVKGPN 360 Query: 361 VFQGYWRMPEKTKAEFRGDGFFITGDLGKIDDNGYVHILGRGKDLVISGGFNVYPKEIES 420 VF+GYWRMPEKTK+EFR DGFFITGDLGKID GYVHI+GRGKDLVISGGFNVYPKEIES Sbjct: 361 VFKGYWRMPEKTKSEFRPDGFFITGDLGKIDTQGYVHIVGRGKDLVISGGFNVYPKEIES 420 Query: 421 EIDAMPGVIESAVIGVPHADFGEGVTAVLVCSKGADVSEASVLKALDGRLAKFKMPKRVF 480 EIDAMPGV+ESAVIGVPHADFGEGVTAV+V GADV+EASVLK LDGRLAKFKMPKRVF Sbjct: 421 EIDAMPGVVESAVIGVPHADFGEGVTAVVVRHPGADVNEASVLKGLDGRLAKFKMPKRVF 480 Query: 481 VVEELPRNAMGKVQKNILRDTYKNIYAK 508 VV+ELPRN MGKVQKN+LRD YK+IY K Sbjct: 481 VVDELPRNTMGKVQKNVLRDQYKDIYTK 508 >ref|YP_001202443.1| malonyl-CoA synthase [Bradyrhizobium sp. ORS 278] emb|CAL74193.1| putative O-succinylbenzoate--CoA ligase (menE) [Bradyrhizobium sp. ORS 278] Length = 508 Score = 898 bits (2321), Expect = 0.0, Method: Compositional matrix adjust. Identities = 435/508 (85%), Positives = 463/508 (91%) Query: 1 MNTTTNANLFSRLFDGLDDPGRLAIEQLDGSRISYGDLIARAGQMANVLVERGVKPGDRV 60 MN N NLF+RLFDGLDDP RLAIE DG RI+YGDL+ARAGQMANVLV RGVKPGDRV Sbjct: 1 MNHAANTNLFARLFDGLDDPSRLAIETHDGQRITYGDLVARAGQMANVLVSRGVKPGDRV 60 Query: 61 AAQTEKSVSALVLYLATVRAGGVYLPLNTAYTLNELEYFITDAEPALVVCDPSKQDGIKA 120 AAQTEKSVS LVLYLATVRAGGVYLPLNTAYTLNEL+YFI DAEP +VVCDPSK +GI A Sbjct: 61 AAQTEKSVSGLVLYLATVRAGGVYLPLNTAYTLNELDYFIGDAEPTVVVCDPSKAEGIGA 120 Query: 121 IAAKVGAKVETLDASGKGSLTEAADKAAKEFATVPRGNNDLAAILYTSGTTGRSKGAMLS 180 +AAKVGAKVETLDASG+GSLT+AADKA F TVPR +DLAAILYTSGTTGRSKGAMLS Sbjct: 121 LAAKVGAKVETLDASGRGSLTDAADKAETAFITVPRAPDDLAAILYTSGTTGRSKGAMLS 180 Query: 181 HDNLASNSYSLVDYWRFSDKDVLIHALPIYHTHGLFVASNVTLFARASMIFLPKFDPDVI 240 HDNLASNS +L+DYWRF+ DVLIHALPIYHTHGLFVASNVTLFARASMIFLPK DPD+I Sbjct: 181 HDNLASNSLTLIDYWRFTRDDVLIHALPIYHTHGLFVASNVTLFARASMIFLPKLDPDLI 240 Query: 241 IKLMARATVMMGVPTFYTRLLQNPGLNKDATGHMRLFISGSAPLLADTHREWSARTGHAV 300 I LMARATV+MGVPTFYTRLLQNP L+K+ T HMRLFISGSAPLLADTHREW ARTGHAV Sbjct: 241 INLMARATVLMGVPTFYTRLLQNPRLSKETTSHMRLFISGSAPLLADTHREWFARTGHAV 300 Query: 301 LERYGMTETNMNTSNPYDGDRVPGAVGFALPGVSVRVTDPESGKELPRETIGMIEVKGPN 360 LERYGMTETNMNTSNPY G+RVPGAVGF LPGVSVRVTDPE+GKEL R+ IGMIEVKGPN Sbjct: 301 LERYGMTETNMNTSNPYAGERVPGAVGFPLPGVSVRVTDPETGKELARDDIGMIEVKGPN 360 Query: 361 VFQGYWRMPEKTKAEFRGDGFFITGDLGKIDDNGYVHILGRGKDLVISGGFNVYPKEIES 420 VF+GYWRMPEKTK+EFR DGFFITGDLGKID GYVHI+GRGKDLVISGGFNVYPKEIES Sbjct: 361 VFKGYWRMPEKTKSEFRPDGFFITGDLGKIDPQGYVHIVGRGKDLVISGGFNVYPKEIES 420 Query: 421 EIDAMPGVIESAVIGVPHADFGEGVTAVLVCSKGADVSEASVLKALDGRLAKFKMPKRVF 480 EIDAMPGV+ESAVIGVPHADFGEGVTAV+V GADVSE VLK LDGRLAKFKMPKRVF Sbjct: 421 EIDAMPGVVESAVIGVPHADFGEGVTAVVVRQPGADVSEVGVLKGLDGRLAKFKMPKRVF 480 Query: 481 VVEELPRNAMGKVQKNILRDTYKNIYAK 508 VV+ELPRN MGKVQKN+LRD YK+IY K Sbjct: 481 VVDELPRNTMGKVQKNVLRDQYKDIYTK 508 >ref|YP_578788.1| malonyl-CoA synthase [Nitrobacter hamburgensis X14] gb|ABE64328.1| AMP-dependent synthetase and ligase [Nitrobacter hamburgensis X14] Length = 505 Score = 872 bits (2252), Expect = 0.0, Method: Compositional matrix adjust. Identities = 424/503 (84%), Positives = 456/503 (90%) Query: 6 NANLFSRLFDGLDDPGRLAIEQLDGSRISYGDLIARAGQMANVLVERGVKPGDRVAAQTE 65 NANLFSRLFD LDD GR+AIE LDG RI+YGDLIA +G++AN LV RGVKPGDRVA Q E Sbjct: 2 NANLFSRLFDDLDDAGRIAIETLDGRRITYGDLIAFSGRLANTLVTRGVKPGDRVAVQVE 61 Query: 66 KSVSALVLYLATVRAGGVYLPLNTAYTLNELEYFITDAEPALVVCDPSKQDGIKAIAAKV 125 KSV L LYLATVRAG VYLPLNTAYTLNEL+YFITDAEPALVVCDPSK DGIKAIAAKV Sbjct: 62 KSVPNLALYLATVRAGAVYLPLNTAYTLNELDYFITDAEPALVVCDPSKADGIKAIAAKV 121 Query: 126 GAKVETLDASGKGSLTEAADKAAKEFATVPRGNNDLAAILYTSGTTGRSKGAMLSHDNLA 185 GAKVETL A GKGSLTEAA A FATV R ++DLAAILYTSGTTGRSKGAML+HDNLA Sbjct: 122 GAKVETLGAGGKGSLTEAAMTAKPAFATVARADDDLAAILYTSGTTGRSKGAMLTHDNLA 181 Query: 186 SNSYSLVDYWRFSDKDVLIHALPIYHTHGLFVASNVTLFARASMIFLPKFDPDVIIKLMA 245 SNS SLVD WRF+D D+LIHALPIYHTHGLFVA NVTLFARASMIFLPKFDPD++I+LMA Sbjct: 182 SNSCSLVDCWRFTDTDMLIHALPIYHTHGLFVAINVTLFARASMIFLPKFDPDLVIRLMA 241 Query: 246 RATVMMGVPTFYTRLLQNPGLNKDATGHMRLFISGSAPLLADTHREWSARTGHAVLERYG 305 RATV+MGVPTFYTRLLQNP L K++T +MRLFISGSAPLLADTHREWSARTGHAVLERYG Sbjct: 242 RATVLMGVPTFYTRLLQNPALTKESTRYMRLFISGSAPLLADTHREWSARTGHAVLERYG 301 Query: 306 MTETNMNTSNPYDGDRVPGAVGFALPGVSVRVTDPESGKELPRETIGMIEVKGPNVFQGY 365 MTET MNTSNPYDGDRVPGAVG AL GVSVRVTDPE+GKELPRETIGMIEVKGPNVF+GY Sbjct: 302 MTETGMNTSNPYDGDRVPGAVGRALRGVSVRVTDPETGKELPRETIGMIEVKGPNVFKGY 361 Query: 366 WRMPEKTKAEFRGDGFFITGDLGKIDDNGYVHILGRGKDLVISGGFNVYPKEIESEIDAM 425 WRMPEKT+AEFR DGFFITGD+GKID +VHILGRGKDLVISGGFNVYPKEIE EIDA+ Sbjct: 362 WRMPEKTRAEFRSDGFFITGDIGKIDGEDHVHILGRGKDLVISGGFNVYPKEIEGEIDAL 421 Query: 426 PGVIESAVIGVPHADFGEGVTAVLVCSKGADVSEASVLKALDGRLAKFKMPKRVFVVEEL 485 PGV+E+AVIGVPHADFGEGVTAV+VC+K AD+SEA VL ALDGRLAKFKMPKRV V+ L Sbjct: 422 PGVVETAVIGVPHADFGEGVTAVVVCNKDADISEAKVLGALDGRLAKFKMPKRVIFVDAL 481 Query: 486 PRNAMGKVQKNILRDTYKNIYAK 508 PRNAMGKVQKNILRDTY +Y + Sbjct: 482 PRNAMGKVQKNILRDTYAGLYRR 504 >ref|ZP_08627984.1| long-chain-fatty-acid--CoA ligase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP09279.1| long-chain-fatty-acid--CoA ligase [Bradyrhizobiaceae bacterium SG-6C] Length = 508 Score = 854 bits (2206), Expect = 0.0, Method: Compositional matrix adjust. Identities = 416/508 (81%), Positives = 462/508 (90%) Query: 1 MNTTTNANLFSRLFDGLDDPGRLAIEQLDGSRISYGDLIARAGQMANVLVERGVKPGDRV 60 MNT NANLFSRLFDGL + RLAIE+ DG+RISYGDLIA++G++ANVLV RGVKPGDRV Sbjct: 1 MNTAANANLFSRLFDGLTETSRLAIEKADGTRISYGDLIAQSGRVANVLVARGVKPGDRV 60 Query: 61 AAQTEKSVSALVLYLATVRAGGVYLPLNTAYTLNELEYFITDAEPALVVCDPSKQDGIKA 120 AAQTEKSV ALVLYLATVRAG VYLPLNTAYTLNEL+YFI DAEPAL+VCDPSK DGI Sbjct: 61 AAQTEKSVEALVLYLATVRAGAVYLPLNTAYTLNELDYFIGDAEPALIVCDPSKADGIGK 120 Query: 121 IAAKVGAKVETLDASGKGSLTEAADKAAKEFATVPRGNNDLAAILYTSGTTGRSKGAMLS 180 +AAK GAKVETLD GKGSLT+AA KA+ +FATVPR +DLAAILYTSGTTGRSKGAML+ Sbjct: 121 LAAKSGAKVETLDTEGKGSLTDAAAKASADFATVPRAGDDLAAILYTSGTTGRSKGAMLT 180 Query: 181 HDNLASNSYSLVDYWRFSDKDVLIHALPIYHTHGLFVASNVTLFARASMIFLPKFDPDVI 240 HDNLASNS SLV+YWRF+ DVLIHALPIYHTHGLFVASNVTLF+RA+MIFLPK DP+++ Sbjct: 181 HDNLASNSLSLVEYWRFTKDDVLIHALPIYHTHGLFVASNVTLFSRAAMIFLPKLDPELM 240 Query: 241 IKLMARATVMMGVPTFYTRLLQNPGLNKDATGHMRLFISGSAPLLADTHREWSARTGHAV 300 IKLM+RATVMMGVPTFYTRLLQ+P L+K+ T HMRLF+SGSAPLLA+THR+WSARTGHAV Sbjct: 241 IKLMSRATVMMGVPTFYTRLLQSPNLSKEPTKHMRLFVSGSAPLLAETHRDWSARTGHAV 300 Query: 301 LERYGMTETNMNTSNPYDGDRVPGAVGFALPGVSVRVTDPESGKELPRETIGMIEVKGPN 360 LERYGMTETNMNTSNPYDGDRVPGAVGF LPGV VRVTDPE+G EL R+ IGMIEVKGPN Sbjct: 301 LERYGMTETNMNTSNPYDGDRVPGAVGFPLPGVEVRVTDPETGDELKRDEIGMIEVKGPN 360 Query: 361 VFQGYWRMPEKTKAEFRGDGFFITGDLGKIDDNGYVHILGRGKDLVISGGFNVYPKEIES 420 VF+GYWRMPEKT +EFR DGFFITGDLGKID GYVHI+GRGKDLVISGGFNVYPKE+E+ Sbjct: 361 VFKGYWRMPEKTASEFRKDGFFITGDLGKIDARGYVHIIGRGKDLVISGGFNVYPKEVEN 420 Query: 421 EIDAMPGVIESAVIGVPHADFGEGVTAVLVCSKGADVSEASVLKALDGRLAKFKMPKRVF 480 EIDA+PGVIESAVIGVPHADFGEGVTAV+V K + EA+VL+ALDGRLAKFKMPKRV Sbjct: 421 EIDAIPGVIESAVIGVPHADFGEGVTAVVVGDKKTPLDEAAVLRALDGRLAKFKMPKRVL 480 Query: 481 VVEELPRNAMGKVQKNILRDTYKNIYAK 508 V++LPRN MGKVQKN+LRD+YK+IY K Sbjct: 481 FVDDLPRNTMGKVQKNVLRDSYKDIYGK 508