BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0243.0055.p (319 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_769521.1| epoxide hydrolase [Bradyrhizobium japonicum USD... 563 e-158 ref|YP_001204572.1| putative epoxide hydrolase [Bradyrhizobium s... 559 e-157 ref|YP_001238893.1| putative epoxide hydrolase [Bradyrhizobium s... 552 e-155 ref|YP_531536.1| alpha/beta hydrolase fold protein [Rhodopseudom... 541 e-152 gb|ACO95125.1| epoxide hydrolase [uncultured organism] 536 e-150 >ref|NP_769521.1| epoxide hydrolase [Bradyrhizobium japonicum USDA 110] dbj|BAC48146.1| epoxide hydrolase [Bradyrhizobium japonicum USDA 110] Length = 348 Score = 563 bits (1451), Expect = e-158, Method: Compositional matrix adjust. Identities = 274/331 (82%), Positives = 295/331 (89%), Gaps = 12/331 (3%) Query: 1 MIEMPPLQFAETNGIRMGFYEAGPKTDTPPVMLCHGWPELAFSWRHQIKALGEAGIRVIA 60 MIEMPPL+F +TNGIRMG+YEAGP +DTPP++LCHGWPELAFSWRHQIKAL EAGIRVIA Sbjct: 18 MIEMPPLKFVQTNGIRMGYYEAGPVSDTPPMVLCHGWPELAFSWRHQIKALSEAGIRVIA 77 Query: 61 PDQRGYGATDRPEPVEAYDMEHLTGDLVGLLDHLKIDKAIFVGHDWGGFVVWQMPLRHPA 120 PDQRGYGATDRPEPVE YD+EHLTGDLVGLLDHL IDKAIFVGHDWGGF+VWQMPLRH Sbjct: 78 PDQRGYGATDRPEPVEDYDIEHLTGDLVGLLDHLGIDKAIFVGHDWGGFIVWQMPLRHIG 137 Query: 121 RVAGVVGVNTPHWDRAPMDPIALFRQRFGDHMYIVQFQDPGREPDRIFGSRVEQTFDAFM 180 RVAGVVG+NTPH +RA DPI L R RFGD MYIVQFQDP RE DRIFGSRVEQTFDAFM Sbjct: 138 RVAGVVGINTPHTNRAWADPIELLRARFGDKMYIVQFQDPSREADRIFGSRVEQTFDAFM 197 Query: 181 RKPA------------AGVGASARLNLAFPQMIANYDARHDPRTPILSADEKKVFVDTFT 228 RKPA AGVGAS R+NLAFPQMIA YDA+HDPRTPILS +EKKVFVD FT Sbjct: 198 RKPAPRPPDAPAEEVIAGVGASPRVNLAFPQMIAAYDAKHDPRTPILSPEEKKVFVDNFT 257 Query: 229 KTGFTGGINWYRNFTRNWERSAGLDHHVRVPSLMIMAENDAVLPPSAADGMERLVPDLEK 288 +TGFTGGINWYRN +RNW RS GLDH VRVPSLMIMAENDAVLPPS+ADGM++L+PDLEK Sbjct: 258 RTGFTGGINWYRNMSRNWIRSEGLDHTVRVPSLMIMAENDAVLPPSSADGMDKLIPDLEK 317 Query: 289 YLVKDSGHWTQQEKPDEVSAKLIEWRRKRFG 319 YLV+DSGHWTQQEKPDEVSAKLIEWRR+RFG Sbjct: 318 YLVRDSGHWTQQEKPDEVSAKLIEWRRRRFG 348 >ref|YP_001204572.1| putative epoxide hydrolase [Bradyrhizobium sp. ORS 278] emb|CAL76335.1| putative epoxide hydrolase [Bradyrhizobium sp. ORS 278] Length = 334 Score = 559 bits (1440), Expect = e-157, Method: Compositional matrix adjust. Identities = 268/329 (81%), Positives = 290/329 (88%), Gaps = 12/329 (3%) Query: 3 EMPPLQFAETNGIRMGFYEAGPKTDTPPVMLCHGWPELAFSWRHQIKALGEAGIRVIAPD 62 MPPLQ+A TNGIRMG+YEAGP TD PP++LCHGWPELAFSWRHQIKAL EAGIRVIAPD Sbjct: 6 SMPPLQYATTNGIRMGYYEAGPATDHPPMILCHGWPELAFSWRHQIKALAEAGIRVIAPD 65 Query: 63 QRGYGATDRPEPVEAYDMEHLTGDLVGLLDHLKIDKAIFVGHDWGGFVVWQMPLRHPARV 122 QRGYGATDRPEPVE+YD+EHLT DLVGLLDHL+IDKAIFVGHDWGGFVVWQMPLRHP RV Sbjct: 66 QRGYGATDRPEPVESYDLEHLTADLVGLLDHLEIDKAIFVGHDWGGFVVWQMPLRHPDRV 125 Query: 123 AGVVGVNTPHWDRAPMDPIALFRQRFGDHMYIVQFQDPGREPDRIFGSRVEQTFDAFMRK 182 AGVVG+NTPH RAP DPIA+ R+RFG+ MYIVQFQDP REPDRIFGSRVEQTFDAFMRK Sbjct: 126 AGVVGINTPHLPRAPADPIAIMRKRFGEMMYIVQFQDPAREPDRIFGSRVEQTFDAFMRK 185 Query: 183 P------------AAGVGASARLNLAFPQMIANYDARHDPRTPILSADEKKVFVDTFTKT 230 P A G+ AS LNLAFPQ IA YDA D R PILSA EK+VFV+TF++T Sbjct: 186 PLPRGDAPSTEPQAGGIAASPTLNLAFPQTIAAYDASKDTRQPILSAAEKRVFVETFSRT 245 Query: 231 GFTGGINWYRNFTRNWERSAGLDHHVRVPSLMIMAENDAVLPPSAADGMERLVPDLEKYL 290 GFTGGINWYRN TRNW+RS GLDH VRVPSLMIMAENDAVLPPSAADGME+LVPDLEKYL Sbjct: 246 GFTGGINWYRNMTRNWQRSEGLDHTVRVPSLMIMAENDAVLPPSAADGMEKLVPDLEKYL 305 Query: 291 VKDSGHWTQQEKPDEVSAKLIEWRRKRFG 319 V+DSGHWTQQE+P+EVSAKLIEWRR+RFG Sbjct: 306 VRDSGHWTQQEQPEEVSAKLIEWRRRRFG 334 >ref|YP_001238893.1| putative epoxide hydrolase [Bradyrhizobium sp. BTAi1] gb|ABQ34987.1| putative epoxide hydrolase [Bradyrhizobium sp. BTAi1] Length = 334 Score = 552 bits (1422), Expect = e-155, Method: Compositional matrix adjust. Identities = 267/328 (81%), Positives = 285/328 (86%), Gaps = 12/328 (3%) Query: 4 MPPLQFAETNGIRMGFYEAGPKTDTPPVMLCHGWPELAFSWRHQIKALGEAGIRVIAPDQ 63 MPPLQFA TNGIRMG+YEAGP +D PP++LCHGWPELAFSWRHQI+AL EAGIRVIAPDQ Sbjct: 7 MPPLQFATTNGIRMGYYEAGPASDRPPLILCHGWPELAFSWRHQIRALAEAGIRVIAPDQ 66 Query: 64 RGYGATDRPEPVEAYDMEHLTGDLVGLLDHLKIDKAIFVGHDWGGFVVWQMPLRHPARVA 123 RGYGATDRPE VEAYD+E LT DLVGLLDHLKIDKA+FVGHDWGGFVVWQMPLRHP RVA Sbjct: 67 RGYGATDRPEAVEAYDLEQLTADLVGLLDHLKIDKAVFVGHDWGGFVVWQMPLRHPDRVA 126 Query: 124 GVVGVNTPHWDRAPMDPIALFRQRFGDHMYIVQFQDPGREPDRIFGSRVEQTFDAFMRK- 182 GVVG+NTPH RAP DPIA+ R+RFGD MYIVQFQDP REPDRIF +RVEQTFDAFMRK Sbjct: 127 GVVGINTPHLPRAPADPIAIMRKRFGDMMYIVQFQDPAREPDRIFANRVEQTFDAFMRKP 186 Query: 183 -----------PAAGVGASARLNLAFPQMIANYDARHDPRTPILSADEKKVFVDTFTKTG 231 PA G+ A LNLAFPQMIA YDA D R PILS EK+VFV+TFT+TG Sbjct: 187 LPRHDAPPTEPPAGGIAAPPSLNLAFPQMIAAYDAAKDTRQPILSEAEKRVFVETFTRTG 246 Query: 232 FTGGINWYRNFTRNWERSAGLDHHVRVPSLMIMAENDAVLPPSAADGMERLVPDLEKYLV 291 FTGGINWYRN TRNW+RS GLDH VRVPSLMIMAENDAVLPPSAADGMERLVPDLEKYLV Sbjct: 247 FTGGINWYRNMTRNWQRSEGLDHTVRVPSLMIMAENDAVLPPSAADGMERLVPDLEKYLV 306 Query: 292 KDSGHWTQQEKPDEVSAKLIEWRRKRFG 319 KDSGHWTQQE+P EVSAKLIEWRR+RFG Sbjct: 307 KDSGHWTQQEQPAEVSAKLIEWRRRRFG 334 >ref|YP_531536.1| alpha/beta hydrolase fold protein [Rhodopseudomonas palustris BisB18] gb|ABD87217.1| alpha/beta hydrolase fold [Rhodopseudomonas palustris BisB18] Length = 331 Score = 541 bits (1395), Expect = e-152, Method: Compositional matrix adjust. Identities = 262/331 (79%), Positives = 286/331 (86%), Gaps = 12/331 (3%) Query: 1 MIEMPPLQFAETNGIRMGFYEAGPKTDTPPVMLCHGWPELAFSWRHQIKALGEAGIRVIA 60 MIEMPPLQFA +NGIR+G+Y+AGP TDTPP++LCHGWPELAFSWRHQIKAL EAGIRVIA Sbjct: 1 MIEMPPLQFASSNGIRIGYYDAGPATDTPPLVLCHGWPELAFSWRHQIKALSEAGIRVIA 60 Query: 61 PDQRGYGATDRPEPVEAYDMEHLTGDLVGLLDHLKIDKAIFVGHDWGGFVVWQMPLRHPA 120 PDQRG+GA+DRP+PVEAYD+E LTGDLVGLLDHL IDKAIFVGHDWGGFVVWQMPLRHP Sbjct: 61 PDQRGFGASDRPQPVEAYDIEQLTGDLVGLLDHLAIDKAIFVGHDWGGFVVWQMPLRHPT 120 Query: 121 RVAGVVGVNTPHWDRAPMDPIALFRQRFGDHMYIVQFQDPGREPDRIFGSRVEQTFDAFM 180 RVAGVVG+NTPH R P DP+A+FR+RFGD MYIVQFQDP REPDRIF SRVEQTFD FM Sbjct: 121 RVAGVVGINTPHTARPPNDPVAIFRKRFGDSMYIVQFQDPAREPDRIFASRVEQTFDVFM 180 Query: 181 RKP---AAGVGAS---------ARLNLAFPQMIANYDARHDPRTPILSADEKKVFVDTFT 228 +KP GV A A+ NLAFPQM+ YDA D R PILS EK+VFVDT++ Sbjct: 181 KKPLPRKQGVVAEGLAAADGDAAKPNLAFPQMVLAYDASRDTREPILSPQEKQVFVDTYS 240 Query: 229 KTGFTGGINWYRNFTRNWERSAGLDHHVRVPSLMIMAENDAVLPPSAADGMERLVPDLEK 288 TGFTGGINWYRN TRNW+RSA LD VRVPSLMIMAENDAVLPPSAADGME+LVPDLEK Sbjct: 241 ATGFTGGINWYRNMTRNWQRSADLDLIVRVPSLMIMAENDAVLPPSAADGMEKLVPDLEK 300 Query: 289 YLVKDSGHWTQQEKPDEVSAKLIEWRRKRFG 319 YLV+D GHWTQQEKPDEVSAKLIEWRR+RFG Sbjct: 301 YLVRDCGHWTQQEKPDEVSAKLIEWRRRRFG 331 >gb|ACO95125.1| epoxide hydrolase [uncultured organism] Length = 339 Score = 536 bits (1381), Expect = e-150, Method: Compositional matrix adjust. Identities = 252/335 (75%), Positives = 285/335 (85%), Gaps = 16/335 (4%) Query: 1 MIEMPPLQFAETNGIRMGFYEAGPKTDTPPVMLCHGWPELAFSWRHQIKALGEAGIRVIA 60 M++MPPLQ+A NGIRMGFYEAGPKTDTPP++LCHGWPE+AFSWRHQIKAL E G+RVIA Sbjct: 5 MMDMPPLQYANVNGIRMGFYEAGPKTDTPPLVLCHGWPEIAFSWRHQIKALSETGLRVIA 64 Query: 61 PDQRGYGATDRPEPVEAYDMEHLTGDLVGLLDHLKIDKAIFVGHDWGGFVVWQMPLRHPA 120 PDQRGYGATDRPEPVEAYD+E+LT DLVGLLDHL IDKAIFVGHDWGGF+VWQMPLRHP+ Sbjct: 65 PDQRGYGATDRPEPVEAYDIENLTADLVGLLDHLNIDKAIFVGHDWGGFIVWQMPLRHPS 124 Query: 121 RVAGVVGVNTPHWDRAPMDPIALFRQRFGDHMYIVQFQDPGREPDRIFGSRVEQTFDAFM 180 RVAGV+GVNTPH R DPI L RQR+GDH+YI QFQ P REPDRIF VE+TFD FM Sbjct: 125 RVAGVIGVNTPHTPRTATDPIELLRQRYGDHLYIAQFQHPSREPDRIFADNVEKTFDFFM 184 Query: 181 RK----------------PAAGVGASARLNLAFPQMIANYDARHDPRTPILSADEKKVFV 224 RK PAAG+GAS +LN+AFPQM+A YD + DPR ILS +E KVFV Sbjct: 185 RKPMPQKQPSAADANAGPPAAGLGASPKLNMAFPQMVAGYDGKLDPREKILSPEEMKVFV 244 Query: 225 DTFTKTGFTGGINWYRNFTRNWERSAGLDHHVRVPSLMIMAENDAVLPPSAADGMERLVP 284 DTF +GFTGGINWYRN TRNWERSA +DH VRVPSLMIMAE+D+VLPPSA DGME++VP Sbjct: 245 DTFRGSGFTGGINWYRNMTRNWERSAHIDHTVRVPSLMIMAESDSVLPPSACDGMEQIVP 304 Query: 285 DLEKYLVKDSGHWTQQEKPDEVSAKLIEWRRKRFG 319 DLEKYLV++SGHWTQQE+PDEVSAK++EWRR+RFG Sbjct: 305 DLEKYLVRNSGHWTQQEQPDEVSAKILEWRRRRFG 339