BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0023.p (286 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_770460.1| aldehyde dehydrogenase [Bradyrhizobium japonicu... 463 e-128 ref|YP_001204204.1| putative isoquinoline 1-oxidoreductase [Brad... 441 e-122 ref|YP_001238496.1| putative isoquinoline 1-oxidoreductase [Brad... 436 e-120 ref|ZP_08628992.1| isoquinoline 1-oxidoreductase beta subunit [B... 417 e-115 ref|YP_554640.1| isoquinoline 1-oxidoreductase [Burkholderia xen... 352 4e-95 >ref|NP_770460.1| aldehyde dehydrogenase [Bradyrhizobium japonicum USDA 110] dbj|BAC49085.1| bll3820 [Bradyrhizobium japonicum USDA 110] Length = 1174 Score = 463 bits (1191), Expect = e-128, Method: Compositional matrix adjust. Identities = 221/281 (78%), Positives = 246/281 (87%), Gaps = 1/281 (0%) Query: 1 MAQAPVRAETPANALAFPFNLRPLLAGWNALFHQAQAFQPDPSKSTIWNRGAYLIEGLGH 60 MAQ RA PAN LAFP N+RPLLAGWNALFHQ + F+PDP++S WNRGAYL+E LGH Sbjct: 891 MAQPAARATAPANTLAFPLNIRPLLAGWNALFHQTKDFKPDPAQSAQWNRGAYLVESLGH 950 Query: 61 CSACHSPRNTLGAERQAAYLAGGFAEGWEAPALTSLSKAPIPWSEDELYAYLRTGESRLH 120 CSACHSPRN LGAE++ AYLAGGFAEGWEAP LTSLS+APIPWSEDELYAYLRTG+SR H Sbjct: 951 CSACHSPRNALGAEQRDAYLAGGFAEGWEAPPLTSLSQAPIPWSEDELYAYLRTGQSRYH 1010 Query: 121 GVAAGPMAPVVQELAALPDQDIRAMAIYLASFNQTADRPAHDALAAKLEAGTVVTTAASA 180 GVAAGPMAPVV++L ALPDQDIRAMA+YL SFN A A +ALAA+LE+ T VTTAAS Sbjct: 1011 GVAAGPMAPVVRDLTALPDQDIRAMAVYLNSFNNAATE-APEALAARLESATQVTTAAST 1069 Query: 181 GARIYQGACAVCHEVGGLPLFGSRPSLALNSNLHSDAPDNLIQVILHGIAKPAVTDLGYM 240 GAR+YQGACAVCHEVGGLPLFGSRPSLALNSNLHS +PDNL+QVILHGIA+PA +DLGYM Sbjct: 1070 GARLYQGACAVCHEVGGLPLFGSRPSLALNSNLHSASPDNLVQVILHGIAEPASSDLGYM 1129 Query: 241 PAFKDSMSDGQIAELVTFLRRQFAPDKPAWTDVAATVGRIR 281 PAF++SMSD QI ELVTFLRRQFAPDKPAWT V T+ R+R Sbjct: 1130 PAFRNSMSDAQIEELVTFLRRQFAPDKPAWTGVRETIARVR 1170 >ref|YP_001204204.1| putative isoquinoline 1-oxidoreductase [Bradyrhizobium sp. ORS 278] emb|CAL75967.1| putative Isoquinoline 1-oxidoreductase [Bradyrhizobium sp. ORS 278] Length = 1179 Score = 441 bits (1134), Expect = e-122, Method: Compositional matrix adjust. Identities = 209/285 (73%), Positives = 240/285 (84%), Gaps = 1/285 (0%) Query: 1 MAQAPVRAETPANALAFPFNLRPLLAGWNALFHQAQAFQPDPSKSTIWNRGAYLIEGLGH 60 MAQ PVR ++P N L FPFNLRPL+AGWNALFH + F+PDP++S WNRG YLIE LGH Sbjct: 893 MAQQPVRQQSPDNQLRFPFNLRPLVAGWNALFHSTETFKPDPTQSEQWNRGFYLIESLGH 952 Query: 61 CSACHSPRNTLGAERQAAYLAGGFAEGWEAPALTSLSKAPIPWSEDELYAYLRTGESRLH 120 CSACHSPRN LGAE++ AYLAGGFA+GWEAPALT+LS APIPW++DEL+ YLR+G+SR H Sbjct: 953 CSACHSPRNALGAEQRNAYLAGGFADGWEAPALTALSSAPIPWTDDELFTYLRSGQSRFH 1012 Query: 121 GVAAGPMAPVVQELAALPDQDIRAMAIYLASFNQTADRPAH-DALAAKLEAGTVVTTAAS 179 GVAAGPMAP+V++LA LPD DIRAMA YLASF + PA +A+A+ LEA T VT+A+S Sbjct: 1013 GVAAGPMAPIVKDLAKLPDADIRAMAAYLASFQTPSPEPAKLEAIASDLEARTKVTSASS 1072 Query: 180 AGARIYQGACAVCHEVGGLPLFGSRPSLALNSNLHSDAPDNLIQVILHGIAKPAVTDLGY 239 AR+Y GACAVCHEVGGLPLFGSRPSLALNSNLHS PDNLIQVILHGI KPA +DLGY Sbjct: 1073 PAARLYLGACAVCHEVGGLPLFGSRPSLALNSNLHSATPDNLIQVILHGIMKPASSDLGY 1132 Query: 240 MPAFKDSMSDGQIAELVTFLRRQFAPDKPAWTDVAATVGRIRQTI 284 MPAFKD +SD Q+AELV++LR QFAPDKPAWTDV V RIR TI Sbjct: 1133 MPAFKDHLSDAQLAELVSYLRGQFAPDKPAWTDVEDAVSRIRSTI 1177 >ref|YP_001238496.1| putative isoquinoline 1-oxidoreductase [Bradyrhizobium sp. BTAi1] gb|ABQ34590.1| putative Isoquinoline 1-oxidoreductase [Bradyrhizobium sp. BTAi1] Length = 1181 Score = 436 bits (1120), Expect = e-120, Method: Compositional matrix adjust. Identities = 209/282 (74%), Positives = 237/282 (84%), Gaps = 1/282 (0%) Query: 1 MAQAPVRAETPANALAFPFNLRPLLAGWNALFHQAQAFQPDPSKSTIWNRGAYLIEGLGH 60 MAQ PVR + P N L FPFNLRPL+AGWNALFH Q F+ DP+KS WNRG YL+E LGH Sbjct: 895 MAQPPVRQQAPDNQLRFPFNLRPLVAGWNALFHDNQPFKSDPTKSEQWNRGFYLVESLGH 954 Query: 61 CSACHSPRNTLGAERQAAYLAGGFAEGWEAPALTSLSKAPIPWSEDELYAYLRTGESRLH 120 CSACHSPRN LGAE++ AYLAGGFA+GWEAPALTSLS APIPW+EDELY YLR+G+SR H Sbjct: 955 CSACHSPRNALGAEQRKAYLAGGFADGWEAPALTSLSSAPIPWTEDELYTYLRSGQSRFH 1014 Query: 121 GVAAGPMAPVVQELAALPDQDIRAMAIYLASFNQTADRPAH-DALAAKLEAGTVVTTAAS 179 GVAAGPMAP+V++LA L D DIRAMA YLASF + A PA +A+AA+LEA T VT+A+S Sbjct: 1015 GVAAGPMAPIVKDLATLADADIRAMATYLASFQEAAPEPATLEAMAAELEALTQVTSASS 1074 Query: 180 AGARIYQGACAVCHEVGGLPLFGSRPSLALNSNLHSDAPDNLIQVILHGIAKPAVTDLGY 239 AR+Y GACAVCHEVGGLPLFGSRPSLALNSNLHS PDNLIQVILHGI KPA +DLGY Sbjct: 1075 PAARLYLGACAVCHEVGGLPLFGSRPSLALNSNLHSATPDNLIQVILHGIMKPASSDLGY 1134 Query: 240 MPAFKDSMSDGQIAELVTFLRRQFAPDKPAWTDVAATVGRIR 281 MPAF+D +SD Q+AELV++LR QFAPDKP WT+V A V RIR Sbjct: 1135 MPAFRDHLSDTQLAELVSYLRGQFAPDKPGWTNVEAAVSRIR 1176 >ref|ZP_08628992.1| isoquinoline 1-oxidoreductase beta subunit [Bradyrhizobiaceae bacterium SG-6C] gb|EGP08351.1| isoquinoline 1-oxidoreductase beta subunit [Bradyrhizobiaceae bacterium SG-6C] Length = 1188 Score = 417 bits (1073), Expect = e-115, Method: Compositional matrix adjust. Identities = 205/288 (71%), Positives = 237/288 (82%), Gaps = 5/288 (1%) Query: 1 MAQAPVRAETPANALAFPFNLRPLLAGWNALFHQAQAFQPDPSKSTIWNRGAYLIEGLGH 60 +AQ VRA ALAFPFNLRPL+AGWNALFH+ F+ D ++S WNRGAYL+E LGH Sbjct: 898 IAQPAVRATNRETALAFPFNLRPLMAGWNALFHKPAVFRADAAQSETWNRGAYLVESLGH 957 Query: 61 CSACHSPRNTLGAERQA-AYLAGGFAEGWEAPALTSLSKAPIPWSEDELYAYLRTGESRL 119 C ACH+PRN LGAER A AYLAGG A+GWEAP LTSLS+APIPW+EDEL+AYLRTG SR Sbjct: 958 CGACHTPRNALGAERDARAYLAGGVADGWEAPPLTSLSQAPIPWTEDELFAYLRTGASRF 1017 Query: 120 HGVAAGPMAPVVQELAALPDQDIRAMAIYLASFNQTADRPA-HDALAAKLEAGTVVTTAA 178 HGVAAGPMAPVV+ELAA+PD DI AMA+YLASFN+T PA DALAA+LE T Sbjct: 1018 HGVAAGPMAPVVKELAAVPDSDIHAMAVYLASFNETKITPAAQDALAARLEDATRARRTP 1077 Query: 179 SA---GARIYQGACAVCHEVGGLPLFGSRPSLALNSNLHSDAPDNLIQVILHGIAKPAVT 235 SA GARIY+GACAVCH+VGG LFG+RPSLALNSNLHS PDNLIQVILHGI +P + Sbjct: 1078 SASPVGARIYEGACAVCHQVGGPVLFGTRPSLALNSNLHSAMPDNLIQVILHGIEQPVSS 1137 Query: 236 DLGYMPAFKDSMSDGQIAELVTFLRRQFAPDKPAWTDVAATVGRIRQT 283 DLGYMPAF++S+SDGQ+AELV++LR+QFAPDK WTD+AATV RIR++ Sbjct: 1138 DLGYMPAFRNSLSDGQVAELVSYLRQQFAPDKAPWTDLAATVDRIRRS 1185 >ref|YP_554640.1| isoquinoline 1-oxidoreductase [Burkholderia xenovorans LB400] gb|ABE35290.1| Isoquinoline 1-oxidoreductase [Burkholderia xenovorans LB400] Length = 1202 Score = 352 bits (903), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 174/286 (60%), Positives = 211/286 (73%), Gaps = 3/286 (1%) Query: 1 MAQAPVRAETPANALAFPFNLRPLLAGWNALFHQAQAFQPDPSKSTIWNRGAYLIEGLGH 60 MAQ PVR+ P LA+PF++RPL+A WN LF AF ++S WNRGAYL+ GLGH Sbjct: 913 MAQTPVRSRAPETKLAYPFSVRPLMAAWNGLFLGRTAFAASATQSAQWNRGAYLVNGLGH 972 Query: 61 CSACHSPRNTLGAERQ-AAYLAGGFAEGWEAPALTSLSKAPIPWSEDELYAYLRTGESRL 119 CSACH+PRN GAE+ AA++ GG AEGWEAPAL++LS AP+PWSEDEL++YLR G + L Sbjct: 973 CSACHTPRNAFGAEKTGAAFMGGGIAEGWEAPALSTLSTAPVPWSEDELFSYLRHGHAPL 1032 Query: 120 HGVAAGPMAPVVQELAALPDQDIRAMAIYLASFNQTADRPAHDALAAKLEAGTVVT-TAA 178 HGVAAGPMAPVV +LAALPD DIRAMA YLAS NQ A A ++E + +T A Sbjct: 1033 HGVAAGPMAPVVGDLAALPDSDIRAMATYLASLNQLEPNANPAATAHQVEQASAITGMPA 1092 Query: 179 SAGARIYQGACAVCHEVGGLP-LFGSRPSLALNSNLHSDAPDNLIQVILHGIAKPAVTDL 237 GAR++ GACA CH G P LFG+ PSLALN+NLHS PDNLI+VIL GI PA +L Sbjct: 1093 GLGARLFDGACAACHHTGSGPQLFGAHPSLALNTNLHSATPDNLIRVILDGIGSPARPEL 1152 Query: 238 GYMPAFKDSMSDGQIAELVTFLRRQFAPDKPAWTDVAATVGRIRQT 283 G MPA++DS +D Q+AELV++LR+QFA KPAW DV A+V RIR T Sbjct: 1153 GTMPAYRDSFNDAQVAELVSYLRQQFAGGKPAWQDVTASVARIRAT 1198