BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0031.p (644 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_772505.1| multidrag resistance protein [Bradyrhizobium ja... 486 e-135 ref|YP_556542.1| major facilitator superfamily multidrug efflux ... 287 5e-75 ref|YP_999298.1| EmrB/QacA family drug resistance transporter [V... 282 2e-73 ref|YP_003643724.1| major facilitator superfamily MFS_1 [Thiomon... 281 3e-73 ref|YP_004751324.1| major facilitator superfamily (MFS) multidru... 280 4e-73 >ref|NP_772505.1| multidrag resistance protein [Bradyrhizobium japonicum USDA 110] dbj|BAC51130.1| bll5865 [Bradyrhizobium japonicum USDA 110] Length = 469 Score = 486 bits (1251), Expect = e-135, Method: Compositional matrix adjust. Identities = 252/334 (75%), Positives = 283/334 (84%) Query: 37 NVRHMPPDRRAASHQQSSGLGATLTLAAMSLGYGVVQLDVTIVNTALNAIGASLGGGVAE 96 +++ D A + + SS +LTLAAMSLGYGVVQLDVTIVNTAL+A+G +LGGGVAE Sbjct: 5 TIKYEAFDDGAGAPRASSRPATSLTLAAMSLGYGVVQLDVTIVNTALDAMGRTLGGGVAE 64 Query: 97 LQWVVSAYTIAFAAFILTAGALGDRIGAKRIFMAGFAIFTAASVGCAFAPDAATLIAARC 156 LQWVVSAYTIAFAAFILTAGALGDRIGAKR+FMAGFAIFTAAS+ CA +P+A LIAAR Sbjct: 65 LQWVVSAYTIAFAAFILTAGALGDRIGAKRVFMAGFAIFTAASLACASSPNAIVLIAARL 124 Query: 157 VQGLAAAILVPNSLALLRHAYEDEQARGRAVGVWAAGASLALTAGPFVGGGLIALVGWRA 216 VQGLAAAILVPNSLALL HAY D++ARGRAV +WAAGASLALTAGPFVGG LI LVGWRA Sbjct: 125 VQGLAAAILVPNSLALLNHAYADDRARGRAVAIWAAGASLALTAGPFVGGALITLVGWRA 184 Query: 217 IFLVNLPIGLAGLWLAWRFAGETARHQQHRLDLPGQVAAIAALGTLAGALIEGGALGWGS 276 IFLVNLPIGL GLWL+WR+A ET R +DLPGQ+ AI ALG LAGA+IEGGALGW Sbjct: 185 IFLVNLPIGLMGLWLSWRYATETTRAPSREIDLPGQLTAIGALGALAGAIIEGGALGWDH 244 Query: 277 PFVIAGFALAAVFSVLFVWREARAPQPMLPLSLFRHRMFALTSLVGLLVNVAFYGLIFVL 336 P V+ FA A V +VLFVWRE+RA QPMLPLSLF HR+F LT+LVGLLVN+A YGLIFVL Sbjct: 245 PAVLVAFASALVLAVLFVWRESRAAQPMLPLSLFGHRLFTLTALVGLLVNIAIYGLIFVL 304 Query: 337 SLYFQQVNGLSALATGLAFVPMLGAVLPANLIAP 370 SLYFQ++NGLSA TGLAFVPM+ AVLP NL+AP Sbjct: 305 SLYFQRINGLSAWWTGLAFVPMMAAVLPVNLLAP 338 >ref|YP_556542.1| major facilitator superfamily multidrug efflux transporter [Burkholderia xenovorans LB400] gb|ABE37192.1| major facilitator superfamily (MFS) multidrug efflux pump, EmrB/QacA subfamily [Burkholderia xenovorans LB400] Length = 469 Score = 287 bits (734), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 168/309 (54%), Positives = 212/309 (68%) Query: 61 TLAAMSLGYGVVQLDVTIVNTALNAIGASLGGGVAELQWVVSAYTIAFAAFILTAGALGD 120 TLAA LG+ VV LDV++VN AL+A+ A V LQWVV+AYT+AFA+ +LT GALGD Sbjct: 12 TLAAAGLGFVVVLLDVSVVNVALDALRARFATDVTGLQWVVNAYTLAFASLLLTTGALGD 71 Query: 121 RIGAKRIFMAGFAIFTAASVGCAFAPDAATLIAARCVQGLAAAILVPNSLALLRHAYEDE 180 R GAKRIF AGFA+FT AS+GC A L+AAR VQG+ AA+LVPNSLA+LR A+ D Sbjct: 72 RFGAKRIFTAGFAVFTLASIGCGIADSLPLLVAARVVQGIGAALLVPNSLAMLRQAFPDM 131 Query: 181 QARGRAVGVWAAGASLALTAGPFVGGGLIALVGWRAIFLVNLPIGLAGLWLAWRFAGETA 240 Q R RAVG W A +AL AGP +GG L+ VGWR+IFL+NLPIG+AG+ L R A Sbjct: 132 QQRSRAVGWWGAAGGIALAAGPVLGGLLVTHVGWRSIFLINLPIGVAGVALTLRHAAAAV 191 Query: 241 RHQQHRLDLPGQVAAIAALGTLAGALIEGGALGWGSPFVIAGFALAAVFSVLFVWREARA 300 +DLPGQ+AAI AL L L E LGW P V L+ V ++ F+W E+R+ Sbjct: 192 STHNRSIDLPGQLAAIVALVALTVCLTEAARLGWRHPLVYGALLLSVVATLAFLWLESRS 251 Query: 301 PQPMLPLSLFRHRMFALTSLVGLLVNVAFYGLIFVLSLYFQQVNGLSALATGLAFVPMLG 360 +PMLPLSLFR F++ S+ G++VN A+YGLIFV SL+FQ LSA TGLAF+PM Sbjct: 252 TEPMLPLSLFRTATFSVASVAGVIVNFAYYGLIFVFSLFFQIEQNLSAQWTGLAFLPMTA 311 Query: 361 AVLPANLIA 369 ++ N++A Sbjct: 312 VLMGVNILA 320 >ref|YP_999298.1| EmrB/QacA family drug resistance transporter [Verminephrobacter eiseniae EF01-2] gb|ABM60280.1| drug resistance transporter, EmrB/QacA subfamily [Verminephrobacter eiseniae EF01-2] Length = 452 Score = 282 bits (721), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 155/309 (50%), Positives = 205/309 (66%) Query: 61 TLAAMSLGYGVVQLDVTIVNTALNAIGASLGGGVAELQWVVSAYTIAFAAFILTAGALGD 120 LAA S+ Y VV LD +IVN AL I +L ++ LQWVV+AYT+AFA +L+ G LGD Sbjct: 11 VLAATSISYIVVILDTSIVNVALERISNALHTHISGLQWVVNAYTLAFACLLLSGGTLGD 70 Query: 121 RIGAKRIFMAGFAIFTAASVGCAFAPDAATLIAARCVQGLAAAILVPNSLALLRHAYEDE 180 R GA+ I++AG A+FTAAS C FAPD L AR +QG+ AA+LVP SL L+ HAY D Sbjct: 71 RWGARNIYLAGLALFTAASALCGFAPDLTILTIARVLQGVGAALLVPCSLTLINHAYPDP 130 Query: 181 QARGRAVGVWAAGASLALTAGPFVGGGLIALVGWRAIFLVNLPIGLAGLWLAWRFAGETA 240 R A GVWA+ A+ AGP VGG LI + GWR+IFL N+PIGL G+WL WR A + + Sbjct: 131 GERAVAFGVWASCGGAAMAAGPLVGGLLINMFGWRSIFLANVPIGLLGVWLTWRVARDKS 190 Query: 241 RHQQHRLDLPGQVAAIAALGTLAGALIEGGALGWGSPFVIAGFALAAVFSVLFVWREARA 300 Q LDL GQ++ I ALG+L LIEG LGW S ++ G A++ LF+ E+ Sbjct: 191 EAQIRHLDLSGQLSLIVALGSLITVLIEGPVLGWQSLPILIGIAVSIASGGLFLVIESLR 250 Query: 301 PQPMLPLSLFRHRMFALTSLVGLLVNVAFYGLIFVLSLYFQQVNGLSALATGLAFVPMLG 360 QPMLPLS F + +F+ + +V ++ + FYGL+F+LSLYFQQV SAL TGL+F+P+ Sbjct: 251 DQPMLPLSFFGNGLFSGSVVVTMVSALIFYGLVFMLSLYFQQVRNYSALQTGLSFLPLTA 310 Query: 361 AVLPANLIA 369 V ++I+ Sbjct: 311 MVTFGSMIS 319 >ref|YP_003643724.1| major facilitator superfamily MFS_1 [Thiomonas intermedia K12] gb|ADG31394.1| major facilitator superfamily MFS_1 [Thiomonas intermedia K12] Length = 472 Score = 281 bits (719), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 152/313 (48%), Positives = 211/313 (67%), Gaps = 4/313 (1%) Query: 61 TLAAMSLGYGVVQLDVTIVNTALNAIGASLGGGVAELQWVVSAYTIAFAAFILTAGALGD 120 TL A +LG+ VV LDV+ VN AL A+ VA LQWVV+AYT+ FAA +LT+G+LGD Sbjct: 21 TLIAAALGFVVVLLDVSAVNVALGALHRDFATAVAGLQWVVNAYTLVFAALLLTSGSLGD 80 Query: 121 RIGAKRIFMAGFAIFTAASVGCAFAPDAATLIAARCVQGLAAAILVPNSLALLRHAYEDE 180 R+G++R+F+ GF +FT AS C+ A + L+ AR V+GL A++LVPNSLA+L+ A+ D+ Sbjct: 81 RLGSRRVFLCGFTLFTLASAACSVAINLPMLVVARLVEGLGASLLVPNSLAILKRAFPDK 140 Query: 181 QARGRAVGVWAAGASLALTAGPFVGGGLIALVGWRAIFLVNLPIGLAGLWLAWRF-AGET 239 R RAVG W A ++L AGP +GG L+ GWR+IFL+NLPIGL G +L ++ A + Sbjct: 141 LQRSRAVGWWGAIGGISLAAGPVLGGLLVTHWGWRSIFLINLPIGLVGGYLTLQYVAADG 200 Query: 240 ARHQQHR---LDLPGQVAAIAALGTLAGALIEGGALGWGSPFVIAGFALAAVFSVLFVWR 296 +RH +H LD PGQ+ AI +L AL E G LGWGS +V G LA V + F+W Sbjct: 201 SRHPRHHDDGLDWPGQLVAILSLAAFTAALTEAGQLGWGSVWVQGGLLLAVVAAAGFIWI 260 Query: 297 EARAPQPMLPLSLFRHRMFALTSLVGLLVNVAFYGLIFVLSLYFQQVNGLSALATGLAFV 356 E+R+ PMLPL LFR F + ++ G++VN ++YGLIF+ SL+FQ LS TG+AF+ Sbjct: 261 ESRSRSPMLPLGLFRVSAFTIATISGVIVNFSYYGLIFIFSLFFQIEQQLSPQQTGMAFL 320 Query: 357 PMLGAVLPANLIA 369 PM ++ N++A Sbjct: 321 PMTIVLMGVNVLA 333 >ref|YP_004751324.1| major facilitator superfamily (MFS) multidrug efflux pump [Collimonas fungivorans Ter331] gb|AEK60501.1| major facilitator superfamily (MFS) multidrug efflux pump, EmrB/QacA subfamily [Collimonas fungivorans Ter331] Length = 490 Score = 280 bits (717), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 164/310 (52%), Positives = 206/310 (66%) Query: 60 LTLAAMSLGYGVVQLDVTIVNTALNAIGASLGGGVAELQWVVSAYTIAFAAFILTAGALG 119 LTL A SLG +V LDVT+VN AL I ASL V LQW+++AYT+ FA+ +LTAGA+G Sbjct: 30 LTLIATSLGLVLVTLDVTVVNVALERIQASLSTNVTGLQWIINAYTLVFASLLLTAGAIG 89 Query: 120 DRIGAKRIFMAGFAIFTAASVGCAFAPDAATLIAARCVQGLAAAILVPNSLALLRHAYED 179 DR GAKRIF+ GFA+FT AS+ C A TLIA R VQG+ AA+ VP SLALL ++ + Sbjct: 90 DRFGAKRIFVIGFALFTIASLACGMAGTIGTLIAGRVVQGVGAALCVPASLALLSASFPE 149 Query: 180 EQARGRAVGVWAAGASLALTAGPFVGGGLIALVGWRAIFLVNLPIGLAGLWLAWRFAGET 239 +R RAV +WA LAL AGP VGG ++ GW +IF +NLP+GL G+WL +A Sbjct: 150 AASRARAVSIWAGVGGLALAAGPVVGGIMVDRFGWPSIFFLNLPLGLLGIWLTLSYAPSG 209 Query: 240 ARHQQHRLDLPGQVAAIAALGTLAGALIEGGALGWGSPFVIAGFALAAVFSVLFVWREAR 299 ++ LDL GQV AI ALG L +E GALGW P V++GF V LF+ EAR Sbjct: 210 SKTPNQGLDLAGQVLAILALGGLTLGFVEAGALGWTHPLVLSGFTCFLVAGALFLLAEAR 269 Query: 300 APQPMLPLSLFRHRMFALTSLVGLLVNVAFYGLIFVLSLYFQQVNGLSALATGLAFVPML 359 PMLPLSLFR + + + +VGLL N A+YGL+FVLSL+FQ G S L TGLAF+PM Sbjct: 270 GADPMLPLSLFRSSVVSASCVVGLLTNFAYYGLMFVLSLFFQATKGYSPLMTGLAFLPMT 329 Query: 360 GAVLPANLIA 369 V ANL++ Sbjct: 330 ALVTIANLVS 339