BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0241.0043.p (200 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_767568.1| protocatechuate 3,4-dioxygenase subunit alpha [... 311 3e-83 ref|ZP_08630876.1| protocatechuate 3,4-dioxygenase alpha chain [... 292 1e-77 ref|ZP_02188807.1| Protocatechuate 3,4-dioxygenase, alpha subuni... 207 5e-52 ref|YP_003318863.1| protocatechuate 3,4-dioxygenase, alpha subun... 207 8e-52 gb|AAW29746.1| sulfocatechol 3,4-dioxygenase alpha subunit [Novo... 195 2e-48 >ref|NP_767568.1| protocatechuate 3,4-dioxygenase subunit alpha [Bradyrhizobium japonicum USDA 110] dbj|BAC46193.1| protocatechuate 3,4-dioxygenase alpha chain [Bradyrhizobium japonicum USDA 110] Length = 200 Score = 311 bits (798), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 146/196 (74%), Positives = 166/196 (84%), Gaps = 1/196 (0%) Query: 5 RPDGITPSQTVGPYFKYGLTPNGEYEWNDAFTNNLLTPDTSGERIRVEGKVYDGDGAVIP 64 +P+GITPSQTVGP+FKYGLTP G+Y WNDAFTN+ LTPD +G+R+ +EG+V DGDG +P Sbjct: 6 KPEGITPSQTVGPFFKYGLTPTGDYAWNDAFTNSTLTPDVTGDRVHIEGRVLDGDGVAVP 65 Query: 65 DCMLEIWQADAQGRFSDPQDARALPNSTFKGFGRCGTDKNGAYAFDTIKPGVVPDADGKP 124 D MLEIWQADAQGRF+DPQD RALPN++F+GF RCGTDK+G YAFDT+KPG VPD DGK Sbjct: 66 DAMLEIWQADAQGRFADPQDKRALPNASFRGFARCGTDKDGNYAFDTVKPGAVPDLDGKL 125 Query: 125 QAPHILLAVFARGMLLHLYTRIYFDDEAGNADDSTLALVPADRRATLIARRKAGVGNTYS 184 QAPHILLAVF RGML HLYTRIYF DEAGNA D LALVP +RRATLIA R+AG Y Sbjct: 126 QAPHILLAVFGRGMLRHLYTRIYFSDEAGNAADPVLALVPTERRATLIATREAGKA-VYR 184 Query: 185 FDVHLQGDNETVFFDV 200 D+ LQGDNETVFFDV Sbjct: 185 LDLRLQGDNETVFFDV 200 >ref|ZP_08630876.1| protocatechuate 3,4-dioxygenase alpha chain [Bradyrhizobiaceae bacterium SG-6C] gb|EGP06408.1| protocatechuate 3,4-dioxygenase alpha chain [Bradyrhizobiaceae bacterium SG-6C] Length = 195 Score = 292 bits (748), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 137/192 (71%), Positives = 156/192 (81%) Query: 9 ITPSQTVGPYFKYGLTPNGEYEWNDAFTNNLLTPDTSGERIRVEGKVYDGDGAVIPDCML 68 ITPSQTVGPYFKYGLTP +Y WNDAF+++L+TPD SGERIR+ G+V+DG+G VIPD ML Sbjct: 4 ITPSQTVGPYFKYGLTPGSDYAWNDAFSSDLVTPDVSGERIRIVGQVFDGEGKVIPDSML 63 Query: 69 EIWQADAQGRFSDPQDARALPNSTFKGFGRCGTDKNGAYAFDTIKPGVVPDADGKPQAPH 128 EIWQAD+QGRF+DPQD RA+ N+ FKGFGRCGTD NG + F TI+PG VP GKPQAPH Sbjct: 64 EIWQADSQGRFADPQDTRAVANAAFKGFGRCGTDANGGFEFRTIRPGSVPGPGGKPQAPH 123 Query: 129 ILLAVFARGMLLHLYTRIYFDDEAGNADDSTLALVPADRRATLIARRKAGVGNTYSFDVH 188 ILLAVFARGM TRIYF DE+ NADD LALVPA+RRATLIA R G Y FD+H Sbjct: 124 ILLAVFARGMTQQAITRIYFADESANADDPILALVPAERRATLIASRAQGDAAMYRFDIH 183 Query: 189 LQGDNETVFFDV 200 LQGD ETVFFD+ Sbjct: 184 LQGDRETVFFDL 195 >ref|ZP_02188807.1| Protocatechuate 3,4-dioxygenase, alpha subunit(PcaG) [alpha proteobacterium BAL199] gb|EDP64635.1| Protocatechuate 3,4-dioxygenase, alpha subunit(PcaG) [alpha proteobacterium BAL199] Length = 198 Score = 207 bits (528), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 115/197 (58%), Positives = 132/197 (67%), Gaps = 10/197 (5%) Query: 8 GITPSQTVGPYFKYGLTPNGEYEWNDAFTNNLLTPDTS-GERIRVEGKVYDGDGAVIPDC 66 G TPSQTVGPYF YGLTP A L DTS GERIR+EG+V+DG+GAV+ D Sbjct: 6 GQTPSQTVGPYFAYGLTPEQYGYPLKAIAGGRLADDTSEGERIRIEGQVFDGNGAVVADA 65 Query: 67 MLEIWQADAQGRFSDPQDARALPNSTFKGFGR--CGTDKNGAYAFDTIKPGVVPDADGKP 124 M+EIWQADAQGR++ P D R NS F+GFGR GTD + FDT+KPG V D Sbjct: 66 MIEIWQADAQGRYAHPADPRG-SNSDFRGFGRFGTGTDPKNRFIFDTVKPGAVGDG---- 120 Query: 125 QAPHILLAVFARGMLLHLYTRIYFDDEA-GNADDSTLALVPADRRATLIARRKAG-VGNT 182 QAPHI + VF RGMLLH +TR+YF DE NA D L VP RR TLIARR+ G G Sbjct: 121 QAPHINVIVFMRGMLLHTFTRLYFSDETEANASDPVLRTVPDSRRETLIARRQDGPTGTV 180 Query: 183 YSFDVHLQGDNETVFFD 199 Y FD+H+QGD ETVFFD Sbjct: 181 YRFDIHMQGDAETVFFD 197 >ref|YP_003318863.1| protocatechuate 3,4-dioxygenase, alpha subunit [Sphaerobacter thermophilus DSM 20745] gb|ACZ38041.1| protocatechuate 3,4-dioxygenase, alpha subunit [Sphaerobacter thermophilus DSM 20745] Length = 193 Score = 207 bits (526), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 109/194 (56%), Positives = 132/194 (68%), Gaps = 8/194 (4%) Query: 9 ITPSQTVGPYFKYGLTPNGEYEWNDAFTNNLLTPDTSGERIRVEGKVYDGDGAVIPDCML 68 +TP+QTVGP+F L DA L+ P+T G RIR+EG+V DGDG + D M+ Sbjct: 6 VTPTQTVGPFFHPCLL------RPDAVITTLVKPETEGTRIRIEGRVLDGDGLPVDDAMV 59 Query: 69 EIWQADAQGRFSDPQDARALP-NSTFKGFGRCGTDKNGAYAFDTIKPGVVPDADGKPQAP 127 EIWQA+ GR+ P D R LP + TF GFGR GTD+ G Y F+TIKPGVVP D + QAP Sbjct: 60 EIWQANHYGRYRHPADQRPLPLDPTFIGFGRTGTDEEGRYWFETIKPGVVPFDDQQDQAP 119 Query: 128 HILLAVFARGMLLHLYTRIYFDDEAGNADDSTLALVPADRRATLIARRKAGVGN-TYSFD 186 HIL+ VFARG+L H+ TRIYF+DE NA D L VP +RR TLIARR+ GN Y FD Sbjct: 120 HILVMVFARGLLNHVVTRIYFEDEPANATDPILLRVPEERRGTLIARREEVDGNVVYRFD 179 Query: 187 VHLQGDNETVFFDV 200 + LQG+ ETVFFDV Sbjct: 180 IRLQGEGETVFFDV 193 >gb|AAW29746.1| sulfocatechol 3,4-dioxygenase alpha subunit [Novosphingobium resinovorum] Length = 196 Score = 195 bits (496), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 99/199 (49%), Positives = 128/199 (64%), Gaps = 4/199 (2%) Query: 1 VSNPRPDGITPSQTVGPYFKYGLTPNGEYEWNDAFTNNLLTPDTSGERIRVEGKVYDGDG 60 ++ P ITPSQTVGP++ Y LTP F L T D GERI ++G + DG+G Sbjct: 1 MTTKTPLTITPSQTVGPFYAYCLTPEDYGTLPPLFGAQLATEDAEGERITIQGTITDGEG 60 Query: 61 AVIPDCMLEIWQADAQGRFSDPQDARALPNSTFKGFGRCGTDKNGAYAFDTIKPGVVPDA 120 A++PD ++EIWQ D QGRF+ L NS FKGFGR DK+G ++F T+KPG VP A Sbjct: 61 AMVPDALIEIWQPDGQGRFAGAHPE--LRNSAFKGFGRRHCDKSGNFSFQTVKPGRVPTA 118 Query: 121 DGKPQAPHILLAVFARGMLLHLYTRIYFDDEAGNADDSTLALVPADRRATLIARRKAGVG 180 DG QAPHI L++F +G+ LYTRIYF DEA NA+D L+++ D R TLIA ++ Sbjct: 119 DGVMQAPHIALSIFGKGLNRRLYTRIYFADEASNAEDPVLSMLSEDERVTLIATSESPAA 178 Query: 181 NTYSFDVHLQGDNETVFFD 199 Y D+ LQGD ETVFF+ Sbjct: 179 --YRLDIRLQGDGETVFFE 195