BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0004.p (295 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004153103.1| NAD-dependent epimerase/dehydratase [Variovo... 410 e-113 ref|YP_002499499.1| NAD-dependent epimerase/dehydratase [Methylo... 343 1e-92 ref|YP_004370187.1| dTDP-glucose 4,6-dehydratase [Desulfobacca a... 333 2e-89 ref|YP_566237.1| NAD-dependent epimerase/dehydratase [Methanococ... 328 5e-88 ref|YP_460444.1| dTDP-glucose 4,6-dehydratase [Syntrophus acidit... 318 7e-85 >ref|YP_004153103.1| NAD-dependent epimerase/dehydratase [Variovorax paradoxus EPS] gb|ADU34992.1| NAD-dependent epimerase/dehydratase [Variovorax paradoxus EPS] Length = 334 Score = 410 bits (1055), Expect = e-113, Method: Compositional matrix adjust. Identities = 199/295 (67%), Positives = 235/295 (79%) Query: 1 MIRYSSSPAWGSLEHLSPDLKNSINVVAGNIEDPEFVTQTVRDQEIVFHLAALIAIPYSY 60 M+RY S+ +WG+LE D+K VVAGNI+D +FV ++ ++V HLAALIAIPYSY Sbjct: 40 MVRYGSASSWGNLEFAPDDVKGQFRVVAGNIDDGDFVMHAMQGIDVVCHLAALIAIPYSY 99 Query: 61 VAPRSYVRTNIEGTLNVVEAARQLGVSRVVHTSTSEVYGTAITETIDEAHPLQGQSPYSA 120 VAPRSYVRTN+EGTLNVVEAAR+L V RVVHTSTSEVYGTA+ IDE HPLQGQSPYSA Sbjct: 100 VAPRSYVRTNVEGTLNVVEAARRLSVRRVVHTSTSEVYGTAVRVPIDEEHPLQGQSPYSA 159 Query: 121 SKIGADKIAESYSRSFSTPAVTVRPFNTFGPRQSARAFIPTIISQALTRDVIELGALDTL 180 SKIGADKIAESY RSF T VT+RPFNTFGPRQSARAFIPTIISQALT+DVI+LG+L Sbjct: 160 SKIGADKIAESYYRSFDTGVVTLRPFNTFGPRQSARAFIPTIISQALTQDVIKLGSLTPE 219 Query: 181 RDMTFVGDTVSGFLAAGATPGIEGETINLGTGQTFPVSHFAERILSLMNVRKQIVQDPKR 240 RDMTFV DTV+GF+AA TPGIEG+TINLGTG T+ + HFAERIL LM V K+IV D R Sbjct: 220 RDMTFVTDTVAGFVAASVTPGIEGQTINLGTGSTYTIGHFAERILKLMGVEKEIVADTAR 279 Query: 241 IRPAMSEVLRLVSNNGKAQQLLQWTPGTSIDEGLKLTINFVEANIGSYRANLYNI 295 +RP SEVL+LVS+N A +L+ W+P +DEGL+ +I F+ NI YR +Y + Sbjct: 280 MRPDKSEVLKLVSDNALAAKLMGWSPQVDLDEGLRQSIEFIRRNIHLYRPAVYTV 334 >ref|YP_002499499.1| NAD-dependent epimerase/dehydratase [Methylobacterium nodulans ORS 2060] gb|ACL59196.1| NAD-dependent epimerase/dehydratase [Methylobacterium nodulans ORS 2060] Length = 327 Score = 343 bits (881), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 176/293 (60%), Positives = 206/293 (70%) Query: 1 MIRYSSSPAWGSLEHLSPDLKNSINVVAGNIEDPEFVTQTVRDQEIVFHLAALIAIPYSY 60 ++ Y+++ +L PDL + +V G++ DPEFV V QEIV HLAALIAIPYSY Sbjct: 33 LLHYNAARDLANLADADPDLTAGMRIVFGDVNDPEFVLSLVEGQEIVLHLAALIAIPYSY 92 Query: 61 VAPRSYVRTNIEGTLNVVEAARQLGVSRVVHTSTSEVYGTAITETIDEAHPLQGQSPYSA 120 APRSYVRTNIEGTLNV+EAAR++GV RVVHTSTSEVYGTA IDE HPLQGQSPYSA Sbjct: 93 TAPRSYVRTNIEGTLNVLEAARRVGVERVVHTSTSEVYGTAQYVPIDEVHPLQGQSPYSA 152 Query: 121 SKIGADKIAESYSRSFSTPAVTVRPFNTFGPRQSARAFIPTIISQALTRDVIELGALDTL 180 SKIGADK+AESY RSF P VTVRPFNT+GPRQSARAFIPT+I QAL I+LGA + Sbjct: 153 SKIGADKVAESYYRSFDVPVVTVRPFNTYGPRQSARAFIPTVIGQALRGGEIKLGATSPV 212 Query: 181 RDMTFVGDTVSGFLAAGATPGIEGETINLGTGQTFPVSHFAERILSLMNVRKQIVQDPKR 240 RD+TFV DT +GFLAA TPG+EG T NLG G V AE IL LM +IV D +R Sbjct: 213 RDLTFVADTAAGFLAAATTPGLEGGTYNLGVGSGQSVGEVAEMILRLMGSSARIVTDIQR 272 Query: 241 IRPAMSEVLRLVSNNGKAQQLLQWTPGTSIDEGLKLTINFVEANIGSYRANLY 293 +RP SEV RLVS+N + W P ++EGL TI+F N A+ Y Sbjct: 273 LRPEKSEVGRLVSDNRRFFAASGWAPAIKLEEGLARTIDFFRRNPNRRDADTY 325 >ref|YP_004370187.1| dTDP-glucose 4,6-dehydratase [Desulfobacca acetoxidans DSM 11109] gb|AEB09006.1| dTDP-glucose 4,6-dehydratase [Desulfobacca acetoxidans DSM 11109] Length = 335 Score = 333 bits (853), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 160/295 (54%), Positives = 209/295 (70%) Query: 1 MIRYSSSPAWGSLEHLSPDLKNSINVVAGNIEDPEFVTQTVRDQEIVFHLAALIAIPYSY 60 ++ Y+S WG LE + PDL+ + V+AG+I DP + + D EIVFHLAALIAIPYSY Sbjct: 41 LVHYNSRSDWGLLELIHPDLRGQLEVMAGDIIDPFCAFRAMADCEIVFHLAALIAIPYSY 100 Query: 61 VAPRSYVRTNIEGTLNVVEAARQLGVSRVVHTSTSEVYGTAITETIDEAHPLQGQSPYSA 120 +AP +YV N GTLN++EAARQ V R VHTSTSE YGTA IDE HPL+GQSPYSA Sbjct: 101 IAPANYVTVNCGGTLNLLEAARQHRVRRFVHTSTSETYGTARYTPIDEEHPLKGQSPYSA 160 Query: 121 SKIGADKIAESYSRSFSTPAVTVRPFNTFGPRQSARAFIPTIISQALTRDVIELGALDTL 180 SKIGADK+AESY SF TP T+RPFNT+GPRQSARA IPTIISQ L+ ++I LG+L L Sbjct: 161 SKIGADKLAESYFLSFGTPVSTIRPFNTYGPRQSARAVIPTIISQGLSGNIIRLGSLTPL 220 Query: 181 RDMTFVGDTVSGFLAAGATPGIEGETINLGTGQTFPVSHFAERILSLMNVRKQIVQDPKR 240 RD+ FV DTV+GFL + G+ IN+G+G++ + A I++L+ K+I+ + +R Sbjct: 221 RDLNFVSDTVAGFLKVAESDAAVGQVINVGSGKSVSIGQLAYMIITLLGGNKEIICEDQR 280 Query: 241 IRPAMSEVLRLVSNNGKAQQLLQWTPGTSIDEGLKLTINFVEANIGSYRANLYNI 295 +RP SEV+ L+ + KA+ LL W P S++EGL TI F+ N+G Y+ LYN+ Sbjct: 281 VRPEASEVMELLCDYSKAKALLDWEPMISLEEGLSHTIAFIRENLGQYKPYLYNV 335 >ref|YP_566237.1| NAD-dependent epimerase/dehydratase [Methanococcoides burtonii DSM 6242] gb|ABE52487.1| nucleotide sugar dehydratase [Methanococcoides burtonii DSM 6242] Length = 330 Score = 328 bits (842), Expect = 5e-88, Method: Compositional matrix adjust. Identities = 158/296 (53%), Positives = 206/296 (69%), Gaps = 1/296 (0%) Query: 1 MIRYSSSPAWGSLEHLSPDLKNSINVVAGNIEDPEFVTQTVRDQEIVFHLAALIAIPYSY 60 + Y+S WG LE DL + + V++G++ DP FV +D +IVFHLAALI IPYSY Sbjct: 35 FVHYNSRNDWGLLELAEKDLLDKVEVISGDVTDPFFVNSVTKDTDIVFHLAALIGIPYSY 94 Query: 61 VAPRSYVRTNIEGTLNVVEAARQLGVSRVVHTSTSEVYGTAITETIDEAHPLQGQSPYSA 120 VAP YV N++GTLNV++A GV ++VHTSTSE YGTA IDE HPL+GQSPYSA Sbjct: 95 VAPEHYVNVNVKGTLNVLQACLDNGVGKMVHTSTSETYGTAEYTPIDEKHPLKGQSPYSA 154 Query: 121 SKIGADKIAESYSRSFSTPAVTVRPFNTFGPRQSARAFIPTIISQALTRDVIELGALDTL 180 SKIGADK+AESY RSF P T+RPFNTFGPRQSARA IPTIISQALTRD I +G+L + Sbjct: 155 SKIGADKMAESYYRSFDLPVATIRPFNTFGPRQSARAIIPTIISQALTRDEIHVGSLTPV 214 Query: 181 RDMTFVGDTVSGFLAAGATPGIEGETINLGTGQTFPVSHFAERILSLMNVRK-QIVQDPK 239 RDMT+V DTV+GF+ G + GETIN+GTG + E+IL ++ ++++ Sbjct: 215 RDMTYVKDTVNGFIEVGLSERSSGETINVGTGSGATIGVILEKILKILGKEDIPVIEEQN 274 Query: 240 RIRPAMSEVLRLVSNNGKAQQLLQWTPGTSIDEGLKLTINFVEANIGSYRANLYNI 295 R+RP SEV+ L+ +N KA+ LL W+P S+D GLK TI +++ Y+ ++Y I Sbjct: 275 RVRPGKSEVMELICDNSKAKDLLNWSPEYSLDNGLKETIEWMQEYTKLYKPDMYVI 330 >ref|YP_460444.1| dTDP-glucose 4,6-dehydratase [Syntrophus aciditrophicus SB] gb|ABC76276.1| dTDP-glucose 4,6-dehydratase [Syntrophus aciditrophicus SB] Length = 331 Score = 318 bits (814), Expect = 7e-85, Method: Compositional matrix adjust. Identities = 159/295 (53%), Positives = 200/295 (67%) Query: 1 MIRYSSSPAWGSLEHLSPDLKNSINVVAGNIEDPEFVTQTVRDQEIVFHLAALIAIPYSY 60 +RY+S G L+ P++ + I ++ G++ D + V + V IVFHL ALI+IPYSY Sbjct: 37 FVRYNSRADAGLLKLAPPEILSRIELIGGDLRDSDAVRKAVEGCRIVFHLGALISIPYSY 96 Query: 61 VAPRSYVRTNIEGTLNVVEAARQLGVSRVVHTSTSEVYGTAITETIDEAHPLQGQSPYSA 120 P TN+ GTLNV+ A R LGV R++HTSTSEVYGTA IDEAHPLQGQSPYSA Sbjct: 97 YHPVEVAETNLMGTLNVLLACRDLGVERLIHTSTSEVYGTAQRVPIDEAHPLQGQSPYSA 156 Query: 121 SKIGADKIAESYSRSFSTPAVTVRPFNTFGPRQSARAFIPTIISQALTRDVIELGALDTL 180 SKIGADK+AES+ +FS P VTVRPFNTFGPRQSARA IPTII+Q L R I LG L+T Sbjct: 157 SKIGADKLAESFFCAFSVPVVTVRPFNTFGPRQSARAVIPTIITQVLARQSIRLGNLETT 216 Query: 181 RDMTFVGDTVSGFLAAGATPGIEGETINLGTGQTFPVSHFAERILSLMNVRKQIVQDPKR 240 RD T+V DTVSGFL A GIEG T NLGTG + A I+ + V + DP+R Sbjct: 217 RDFTYVDDTVSGFLCAAQKEGIEGMTFNLGTGSEIAIGVLARTIIEKIGVPVSVELDPER 276 Query: 241 IRPAMSEVLRLVSNNGKAQQLLQWTPGTSIDEGLKLTINFVEANIGSYRANLYNI 295 +RP SEV+RL+S+N +A+ L W P +DEGL TI ++ N+G+YR +Y + Sbjct: 277 LRPEGSEVMRLLSDNSRARSQLGWGPEVGLDEGLNRTIAWIRENLGNYRIGVYEL 331