BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0243.0046.p (360 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_769525.1| poly-beta-hydroxybutyrate polymerase [Bradyrhiz... 684 0.0 ref|ZP_08629150.1| polyhydroxyalkanoic acid synthase [Bradyrhizo... 629 e-178 ref|YP_004157584.1| alpha/beta hydrolase fold protein [Variovora... 518 e-145 ref|ZP_01615060.1| probable poly-beta-hydroxybutyrate polymerase... 450 e-124 ref|YP_001165937.1| alpha/beta hydrolase fold [Novosphingobium a... 404 e-111 >ref|NP_769525.1| poly-beta-hydroxybutyrate polymerase [Bradyrhizobium japonicum USDA 110] dbj|BAC48150.1| blr2885 [Bradyrhizobium japonicum USDA 110] Length = 360 Score = 684 bits (1766), Expect = 0.0, Method: Compositional matrix adjust. Identities = 321/360 (89%), Positives = 345/360 (95%) Query: 1 MNAPTPFDFASISERVQSEVQRAIQRSIKGVEYFSSSGPSLGSTPKDVLYSRGTMNLYHY 60 MNAPT DFASI ER+QSEVQRAIQRSIKGVEYFS+SGP+LGSTPKDVL+SRGTM+LYHY Sbjct: 1 MNAPTGLDFASIPERIQSEVQRAIQRSIKGVEYFSTSGPTLGSTPKDVLHSRGTMSLYHY 60 Query: 61 RPLASEVYRVPVLIVMATTNRGYILDLVPGQSFIEFLLKRGFDVYMLDWTAPKPEEKSLR 120 RP++ E+YRVPVLIVMATTNRGYILDLVPGQSFIEFLL+RG+DVYMLDW+AP+PEEKSLR Sbjct: 61 RPMSDEIYRVPVLIVMATTNRGYILDLVPGQSFIEFLLRRGYDVYMLDWSAPRPEEKSLR 120 Query: 121 MEDYVLDFIPESVRRVQQDSGEKDVSVIGYCFGGVLSLLYGSIFNDGPMKNLICFTTPID 180 MEDYVLDFIP+ VRRVQQDSGE+DVSVIGYCFGGVLSLLYGSI DGPMKNLICFTTPID Sbjct: 121 MEDYVLDFIPDCVRRVQQDSGEQDVSVIGYCFGGVLSLLYGSIHKDGPMKNLICFTTPID 180 Query: 181 FREMKLFSNFADRRYFDVDRLIDSTGNMPPELILQSFDMLRPAARVVGQIQLWDNIWNDE 240 FREMKLFSNF+DRRYFDVDRL+DS GN+PPE+IL SF+MLRPA+R V QIQLW+NIWNDE Sbjct: 181 FREMKLFSNFSDRRYFDVDRLVDSIGNVPPEMILSSFEMLRPASRTVSQIQLWENIWNDE 240 Query: 241 FVKSYRMFDRWATDTLPLAGEYFRAITKDLMWDNKLYNDTMTVGGREAKLADIKVPILHA 300 FVKSYRMFDRWATDTLPLAGEYFR ITKDLMWDNKL+NDTM+VGGR AKL DIKVPILHA Sbjct: 241 FVKSYRMFDRWATDTLPLAGEYFRTITKDLMWDNKLFNDTMSVGGRAAKLEDIKVPILHA 300 Query: 301 VAEHDHIVPYDAAKHLITKIGSADKEEVMLKGGHVSLVAGANAIKRLWPKLDSWLGKRST 360 VAEHDHIVPYDAAKHLI KIGSADKEEVMLKGGHVSLVAGANA+KRLWPKLDSWLGKRST Sbjct: 301 VAEHDHIVPYDAAKHLIAKIGSADKEEVMLKGGHVSLVAGANAVKRLWPKLDSWLGKRST 360 >ref|ZP_08629150.1| polyhydroxyalkanoic acid synthase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP08509.1| polyhydroxyalkanoic acid synthase [Bradyrhizobiaceae bacterium SG-6C] Length = 369 Score = 629 bits (1623), Expect = e-178, Method: Compositional matrix adjust. Identities = 290/358 (81%), Positives = 324/358 (90%) Query: 3 APTPFDFASISERVQSEVQRAIQRSIKGVEYFSSSGPSLGSTPKDVLYSRGTMNLYHYRP 62 A T DF I ER+Q+EVQRAIQRSIKGVEYF+SSGP+LGSTPKDVL SRGTMNLYHYRP Sbjct: 12 ASTVPDFTQIGERIQTEVQRAIQRSIKGVEYFASSGPALGSTPKDVLISRGTMNLYHYRP 71 Query: 63 LASEVYRVPVLIVMATTNRGYILDLVPGQSFIEFLLKRGFDVYMLDWTAPKPEEKSLRME 122 +A E+YR+P+LIVMATTNRGYILDLVPGQSFIEFLLK+GFDVYMLDW AP+PEEKSLRME Sbjct: 72 MADEIYRIPILIVMATTNRGYILDLVPGQSFIEFLLKKGFDVYMLDWNAPRPEEKSLRME 131 Query: 123 DYVLDFIPESVRRVQQDSGEKDVSVIGYCFGGVLSLLYGSIFNDGPMKNLICFTTPIDFR 182 DYVL FIPE ++RVQ+DSGE DV++IGYCFGGVLSLLY SI DGP+KNL+CFTTPIDFR Sbjct: 132 DYVLGFIPECIQRVQEDSGETDVNLIGYCFGGVLSLLYSSIHRDGPVKNLVCFTTPIDFR 191 Query: 183 EMKLFSNFADRRYFDVDRLIDSTGNMPPELILQSFDMLRPAARVVGQIQLWDNIWNDEFV 242 +MKLF NF+DRRYFDVD LID GN+ PE+I SF+MLRPA+R VGQIQLW+NIWNDE+V Sbjct: 192 QMKLFQNFSDRRYFDVDGLIDKAGNVSPEMIRSSFEMLRPASRAVGQIQLWENIWNDEYV 251 Query: 243 KSYRMFDRWATDTLPLAGEYFRAITKDLMWDNKLYNDTMTVGGREAKLADIKVPILHAVA 302 KS++MFDRW DTLPLAGEYFR TKDLMWDNKL+ND MTVGGR A LADIK+P+LHAVA Sbjct: 252 KSFQMFDRWGQDTLPLAGEYFRQTTKDLMWDNKLFNDAMTVGGRAANLADIKIPLLHAVA 311 Query: 303 EHDHIVPYDAAKHLITKIGSADKEEVMLKGGHVSLVAGANAIKRLWPKLDSWLGKRST 360 EHDHIVPYDAAKHL+ KIGS DK EV+LKGGHVS+VAGANAIKRLWPKLDSWL +RST Sbjct: 312 EHDHIVPYDAAKHLVAKIGSIDKTEVVLKGGHVSVVAGANAIKRLWPKLDSWLSERST 369 >ref|YP_004157584.1| alpha/beta hydrolase fold protein [Variovorax paradoxus EPS] gb|ADU39473.1| alpha/beta hydrolase fold protein [Variovorax paradoxus EPS] Length = 357 Score = 518 bits (1333), Expect = e-145, Method: Compositional matrix adjust. Identities = 230/345 (66%), Positives = 290/345 (84%) Query: 16 VQSEVQRAIQRSIKGVEYFSSSGPSLGSTPKDVLYSRGTMNLYHYRPLASEVYRVPVLIV 75 + +E++RAIQR++KG+++ +S+ P++G T +D ++ RGT++LYHYRPL E+YRVP+LIV Sbjct: 13 IGAEIERAIQRNLKGLDFLTSAAPAMGQTARDEIHRRGTLSLYHYRPLVDEIYRVPLLIV 72 Query: 76 MATTNRGYILDLVPGQSFIEFLLKRGFDVYMLDWTAPKPEEKSLRMEDYVLDFIPESVRR 135 MATTNRGYILDL PGQS +E LL +G+DVYM+DW AP+P+EK LR+EDYVLDFIP+S+RR Sbjct: 73 MATTNRGYILDLAPGQSMVEHLLLQGYDVYMMDWNAPRPQEKQLRIEDYVLDFIPDSLRR 132 Query: 136 VQQDSGEKDVSVIGYCFGGVLSLLYGSIFNDGPMKNLICFTTPIDFREMKLFSNFADRRY 195 VQ SGE+DV+++GYC GGVLS LY ++ +GP+KNL FTTPIDF MKLF ++DRRY Sbjct: 133 VQARSGEEDVTLVGYCMGGVLSTLYAALHPEGPIKNLALFTTPIDFSRMKLFHAWSDRRY 192 Query: 196 FDVDRLIDSTGNMPPELILQSFDMLRPAARVVGQIQLWDNIWNDEFVKSYRMFDRWATDT 255 FDVDRLID+ GN+PPE++L SFDMLRPA R G +QLWDN+ NDEFVKSYRMFDRW + Sbjct: 193 FDVDRLIDTIGNVPPEMLLASFDMLRPAGRSAGVVQLWDNMANDEFVKSYRMFDRWGAEM 252 Query: 256 LPLAGEYFRAITKDLMWDNKLYNDTMTVGGREAKLADIKVPILHAVAEHDHIVPYDAAKH 315 LPLAGEYFR K+LMWDNKL+ + +GGR+ LA+IKVP+LHA+A+HDHIVPY+A + Sbjct: 253 LPLAGEYFRQTVKELMWDNKLFKGELVLGGRKVLLANIKVPVLHALAQHDHIVPYEAGQP 312 Query: 316 LITKIGSADKEEVMLKGGHVSLVAGANAIKRLWPKLDSWLGKRST 360 LI IGS DKEEV+LKGGHVSL AGANA++RLWPKLD WLG RST Sbjct: 313 LIANIGSTDKEEVVLKGGHVSLAAGANAVRRLWPKLDDWLGVRST 357 >ref|ZP_01615060.1| probable poly-beta-hydroxybutyrate polymerase [marine gamma proteobacterium HTCC2143] gb|EAW32143.1| probable poly-beta-hydroxybutyrate polymerase [marine gamma proteobacterium HTCC2143] Length = 359 Score = 450 bits (1158), Expect = e-124, Method: Compositional matrix adjust. Identities = 192/340 (56%), Positives = 266/340 (78%) Query: 20 VQRAIQRSIKGVEYFSSSGPSLGSTPKDVLYSRGTMNLYHYRPLASEVYRVPVLIVMATT 79 V + IQR +KGV++ S ++G +PKD++Y RGT+ LYHY P++ EVYR+PV++VMATT Sbjct: 19 VDKTIQRGVKGVQFARSGKANVGQSPKDLIYDRGTLKLYHYHPMSDEVYRIPVILVMATT 78 Query: 80 NRGYILDLVPGQSFIEFLLKRGFDVYMLDWTAPKPEEKSLRMEDYVLDFIPESVRRVQQD 139 NRGYI DL+PGQS +E+LLKRGFDVYM+DW P P E+ L + DY DFIP V RVQQ+ Sbjct: 79 NRGYIFDLLPGQSMVEYLLKRGFDVYMIDWDPPMPSERGLSLADYTQDFIPSCVERVQQE 138 Query: 140 SGEKDVSVIGYCFGGVLSLLYGSIFNDGPMKNLICFTTPIDFREMKLFSNFADRRYFDVD 199 SGE+DV++I YC GGVLSL+Y + DGP+KNL C TTPI+++EM LF+N+ ++++ ++D Sbjct: 139 SGEEDVNIIAYCMGGVLSLIYAATHLDGPVKNLACLTTPINWQEMGLFNNWTNQQHLNLD 198 Query: 200 RLIDSTGNMPPELILQSFDMLRPAARVVGQIQLWDNIWNDEFVKSYRMFDRWATDTLPLA 259 +L+D+ G +P + I +SF MLRPAA+ GQ++LWD +WNDEFV SYR F+RW +T+ L Sbjct: 199 KLVDTLGVIPADFIERSFSMLRPAAKTAGQLRLWDQMWNDEFVTSYRAFERWGNETIGLP 258 Query: 260 GEYFRAITKDLMWDNKLYNDTMTVGGREAKLADIKVPILHAVAEHDHIVPYDAAKHLITK 319 G Y++ T++LMW NKL+ + + V G+ AKL++IK+P++H VAEHDHI Y A K L+ Sbjct: 259 GTYYKETTQELMWGNKLFTEELIVAGKPAKLSEIKIPLMHIVAEHDHIATYGATKPLLEL 318 Query: 320 IGSADKEEVMLKGGHVSLVAGANAIKRLWPKLDSWLGKRS 359 GS DKEEV+LKGGHVSL+AG NA++R+WPK++ WLG+RS Sbjct: 319 AGSEDKEEVVLKGGHVSLIAGPNAVRRMWPKINEWLGERS 358 >ref|YP_001165937.1| alpha/beta hydrolase fold [Novosphingobium aromaticivorans DSM 12444] gb|ABP64411.1| alpha/beta hydrolase fold [Novosphingobium aromaticivorans DSM 12444] Length = 360 Score = 404 bits (1039), Expect = e-111, Method: Compositional matrix adjust. Identities = 180/360 (50%), Positives = 259/360 (71%) Query: 1 MNAPTPFDFASISERVQSEVQRAIQRSIKGVEYFSSSGPSLGSTPKDVLYSRGTMNLYHY 60 M P +++ERV+ EV RA++R+IKG+E+ ++ +G+ K++++ GT LY Y Sbjct: 1 MAESDPLSPEAVAERVRREVDRALKRNIKGLEFLAAPRQEVGAMEKELVHREGTALLYRY 60 Query: 61 RPLASEVYRVPVLIVMATTNRGYILDLVPGQSFIEFLLKRGFDVYMLDWTAPKPEEKSLR 120 RP+ E+YR+P+LIV +N+GYI DL PGQS +E+LL RG+DV+ LDW P+ +E L Sbjct: 61 RPVVDEIYRLPLLIVSPPSNKGYIFDLAPGQSMVEYLLNRGYDVFNLDWAPPRRDEAHLG 120 Query: 121 MEDYVLDFIPESVRRVQQDSGEKDVSVIGYCFGGVLSLLYGSIFNDGPMKNLICFTTPID 180 + DYV FIP+S+ ++ + +GE++ S+ GYC GG L+ ++ ++ ++NL+ F TP+D Sbjct: 121 LADYVDRFIPDSLAKIAEITGEEEASLAGYCMGGTLATIHAALHAGNGVRNLVLFATPVD 180 Query: 181 FREMKLFSNFADRRYFDVDRLIDSTGNMPPELILQSFDMLRPAARVVGQIQLWDNIWNDE 240 F M LF ++D+R+FDVD ++D G +PPE++L +FD+ RPA R G++ LW N+WNDE Sbjct: 181 FTHMHLFQKWSDKRHFDVDEVVDKLGVIPPEIMLGAFDLARPANRTAGRMHLWTNMWNDE 240 Query: 241 FVKSYRMFDRWATDTLPLAGEYFRAITKDLMWDNKLYNDTMTVGGREAKLADIKVPILHA 300 FV+SYRMFDRWA +TLP+ GEYFR K LMWDN L + T+ VGGR+A LA+I VPIL+ Sbjct: 241 FVRSYRMFDRWAAETLPVPGEYFREQIKHLMWDNALASGTLEVGGRKADLANIAVPILNV 300 Query: 301 VAEHDHIVPYDAAKHLITKIGSADKEEVMLKGGHVSLVAGANAIKRLWPKLDSWLGKRST 360 VA+HDHIV ++A + L S D EE++ KGGHVSLVAG +A+KRLWP +D WLGKRS Sbjct: 301 VAQHDHIVSHEATQPLKLLTSSDDYEEIVSKGGHVSLVAGPSALKRLWPLIDGWLGKRSV 360