BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0020.p (432 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_06888893.1| major facilitator superfamily MFS_1 [Methylos... 545 e-153 ref|ZP_08074305.1| major facilitator superfamily MFS_1 [Methyloc... 525 e-147 ref|YP_113110.1| nitrate transporter [Methylococcus capsulatus s... 499 e-139 ref|YP_002364015.1| major facilitator superfamily protein [Methy... 398 e-108 ref|YP_284051.1| nitrate transporter [Dechloromonas aromatica RC... 395 e-108 >ref|ZP_06888893.1| major facilitator superfamily MFS_1 [Methylosinus trichosporium OB3b] gb|EFH02617.1| major facilitator superfamily MFS_1 [Methylosinus trichosporium OB3b] Length = 418 Score = 545 bits (1405), Expect = e-153, Method: Compositional matrix adjust. Identities = 306/419 (73%), Positives = 349/419 (83%), Gaps = 1/419 (0%) Query: 14 MKLAEFKKAGHWPTLLAAFLYFDISFMAWVALGPLIVYIARDMNLAVNEKFTLVAIPVLA 73 MKL +F+KAGHWPTLLAAFLYFD+SFMAWV+LGPL++YIA+DM L+V +K +LVAIPVL Sbjct: 1 MKLDDFEKAGHWPTLLAAFLYFDVSFMAWVSLGPLMIYIAKDMGLSVEDKLSLVAIPVLG 60 Query: 74 GALLRVPLGILADIIGAKRTGIIAQLVVIAATAAVWHFGLTSKLAIELFGLALGVGGASF 133 GA RVPLG+LAD +GAK TG+IAQ VVI A + V FGLT LAI LFG++LGV GASF Sbjct: 61 GAFFRVPLGLLADAVGAKITGVIAQAVVIFAVSLVCVFGLTGPLAIALFGISLGVAGASF 120 Query: 134 AVALPQASRWYPPQYQGVVMGIAGAGNMGVVLDTLLAPSIAEHWGWQAVFGCLLIPMLMV 193 AVALPQA RWYPPQYQGVVMGIAGAGNMGVVLDTLLAP+IAE +GW+AV+ LL M +V Sbjct: 121 AVALPQAGRWYPPQYQGVVMGIAGAGNMGVVLDTLLAPTIAETYGWRAVYCVLLALMALV 180 Query: 194 LAYYAFAAKDAPGERKKVSLQAYGALLKDSDSRWFMFFYFITFGGFVGLANALPLYFTVQ 253 L Y AAKDAP +K ++L YG LL DSDSRWFMFFYFITFGGFVGLA+ALPLYFT Q Sbjct: 181 LIVYCAAAKDAPVAKKAITLADYGRLLTDSDSRWFMFFYFITFGGFVGLASALPLYFTTQ 240 Query: 254 YHVSGVAAGMLVALIVAFGSGFRPVGGMIADRIGGIRSLSILFGVVVAAYLVIAFMPEGP 313 YH +GVAAG++VALIVAFGSGFRPVGG IADRIGG+R+L+ LF VV A Y IA +PEGP Sbjct: 241 YHATGVAAGLVVALIVAFGSGFRPVGGYIADRIGGVRTLTALFVVVSACYFAIALLPEGP 300 Query: 314 TAPTPAGWALIELPRIAWISVLLFSIGVLALGMGNGAVFQLIPLRFRHEIGLMTGLVGCA 373 AP GW+L ELP IA+ +V LFS+GVLALGMGNGAVFQL+P RFR EIG MTGLVG A Sbjct: 301 -APAKGGWSLTELPPIAFGAVALFSLGVLALGMGNGAVFQLLPQRFRAEIGAMTGLVGAA 359 Query: 374 GGIGGFFLAQTLGFAKGMTGGFGAGFLFFGGLALLGFIGLAMVKVRWRTTWGAVSGARV 432 GG+GG+FLA+TL +KGMTGGF GF FF LALLG +GL VK RWRTTWGAVSGAR+ Sbjct: 360 GGLGGYFLAKTLAVSKGMTGGFTIGFSFFSLLALLGLVGLVFVKTRWRTTWGAVSGARI 418 >ref|ZP_08074305.1| major facilitator superfamily MFS_1 [Methylocystis sp. ATCC 49242] gb|EFX98019.1| major facilitator superfamily MFS_1 [Methylocystis sp. ATCC 49242] Length = 420 Score = 525 bits (1353), Expect = e-147, Method: Compositional matrix adjust. Identities = 298/420 (70%), Positives = 344/420 (81%), Gaps = 1/420 (0%) Query: 14 MKLAEFKKAGHWPTLLAAFLYFDISFMAWVALGPLIVYIARDMNLAVNEKFTLVAIPVLA 73 MKL +FKKAGHWPTLLAAFLYFDISFMAWV+LGPL+VYI +DM++ V +K +LVAIPVL Sbjct: 1 MKLDDFKKAGHWPTLLAAFLYFDISFMAWVSLGPLMVYITKDMHIPVEDKLSLVAIPVLG 60 Query: 74 GALLRVPLGILADIIGAKRTGIIAQLVVIAATAAVWHFGLTSKLAIELFGLALGVGGASF 133 GA+ R+PLGILAD IG+KR GI AQ++VI A + V FGLT AI LFG++LGV GASF Sbjct: 61 GAIFRMPLGILADTIGSKRAGIAAQVIVIFAVSLVAVFGLTCPAAIALFGVSLGVAGASF 120 Query: 134 AVALPQASRWYPPQYQGVVMGIAGAGNMGVVLDTLLAPSIAEHWGWQAVFGCLLIPMLMV 193 AVALPQA RWYPPQYQGVVMGIAGAGNMGVVLDTL AP IAE +GW+AV+G LL M++V Sbjct: 121 AVALPQAGRWYPPQYQGVVMGIAGAGNMGVVLDTLFAPWIAETFGWRAVYGVLLALMVLV 180 Query: 194 LAYYAFAAKDAPGERKKVSLQAYGALLKDSDSRWFMFFYFITFGGFVGLANALPLYFTVQ 253 YA A+D PG + +SL YG +L DSDSRWFMFFYFITFGGFVGLA+ALPLYFT Q Sbjct: 181 AVIYAIIARDPPGAKTTISLSDYGKILLDSDSRWFMFFYFITFGGFVGLASALPLYFTTQ 240 Query: 254 YHVSGVAAGMLVALIVAFGSGFRPVGGMIADRIGGIRSLSILFGVVVAAYLVIAFM-PEG 312 YH +GVAAG++VALIVAFGSGFRPVGG IADRIGG+++LS LF +V A Y IAF+ Sbjct: 241 YHANGVAAGLMVALIVAFGSGFRPVGGYIADRIGGVKTLSFLFLIVSACYFAIAFLPAGP 300 Query: 313 PTAPTPAGWALIELPRIAWISVLLFSIGVLALGMGNGAVFQLIPLRFRHEIGLMTGLVGC 372 A GW L ELP+IA+++V LFS+GVL LGMGNGAVFQL+P RFR EIG MTGLVG Sbjct: 301 APAAGAPGWRLTELPQIAFVAVALFSLGVLCLGMGNGAVFQLLPQRFRREIGAMTGLVGA 360 Query: 373 AGGIGGFFLAQTLGFAKGMTGGFGAGFLFFGGLALLGFIGLAMVKVRWRTTWGAVSGARV 432 AGG+GGFFLA+TL F+KG T F AGF+FFG LALLG IGL +VK RWRTTWGAVSGAR+ Sbjct: 361 AGGLGGFFLAKTLAFSKGATDNFAAGFMFFGLLALLGLIGLLVVKTRWRTTWGAVSGARI 420 >ref|YP_113110.1| nitrate transporter [Methylococcus capsulatus str. Bath] gb|AAU93153.1| putative nitrate transporter [Methylococcus capsulatus str. Bath] Length = 424 Score = 499 bits (1286), Expect = e-139, Method: Compositional matrix adjust. Identities = 281/425 (66%), Positives = 335/425 (78%), Gaps = 7/425 (1%) Query: 14 MKLAEFKKAGHWPTLLAAFLYFDISFMAWVALGPLIVYIARDMNLAVNEKFTLVAIPVLA 73 M L EFK+AGHWPTLL+AFLYFD+SFM WV LGPL +YI +D+ L + EKFT+VAIP+L+ Sbjct: 1 MNLKEFKQAGHWPTLLSAFLYFDVSFMVWVMLGPLSLYITKDLALPLEEKFTIVAIPILS 60 Query: 74 GALLRVPLGILADIIGAKRTGIIAQLVVIAATAAVWHFGLTSKLAIELFGLALGVGGASF 133 GA+ R+ LG +AD +G K TGI+AQL+VI A V+ FGL SKL +EL GL LGV GASF Sbjct: 61 GAVFRIILGSMADHVGPKPTGILAQLLVILGLAYVFWFGLHSKLEVELLGLLLGVAGASF 120 Query: 134 AVALPQASRWYPPQYQGVVMGIAGAGNMGVVLDTLLAPSIAEHWGWQAVFGCLLIPMLMV 193 AVALPQASRWYPP++QGVVMGIAGAGNMGVVLD+++ P +AE +GWQAVFG LLIP+++V Sbjct: 121 AVALPQASRWYPPKFQGVVMGIAGAGNMGVVLDSMIVPWLAEKFGWQAVFGFLLIPLVIV 180 Query: 194 LAYYAFAAKDAPGERKKVSLQAYGALLKDSDSRWFMFFYFITFGGFVGLANALPLYFTVQ 253 L YA KDAP +R V+L Y +++KD D+ WFMFFY ITFGGFVGL NALPLYFT Sbjct: 181 LTVYAVLVKDAPDKRAPVTLANYASVMKDPDAWWFMFFYSITFGGFVGLGNALPLYFTHW 240 Query: 254 YHVSGVAAGMLVALIVAFGSGFRPVGGMIADRIGGIRSLSILFGVVVAAYLVIAFMPEGP 313 YHVSG+AAGM+VA++V GS FRPVGG +ADRIGGIR+L ILF VV AYL I+FMPEGP Sbjct: 241 YHVSGIAAGMMVAIVVFAGSMFRPVGGYLADRIGGIRALQILFVVVSLAYLAISFMPEGP 300 Query: 314 TAPTP------AGWALIELPRIAWISVLLFSIGVLALGMGNGAVFQLIPLRFRHEIGLMT 367 AP AGW+L ELP W +V +F G LALGMGNG+VFQL+PLRFR EIG++T Sbjct: 301 -APEALGTAKVAGWSLGELPAQGWGAVAVFFAGTLALGMGNGSVFQLVPLRFRKEIGVVT 359 Query: 368 GLVGCAGGIGGFFLAQTLGFAKGMTGGFGAGFLFFGGLALLGFIGLAMVKVRWRTTWGAV 427 GLVGCAGG+GGFFLA+TLG + + GF GF F L LLG GL +V+ RWRTTWGAV Sbjct: 360 GLVGCAGGVGGFFLAKTLGLSWEIQHGFALGFSVFAVLPLLGLGGLMLVRKRWRTTWGAV 419 Query: 428 SGARV 432 SGARV Sbjct: 420 SGARV 424 >ref|YP_002364015.1| major facilitator superfamily protein [Methylocella silvestris BL2] gb|ACK52653.1| major facilitator superfamily MFS_1 [Methylocella silvestris BL2] Length = 404 Score = 398 bits (1022), Expect = e-108, Method: Compositional matrix adjust. Identities = 218/409 (53%), Positives = 275/409 (67%), Gaps = 17/409 (4%) Query: 18 EFKKAGHWPTLLAAFLYFDISFMAWVALGPLIVYIARDMNLAVNEKFTLVAIPVLAGALL 77 EF KAGH PTL AAF YFD++FM WV LGPL V IA D+ L +K +VAIP+LAGALL Sbjct: 5 EFFKAGHPPTLFAAFFYFDMAFMVWVLLGPLGVQIAGDLGLNAGQKGLMVAIPILAGALL 64 Query: 78 RVPLGILADIIGAKRTGIIAQLVVIAATAAVWHFGLTSKLAIELFGLALGVGGASFAVAL 137 RV +G++ D I K TG+I QL+VIA W G++S + G+ LGV GASFAVAL Sbjct: 65 RVVMGVMVDRIKPKLTGVIGQLIVIAGLLVAWSLGISSFGGVLALGVVLGVAGASFAVAL 124 Query: 138 PQASRWYPPQYQGVVMGIAGAGNMGVVLDTLLAPSIAEHWGWQAVFGCLLIPMLMVLAYY 197 P ASRWYPPQYQGV +GIAGAGN G VL L AP++A H+GW V G IP+ + L Y Sbjct: 125 PLASRWYPPQYQGVALGIAGAGNSGTVLAALFAPTLAAHFGWINVIGLAAIPVAIALVVY 184 Query: 198 AFAAKDAPGERKKVSLQAYGALLKDSDSRWFMFFYFITFGGFVGLANALPLYFTVQYHVS 257 AKDAP + S Y A+LK D+ WFMFFYF+TFGGF GLA++L +YF +Y +S Sbjct: 185 LLLAKDAPNQPAPKSFADYAAVLKLPDAWWFMFFYFVTFGGFSGLASSLTIYFNSEYGLS 244 Query: 258 GVAAGMLVALIVAFGSGFRPVGGMIADRIGGIRSLSILFGVVVAAYLVIAFMPEGPTAPT 317 VAAG A V GS RP+GG +ADR GG+R+L+I++ V AA +++F Sbjct: 245 PVAAGYATAACVFAGSLVRPIGGALADRTGGVRALTIMYCVAAAALTIVSF--------- 295 Query: 318 PAGWALIELPRIAWISVLLFSIGVLALGMGNGAVFQLIPLRFRHEIGLMTGLVGCAGGIG 377 LP++ ++ +F + +LALGMGNGAVFQL+P RFRHEIG+MTGLVG GG+G Sbjct: 296 -------RLPQMG-AALAVFVVAMLALGMGNGAVFQLVPQRFRHEIGVMTGLVGMTGGVG 347 Query: 378 GFFLAQTLGFAKGMTGGFGAGFLFFGGLALLGFIGLAMVKVRWRTTWGA 426 GF+LA +LG++K TG + G L F LA+ IGL +K RWRTTWGA Sbjct: 348 GFYLASSLGYSKQFTGTYQLGLLVFAILAVSALIGLTSIKRRWRTTWGA 396 >ref|YP_284051.1| nitrate transporter [Dechloromonas aromatica RCB] gb|AAZ45581.1| Nitrate transporter [Dechloromonas aromatica RCB] Length = 404 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 215/414 (51%), Positives = 277/414 (66%), Gaps = 19/414 (4%) Query: 14 MKLAEFKKAGHWPTLLAAFLYFDISFMAWVALGPLIVYIARDMNLAVNEKFTLVAIPVLA 73 M F +AGH PTLLAAFLYFD++FM WV LGPL + IA+D+ L+ EK +VA PVLA Sbjct: 1 MDKKSFLQAGHTPTLLAAFLYFDLAFMVWVLLGPLGISIAKDLGLSHAEKGMMVATPVLA 60 Query: 74 GALLRVPLGILADIIGAKRTGIIAQLVVIAATAAVWHFGLTSKLAIELFGLALGVGGASF 133 GALLR+ +GIL D + K+ II Q+VVIA W G+ S + + GL LGV GASF Sbjct: 61 GALLRIIMGILVDRLSPKKAAIIGQVVVIATMIYAWQVGVHSYAEVLILGLFLGVAGASF 120 Query: 134 AVALPQASRWYPPQYQGVVMGIAGAGNMGVVLDTLLAPSIAEHWGWQAVFGCLLIPMLMV 193 A ALP ASRWYP ++QG MGIAGAGN G L L AP +A +GW VFG +LIP+++V Sbjct: 121 AAALPLASRWYPAEHQGTAMGIAGAGNSGTALAALFAPGLAAAFGWVNVFGVVLIPLVIV 180 Query: 194 LAYYAFAAKDAPGERKKVSLQAYGALLKDSDSRWFMFFYFITFGGFVGLANALPLYFTVQ 253 L + F AKDAP + Y +LKD D+ WFMFFY +TFGGFVGLA++L +YF Q Sbjct: 181 LIAFIFMAKDAPDAPPPKTFAEYLKVLKDKDAWWFMFFYSVTFGGFVGLASSLVIYFNTQ 240 Query: 254 YHVSGVAAGMLVALIVAFGSGFRPVGGMIADRIGGIRSLSILFGVVVAAYLVIAF-MPEG 312 Y + AG A V GS RP+GG +ADRIGG++SL++++ +++F +PE Sbjct: 241 YGLDPKMAGYFTAGCVFAGSLVRPIGGNVADRIGGVKSLTVMYMFAALFLSIVSFGLPE- 299 Query: 313 PTAPTPAGWALIELPRIAWISVLLFSIGVLALGMGNGAVFQLIPLRFRHEIGLMTGLVGC 372 AW+++L+F +L+LGMGNGAVFQL+P RFR EIG+MTGLVG Sbjct: 300 -----------------AWMALLVFVGAMLSLGMGNGAVFQLVPQRFRKEIGVMTGLVGM 342 Query: 373 AGGIGGFFLAQTLGFAKGMTGGFGAGFLFFGGLALLGFIGLAMVKVRWRTTWGA 426 AGG+GGF+LA +LG++K +TG + AGFL F LA+L +GL VK RWRTTWGA Sbjct: 343 AGGVGGFYLASSLGYSKQLTGSYQAGFLIFASLAVLALLGLTGVKTRWRTTWGA 396