BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0010.p (279 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001372550.1| enoyl-CoA hydratase [Ochrobactrum anthropi A... 460 e-127 ref|YP_933436.1| enoyl-CoA hydratase [Azoarcus sp. BH72] >gi|119... 436 e-120 gb|AAL02065.1|AF320253_3 putative enoyl-CoA hydratase/isomerase ... 436 e-120 gb|AAL02073.1|AF320254_3 putative enoyl-CoA hydratase/isomerase ... 431 e-119 ref|YP_001526104.1| enoyl-CoA hydratase [Azorhizobium caulinodan... 426 e-117 >ref|YP_001372550.1| enoyl-CoA hydratase [Ochrobactrum anthropi ATCC 49188] gb|ABS16721.1| Enoyl-CoA hydratase/isomerase [Ochrobactrum anthropi ATCC 49188] Length = 283 Score = 460 bits (1183), Expect = e-127, Method: Compositional matrix adjust. Identities = 217/276 (78%), Positives = 243/276 (88%) Query: 4 MKAKLRPFKDYPAKHFRWQVDASGRVATITLNRPDKKNPLTFESYEELRDLFTNLKYASD 63 M + L+PFKDY A HF WQ GRVAT+TLNRPD+KNPLTFESY ELRDLF NL YASD Sbjct: 8 MASNLKPFKDYQATHFLWQTSEDGRVATLTLNRPDRKNPLTFESYAELRDLFRNLVYASD 67 Query: 64 VRAIVLTGAEGNFCSGGDVFEIIEPLTRMAMPDLLAFTRMTGEVVRAMRKCPQIIVAAID 123 VR IVLTGA GNF SGGDVFEIIEPLT M MPDLLAFTRMTG+VV+AMRKCPQ I+AA+D Sbjct: 68 VRTIVLTGAGGNFSSGGDVFEIIEPLTHMTMPDLLAFTRMTGDVVKAMRKCPQQIIAAVD 127 Query: 124 GICAGAGAMLALASDLRLATPQAKTAFLFTRVGLAGADMGACGLLPRVIGQGHAADLLYT 183 GICAGAGA+LA+ASDLR+ATP+AKTAFLFTRVGLAGADMGACG+LPR+IGQG A++LLYT Sbjct: 128 GICAGAGAILAMASDLRIATPEAKTAFLFTRVGLAGADMGACGILPRLIGQGRASELLYT 187 Query: 184 GRAMSAEEGFAWGFHNRLVPPAELAGAAQDLARSLADGPWFAHGVTKTMFNQEWAMGVDE 243 GR MSA+EG WGF N L P +L+G A+ LARS+ADGPWFAHG+TKTM NQEWAMG++E Sbjct: 188 GRFMSADEGERWGFFNALHPQGDLSGKAEALARSIADGPWFAHGMTKTMLNQEWAMGIEE 247 Query: 244 MIESEAQAQAICMVTGDFRRAFEAFAAKQKPAFEGN 279 ++ESEAQAQAICM T DFRRAFEAFAAKQKP FEGN Sbjct: 248 LVESEAQAQAICMATQDFRRAFEAFAAKQKPVFEGN 283 >ref|YP_933436.1| enoyl-CoA hydratase [Azoarcus sp. BH72] emb|CAL94549.1| probable enoyl-CoA hydratase [Azoarcus sp. BH72] Length = 281 Score = 436 bits (1121), Expect = e-120, Method: Compositional matrix adjust. Identities = 204/276 (73%), Positives = 234/276 (84%) Query: 4 MKAKLRPFKDYPAKHFRWQVDASGRVATITLNRPDKKNPLTFESYEELRDLFTNLKYASD 63 M RPFKDY A+HF+W GRVATITLNRP+KKNPLTF+SY ELRDLF NLKYASD Sbjct: 6 MNDHKRPFKDYQARHFKWSCSEDGRVATITLNRPEKKNPLTFDSYAELRDLFNNLKYASD 65 Query: 64 VRAIVLTGAEGNFCSGGDVFEIIEPLTRMAMPDLLAFTRMTGEVVRAMRKCPQIIVAAID 123 VR +V+ G GNFCSGGDVFEIIEPLT+M+MP+LL FTRMTG++V+ MR+ PQ ++AAID Sbjct: 66 VRTVVIRGEGGNFCSGGDVFEIIEPLTKMSMPELLEFTRMTGDLVKTMRRAPQPVIAAID 125 Query: 124 GICAGAGAMLALASDLRLATPQAKTAFLFTRVGLAGADMGACGLLPRVIGQGHAADLLYT 183 G CAGAGAM+ALA+D RL TP+ KTA+LFTRVGLAGADMGACGLLPR+IGQG A +LL T Sbjct: 126 GFCAGAGAMVALAADFRLGTPETKTAYLFTRVGLAGADMGACGLLPRMIGQGRATELLMT 185 Query: 184 GRAMSAEEGFAWGFHNRLVPPAELAGAAQDLARSLADGPWFAHGVTKTMFNQEWAMGVDE 243 GR MSA+EG WGF + L P A+L A LAR+LADGPWFAH +TKTM NQEWAMG++E Sbjct: 186 GRVMSADEGRDWGFFSALHPSADLFAEADKLARNLADGPWFAHTMTKTMLNQEWAMGIEE 245 Query: 244 MIESEAQAQAICMVTGDFRRAFEAFAAKQKPAFEGN 279 M+ESEAQAQAICM+TGDFRRAFEAFAAKQKP FEGN Sbjct: 246 MVESEAQAQAICMMTGDFRRAFEAFAAKQKPKFEGN 281 >gb|AAL02065.1|AF320253_3 putative enoyl-CoA hydratase/isomerase [Azoarcus evansii] Length = 281 Score = 436 bits (1121), Expect = e-120, Method: Compositional matrix adjust. Identities = 209/275 (76%), Positives = 239/275 (86%) Query: 4 MKAKLRPFKDYPAKHFRWQVDASGRVATITLNRPDKKNPLTFESYEELRDLFTNLKYASD 63 M+ RPFKDY A++FRW+ GRVATITLNRP+KKNPLTF+SY ELRDLF +L YA+D Sbjct: 6 MQDHKRPFKDYAARNFRWECSDDGRVATITLNRPEKKNPLTFDSYAELRDLFRDLAYAND 65 Query: 64 VRAIVLTGAEGNFCSGGDVFEIIEPLTRMAMPDLLAFTRMTGEVVRAMRKCPQIIVAAID 123 VRA+V+ GA GNFCSGGDVFEIIEPLT+M+MP+LLAFTRMTG++V+AMR+ PQ I+AAID Sbjct: 66 VRAVVIQGAGGNFCSGGDVFEIIEPLTKMSMPELLAFTRMTGDLVKAMRRAPQPIIAAID 125 Query: 124 GICAGAGAMLALASDLRLATPQAKTAFLFTRVGLAGADMGACGLLPRVIGQGHAADLLYT 183 GICAGAGAM+ALASD RL T +AKTA+LFTRVGLAGADMGACGLLPRV+GQG A DLL T Sbjct: 126 GICAGAGAMVALASDFRLGTAEAKTAYLFTRVGLAGADMGACGLLPRVVGQGKATDLLMT 185 Query: 184 GRAMSAEEGFAWGFHNRLVPPAELAGAAQDLARSLADGPWFAHGVTKTMFNQEWAMGVDE 243 GRAM A+E A GF + + PAEL G AQ LA++LADGPWFAH +TKTM NQEWAMG++E Sbjct: 186 GRAMGADEALACGFFSAVHAPAELLGKAQALAQTLADGPWFAHTMTKTMLNQEWAMGIEE 245 Query: 244 MIESEAQAQAICMVTGDFRRAFEAFAAKQKPAFEG 278 MIESEAQAQAICMVTGDF RAFEAFAAKQKP FEG Sbjct: 246 MIESEAQAQAICMVTGDFTRAFEAFAAKQKPKFEG 280 >gb|AAL02073.1|AF320254_3 putative enoyl-CoA hydratase/isomerase [Azoarcus evansii] Length = 281 Score = 431 bits (1108), Expect = e-119, Method: Compositional matrix adjust. Identities = 205/275 (74%), Positives = 237/275 (86%) Query: 4 MKAKLRPFKDYPAKHFRWQVDASGRVATITLNRPDKKNPLTFESYEELRDLFTNLKYASD 63 M+ PFKDY A+HFRW+ GRVATITLNRP+KK+PLTF+SY ELRDLF L YASD Sbjct: 6 MQDHKHPFKDYVARHFRWECSDDGRVATITLNRPEKKDPLTFDSYAELRDLFRGLAYASD 65 Query: 64 VRAIVLTGAEGNFCSGGDVFEIIEPLTRMAMPDLLAFTRMTGEVVRAMRKCPQIIVAAID 123 VR +V+ GA GNFCSGGDVFEIIEPLT+M+MP+LLAFTRMTG++V+AMR+ PQ I+AAID Sbjct: 66 VRTVVIQGAGGNFCSGGDVFEIIEPLTKMSMPELLAFTRMTGDLVKAMRRAPQPIIAAID 125 Query: 124 GICAGAGAMLALASDLRLATPQAKTAFLFTRVGLAGADMGACGLLPRVIGQGHAADLLYT 183 GICAGAGAM+ALA+D RL T +AKTA+LFTRVGLAGADMGACGLLPRV+GQG A DLL T Sbjct: 126 GICAGAGAMVALAADFRLGTAEAKTAYLFTRVGLAGADMGACGLLPRVVGQGKATDLLMT 185 Query: 184 GRAMSAEEGFAWGFHNRLVPPAELAGAAQDLARSLADGPWFAHGVTKTMFNQEWAMGVDE 243 GRAM A+E FA GF + + PAEL G AQ LA++LADGPWFAH +TKTM NQEWAMG++E Sbjct: 186 GRAMGADEAFACGFFSAVHAPAELLGKAQTLAKTLADGPWFAHTMTKTMLNQEWAMGIEE 245 Query: 244 MIESEAQAQAICMVTGDFRRAFEAFAAKQKPAFEG 278 MIESEAQAQAICMVTGDF RAF+AFAAK+KP F+G Sbjct: 246 MIESEAQAQAICMVTGDFGRAFQAFAAKEKPKFQG 280 >ref|YP_001526104.1| enoyl-CoA hydratase [Azorhizobium caulinodans ORS 571] dbj|BAF89186.1| putative enoyl-CoA hydratase [Azorhizobium caulinodans ORS 571] Length = 283 Score = 426 bits (1094), Expect = e-117, Method: Compositional matrix adjust. Identities = 217/278 (78%), Positives = 246/278 (88%) Query: 2 SEMKAKLRPFKDYPAKHFRWQVDASGRVATITLNRPDKKNPLTFESYEELRDLFTNLKYA 61 S M A RPFKD+ A HF ++ A GRVAT+ LNRP++KNPLTFESY ELRDLF +L YA Sbjct: 6 STMAAMKRPFKDHRATHFSFETSADGRVATVRLNRPERKNPLTFESYAELRDLFRDLVYA 65 Query: 62 SDVRAIVLTGAEGNFCSGGDVFEIIEPLTRMAMPDLLAFTRMTGEVVRAMRKCPQIIVAA 121 SDVRAIVL GA GNF SGGDVFEIIEPLTRMAMPDLLAFTRMTG++V+AMR+CPQ IVAA Sbjct: 66 SDVRAIVLAGAGGNFSSGGDVFEIIEPLTRMAMPDLLAFTRMTGDLVKAMRRCPQPIVAA 125 Query: 122 IDGICAGAGAMLALASDLRLATPQAKTAFLFTRVGLAGADMGACGLLPRVIGQGHAADLL 181 +DG+CAGAGA+LA+ASDLRLATP+AKTAFLF RVGLAGADMGACG+LPRVIGQG AA+LL Sbjct: 126 VDGVCAGAGAILAMASDLRLATPEAKTAFLFARVGLAGADMGACGILPRVIGQGRAAELL 185 Query: 182 YTGRAMSAEEGFAWGFHNRLVPPAELAGAAQDLARSLADGPWFAHGVTKTMFNQEWAMGV 241 +TGRAM+A EG AWGF+N L P A++ AQ+LARSLADGPWFAHG+TKTM NQEWAMG+ Sbjct: 186 FTGRAMTAAEGAAWGFYNALHPAADMEAKAQELARSLADGPWFAHGMTKTMLNQEWAMGL 245 Query: 242 DEMIESEAQAQAICMVTGDFRRAFEAFAAKQKPAFEGN 279 +E+IE+EAQAQAICM T DFRRAFEAFAAK KPAFEGN Sbjct: 246 EELIEAEAQAQAICMATQDFRRAFEAFAAKAKPAFEGN 283