BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0224.0038.p (518 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAL35755.1| proline dehydrogenase [Bradyrhizobium japonicum] 752 0.0 ref|NP_773901.1| bifunctional proline dehydrogenase/pyrroline-5-... 744 0.0 pdb|3HAZ|A Chain A, Crystal Structure Of Bifunctional Proline Ut... 744 0.0 emb|CAA62038.1| proline dehydrogenase [Bradyrhizobium japonicum] 741 0.0 ref|ZP_01044881.1| delta-1-pyrroline-5-carboxylate dehydrogenase... 734 0.0 >gb|AAL35755.1| proline dehydrogenase [Bradyrhizobium japonicum] Length = 1017 Score = 752 bits (1942), Expect = 0.0, Method: Compositional matrix adjust. Identities = 373/454 (82%), Positives = 404/454 (88%), Gaps = 3/454 (0%) Query: 1 MPDPSPLPSPFSAPYAPDDAAIAARLRQDAKLGAEAEARIDRTATRLIEAIRANDDPLGG 60 MP+ +P PF+APYAPDDA IAARL + L EARI RTATRLIEAIR DD LGG Sbjct: 1 MPN---IPPPFTAPYAPDDAEIAARLVPASHLSPPQEARIHRTATRLIEAIRKRDDRLGG 57 Query: 61 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGQGDFVNHETRSSAFLVN 120 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLG+GDF++HET+S+AFLVN Sbjct: 58 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVN 117 Query: 121 ASAWALGMSARVIQPGETPQGTIGRLTKRLGAPAVRAATRQAMRLMGSHFVLGETIEAAL 180 ASAWALG+SARVIQPGETP GTIGRL KRLGAPAVR ATRQAMRLMG+HFVLGETIE AL Sbjct: 118 ASAWALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQAMRLMGNHFVLGETIEQAL 177 Query: 181 SRAQSHTARGSRYSFDMLGEGARTADDAHRYFESYARAIEAIGKAAGDQPLPDRPGISVK 240 R + + + +RYSFDMLGEGARTA DA RYF++YA AIE IGKAAG+ PLPDRPGISVK Sbjct: 178 ERGKPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHPLPDRPGISVK 237 Query: 241 LSALHPRFEAVSHARVMTELVPQLIDLARRAKAFDLNFTVDAEEADRLELSLDVIAAAFA 300 LSALHPRFEA+S ARVM+ELVPQL+DLA+RAKA DLNFTVDAEEADRLELSLDVIAA A Sbjct: 238 LSALHPRFEAISRARVMSELVPQLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLA 297 Query: 301 DPSLAGWSGFGLAIQAYQKRAADVIDYVDALTRALDRSMMVRLVKGAYWDTEIKRAQERG 360 DPSL GW GFGLAIQAYQKRA+ VIDYVDAL RA DR +MVRLVKGAYWDTEIKRAQERG Sbjct: 298 DPSLNGWDGFGLAIQAYQKRASAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERG 357 Query: 361 LDGYPVFTRKAMTDLNYVACARKLLALRPRLFPQFATHNALTVATILDLADDPSSFEFQR 420 LDGYPVFTRKAMTDLNYVACA KLL LRPR+FPQFATHNALTVAT+L++A D SFEFQR Sbjct: 358 LDGYPVFTRKAMTDLNYVACASKLLTLRPRIFPQFATHNALTVATVLEMAQDSGSFEFQR 417 Query: 421 LHGMGEALYAQLGEDRPEIAHRTYAPVGSHRDLL 454 LHGMGEALY QL +D P+IA+RTYAPVGSHRDLL Sbjct: 418 LHGMGEALYEQLAKDHPDIAYRTYAPVGSHRDLL 451 >ref|NP_773901.1| bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase [Bradyrhizobium japonicum USDA 110] dbj|BAC52526.1| proline dehydrogenase [Bradyrhizobium japonicum USDA 110] Length = 999 Score = 744 bits (1921), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/454 (81%), Positives = 402/454 (88%), Gaps = 3/454 (0%) Query: 1 MPDPSPLPSPFSAPYAPDDAAIAARLRQDAKLGAEAEARIDRTATRLIEAIRANDDPLGG 60 MP+ +P PF+APYAPDDA IAARL + L EARI RTATRLIEAIR DD LGG Sbjct: 1 MPN---IPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKRDDRLGG 57 Query: 61 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGQGDFVNHETRSSAFLVN 120 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLG+GDF++HET+S+AFLVN Sbjct: 58 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVN 117 Query: 121 ASAWALGMSARVIQPGETPQGTIGRLTKRLGAPAVRAATRQAMRLMGSHFVLGETIEAAL 180 ASAWALG+SARVIQPGETP GTIGRL KRLGAPAVR ATRQAMRLMG+HFVLGETIE AL Sbjct: 118 ASAWALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQAMRLMGNHFVLGETIEQAL 177 Query: 181 SRAQSHTARGSRYSFDMLGEGARTADDAHRYFESYARAIEAIGKAAGDQPLPDRPGISVK 240 R + + + +RYSFDMLGEGARTA DA RYF++YA AIE IGKAAG+ LPDRPGISVK Sbjct: 178 ERGKPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHALPDRPGISVK 237 Query: 241 LSALHPRFEAVSHARVMTELVPQLIDLARRAKAFDLNFTVDAEEADRLELSLDVIAAAFA 300 LSALHPRFEA+S ARVM ELVPQL+DLA+RAKA DLNFTVDAEEADRLELSLDVIAA A Sbjct: 238 LSALHPRFEAISRARVMVELVPQLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLA 297 Query: 301 DPSLAGWSGFGLAIQAYQKRAADVIDYVDALTRALDRSMMVRLVKGAYWDTEIKRAQERG 360 DPSL GW GFGLAIQAYQKRA+ VIDYVDAL RA DR +MVRLVKGAYWDTEIKRAQERG Sbjct: 298 DPSLKGWDGFGLAIQAYQKRASAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERG 357 Query: 361 LDGYPVFTRKAMTDLNYVACARKLLALRPRLFPQFATHNALTVATILDLADDPSSFEFQR 420 LDGYPVFTRKAMTDLNYVACA KLLALRPR+FPQFATHNALTVAT+L++A+ S FEFQR Sbjct: 358 LDGYPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNALTVATVLEMAEGSSGFEFQR 417 Query: 421 LHGMGEALYAQLGEDRPEIAHRTYAPVGSHRDLL 454 LHGMGEALY QL +D +IA+RTYAPVGSHRDLL Sbjct: 418 LHGMGEALYEQLAKDHADIAYRTYAPVGSHRDLL 451 >pdb|3HAZ|A Chain A, Crystal Structure Of Bifunctional Proline Utilization A (Puta) Protein pdb|3HAZ|B Chain B, Crystal Structure Of Bifunctional Proline Utilization A (Puta) Protein Length = 1001 Score = 744 bits (1921), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/454 (81%), Positives = 402/454 (88%), Gaps = 3/454 (0%) Query: 1 MPDPSPLPSPFSAPYAPDDAAIAARLRQDAKLGAEAEARIDRTATRLIEAIRANDDPLGG 60 MP+ +P PF+APYAPDDA IAARL + L EARI RTATRLIEAIR DD LGG Sbjct: 3 MPN---IPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKRDDRLGG 59 Query: 61 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGQGDFVNHETRSSAFLVN 120 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLG+GDF++HET+S+AFLVN Sbjct: 60 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVN 119 Query: 121 ASAWALGMSARVIQPGETPQGTIGRLTKRLGAPAVRAATRQAMRLMGSHFVLGETIEAAL 180 ASAWALG+SARVIQPGETP GTIGRL KRLGAPAVR ATRQAMRLMG+HFVLGETIE AL Sbjct: 120 ASAWALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQAMRLMGNHFVLGETIEQAL 179 Query: 181 SRAQSHTARGSRYSFDMLGEGARTADDAHRYFESYARAIEAIGKAAGDQPLPDRPGISVK 240 R + + + +RYSFDMLGEGARTA DA RYF++YA AIE IGKAAG+ LPDRPGISVK Sbjct: 180 ERGKPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHALPDRPGISVK 239 Query: 241 LSALHPRFEAVSHARVMTELVPQLIDLARRAKAFDLNFTVDAEEADRLELSLDVIAAAFA 300 LSALHPRFEA+S ARVM ELVPQL+DLA+RAKA DLNFTVDAEEADRLELSLDVIAA A Sbjct: 240 LSALHPRFEAISRARVMVELVPQLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLA 299 Query: 301 DPSLAGWSGFGLAIQAYQKRAADVIDYVDALTRALDRSMMVRLVKGAYWDTEIKRAQERG 360 DPSL GW GFGLAIQAYQKRA+ VIDYVDAL RA DR +MVRLVKGAYWDTEIKRAQERG Sbjct: 300 DPSLKGWDGFGLAIQAYQKRASAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERG 359 Query: 361 LDGYPVFTRKAMTDLNYVACARKLLALRPRLFPQFATHNALTVATILDLADDPSSFEFQR 420 LDGYPVFTRKAMTDLNYVACA KLLALRPR+FPQFATHNALTVAT+L++A+ S FEFQR Sbjct: 360 LDGYPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNALTVATVLEMAEGSSGFEFQR 419 Query: 421 LHGMGEALYAQLGEDRPEIAHRTYAPVGSHRDLL 454 LHGMGEALY QL +D +IA+RTYAPVGSHRDLL Sbjct: 420 LHGMGEALYEQLAKDHADIAYRTYAPVGSHRDLL 453 >emb|CAA62038.1| proline dehydrogenase [Bradyrhizobium japonicum] Length = 1016 Score = 741 bits (1912), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/454 (81%), Positives = 401/454 (88%), Gaps = 3/454 (0%) Query: 1 MPDPSPLPSPFSAPYAPDDAAIAARLRQDAKLGAEAEARIDRTATRLIEAIRANDDPLGG 60 MP+ +P PF+APYAPDDA IAARL + L EARI RTATRLIEAIR DD LGG Sbjct: 1 MPN---IPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKRDDRLGG 57 Query: 61 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGQGDFVNHETRSSAFLVN 120 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLG+GDF++HET+S+AFLVN Sbjct: 58 VEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVN 117 Query: 121 ASAWALGMSARVIQPGETPQGTIGRLTKRLGAPAVRAATRQAMRLMGSHFVLGETIEAAL 180 ASAWALG+SARVIQPGETP GTIGRL KRLGAPAVR ATRQAMRLMG+HFVLGETIE AL Sbjct: 118 ASAWALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQAMRLMGNHFVLGETIEQAL 177 Query: 181 SRAQSHTARGSRYSFDMLGEGARTADDAHRYFESYARAIEAIGKAAGDQPLPDRPGISVK 240 R + + + +RYSFDMLGEGARTA DA RYF++YA AIE IGKAAG+ LPDRPGISVK Sbjct: 178 ERGKPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHALPDRPGISVK 237 Query: 241 LSALHPRFEAVSHARVMTELVPQLIDLARRAKAFDLNFTVDAEEADRLELSLDVIAAAFA 300 LSALHPRFEA+S ARVM ELVPQL+DLA+R KA DLNFTVDAEEADRLELSLDVIAA A Sbjct: 238 LSALHPRFEAISRARVMVELVPQLLDLAQRGKAHDLNFTVDAEEADRLELSLDVIAATLA 297 Query: 301 DPSLAGWSGFGLAIQAYQKRAADVIDYVDALTRALDRSMMVRLVKGAYWDTEIKRAQERG 360 DPSL GW GFGLAIQAYQKRA+ VIDYVDAL RA DR +MVRLVKGAYWDTEIKRAQERG Sbjct: 298 DPSLKGWDGFGLAIQAYQKRASAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERG 357 Query: 361 LDGYPVFTRKAMTDLNYVACARKLLALRPRLFPQFATHNALTVATILDLADDPSSFEFQR 420 LDGYPVFTRKAMTDLNYVACA KLLALRPR+FPQFATHNALTVAT+L++A+ S FEFQR Sbjct: 358 LDGYPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNALTVATVLEMAEGSSGFEFQR 417 Query: 421 LHGMGEALYAQLGEDRPEIAHRTYAPVGSHRDLL 454 LHGMGEALY QL +D +IA+RTYAPVGSHRDLL Sbjct: 418 LHGMGEALYEQLAKDHADIAYRTYAPVGSHRDLL 451 >ref|ZP_01044881.1| delta-1-pyrroline-5-carboxylate dehydrogenase 3 [Nitrobacter sp. Nb-311A] gb|EAQ36886.1| delta-1-pyrroline-5-carboxylate dehydrogenase 3 [Nitrobacter sp. Nb-311A] Length = 1002 Score = 734 bits (1896), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/451 (80%), Positives = 397/451 (88%) Query: 4 PSPLPSPFSAPYAPDDAAIAARLRQDAKLGAEAEARIDRTATRLIEAIRANDDPLGGVED 63 P LP PFS PYAPDD IA +L +A+L E E RIDRTA RLI++IR NDDPLGGVED Sbjct: 2 PPKLPPPFSVPYAPDDRPIAEQLLAEARLTPERETRIDRTARRLIDSIRQNDDPLGGVED 61 Query: 64 MLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGQGDFVNHETRSSAFLVNASA 123 MLREFALSTKEGLALMVLAEALLRVPDA TAD+FIED+LGQGDF++HETRSSAFLVNASA Sbjct: 62 MLREFALSTKEGLALMVLAEALLRVPDAETADRFIEDRLGQGDFIHHETRSSAFLVNASA 121 Query: 124 WALGMSARVIQPGETPQGTIGRLTKRLGAPAVRAATRQAMRLMGSHFVLGETIEAALSRA 183 WALG+S RVIQPGETPQGTIGRL KRLG PA+RAATRQAMRLMGSHFVLGETIEAAL RA Sbjct: 122 WALGLSTRVIQPGETPQGTIGRLAKRLGVPAIRAATRQAMRLMGSHFVLGETIEAALERA 181 Query: 184 QSHTARGSRYSFDMLGEGARTADDAHRYFESYARAIEAIGKAAGDQPLPDRPGISVKLSA 243 +H+A +RYSFDMLGEGARTA DA RYF SYA AI+AIG++A D PLP+RPGISVKLSA Sbjct: 182 HAHSAGCARYSFDMLGEGARTASDADRYFNSYAAAIKAIGRSAADNPLPNRPGISVKLSA 241 Query: 244 LHPRFEAVSHARVMTELVPQLIDLARRAKAFDLNFTVDAEEADRLELSLDVIAAAFADPS 303 LHPRFEAVS ARVMTELVP+ IDLARRAKAFDL FT+DAEEADRLELSLDVIAA AD S Sbjct: 242 LHPRFEAVSRARVMTELVPRAIDLARRAKAFDLAFTIDAEEADRLELSLDVIAAVAADRS 301 Query: 304 LAGWSGFGLAIQAYQKRAADVIDYVDALTRALDRSMMVRLVKGAYWDTEIKRAQERGLDG 363 L GWSGFGLA+QAYQKRA VIDY+D L RAL+R M VRLVKGAYWDTEIKRAQERG++G Sbjct: 302 LGGWSGFGLAVQAYQKRAGAVIDYIDDLARALNRRMTVRLVKGAYWDTEIKRAQERGING 361 Query: 364 YPVFTRKAMTDLNYVACARKLLALRPRLFPQFATHNALTVATILDLADDPSSFEFQRLHG 423 YPVFTRKAMTDLNY+ACA+KLLALRPRLFPQFATHNALTVAT+L+L + FEFQRLHG Sbjct: 362 YPVFTRKAMTDLNYLACAKKLLALRPRLFPQFATHNALTVATLLELTGGETGFEFQRLHG 421 Query: 424 MGEALYAQLGEDRPEIAHRTYAPVGSHRDLL 454 MGEALY L ++ P IA R YAPVGSHRDLL Sbjct: 422 MGEALYTTLCKEHPAIACRIYAPVGSHRDLL 452