BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0224.0012.p (550 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_764782.1| acetolactate synthase [Rhizobium leguminosarum ... 879 0.0 ref|NP_770663.1| acetolactate synthase [Bradyrhizobium japonicum... 872 0.0 ref|YP_001755075.1| thiamine pyrophosphate binding domain-contai... 858 0.0 ref|YP_002973558.1| acetolactate synthase [Rhizobium leguminosar... 856 0.0 ref|YP_004144397.1| thiamine pyrophosphate TPP-binding domain-co... 855 0.0 >ref|YP_764782.1| acetolactate synthase [Rhizobium leguminosarum bv. viciae 3841] emb|CAK11983.1| putative acetolactate synthase [Rhizobium leguminosarum bv. viciae 3841] Length = 548 Score = 879 bits (2270), Expect = 0.0, Method: Compositional matrix adjust. Identities = 416/547 (76%), Positives = 483/547 (88%), Gaps = 2/547 (0%) Query: 1 MVKGSDLLVAALENEGVERVFGVPGEENLDVVESLRKSSIKLIVTRHEQAAAFMAATYGR 60 M KGSDLLVAA+ENEGV+R+FG+PGEENLDVVES+RKSSI+L++TRHEQAAAFMAATYGR Sbjct: 1 MTKGSDLLVAAIENEGVDRIFGIPGEENLDVVESIRKSSIELVLTRHEQAAAFMAATYGR 60 Query: 61 LTGKPGVCMTTLGPGALNLTTGAAYALLGAMPMVMLTGQKGILSSRQAKFQIVDIVNTFR 120 LTGKPGVC+TTLGPGALNL+TGAAYALLGAMPMVM+TGQKGILSSRQA+FQ+VD+V + + Sbjct: 61 LTGKPGVCLTTLGPGALNLSTGAAYALLGAMPMVMITGQKGILSSRQARFQVVDVVASMK 120 Query: 121 PLTKLSLQIVSGATIPTLVRDAFRIATQERPGPVLLELPEDIAGEEIAPVEMISPHPIEI 180 PLTKL+ QIVS IPT VR+AFRIA +E+PGPV LELPEDIA EE V +I+PH +E+ Sbjct: 121 PLTKLARQIVSPQMIPTTVREAFRIAQEEKPGPVHLELPEDIAAEECQEVALIAPHQLEL 180 Query: 181 PIAHAAALDRAADMILKAQRPLIMLGAAASRPRSTAGIGDFVQRTKIPFFTTQMGKGTVS 240 P A AALDRAAD+I A+RPL+M GAAASRPRST+ I FV RT+IPFFTTQMGKGTV Sbjct: 181 PTASDAALDRAADLIAAAKRPLLMFGAAASRPRSTSDIAQFVIRTRIPFFTTQMGKGTVP 240 Query: 241 GGTNQYMGTAALSERDYVHEAIDRADLIIAIGHDTVEKPPFIMGPKGPQVIHVSYMPATV 300 GGT YMGTAALSERDYVHEAI++ADLII IGHDT+EKPPFIMG GP+V+HV Y PATV Sbjct: 241 GGTELYMGTAALSERDYVHEAIEQADLIITIGHDTIEKPPFIMGKGGPKVVHVGYQPATV 300 Query: 301 EQVYFPQCEVIGDVGPSLELLADRVEGKLPHASALLSLRQGILAKITDRATEGRWPPTPQ 360 EQVYFPQ EVIGD+GPSL+ LADR+EGKLP+A ALL LR+ IL +I RATE R+ TPQ Sbjct: 301 EQVYFPQSEVIGDIGPSLKALADRLEGKLPNAHALLHLRERILERIAARATEDRF--TPQ 358 Query: 361 RIVHDVRQVIPEDGIVALDNGMYKIWFARNYRTLLANSLLLDNALATMGAGLPSAMMAAM 420 R+VHD+R+V+P DGI+ALDNGMYKIWFARNYRT +AN+LLLDNALATMGAGLPSAM+A+M Sbjct: 359 RLVHDIREVMPHDGILALDNGMYKIWFARNYRTRMANTLLLDNALATMGAGLPSAMVASM 418 Query: 421 LYPKNRVLAVCGDGGFMMNSQEMETAVRLKLNLVILILEDSAYGMIRWKQAVDHFPDFGL 480 LYP+ RV+A+CGDGGFMMNSQE+ETAVRLKLNLV+L++ED+AYGMIRWKQAVD FPDFG+ Sbjct: 419 LYPERRVMAICGDGGFMMNSQELETAVRLKLNLVVLVIEDNAYGMIRWKQAVDEFPDFGM 478 Query: 481 TFGNPDFVKYAESYGAKGSRITSTDAIVPTLERAFSGGGVHLVVAPIDYTENKRVLVDEL 540 TFGNPDFVKYAESYGA+G+R+ LE AFSGGGVHLV P+DY+EN+RVLV EL Sbjct: 479 TFGNPDFVKYAESYGARGTRVEDISQFKQVLEEAFSGGGVHLVNVPVDYSENERVLVKEL 538 Query: 541 REKVQQI 547 RE++ I Sbjct: 539 RERLPAI 545 >ref|NP_770663.1| acetolactate synthase [Bradyrhizobium japonicum USDA 110] dbj|BAC49288.1| blr4023 [Bradyrhizobium japonicum USDA 110] Length = 554 Score = 872 bits (2254), Expect = 0.0, Method: Compositional matrix adjust. Identities = 429/540 (79%), Positives = 482/540 (89%) Query: 2 VKGSDLLVAALENEGVERVFGVPGEENLDVVESLRKSSIKLIVTRHEQAAAFMAATYGRL 61 VKGSDL VAALENEGV+R+FGVPGEENLD+VESLR S I+LI+TRHEQAAAFMAAT+GRL Sbjct: 8 VKGSDLFVAALENEGVDRIFGVPGEENLDLVESLRTSKIELILTRHEQAAAFMAATHGRL 67 Query: 62 TGKPGVCMTTLGPGALNLTTGAAYALLGAMPMVMLTGQKGILSSRQAKFQIVDIVNTFRP 121 TGKPGVC++TLGPGALNL+TGAAYA LGAMPM+++TGQK I+SSRQA+FQIVD+V T +P Sbjct: 68 TGKPGVCLSTLGPGALNLSTGAAYAHLGAMPMILITGQKPIMSSRQARFQIVDVVATMKP 127 Query: 122 LTKLSLQIVSGATIPTLVRDAFRIATQERPGPVLLELPEDIAGEEIAPVEMISPHPIEIP 181 LTKLS QI+S ++IPT+VRDAFR+A +ERPGPV LELPEDIAG+E+ +I HPIEIP Sbjct: 128 LTKLSRQIISASSIPTVVRDAFRVAMEERPGPVHLELPEDIAGDEVPAAPVIPIHPIEIP 187 Query: 182 IAHAAALDRAADMILKAQRPLIMLGAAASRPRSTAGIGDFVQRTKIPFFTTQMGKGTVSG 241 +AH AALDRAA+MIL A+ PL+M+GAA SRPRST GI FV+RT IPFFTTQMGKGTV G Sbjct: 188 VAHRAALDRAAEMILAARHPLVMMGAATSRPRSTHGIASFVRRTGIPFFTTQMGKGTVPG 247 Query: 242 GTNQYMGTAALSERDYVHEAIDRADLIIAIGHDTVEKPPFIMGPKGPQVIHVSYMPATVE 301 GTN YMGTAALSERDYVH+AID ADLI+AIGHD +EKPPFIMGP GP+VIHVSY A+VE Sbjct: 248 GTNLYMGTAALSERDYVHDAIDAADLIVAIGHDPIEKPPFIMGPSGPKVIHVSYTSASVE 307 Query: 302 QVYFPQCEVIGDVGPSLELLADRVEGKLPHASALLSLRQGILAKITDRATEGRWPPTPQR 361 QVYFP EV+GDVGPSLELLADR+EGKLP A+ALL LR+ IL I DRATE RWPPTPQR Sbjct: 308 QVYFPDAEVVGDVGPSLELLADRLEGKLPQAAALLPLREEILRHIADRATEARWPPTPQR 367 Query: 362 IVHDVRQVIPEDGIVALDNGMYKIWFARNYRTLLANSLLLDNALATMGAGLPSAMMAAML 421 IVHD+RQVIPE+GIVALDNGMYKIWFARNYRT +AN+LLLDNALATMGAGLPSAMMAAML Sbjct: 368 IVHDIRQVIPENGIVALDNGMYKIWFARNYRTRVANTLLLDNALATMGAGLPSAMMAAML 427 Query: 422 YPKNRVLAVCGDGGFMMNSQEMETAVRLKLNLVILILEDSAYGMIRWKQAVDHFPDFGLT 481 YP RVLAV GDGGFMMNSQEMETAVRLKLNLV+L+LED+AYGMIRWKQAVDHF DFG+T Sbjct: 428 YPDRRVLAVAGDGGFMMNSQEMETAVRLKLNLVVLVLEDNAYGMIRWKQAVDHFADFGMT 487 Query: 482 FGNPDFVKYAESYGAKGSRITSTDAIVPTLERAFSGGGVHLVVAPIDYTENKRVLVDELR 541 FGNPDFV YA++YGAKG RI S D+ PTL+ AF GGVHLV PIDY+EN RVLVDELR Sbjct: 488 FGNPDFVLYAKAYGAKGHRIASIDSFGPTLDAAFREGGVHLVSIPIDYSENVRVLVDELR 547 >ref|YP_001755075.1| thiamine pyrophosphate binding domain-containing protein [Methylobacterium radiotolerans JCM 2831] gb|ACB24392.1| thiamine pyrophosphate protein domain protein TPP-binding [Methylobacterium radiotolerans JCM 2831] Length = 550 Score = 858 bits (2216), Expect = 0.0, Method: Compositional matrix adjust. Identities = 415/548 (75%), Positives = 490/548 (89%), Gaps = 1/548 (0%) Query: 1 MVKGSDLLVAALENEGVERVFGVPGEENLDVVESLRKSSIKLIVTRHEQAAAFMAATYGR 60 M KGSDLLVAALENEGV+R+FG+PGEENLDVVESLR S I+L++TRHEQAA+FMAAT+GR Sbjct: 1 MPKGSDLLVAALENEGVDRIFGIPGEENLDVVESLRNSKIELVLTRHEQAASFMAATHGR 60 Query: 61 LTGKPGVCMTTLGPGALNLTTGAAYALLGAMPMVMLTGQKGILSSRQAKFQIVDIVNTFR 120 LTG+PGVC++TLGPGALN +TGAAYA LGAMPM+++TGQKGILSSRQAKFQIVD++++ + Sbjct: 61 LTGRPGVCLSTLGPGALNFSTGAAYAHLGAMPMIIITGQKGILSSRQAKFQIVDVISSMK 120 Query: 121 PLTKLSLQIVSGATIPTLVRDAFRIATQERPGPVLLELPEDIAGEEIAPVEMISPHPIEI 180 P+TK++ QIVS ++IPT+VRDAFR+AT+ERPGPVLLELPEDIA EE A +++ HPI+I Sbjct: 121 PITKMARQIVSASSIPTIVRDAFRVATEERPGPVLLELPEDIASEE-AEADLVPSHPIDI 179 Query: 181 PIAHAAALDRAADMILKAQRPLIMLGAAASRPRSTAGIGDFVQRTKIPFFTTQMGKGTVS 240 P+AH A+ RAA+MILKA+RPLIMLGAAASRPR+T +G FVQRT IPFFTTQMGKGTV+ Sbjct: 180 PVAHPTAIARAAEMILKAERPLIMLGAAASRPRATGELGGFVQRTGIPFFTTQMGKGTVA 239 Query: 241 GGTNQYMGTAALSERDYVHEAIDRADLIIAIGHDTVEKPPFIMGPKGPQVIHVSYMPATV 300 GGT+ YMGTAALSERDYVHEAIDRADLIIAIGHDT+EKPPF MG +V+H+SYMPA V Sbjct: 240 GGTDLYMGTAALSERDYVHEAIDRADLIIAIGHDTIEKPPFFMGQADQKVVHISYMPANV 299 Query: 301 EQVYFPQCEVIGDVGPSLELLADRVEGKLPHASALLSLRQGILAKITDRATEGRWPPTPQ 360 EQVY+P EV+GD+GP+L+LLAD+++GKLP+A ALL LR+ IL+ I DRA E R+P TPQ Sbjct: 300 EQVYYPDAEVVGDLGPTLKLLADKLDGKLPNAGALLHLREHILSHIGDRAGEDRFPVTPQ 359 Query: 361 RIVHDVRQVIPEDGIVALDNGMYKIWFARNYRTLLANSLLLDNALATMGAGLPSAMMAAM 420 R+V DVR+VIPEDGIV LDNGMYKIWFARNYRT +AN+LLLDNALATMGAGLPSAMMAAM Sbjct: 360 RLVRDVRRVIPEDGIVCLDNGMYKIWFARNYRTYVANTLLLDNALATMGAGLPSAMMAAM 419 Query: 421 LYPKNRVLAVCGDGGFMMNSQEMETAVRLKLNLVILILEDSAYGMIRWKQAVDHFPDFGL 480 LYPK RV+AVCGDGGFMMNSQEMETAVRLKLNLV+L+L+DSAYGMIRWKQAVD F D+G+ Sbjct: 420 LYPKRRVMAVCGDGGFMMNSQEMETAVRLKLNLVVLVLDDSAYGMIRWKQAVDKFADYGM 479 Query: 481 TFGNPDFVKYAESYGAKGSRITSTDAIVPTLERAFSGGGVHLVVAPIDYTENKRVLVDEL 540 TF NPDFV YA+SYGA G R+ S + +VPTL+RAF+ GGVHLV PIDY+EN RVLVDEL Sbjct: 480 TFNNPDFVLYAKSYGATGHRVESVNDLVPTLDRAFAAGGVHLVAVPIDYSENTRVLVDEL 539 Query: 541 REKVQQID 548 R KV+ + Sbjct: 540 RAKVKDFE 547 >ref|YP_002973558.1| acetolactate synthase [Rhizobium leguminosarum bv. trifolii WSM1325] gb|ACS59597.1| thiamine pyrophosphate protein domain protein TPP-binding [Rhizobium leguminosarum bv. trifolii WSM1325] Length = 548 Score = 856 bits (2212), Expect = 0.0, Method: Compositional matrix adjust. Identities = 416/547 (76%), Positives = 482/547 (88%), Gaps = 2/547 (0%) Query: 1 MVKGSDLLVAALENEGVERVFGVPGEENLDVVESLRKSSIKLIVTRHEQAAAFMAATYGR 60 M KGSDLLVAALENEGV+R+FG+PGEENLDVVES+RKSSI+L++TRHEQAAAFMAATYGR Sbjct: 1 MTKGSDLLVAALENEGVDRIFGIPGEENLDVVESIRKSSIELVLTRHEQAAAFMAATYGR 60 Query: 61 LTGKPGVCMTTLGPGALNLTTGAAYALLGAMPMVMLTGQKGILSSRQAKFQIVDIVNTFR 120 LTGKPGVC+TTLGPGALNL+TGAAYALLGAMPMVM+TGQKGILSSRQA+FQ+VD+V + + Sbjct: 61 LTGKPGVCLTTLGPGALNLSTGAAYALLGAMPMVMITGQKGILSSRQARFQVVDVVASMK 120 Query: 121 PLTKLSLQIVSGATIPTLVRDAFRIATQERPGPVLLELPEDIAGEEIAPVEMISPHPIEI 180 PLTKL+ QIVS IPT VR+AFRIA +ERPGPV LELPEDIA EE V +I+PH +E+ Sbjct: 121 PLTKLARQIVSPQMIPTTVREAFRIAQEERPGPVHLELPEDIAAEECQEVALIAPHQLEL 180 Query: 181 PIAHAAALDRAADMILKAQRPLIMLGAAASRPRSTAGIGDFVQRTKIPFFTTQMGKGTVS 240 P A AALDRAA +I A+RPL+M GAAASRPRST+ I FV RT+IPFFTTQMGKGTV Sbjct: 181 PTASDAALDRAAALIAAAKRPLLMFGAAASRPRSTSDIAQFVIRTRIPFFTTQMGKGTVP 240 Query: 241 GGTNQYMGTAALSERDYVHEAIDRADLIIAIGHDTVEKPPFIMGPKGPQVIHVSYMPATV 300 GGT YMGTAALSERDYVHEAI++ADLII IGHDT+EKPPFIMG GP+V+H+ Y PATV Sbjct: 241 GGTELYMGTAALSERDYVHEAIEQADLIITIGHDTIEKPPFIMGKSGPKVVHIGYQPATV 300 Query: 301 EQVYFPQCEVIGDVGPSLELLADRVEGKLPHASALLSLRQGILAKITDRATEGRWPPTPQ 360 EQVYFPQ EVIGD+GPSL+ LADR+EGKLP+A ALL LR+ IL +I RATE R+ TPQ Sbjct: 301 EQVYFPQSEVIGDIGPSLKALADRLEGKLPNAQALLHLRERILERIATRATEDRF--TPQ 358 Query: 361 RIVHDVRQVIPEDGIVALDNGMYKIWFARNYRTLLANSLLLDNALATMGAGLPSAMMAAM 420 R+VHD+R+V+P DGI+ALDNGMYKIWFARNYRT +AN+LLLDNALATMGAGLPSAM+A+M Sbjct: 359 RLVHDIREVMPHDGILALDNGMYKIWFARNYRTRMANTLLLDNALATMGAGLPSAMVASM 418 Query: 421 LYPKNRVLAVCGDGGFMMNSQEMETAVRLKLNLVILILEDSAYGMIRWKQAVDHFPDFGL 480 LYP+ RV+A+CGDGGFMMNSQE+ETAVRLKLNLV+L++ED+AYGMIRWKQAVD FPDFG+ Sbjct: 419 LYPERRVMAICGDGGFMMNSQELETAVRLKLNLVVLVIEDNAYGMIRWKQAVDEFPDFGM 478 Query: 481 TFGNPDFVKYAESYGAKGSRITSTDAIVPTLERAFSGGGVHLVVAPIDYTENKRVLVDEL 540 TFGNPDFVKYAESYGAKG+R+ LE AF+GGGVHLV P+DY+EN+RVLV EL Sbjct: 479 TFGNPDFVKYAESYGAKGTRVDDIGQFKQVLEEAFAGGGVHLVNVPVDYSENERVLVKEL 538 Query: 541 REKVQQI 547 RE++ I Sbjct: 539 RERLPAI 545 >ref|YP_004144397.1| thiamine pyrophosphate TPP-binding domain-containing protein [Mesorhizobium ciceri biovar biserrulae WSM1271] gb|ADV14347.1| thiamine pyrophosphate TPP-binding domain-containing protein [Mesorhizobium ciceri biovar biserrulae WSM1271] Length = 548 Score = 855 bits (2208), Expect = 0.0, Method: Compositional matrix adjust. Identities = 416/544 (76%), Positives = 483/544 (88%), Gaps = 2/544 (0%) Query: 1 MVKGSDLLVAALENEGVERVFGVPGEENLDVVESLRKSSIKLIVTRHEQAAAFMAATYGR 60 M KGSDL VAALENEGVER+FG+PGEENLD+VES+R+SSI+LI+TRHEQAAAFMAATYGR Sbjct: 1 MTKGSDLFVAALENEGVERIFGIPGEENLDIVESIRRSSIQLILTRHEQAAAFMAATYGR 60 Query: 61 LTGKPGVCMTTLGPGALNLTTGAAYALLGAMPMVMLTGQKGILSSRQAKFQIVDIVNTFR 120 LTGKPGVC+TTLGPGALNL+TG+AYALLGAMPM+M+TGQKGILSSRQA+FQIVDIV + Sbjct: 61 LTGKPGVCITTLGPGALNLSTGSAYALLGAMPMIMITGQKGILSSRQARFQIVDIVAAMK 120 Query: 121 PLTKLSLQIVSGATIPTLVRDAFRIATQERPGPVLLELPEDIAGEEIAPVEMISPHPIEI 180 PLTKLS QIVS IP+LVR+AFR+A +ERPGPV LELPEDIA E PV ++ HP+E+ Sbjct: 121 PLTKLSRQIVSPKMIPSLVREAFRVAQEERPGPVHLELPEDIAAAECEPVALVPTHPVEL 180 Query: 181 PIAHAAALDRAADMILKAQRPLIMLGAAASRPRSTAGIGDFVQRTKIPFFTTQMGKGTVS 240 P+A ALDRAA MI++A+RPL+M GAAASRPR T + FV RT+IP+FTTQMGKGTV Sbjct: 181 PLASPGALDRAARMIMEARRPLLMFGAAASRPRVTPDVAQFVLRTQIPYFTTQMGKGTVP 240 Query: 241 GGTNQYMGTAALSERDYVHEAIDRADLIIAIGHDTVEKPPFIMGPKGPQVIHVSYMPATV 300 GGT YMGTAALSERDYVHEAI++ADLII IGHDTVEKPPFIMG KGP+VIHV Y A V Sbjct: 241 GGTELYMGTAALSERDYVHEAIEQADLIITIGHDTVEKPPFIMGAKGPKVIHVGYHSADV 300 Query: 301 EQVYFPQCEVIGDVGPSLELLADRVEGKLPHASALLSLRQGILAKITDRATEGRWPPTPQ 360 EQVYFPQ E++GD+GPSL LLADR+EGK+P+A ALL LR+GIL++I RATE R+ TPQ Sbjct: 301 EQVYFPQAEIVGDLGPSLALLADRIEGKVPNAQALLPLREGILSRIAARATEDRF--TPQ 358 Query: 361 RIVHDVRQVIPEDGIVALDNGMYKIWFARNYRTLLANSLLLDNALATMGAGLPSAMMAAM 420 RIVHDVR V+P DGI+ALDNGMYKIWFARNYRT +AN++LLDNALATMGAGLPSAMMAA+ Sbjct: 359 RIVHDVRAVMPPDGILALDNGMYKIWFARNYRTRMANTILLDNALATMGAGLPSAMMAAL 418 Query: 421 LYPKNRVLAVCGDGGFMMNSQEMETAVRLKLNLVILILEDSAYGMIRWKQAVDHFPDFGL 480 LYP+ RV+A+CGDGGFMMNSQE+ETAVRLKLNLV+L+LED AYGMIRWKQAVD FPDFG+ Sbjct: 419 LYPQRRVMAICGDGGFMMNSQELETAVRLKLNLVVLLLEDHAYGMIRWKQAVDEFPDFGM 478 Query: 481 TFGNPDFVKYAESYGAKGSRITSTDAIVPTLERAFSGGGVHLVVAPIDYTENKRVLVDEL 540 TFGNPDFV+YAE+YGA+G+R+T + P LE+AF+GGGVHLVV PIDY+EN RVLVDEL Sbjct: 479 TFGNPDFVRYAEAYGARGTRVTEIADLRPALEQAFTGGGVHLVVVPIDYSENTRVLVDEL 538 Query: 541 REKV 544 RE++ Sbjct: 539 RERL 542