BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0246.0003.p (406 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_002287747.1| secretion protein HlyD [Oligotropha carboxid... 429 e-118 ref|ZP_08629996.1| cobalt/zinc/cadmium efflux RND transporter me... 367 2e-99 ref|YP_002961235.1| efflux transporter, RND family, MFP subunit ... 360 3e-97 ref|YP_003066053.1| secretion protein , RND family efflux protei... 357 1e-96 ref|ZP_07025756.1| efflux transporter, RND family, MFP subunit [... 338 1e-90 >ref|YP_002287747.1| secretion protein HlyD [Oligotropha carboxidovorans OM5] ref|YP_004634127.1| cation efflux system protein CzcB [Oligotropha carboxidovorans OM5] gb|ACI91882.1| secretion protein HlyD [Oligotropha carboxidovorans OM5] gb|AEI04256.1| cation efflux system protein CzcB [Oligotropha carboxidovorans OM4] gb|AEI07886.1| cation efflux system protein CzcB [Oligotropha carboxidovorans OM5] Length = 403 Score = 429 bits (1102), Expect = e-118, Method: Compositional matrix adjust. Identities = 228/409 (55%), Positives = 288/409 (70%), Gaps = 12/409 (2%) Query: 1 MKRVVLFFAGVAAGIALVVAAAGWIGWPVRFPAATQETATTDTKTEARH------EAPGH 54 MKR + + A A GIA +AA W PAA + + E H E H Sbjct: 1 MKRSIFWAALAALGIAATIAAMNW------RPAAKTGGSHSHHAAEGAHGHGGPTEHADH 54 Query: 55 IELSEDQVREAGITFAQASGGMLKRHFLAPGSLIPDADHIGRVSVRVLATVTELRKRLGD 114 IEL++ Q+ AGIT A L HFLAPG+++PDAD IGRV+VRVL TVTELRKR+GD Sbjct: 55 IELTDAQIAAAGITLAPVEAKTLSEHFLAPGTIVPDADRIGRVAVRVLGTVTELRKRVGD 114 Query: 115 VVEKGEIVAAIESREVADAKSEYLAARLTNDLQQTLYARQKTLVETRIVSENEFLRTRLT 174 +VEK E+VA +ESRE+++AKSEYLAARLTNDLQQTL R K L E+R ENE+L++RLT Sbjct: 115 MVEKDEVVATLESRELSEAKSEYLAARLTNDLQQTLATRHKQLWESRSFPENEYLKSRLT 174 Query: 175 ANDAQIKLDGARQKLFALGLSESEIADLPNQPPESLRTQFLRAPISGRVSERRVDLGGLI 234 A DA+I+ D A QKL ALGL+++EIA LP+ P E LR Q LR+P+ GR++ERRVDLG L+ Sbjct: 175 AQDARIRFDSAHQKLLALGLTDTEIAQLPDLPIEELRKQSLRSPLRGRIAERRVDLGALV 234 Query: 235 GREGQESELYVIVNLDDIWVDLAVSPEDIGAVREGVSVKIRAIGTEDEASAGVIFVSPLL 294 GREGQESEL+VIV+LD +W+DLA+SP D+ V EG +V I A +A ++F+SPL+ Sbjct: 235 GREGQESELFVIVDLDKVWIDLAISPGDLSKVVEGSTVNITAGPDGPTGAAQIVFISPLI 294 Query: 295 DRETRNARVIATMPNKDHRWKPGTFVTAEVPLSGAPSTVIVSKKALQTIKGTPTVFVRDA 354 D++TR ARVIAT+ N W+PG FVTAEVP++ + V K ALQT+ G PT+FV+ Sbjct: 295 DKDTRAARVIATLDNAGAHWRPGMFVTAEVPVANRGPVLAVPKAALQTLDGAPTLFVKQD 354 Query: 355 DGFEARSVRTGREDDDDIEIVAGLAAGETIAVQNTFTLKAEFEKDEAEH 403 +GF R VR GREDD+ EIVAG+ AGE IA+ NTFTLKAE K EAEH Sbjct: 355 EGFAIRKVRLGREDDERQEIVAGVNAGEVIAISNTFTLKAELGKGEAEH 403 >ref|ZP_08629996.1| cobalt/zinc/cadmium efflux RND transporter membrane fusion protein CzcB [Bradyrhizobiaceae bacterium SG-6C] gb|EGP07365.1| cobalt/zinc/cadmium efflux RND transporter membrane fusion protein CzcB [Bradyrhizobiaceae bacterium SG-6C] Length = 393 Score = 367 bits (942), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 193/390 (49%), Positives = 254/390 (65%), Gaps = 22/390 (5%) Query: 32 PAATQETATTDTKTEARHEAP-----------GHIELSEDQVREAGITFAQASGGMLKRH 80 P A AT T HE P G I L+E Q+ ++A+ G L + Sbjct: 10 PGALCAIATLFFTTAGAHETPPTQKQSASHSDGGIRLTERQITAGNFAISEAAPGTLTKR 69 Query: 81 FLAPGSLIPDADHIGRVSVRVLATVTELRKRLGDVVEKGEIVAAIESREVADAKSEYLAA 140 PG+++P +HI +VSVR+L TV ELRKRLGD V +GE++A IESREVADAKS+YLAA Sbjct: 70 IAVPGTIVPSGEHIAKVSVRLLGTVAELRKRLGDTVREGEVLAIIESREVADAKSQYLAA 129 Query: 141 RLTNDLQQTLYARQKTLVETRIVSENEFLRTRLTANDAQIKLDGARQKLFALGLSESEIA 200 R+ DLQ+TL+ R K+L E+++ SEN++LR R D +IK D ARQKLFALGL+ +I Sbjct: 130 RIAFDLQETLFNRSKSLFESKVSSENDYLRARTAFEDIRIKHDVARQKLFALGLTNDQIV 189 Query: 201 DLPNQPPESLRTQFLRAPISGRVSERRVDLGGLIGREGQESELYVIVNLDDIWVDLAVSP 260 LP QP ESLR Q LRAP+SGR+++RRVDLG L+GREGQESEL+ IV+ +W +LA+S Sbjct: 190 ALPQQPVESLRLQELRAPMSGRIADRRVDLGSLVGREGQESELFTIVDSSTVWAELALSV 249 Query: 261 EDIGAVREGVSVKIRAIGTEDEASAG-----VIFVSPLLDRETRNARVIATMPNKDHRWK 315 D+ VREG+ + I D ASAG ++FVSPLLD++TR ARV+A++ N + Sbjct: 250 TDLAPVREGLQISI------DTASAGHLPATIMFVSPLLDKDTRLARVVASVDNPADMLR 303 Query: 316 PGTFVTAEVPLSGAPSTVIVSKKALQTIKGTPTVFVRDADGFEARSVRTGREDDDDIEIV 375 PG F+TA +P + VIV K A+Q+I G VFVR DGF R + TGRED E+ Sbjct: 304 PGMFITAHIPSQQVKADVIVPKAAVQSIDGRNAVFVRTPDGFMPRKIVTGREDGRSFEVT 363 Query: 376 AGLAAGETIAVQNTFTLKAEFEKDEAEHDH 405 +GL+ GE IA NTF LKA+ +DE +H H Sbjct: 364 SGLSPGEQIATANTFVLKADLGRDETQHAH 393 >ref|YP_002961235.1| efflux transporter, RND family, MFP subunit [Methylobacterium extorquens AM1] gb|ACS44128.1| efflux transporter, RND family, MFP subunit [Methylobacterium extorquens AM1] Length = 427 Score = 360 bits (923), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 195/426 (45%), Positives = 267/426 (62%), Gaps = 26/426 (6%) Query: 1 MKRVVLFFAGVAAGIALVVAAAGWIGWP--------------VRFPAA-TQETATTDTKT 45 M R++ AG G V+A W G P FPA ET D + Sbjct: 3 MVRIIFLVAGALVG-GFVIATV-WPGAPDTLRAALAGRSVAGFTFPAPRAGETTNADAQP 60 Query: 46 EARHEA---------PGHIELSEDQVREAGITFAQASGGMLKRHFLAPGSLIPDADHIGR 96 + + EA PG ++L++ Q+ +AGI A A GG L RH PG+++P A++ R Sbjct: 61 KKKVEAEPQPDHGDKPGLVKLTDAQIDKAGIRAAPAVGGRLTRHLHVPGTIVPSANNTAR 120 Query: 97 VSVRVLATVTELRKRLGDVVEKGEIVAAIESREVADAKSEYLAARLTNDLQQTLYARQKT 156 ++V+ V ELRK LGD V KGEIVA ++SR++AD K EYLAARL+ DLQ+TLY R K Sbjct: 121 IAVKTQGIVAELRKSLGDKVAKGEIVALLDSRDIADTKGEYLAARLSADLQKTLYERDKA 180 Query: 157 LVETRIVSENEFLRTRLTANDAQIKLDGARQKLFALGLSESEIADLPNQPPESLRTQFLR 216 L + RI SE ++LR++ + D ++K D AR+KL LGLS++EI LPNQP Q +R Sbjct: 181 LWDKRISSEQQYLRSQASYQDLKVKEDAARRKLTFLGLSQAEIDALPNQPEAQFERQEIR 240 Query: 217 APISGRVSERRVDLGGLIGREGQESELYVIVNLDDIWVDLAVSPEDIGAVREGVSVKIRA 276 +PI+G++ ERRVDLG +GR+ E+ELY +V+L +WVDLAVSP D+ VREG +V +R Sbjct: 241 SPIAGKIVERRVDLGAAVGRDNLETELYSVVDLSKVWVDLAVSPGDLPLVREGQTVTVRT 300 Query: 277 IGTEDEASAGVIFVSPLLDRETRNARVIATMPNKDHRWKPGTFVTAEVPLSGAPSTVIVS 336 T +E ++F+ P+LD+ TR ARV+A + N D W+PG+FV AE+ +S V+V Sbjct: 301 EVTGEEGRGRIVFIGPVLDQNTRAARVVAELANPDETWRPGSFVAAEIEVSDKAVRVLVP 360 Query: 337 KKALQTIKGTPTVFVRDADGFEARSVRTGREDDDDIEIVAGLAAGETIAVQNTFTLKAEF 396 A+QT++G P VFVR ADGFEAR V GR DD ++E+ AGL GE I V N+FTLK+E Sbjct: 361 ADAVQTLEGKPNVFVRVADGFEARPVTVGRSDDRNVEVTAGLEPGEVIVVANSFTLKSEL 420 Query: 397 EKDEAE 402 K AE Sbjct: 421 GKAAAE 426 >ref|YP_003066053.1| secretion protein , RND family efflux protein [Methylobacterium extorquens DM4] emb|CAX21994.1| putative secretion protein precursor, putative RND family efflux protein [Methylobacterium extorquens DM4] Length = 407 Score = 357 bits (917), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 191/411 (46%), Positives = 261/411 (63%), Gaps = 15/411 (3%) Query: 1 MKRVVLFFAGVAAGIALVVAAAGWIGWP---------VRFPAATQETATTDTKTEARHEA 51 +KR+++ V +G + A W WP + P A + K + E Sbjct: 2 LKRLLIIPLAVVSGFLI----ATW--WPAGPDALRALLTGPRHEHAIARPEVKPASIPER 55 Query: 52 PGHIELSEDQVREAGITFAQASGGMLKRHFLAPGSLIPDADHIGRVSVRVLATVTELRKR 111 G + LS +QV AGI + G L RH PG+++P AD R++V+ TV ELRK Sbjct: 56 SGLVRLSGEQVARAGIRVSPVGEGRLSRHLHVPGTIVPRADGTARIAVKTTGTVVELRKG 115 Query: 112 LGDVVEKGEIVAAIESREVADAKSEYLAARLTNDLQQTLYARQKTLVETRIVSENEFLRT 171 LGD V KGE+VA ++SRE+ADAK EYLAAR+T LQ+TLY R K L + R+ SE ++LR+ Sbjct: 116 LGDKVAKGEVVALLDSREIADAKGEYLAARVTAALQKTLYERDKELWDKRVSSEQQYLRS 175 Query: 172 RLTANDAQIKLDGARQKLFALGLSESEIADLPNQPPESLRTQFLRAPISGRVSERRVDLG 231 + + D ++K + AR+KL LGLS++EI LPNQP Q +R+P+SG+V ERRVDLG Sbjct: 176 QASYQDLKVKEEAARRKLTFLGLSQAEIQALPNQPEAQFERQEIRSPLSGKVVERRVDLG 235 Query: 232 GLIGREGQESELYVIVNLDDIWVDLAVSPEDIGAVREGVSVKIRAIGTEDEASAGVIFVS 291 +GR+ E+ELY +V+L +W+DLAVSP D+ VREG +V +R T A ++F+ Sbjct: 236 AAVGRDNLETELYSVVDLSTVWIDLAVSPSDLPLVREGQTVVVRVDATGQAARGRIVFIV 295 Query: 292 PLLDRETRNARVIATMPNKDHRWKPGTFVTAEVPLSGAPSTVIVSKKALQTIKGTPTVFV 351 P+LD+ TR ARV+A + N D W+PG+FV AE+ +S P V++ A+QT++G PTVFV Sbjct: 296 PVLDQATRAARVVAELANPDEIWRPGSFVAAEIAISDKPVPVLIPATAIQTVEGKPTVFV 355 Query: 352 RDADGFEARSVRTGREDDDDIEIVAGLAAGETIAVQNTFTLKAEFEKDEAE 402 R ADGFE R VR GR DDD +E+ AGL GE IAV NTFTLK+E K AE Sbjct: 356 RVADGFETRPVRLGRSDDDGVEVTAGLEPGEFIAVANTFTLKSELGKVAAE 406 >ref|ZP_07025756.1| efflux transporter, RND family, MFP subunit [Afipia sp. 1NLS2] gb|EFI52898.1| efflux transporter, RND family, MFP subunit [Afipia sp. 1NLS2] Length = 404 Score = 338 bits (866), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 172/355 (48%), Positives = 240/355 (67%) Query: 44 KTEARHEAPGHIELSEDQVREAGITFAQASGGMLKRHFLAPGSLIPDADHIGRVSVRVLA 103 K A G + +S++Q++ A I + + R + PGS++PDA+ I VSV++ Sbjct: 45 KPSAPEPISGLLSMSDEQIKLAQIDLVEVGPAAIARRLVVPGSVVPDANLIAHVSVKLSG 104 Query: 104 TVTELRKRLGDVVEKGEIVAAIESREVADAKSEYLAARLTNDLQQTLYARQKTLVETRIV 163 TV ELRK +G+ V K E++A +ESREVADAKSEYLAA+LTNDLQQ L R KTL ++R+ Sbjct: 105 TVAELRKNIGEDVAKDEVIAVLESREVADAKSEYLAAKLTNDLQQDLSTRDKTLWDSRVG 164 Query: 164 SENEFLRTRLTANDAQIKLDGARQKLFALGLSESEIADLPNQPPESLRTQFLRAPISGRV 223 +E ++LR+R A ++++ ARQKL ALGL E EI +P P SLR Q +R+PISGRV Sbjct: 165 TEQQYLRSRNAAAQTEMRVKIARQKLLALGLGEKEITAVPGAPEASLRRQDVRSPISGRV 224 Query: 224 SERRVDLGGLIGREGQESELYVIVNLDDIWVDLAVSPEDIGAVREGVSVKIRAIGTEDEA 283 +ER+V+LG +GR+ E+EL+V+V+L +WV+L+V+ D+ ++EG SVKI A G + Sbjct: 225 AERKVELGTAVGRDSLETELFVVVDLSRVWVELSVASSDLQLIKEGQSVKISARGLAETT 284 Query: 284 SAGVIFVSPLLDRETRNARVIATMPNKDHRWKPGTFVTAEVPLSGAPSTVIVSKKALQTI 343 +IFVSPL+D++TR ARV+A + N D W+PG+FVTA + L+ V V A+Q + Sbjct: 285 IGKIIFVSPLVDKDTRTARVVAEISNPDQTWRPGSFVTAGIMLNERTVAVAVPATAIQKL 344 Query: 344 KGTPTVFVRDADGFEARSVRTGREDDDDIEIVAGLAAGETIAVQNTFTLKAEFEK 398 G P VFVR DGFE R+V G +D IEIV+G+ GETIA NTF+LKAE K Sbjct: 345 DGQPIVFVRTKDGFEKRNVVVGESEDQAIEIVSGVTPGETIATTNTFSLKAELSK 399