BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0227.0024.p (328 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774671.1| DNA ligase [Bradyrhizobium japonicum USDA 110] ... 456 e-126 ref|ZP_01046721.1| DNA ligase-like protein [Nitrobacter sp. Nb-3... 439 e-121 ref|YP_003019953.1| DNA ligase D [Geobacter sp. M21] >gi|2517736... 253 2e-65 ref|YP_002136955.1| DNA ligase D [Geobacter bemidjiensis Bem] >g... 253 2e-65 ref|YP_004196876.1| DNA ligase D [Geobacter sp. M18] >gi|3201240... 251 1e-64 >ref|NP_774671.1| DNA ligase [Bradyrhizobium japonicum USDA 110] dbj|BAC53296.1| blr8031 [Bradyrhizobium japonicum USDA 110] Length = 316 Score = 456 bits (1174), Expect = e-126, Method: Compositional matrix adjust. Identities = 225/279 (80%), Positives = 240/279 (86%) Query: 1 MARKSTLPYRLQPMLATLTDSPFDDPDWVFEDKFDGFRMVAEIRRGRVALYSRNGKIISH 60 M R S+LP RLQPMLATLTD PFDDP WVFEDK+DGFRM+AEIR GR ALYSRNGK+IS Sbjct: 1 MGRTSSLPKRLQPMLATLTDDPFDDPAWVFEDKYDGFRMIAEIRDGRAALYSRNGKLISR 60 Query: 61 SYVEVAKALEGVKADAVIDGELVAIGKDGASHFQLLQNALRHEAKLLYCAFDLMFADGED 120 SY EVA ALE VK DAVIDGELVAIGKDG SHFQLLQNALRHEAKLLY AFDLMFA+GED Sbjct: 61 SYTEVAAALEMVKGDAVIDGELVAIGKDGVSHFQLLQNALRHEAKLLYFAFDLMFANGED 120 Query: 121 LRAMPLLERKKRLKAILPRHKLIAFSNHRKGNGTKFFAEAERRHLEGIMAKRADSPYASG 180 LR PLLERK+RLK ILPRH LI FS HRKG G KFFAEAER LEGIMAKR D YASG Sbjct: 121 LRKRPLLERKERLKTILPRHGLIGFSRHRKGKGRKFFAEAERGGLEGIMAKRIDGAYASG 180 Query: 181 RRTADWLKVKTAQRQEVVIAGLTAPRRTRPFFGALVLAVREDDTWRYIGHVGTGFSHQVL 240 RT DWLK+KTA+RQEVVI G TAPRRTRPFFGALVLAVRE ++WRYIGHVGTGFSHQ L Sbjct: 181 SRTPDWLKIKTAKRQEVVIVGFTAPRRTRPFFGALVLAVREKESWRYIGHVGTGFSHQTL 240 Query: 241 EELHGKLVRLKSARSPFPAKVKDERVTTWVRPSLVAEIR 279 ELH +L RL+ A+SPFPAKVKDE VTTWVRP LVAE++ Sbjct: 241 AELHSRLTRLQVAKSPFPAKVKDETVTTWVRPFLVAEVK 279 >ref|ZP_01046721.1| DNA ligase-like protein [Nitrobacter sp. Nb-311A] gb|EAQ35274.1| DNA ligase-like protein [Nitrobacter sp. Nb-311A] Length = 315 Score = 439 bits (1128), Expect = e-121, Method: Compositional matrix adjust. Identities = 212/279 (75%), Positives = 242/279 (86%) Query: 1 MARKSTLPYRLQPMLATLTDSPFDDPDWVFEDKFDGFRMVAEIRRGRVALYSRNGKIISH 60 MAR STLP +LQPMLATLTD PFDD W+FEDK+DGFRMVA+I G+VALYSRNGKIISH Sbjct: 1 MARVSTLPKQLQPMLATLTDGPFDDKGWIFEDKYDGFRMVAKIASGKVALYSRNGKIISH 60 Query: 61 SYVEVAKALEGVKADAVIDGELVAIGKDGASHFQLLQNALRHEAKLLYCAFDLMFADGED 120 SY+EVA+ALEGVK DAVIDGELVA+ +G SHFQLLQNALR +A+L Y AFDLMF DG+D Sbjct: 61 SYIEVARALEGVKGDAVIDGELVALDDEGVSHFQLLQNALRRQARLRYFAFDLMFQDGDD 120 Query: 121 LRAMPLLERKKRLKAILPRHKLIAFSNHRKGNGTKFFAEAERRHLEGIMAKRADSPYASG 180 LR +PL ERKKRLKAILP+HKLIAFS RK GTKFF EAER+ LEGIMAKRADS Y SG Sbjct: 121 LRGLPLTERKKRLKAILPKHKLIAFSRDRKTFGTKFFEEAERKGLEGIMAKRADSKYQSG 180 Query: 181 RRTADWLKVKTAQRQEVVIAGLTAPRRTRPFFGALVLAVREDDTWRYIGHVGTGFSHQVL 240 RT +WLK+KT++RQEVVIAG TAPR+TRPFFGAL LA+R+ D WRYIGHVGTGFSH L Sbjct: 181 ARTDNWLKIKTSKRQEVVIAGFTAPRKTRPFFGALTLALRDGDNWRYIGHVGTGFSHDTL 240 Query: 241 EELHGKLVRLKSARSPFPAKVKDERVTTWVRPSLVAEIR 279 +ELHGKL++LK+A+SPF KVKDE VTTWV+P LVAE++ Sbjct: 241 KELHGKLIKLKTAKSPFGKKVKDEAVTTWVKPQLVAEVK 279 >ref|YP_003019953.1| DNA ligase D [Geobacter sp. M21] gb|ACT16195.1| DNA ligase D [Geobacter sp. M21] Length = 872 Score = 253 bits (647), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 130/273 (47%), Positives = 172/273 (63%), Gaps = 1/273 (0%) Query: 7 LPYRLQPMLATLTDSPFDDPDWVFEDKFDGFRMVAEIRRGRVALYSRNGKIISHSYVEVA 66 +P+RL PMLA PFDDPDW+FE K DG+R +AEI G LYSRN + + + Sbjct: 219 MPHRLTPMLARSAAEPFDDPDWLFEIKLDGYRAIAEIEDGEALLYSRNNLSFNKRFPAIV 278 Query: 67 KALEGVKADAVIDGELVAIGKDGASHFQLLQNALRH-EAKLLYCAFDLMFADGEDLRAMP 125 ++L + AV+DGE+VA+ + G S+FQLLQN R E + Y AFDL++ DGEDLRA P Sbjct: 279 QSLASLPVTAVLDGEVVALDEKGRSYFQLLQNNRRTGEGNIYYYAFDLLYVDGEDLRAEP 338 Query: 126 LLERKKRLKAILPRHKLIAFSNHRKGNGTKFFAEAERRHLEGIMAKRADSPYASGRRTAD 185 L RK+RL+++LP + F +H + G +FF A +LEGI+AKRADS Y +GRR+ D Sbjct: 339 LEARKERLRSLLPELPWVRFGDHIREYGRQFFELARENNLEGILAKRADSRYLAGRRSGD 398 Query: 186 WLKVKTAQRQEVVIAGLTAPRRTRPFFGALVLAVREDDTWRYIGHVGTGFSHQVLEELHG 245 WLK+K +QE VI G T PR +R G+LVL V E+D YIG G GF L+E++ Sbjct: 399 WLKIKIRLQQEAVICGFTQPRGSRKGLGSLVLGVYENDELVYIGLTGGGFDDAGLKEMYA 458 Query: 246 KLVRLKSARSPFPAKVKDERVTTWVRPSLVAEI 278 L L SPF +VK TWV+P LV E+ Sbjct: 459 ALQLLVQPESPFRQQVKTSMPATWVKPVLVCEV 491 >ref|YP_002136955.1| DNA ligase D [Geobacter bemidjiensis Bem] gb|ACH37159.1| DNA ligase D, ATP-dependent [Geobacter bemidjiensis Bem] Length = 871 Score = 253 bits (647), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 131/275 (47%), Positives = 172/275 (62%), Gaps = 1/275 (0%) Query: 5 STLPYRLQPMLATLTDSPFDDPDWVFEDKFDGFRMVAEIRRGRVALYSRNGKIISHSYVE 64 + +P+RL PMLA PFDDPDW+FE K DG+R +AEI G V LYSRN + + Sbjct: 215 APMPHRLTPMLANSAAEPFDDPDWLFEIKLDGYRAIAEIEGGEVQLYSRNNLSFNKRFPA 274 Query: 65 VAKALEGVKADAVIDGELVAIGKDGASHFQLLQNALRH-EAKLLYCAFDLMFADGEDLRA 123 + ++L + AV+DGE+VA+ G S FQLLQN+LR E + Y AFDL++ DGEDLRA Sbjct: 275 IVQSLASLPVTAVLDGEVVALDAKGRSSFQLLQNSLRTGEKNIYYYAFDLLYLDGEDLRA 334 Query: 124 MPLLERKKRLKAILPRHKLIAFSNHRKGNGTKFFAEAERRHLEGIMAKRADSPYASGRRT 183 PL RK+RL+ +LP + FS+H + G +FF A +LEGI+AKRADS Y +GRR+ Sbjct: 335 EPLQARKERLRNLLPELPWVRFSDHIREYGRQFFELARENNLEGILAKRADSRYFAGRRS 394 Query: 184 ADWLKVKTAQRQEVVIAGLTAPRRTRPFFGALVLAVREDDTWRYIGHVGTGFSHQVLEEL 243 DWLK+K +QE VI G T PR +R G+LVL V E+ YIG G GF L+E+ Sbjct: 395 GDWLKIKIRLQQEAVICGFTQPRGSRKGLGSLVLGVYENGELVYIGLTGGGFDDVGLKEM 454 Query: 244 HGKLVRLKSARSPFPAKVKDERVTTWVRPSLVAEI 278 + L L SPF +VK TW +P LV E+ Sbjct: 455 YAALQPLIQPESPFRQQVKTSMPVTWTKPVLVCEV 489 >ref|YP_004196876.1| DNA ligase D [Geobacter sp. M18] gb|ADW11600.1| DNA ligase D [Geobacter sp. M18] Length = 892 Score = 251 bits (640), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 129/276 (46%), Positives = 174/276 (63%), Gaps = 1/276 (0%) Query: 4 KSTLPYRLQPMLATLTDSPFDDPDWVFEDKFDGFRMVAEIRRGRVALYSRNGKIISHSYV 63 ++ +P R+ PMLAT T PFDDPDW+FE K DG+R +AE+ VALYSRN + + Sbjct: 239 EAPMPRRVSPMLATSTAEPFDDPDWLFEIKLDGYRAIAEVSGREVALYSRNNLSFNKKFP 298 Query: 64 EVAKALEGVKADAVIDGELVAIGKDGASHFQLLQNALRH-EAKLLYCAFDLMFADGEDLR 122 + +L + +AV DGE+VA+ ++G S+FQLLQN R + + Y FDL++ +GEDLR Sbjct: 299 SLPGSLASLDVEAVFDGEVVALDENGRSYFQLLQNYRRTGKGNIAYFIFDLLYLNGEDLR 358 Query: 123 AMPLLERKKRLKAILPRHKLIAFSNHRKGNGTKFFAEAERRHLEGIMAKRADSPYASGRR 182 PLL RK+RL+ +LP + +S+H G +FF A + +LEGI+AKRA S Y +GRR Sbjct: 359 NQPLLARKERLRTLLPDLPELRYSDHIMEYGREFFELARQNNLEGILAKRAASLYQTGRR 418 Query: 183 TADWLKVKTAQRQEVVIAGLTAPRRTRPFFGALVLAVREDDTWRYIGHVGTGFSHQVLEE 242 + DWLK+K +QE VI G T PRR+R FGALVL V EDD IG G GF L+E Sbjct: 419 SKDWLKIKIRMQQEAVICGFTQPRRSRKGFGALVLGVYEDDRLVCIGFAGGGFDEAGLKE 478 Query: 243 LHGKLVRLKSARSPFPAKVKDERVTTWVRPSLVAEI 278 ++ L L PF VK + TWV+P LV E+ Sbjct: 479 MYELLQPLVQPEPPFGTPVKSDMPITWVKPELVCEV 514